@article{moore_ferket_mozdziak_2005, title={Early post-hatch fasting induces satellite cell self-renewal}, volume={142}, ISSN={["1531-4332"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-27644486955&partnerID=MN8TOARS}, DOI={10.1016/j.cbpa.2005.08.007}, abstractNote={Early post-hatch satellite cell kinetics are an important aspect of muscle development, and understanding the interplay between fasting and muscle development will lead to improvements in muscle mass following an illness, and optimal meat production. The objective of this experiment was to test the influence of immediate post-hatch fasting on satellite cells in the poult. Male Nicholas poults (Meleagris gallopavo) were placed into two treatments: a fed treatment with immediate access to feed and water upon placement and a fasted treatment without access to feed and water for the first three days post-hatch. 5-bromo-2'-deoxyuridine (BrdU) was injected intra-abdominally in all poults to label mitotically active satellite cells. The pectoralis thoracicus muscle was harvested two hours following the BrdU injection. Immunohistochemistry for BrdU, Pax7, Bcl-2, Pax7 with BrdU, and determining myofiber cross-sectional area along with computer-based image analysis was used to study muscle development. Fed poults had higher body masses throughout the experiment (P< or =0.01), and they had higher pectoralis thoracicus muscle mass (P< or =0.01) at ten days of age than the fasted poults. Fed poults had higher satellite cell mitotic activity at three days and four days of age (P< or =0.01) compared to the fasted poults. However, Pax7 labeling index was higher in the fasted poults (P< or =0.01) at three days, four days, and five days post-hatch than the fed group. Similarly Bcl-2 labeling was higher in the fasted than in the fed group at three days post-hatch. Therefore, fasting depleted proliferating satellite cells indicated by the lower BrdU labeling in the fasted poults compared to the fed poults, and conserved the satellite cell proliferative reserve indicated by the higher level of Pax7 labeling for the fasted poults compared to the fed poults.}, number={3}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY}, author={Moore, DT and Ferket, PR and Mozdziak, PE}, year={2005}, month={Nov}, pages={331–339} } @article{moore_ferket_mozdziak_2005, title={Muscle development in the late embryonic and early post-hatch poult}, volume={4}, ISBN={1682-8356}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-34548478053&partnerID=MN8TOARS}, DOI={10.3923/ijps.2005.138.142}, number={3}, journal={International Journal of Poultry Science}, author={Moore, D.T. and Ferket, P.R. and mozdziak}, year={2005}, pages={138–142} } @article{moore_ferket_mozdziak_2005, title={The effect of early nutrition on satellite cell dynamics in the young turkey}, volume={84}, ISSN={["1525-3171"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-19044397721&partnerID=MN8TOARS}, DOI={10.1093/ps/84.5.748}, abstractNote={Early posthatch satellite cell mitotic activity is an important aspect of muscle development. An understanding of the interplay between nutrition and satellite cell mitotic activity will lead to more efficient meat production. The objective of this study was to test the influence of the leucine metabolite, beta-hydroxy beta-methylbutyrate (HMB), and feed deprivation on muscle development in the early posthatch poult. Male Nicholas poults were placed on 1 of 4 treatments: immediately fed a starter diet with 0.1% HMB (IF-HMB), immediately fed a starter diet containing 0.1% Solka-Floc for a control (IF-No HMB), feed and water withheld for 48 h immediately posthatch and then fed the HMB diet (WF-HMB), and feed and water withheld for 48 h immediately posthatch and then fed the control starter diet (WF-No HMB). 5-bromo-2'-deoxyuridine (BrdU) was injected intra-abdominally into all poults to label mitotically active satellite cells. The pectoralis thoracicus was harvested 2 h after the BrdU injection. Immunohistochemistry for BrdU, Pax7, and laminin along with computer-based image analysis was used to study muscle development. IF-HMB poults had higher body weights (P < 0.01) at 48 h and 1 wk of age and had higher satellite cell mitotic activity at 48 h of age (P < 0.01) compared with the IF-No HMB and WF poults. Therefore, dietary supplementation of HMB may have an anabolic effect on early posthatch muscle.}, number={5}, journal={POULTRY SCIENCE}, author={Moore, DT and Ferket, PR and Mozdziak, PE}, year={2005}, month={May}, pages={748–756} } @article{moore_ferket_mozdziak_2004, title={In ovo intraperitoneal administration of bromodeoxyuridine to avian fetuses}, volume={36}, ISSN={["1940-9818"]}, DOI={10.2144/04361BM05}, abstractNote={The embryological development of skeletal muscle begins with the fusion of mononucleated myoblasts that form myotubes, which then mature into myo-fibers (1). By late embryogenesis, myo-blasts found in the chicken fetus may be referred to as satellite cells, and they have predominantly adult characteristics (2). Satellite cells are located underneath the myofiber basal lamina along the entire length of the myofiber (3). Satellite cells are a mitotically active cell population (4), making it possible to label the cells with}, number={1}, journal={BIOTECHNIQUES}, author={Moore, DT and Ferket, PR and Mozdziak, PE}, year={2004}, month={Jan}, pages={50-+} } @article{moore_mozdziak_2004, title={The dynamics of skeletal muscle development}, volume={20}, ISBN={1388-3119}, journal={World Poultry (Doetinchem, Netherlands)}, author={Moore, D. T. and Mozdziak, P. E.}, year={2004}, pages={6} }