Elizabeth G. Loboa

Works (71)

Updated: August 22nd, 2023 21:22

2021 article

The evaluation of a multiphasic 3D-bioplotted scaffold seeded with adipose derived stem cells to repair osteochondral defects in a porcine model

Nordberg, R. C., Huebner, P., Schuchard, K. G., Mellor, L. F., Shirwaiker, R. A., Loboa, E. G., & Spang, J. T. (2021, June 10). JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, Vol. 6.

By: R. Nordberg n, P. Huebner  n, K. Schuchard n, L. Mellor n, R. Shirwaiker n , E. Loboa*, J. Spang*

author keywords: in vivo; osteochondral; stem cells; tissue engineering; 3D-printing
MeSH headings : Animals; Cartilage, Articular / injuries; Stem Cells; Swine; Swine, Miniature; Tissue Engineering / methods; Tissue Scaffolds / chemistry
TL;DR: Overall, scaffold groups repaired the defect more successfully than an open lesion but achieved limited repair in the cartilage region, indicating that this approach holds potential to treat focal cartilage lesions in a highly personalized manner using a human patient's own ASC cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: June 21, 2021

2020 journal article

Developing Implantable Scaffolds to Enhance Neural Stem Cell Therapy for Post-Operative Glioblastoma

MOLECULAR THERAPY, 28(4), 1056–1067.

By: K. Sheets*, M. Ewend*, M. Mohiti-Asli n, S. Tuin n, E. Loboa*, K. Aboody*, S. Hingtgen *

MeSH headings : Animals; Brain Neoplasms / pathology; Brain Neoplasms / surgery; Brain Neoplasms / therapy; Cell Line, Tumor; Combined Modality Therapy; Ganciclovir / administration & dosage; Ganciclovir / pharmacology; Glioblastoma / pathology; Glioblastoma / surgery; Glioblastoma / therapy; Humans; Luminescent Measurements; Mice; Neural Stem Cells / metabolism; Neural Stem Cells / transplantation; Polyesters / chemistry; Prodrugs / administration & dosage; Prodrugs / pharmacology; Thymidine Kinase / metabolism; Tissue Scaffolds / chemistry; Treatment Outcome; Xenograft Model Antitumor Assays
TL;DR: Design parameters for effective scaffold/tNSC composites are defined and suggest a new approach to maximizing the efficacy of tNSC therapy in human patient trials. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: April 27, 2020

2020 journal article

Investigation of multiphasic 3D-bioplotted scaffolds for site-specific chondrogenic and osteogenic differentiation of human adipose-derived stem cells for osteochondral tissue engineering applications

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 108(5), 2017–2030.

By: L. Mellor n, R. Nordberg n, P. Huebner  n, M. Mohiti-Asli n, M. Taylor n, W. Efird*, J. Oxford *, J. Spang*, R. Shirwaiker n , E. Loboa*

author keywords: 3D-printing; chondrogenic differentiation; human adipose derived stem cells; osteochondral; osteogenic differentiation
MeSH headings : Adipose Tissue / metabolism; Biocompatible Materials / chemistry; Bone and Bones; Calcium Phosphates / chemistry; Calcium Phosphates / metabolism; Cartilage, Articular / metabolism; Cell Differentiation; Cells, Cultured; Chondrogenesis / physiology; Collagen Type I / genetics; Collagen Type I / metabolism; Extracellular Matrix / chemistry; Extracellular Matrix / metabolism; Humans; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Osteogenesis / physiology; Polyesters / chemistry; Polyesters / metabolism; Printing, Three-Dimensional; Tissue Engineering; Tissue Scaffolds / chemistry
TL;DR: A scaffold that would induce site‐specific osteogenic and chondrogenic differentiation of human adipose‐derived stem cells (hASC) to generate a full osteochondral implant is created and holds great promise as cartilage morbidity is minimized since autologous cartilage harvest is not required. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: January 6, 2020

2019 journal article

Evidence for Aryl hydrocarbon Receptor-Mediated Inhibition of Osteoblast Differentiation in Human Mesenchymal Stem Cells

TOXICOLOGICAL SCIENCES, 167(1), 145–156.

By: A. Watson n, R. Nordberg n, E. Loboa n & S. Kullman n 

author keywords: aryl hydrocarbon receptor; 2,3,7,8-tetrachlorodibenzo-p-dioxin; TCDD; mesenchymal stem cell; osteoblast differentiation; skeletal toxicity; bone toxicity
MeSH headings : Basic Helix-Loop-Helix Transcription Factors / agonists; Cell Differentiation / drug effects; Fibroblast Growth Factor 9 / genetics; Fibroblast Growth Factors / genetics; Humans; Ligands; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / drug effects; Mesenchymal Stem Cells / metabolism; Osteoblasts / cytology; Osteoblasts / drug effects; Osteoblasts / metabolism; Osteogenesis / drug effects; Osteogenesis / genetics; Polychlorinated Dibenzodioxins / toxicity; Receptors, Aryl Hydrocarbon / agonists; Up-Regulation
TL;DR: Experimental evidence from this study suggests that AhR transactivation likely attenuates osteoblast differentiation in multipotent hBMSCs, and underscores the use of primary human MSCs to evaluate osteoinductive or osteotoxic potential of chemical and pharmacologic agents in vitro. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: April 15, 2019

2019 journal article

Industrial-scale fabrication of an osteogenic and antibacterial PLA/silver-loaded calcium phosphate composite with significantly reduced cytotoxicity

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 107(4), 900–910.

By: S. Cai n, B. Pourdeyhimi n  & E. Loboa*

author keywords: industrial-scale fabrication; multifunctional scaffold; osteogenic; antibacterial; reduced cytotoxicity
MeSH headings : Adipose Tissue / cytology; Adipose Tissue / metabolism; Anti-Bacterial Agents / chemical synthesis; Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacology; Calcium Phosphates / chemistry; Calcium Phosphates / pharmacology; Cell Differentiation / drug effects; Escherichia coli / growth & development; Humans; Osteogenesis / drug effects; Polyesters / chemical synthesis; Polyesters / chemistry; Polyesters / pharmacology; Silver / chemistry; Silver / pharmacology; Staphylococcus aureus / growth & development; Stem Cells / cytology; Stem Cells / metabolism; Tissue Scaffolds / chemistry
TL;DR: The results of this study indicate a promising alternative approach to produce multifunctional scaffolds at industrial-scale with higher throughput, lower cost, and enhanced reproducibility. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: April 22, 2019

2019 journal article

Primary Cilia Exhibit Mechanosensitivity to Cyclic Tensile Strain and Lineage-Dependent Expression in Adipose-Derived Stem Cells

SCIENTIFIC REPORTS, 9.

By: J. Bodle n, M. Hamouda n, S. Cai n, R. Williams n, S. Bernacki n & E. Loboa*

MeSH headings : Adipocytes / cytology; Adipocytes / metabolism; Adipogenesis / genetics; Cell Differentiation / genetics; Cell Lineage / genetics; Cells, Cultured; Cilia / genetics; Core Binding Factor Alpha 1 Subunit / genetics; Gene Expression Regulation, Developmental / genetics; Humans; Mechanotransduction, Cellular / genetics; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Osteogenesis / genetics; Tensile Strength; Tissue Engineering
TL;DR: This study demonstrates that hASC primary cilia exhibit mechanosensitivity to cyclic tensile strain and lineage-dependent expression, which may in part regulate signaling pathways localized to the primary cilium during the differentiation process. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: June 17, 2019

2018 article

Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain

ADIPOSE-DERIVED STEM CELLS: METHODS AND PROTOCOLS, 2ND EDITION, Vol. 1773, pp. 215–230.

By: R. Nordberg  n, J. Bodle n & E. Loboa*

author keywords: Adipose-derived stem cells; Tissue engineering; Mechanical stimulation; Tensile strain; Hydrostatic pressure; Microgravity; Differentiation; Osteogenesis; Chondrogenesis; Adipogenesis; Protocols; Methods
MeSH headings : Adipose Tissue / cytology; Cell Differentiation; Chondrogenesis; Humans; Hydrostatic Pressure; Mesenchymal Stem Cells / cytology; Musculoskeletal System; Osteogenesis; Primary Cell Culture; Stress, Mechanical; Tensile Strength; Tissue Engineering / methods; Weightlessness Simulation
TL;DR: This chapter describes methods for applying mechanical stimuli to hASC to direct differentiation for functional tissue engineering of the musculoskeletal system. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: October 16, 2018

2017 journal article

Creation and Evaluation of New Porcine Model for Investigation of Treatments of Surgical Site Infection

TISSUE ENGINEERING PART C-METHODS, 23(11), 795–803.

By: M. Mohiti-Asli n, M. Risselada n, M. Jacob n, B. Pourdeyhimi n  & E. Loboa n

author keywords: surgical site infection; porcine model; antimicrobial scaffold; silver; electrospinning; MRSA
MeSH headings : Animals; Anti-Infective Agents / pharmacology; Anti-Infective Agents / therapeutic use; Disease Models, Animal; Female; Methicillin-Resistant Staphylococcus aureus / drug effects; Nanofibers / chemistry; Nanofibers / ultrastructure; Polyesters / chemistry; Porosity; Surgical Wound Infection / drug therapy; Surgical Wound Infection / microbiology; Surgical Wound Infection / pathology; Sus scrofa; Tissue Scaffolds / chemistry
TL;DR: A porcine full-thickness incision model to investigate SSI caused by methicillin-resistant Staphylococcus aureus is developed, and the potential of silver releasing nanofibrous scaffolds for treatment/prevention of SSI is confirmed, and a new porcines model is introduced for in vivo evaluation of additional SSI treatment approaches are introduced. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Effects of 3D-bioplotted polycaprolactone scaffold geometry on human adipose-derived stem cell viability and proliferation

RAPID PROTOTYPING JOURNAL, 23(3), 534–542.

By: S. Mehendale n, L. Mellor n, M. Taylor n, E. Loboa n & R. Shirwaiker n 

Contributors: S. Mehendale n, L. Mellor n, M. Taylor n, E. Loboa n & R. Shirwaiker n 

author keywords: 3D-Bioplotting; Human adipose-derived stem cells; Polycaprolactone; Tissue engineering scaffolds
TL;DR: The design with the lowest porosity had the highest compression modulus after 14 days of culture, but resulted in the lowest hASC viability, which provided the basis for future studies which will use this optimal scaffold design to develop constructs for hASC-based osteochondral tissue engineering applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

Electrical Cell-Substrate Impedance Spectroscopy Can Monitor Age-Grouped Human Adipose Stem Cell Variability During Osteogenic Differentiation

STEM CELLS TRANSLATIONAL MEDICINE, 6(2), 502–511.

By: R. Nordberg n, J. Zhang n, E. Griffith n, M. Frank  n, B. Starly n  & E. Loboa n

author keywords: Electrical cell-substrate impedance spectroscopy; Adipose stem cells; Osteogenesis; Bioimpedance
MeSH headings : Adipose Tissue / cytology; Adult; Age Factors; Aged; Aged, 80 and over; Bone and Bones / cytology; Cell Differentiation; Cell Proliferation; Cells, Cultured; Dielectric Spectroscopy / methods; Electric Impedance; Humans; Middle Aged; Osteogenesis; Phenotype; Stem Cells / metabolism; Stem Cells / physiology; Time Factors; Young Adult
TL;DR: This is the first study to use ECIS to predict osteogenic potential of multiple hASC populations and to show that donor age may temporally control onset of osteogenesis, which could be critical for development of patient‐specific bone tissue engineering and regenerative medicine therapies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 105(2), 327–339.

By: M. Mohiti-Asli n, S. Saha n, S. Murphy *, H. Gracz n, B. Pourdeyhimi n , A. Atala*, E. Loboa n

author keywords: controlled release; drug delivery/release; inflammation; PLLA; wound healing
MeSH headings : Animals; Dermis / injuries; Dermis / metabolism; Dermis / pathology; Drug Carriers / chemistry; Drug Carriers / pharmacokinetics; Drug Carriers / pharmacology; Fibroblasts / metabolism; Fibroblasts / pathology; Humans; Ibuprofen / chemistry; Ibuprofen / pharmacokinetics; Ibuprofen / pharmacology; Keratinocytes / metabolism; Keratinocytes / pathology; Mice, Nude; Nanofibers / chemistry; Nanofibers / therapeutic use; Polyesters / chemistry; Polyesters / pharmacokinetics; Polyesters / pharmacology; Wound Healing / drug effects
TL;DR: It is found that degradable anti-inflammatory scaffolds containing 20 wt % ibuprofen promote human skin cell viability and proliferation in vitro, reduce wound contraction in vivo, and when seeded with skin cells, also enhance new blood vessel formation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

A Novel Ultrasound Technique for Non-Invasive Assessment of Cell Differentiation

IEEE SENSORS JOURNAL, 16(1), 61–68.

By: W. Huang *, J. Kim n, K. Kim n, S. Bakshi n, J. Williams n, P. Matthieu n, E. Loboa n, K. Shung*, Q. Zhou *, X. Jiang n

author keywords: Lead magnesium niobate-lead titanate piezoelectric resonator; in-situ cell differentiation monitoring; electrical impedance measurement; osteogenic differentiation
TL;DR: A novel technique for the characterization of mammalian cells during cell culture was studied using a lead magnesium niobate-lead titanate single crystal piezoelectric resonator to observe changes in material properties of human adipose derived stem cells during both proliferation and osteogenic differentiation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Cell signaling in tenocytes: Response to load and ligands in health and disease

Metabolic Influences on Risk for Tendon Disorders, 920, 79–95.

By: M. Wall, N. Dyment, J. Bodle, J. Volmer, E. Loboa, A. Cederlund, A. Fox, A. Banes

Source: NC State University Libraries
Added: August 6, 2018

2016 review

Concise Review: Primary Cilia: Control Centers for Stem Cell Lineage Specification and Potential Targets for Cell-Based Therapies

[Review of ]. STEM CELLS, 34(6), 1445–1454.

By: J. Bodle n & E. Loboa n

author keywords: Primary cilia; Tissue regeneration; Adipose stem cells; Bone marrow stromal cells; Cell biology; Stem cell-microenvironment interactions; Cellular therapy; Progenitor cells
MeSH headings : Animals; Cell Lineage; Cilia / metabolism; Disease Models, Animal; Humans; Mechanotransduction, Cellular; Stem Cell Transplantation; Stem Cells / cytology; Stem Cells / metabolism
TL;DR: The goal of this review is to highlight the cilium's function and integrate this knowledge into the working knowledge of stem cell biologists and tissue engineers developing regenerative medicine technologies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Creating tissues from textiles: scalable nonwoven manufacturing techniques for fabrication of tissue engineering scaffolds

BIOMEDICAL MATERIALS, 11(1).

By: S. Tuin n, B. Pourdeyhimi n  & E. Loboa n

author keywords: human adipose derived stem cells; nonwoven tissue engineering scaffolds; scalable tissue engineering
MeSH headings : Adipogenesis / physiology; Biocompatible Materials / chemical synthesis; Cell Differentiation / physiology; Cell Survival / physiology; Cells, Cultured; Equipment Design; Equipment Failure Analysis; Humans; Lactic Acid / chemistry; Materials Testing; Osteogenesis / physiology; Polyesters; Polymers / chemistry; Stem Cells / cytology; Stem Cells / physiology; Textiles; Tissue Engineering / instrumentation; Tissue Engineering / methods; Tissue Scaffolds
TL;DR: Nonwovens manufactured via these industry standard, commercially relevant manufacturing techniques were capable of supporting hASC attachment, proliferation, and both adipogenic and osteogenic differentiation of hASC, making them promising candidates for commercialization and translation of nonwoven scaffold based tissue engineering strategies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Electrospun nanofibrous scaffolds increase the efficacy of stem cell-mediated therapy of surgically resected glioblastoma

BIOMATERIALS, 90, 116–125.

By: J. Bago *, G. Pegna*, O. Okolie*, M. Mohiti-Asli n, E. Loboa n & S. Hingtgen *

author keywords: Cytotoxic stem cell; Electrospun nanofibrous scaffolds; Surgical resection; Cancer; Molecular imaging
MeSH headings : Animals; Antineoplastic Agents / administration & dosage; Antineoplastic Agents / therapeutic use; Brain / pathology; Brain / surgery; Brain Neoplasms / pathology; Brain Neoplasms / surgery; Brain Neoplasms / therapy; Cell Line; Cell Line, Tumor; Drug Delivery Systems / methods; Glioblastoma / pathology; Glioblastoma / surgery; Glioblastoma / therapy; Humans; Mice, Nude; Nanofibers / chemistry; Nanofibers / ultrastructure; Polyesters / chemistry; Stem Cell Transplantation / methods; Stem Cells / cytology; Tissue Scaffolds / chemistry
TL;DR: A biocompatible electrospun nanofibrous scaffold (bENS) implant capable of delivering and retaining tumor-homing cytotoxic stem cells that suppress recurrence of post-surgical GBM is described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Evaluation of Silver Ion-Releasing Scaffolds in a 3D Coculture System of MRSA and Human Adipose-Derived Stem Cells for Their Potential Use in Treatment or Prevention of Osteomyelitis

TISSUE ENGINEERING PART A, 22(21-22), 1258–1263.

By: M. Mohiti-Asli n, C. Molina n, T. Diteepeng *, B. Pourdeyhimi n  & E. Loboa n

author keywords: nanofiber; antimicrobial; coculture; osteomyelitis
MeSH headings : Adipose Tissue / metabolism; Cell Differentiation / drug effects; Coculture Techniques; Humans; Methicillin-Resistant Staphylococcus aureus / growth & development; Osteogenesis / drug effects; Osteomyelitis / prevention & control; Silver / chemistry; Silver / pharmacokinetics; Silver / pharmacology; Stem Cells / metabolism; Tissue Scaffolds / chemistry
TL;DR: It is suggested that these biocompatible, degradable, silver ion-releasing scaffolds can be used at an infection site to treat osteomyelitis and/or to coat bone implants as a preventative measure against infection postsurgery. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Fabrication of novel high surface area mushroom gilled fibers and their effects on human adipose derived stem cells under pulsatile fluid flow for tissue engineering. applications

ACTA BIOMATERIALIA, 36, 220–230.

By: S. Tuin n, B. Pourdeyhimi n  & E. Loboa n

author keywords: Gilled high surface area fibers; Human adipose derived stem cells; Pulsatile fluid slow; Shear stress; Scalable tissue engineering scaffolds
MeSH headings : Adipose Tissue / cytology; Adipose Tissue / metabolism; Agaricales; Cells, Cultured; Humans; Pulsatile Flow; Stem Cells / cytology; Stem Cells / metabolism; Tissue Engineering / instrumentation; Tissue Engineering / methods; Tissue Scaffolds / chemistry
TL;DR: The successful fabrication of novel high surface area gilled fiber scaffolds for tissue engineering applications is reported for the first time, and in the absence of osteogenic induction factors, gilled fibers led to significantly increased mRNA expression of an early marker for osteogenesis after exposure to pulsatile fluid flow. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Facilitating Teamwork in Adolescent and Young Adult Oncology

JOURNAL OF ONCOLOGY PRACTICE, 12(11), 1067-+.

By: R. Johnson*, C. Macpherson*, A. Smith *, R. Block * & J. Keyton *

MeSH headings : Adolescent; Adult; Communication; Cooperative Behavior; Humans; Interprofessional Relations; Male; Patient Care Team / organization & administration; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / drug therapy; Trust; Young Adult
TL;DR: A case of a young adult patient in the days immediately after a cancer diagnosis illustrates the critical importance of three interrelated core coordinating mechanisms-closed-loop communication, shared mental models, and mutual trust-of teamwork in an adolescent and young adult multidisciplinary oncology team. (via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Mechanical and Vascular Cues Synergistically Enhance Osteogenesis in Human Mesenchymal Stem Cells

TISSUE ENGINEERING PART A, 22(15-16), 997–1005.

By: A. Steward  n, J. Cole n , F. Ligler n & E. Loboa n

Contributors: A. Steward  n, J. Cole n , F. Ligler n & E. Loboa n

MeSH headings : Adolescent; Adult; Antigens, Differentiation / biosynthesis; Coculture Techniques; Endothelial Cells / cytology; Endothelial Cells / metabolism; Female; Humans; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Neovascularization, Physiologic; Osteogenesis
TL;DR: The concurrent application of strain to an MSC-EC co- culture resulted in a significant increase in calcium accretion and mineral deposition, suggesting that co-culture and strain synergistically enhance osteogenesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Continuous-wave stimulated emission depletion microscope for imaging actin cytoskeleton in fixed and live cells

Sensors (Basel, Switzerland), 15(9), 24178–24190.

By: B. Neupane, T. Jin, L. Mellor, E. Loboa, F. Ligler & G. Wang

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Extracellular Calcium Modulates Chondrogenic and Osteogenic Differentiation of Human Adipose-Derived Stem Cells: A Novel Approach for Osteochondral Tissue Engineering Using a Single Stem Cell Source

TISSUE ENGINEERING PART A, 21(17-18), 2323–2333.

By: L. Mellor n, M. Mohiti-Asli n, J. Williams n, A. Kannan n, M. Dent n, F. Guilak *, E. Loboa n

MeSH headings : Adipose Tissue / cytology; Adult; Calcification, Physiologic / drug effects; Calcium / pharmacology; Calcium Phosphates / pharmacology; Cell Differentiation / drug effects; Cells, Cultured; Chondrogenesis / drug effects; Extracellular Space / chemistry; Gene Expression Regulation / drug effects; Glycosaminoglycans / metabolism; Humans; Immunohistochemistry; Lactic Acid / pharmacology; Osteogenesis / drug effects; Polyesters; Polymers / pharmacology; Stem Cells / cytology; Stem Cells / drug effects; Stem Cells / ultrastructure; Tissue Engineering / methods; Tissue Scaffolds; Young Adult
TL;DR: This is the first study to report the effects of elevated calcium on chondrogenic differentiation of hASC, and to develop osteochondral nanofibrous scaffolds using a single cell source and controlled calcium release to induce site-specific differentiation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 review

Naturally derived and synthetic scaffolds for skeletal muscle reconstruction

[Review of ]. ADVANCED DRUG DELIVERY REVIEWS, 84, 208–221.

By: M. Wolf *, C. Dearth *, S. Sonnenberg n, E. Loboa n & S. Badylak *

author keywords: Extracellular matrix (ECM); Electrospinning; Hybrid materials; Hydrogel; Mesh; Volumetric muscle loss (VML)
MeSH headings : Biocompatible Materials / administration & dosage; Humans; Muscle, Skeletal / physiology; Polymers / administration & dosage; Regeneration / physiology; Tissue Engineering / methods; Tissue Scaffolds
TL;DR: Advances in scaffolds using muscle tissue engineering are reviewed and next generation naturally derived/synthetic "hybrid materials" would combine the advantageous properties of these materials to create an optimal platform for cell/drug delivery and possess inherent bioactive properties. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Our Fat Future: Translating Adipose Stem Cell Therapy

STEM CELLS TRANSLATIONAL MEDICINE, 4(9), 974–979.

By: R. Nordberg n & E. Loboa n

MeSH headings : Adipocytes / cytology; Adipocytes / physiology; Adipose Tissue / cytology; Adipose Tissue / physiology; Automation, Laboratory / instrumentation; Biomimetic Materials / chemistry; Biosensing Techniques / instrumentation; Cell Differentiation; Cell- and Tissue-Based Therapy / methods; Clinical Trials as Topic; Humans; Stem Cell Transplantation / methods; Stem Cells / cytology; Stem Cells / physiology; Translational Research, Biomedical / legislation & jurisprudence; Translational Research, Biomedical / trends; United States; United States Food and Drug Administration / legislation & jurisprudence; Workforce
TL;DR: The progress and challenges of bringing adipose stem cell therapy into mainstream clinical use are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Translating textiles to tissue engineering: Creation and evaluation of microporous, biocompatible, degradable scaffolds using industry relevant manufacturing approaches and human adipose derived stem cells

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 103(5), 1050–1058.

By: C. Haslauer n, M. Avery n, B. Pourdeyhimi n  & E. Loboa n

author keywords: porous scaffolds; bone tissue engineering; Islands-In-The-Sea; human adipose-derived stem cells
MeSH headings : Absorbable Implants; Adipose Tissue / cytology; Adipose Tissue / metabolism; Cell Differentiation; Cells, Cultured; Humans; Materials Testing; Osteogenesis; Porosity; Stem Cells / cytology; Stem Cells / metabolism; Textiles; Tissue Engineering; Tissue Scaffolds / chemistry
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Age-Related Effects on the Potency of Human Adipose-Derived Stem Cells: Creation and Evaluation of Superlots and Implications for Musculoskeletal Tissue Engineering Applications

Tissue Engineering Part C: Methods, 20(12), 972–983.

By: J. Bodle n, S. Teeter n , B. Hluck n, J. Hardin n, S. Bernacki n & E. Loboa n

MeSH headings : Adipogenesis; Adipose Tissue / cytology; Adult; Aged; Aged, 80 and over; Aging / physiology; Cell Line; Cell Proliferation; Cell Separation; DNA / metabolism; Female; Humans; Middle Aged; Musculoskeletal System / metabolism; Osteogenesis; Postmenopause; Premenopause; Proteins / metabolism; Reproducibility of Results; Stem Cells / cytology; Tissue Engineering / methods; Young Adult
TL;DR: In general, superlots effectively represented the average differentiation behavior of each of their contributing cell populations and could provide a powerful tool for increasing experimental throughput to more efficiently utilize resources when studying hASC differentiation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2014 journal article

Cyclic Tensile Strain Enhances Osteogenesis and Angiogenesis in Mesenchymal Stem Cells from Osteoporotic Donors

TISSUE ENGINEERING PART A, 20(1-2), 67–78.

By: A. Charoenpanich n, M. Wall n, C. Tucker *, D. Andrews*, D. Lalush n, D. Dirschl *, E. Loboa n

MeSH headings : Aged, 80 and over; Calcium / metabolism; Cell Differentiation; Cell Proliferation; Cell Separation; Female; Gene Expression Regulation; Gene Regulatory Networks; Humans; Mesenchymal Stem Cells / metabolism; Mesenchymal Stem Cells / pathology; Neovascularization, Physiologic / genetics; Oligonucleotide Array Sequence Analysis; Osteogenesis / genetics; Osteoporosis, Postmenopausal / genetics; Osteoporosis, Postmenopausal / pathology; Proto-Oncogene Mas; Real-Time Polymerase Chain Reaction; Signal Transduction / genetics; Stress, Mechanical; Tensile Strength; Tissue Donors; White People
TL;DR: It is demonstrated that hMSC from aged, osteoporotic donors are capable of enhanced osteogenic differentiation in response to 10% cyclic tensile strain with significant increases in the expression of genes associated with enhanced cell proliferation, musculoskeletal development, and angiogenesis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2014 review

Interconnected, microporous hollow fibers for tissue engineering: Commercially relevant, industry standard scale-up manufacturing

[Review of ]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 102(9), 3311–3323.

By: S. Tuin n, B. Pourdeyhimi n  & E. Loboa n

author keywords: spunbond process; hollow porous fibers; industry standard scale-up manufacturing; tissue engineering; stem cells
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Novel, silver-ion-releasing nanofibrous scaffolds exhibit excellent antibacterial efficacy without the use of silver nanoparticles

ACTA BIOMATERIALIA, 10(5), 2096–2104.

By: M. Mohiti-Asli n, B. Pourdeyhimi n  & E. Loboa n

author keywords: Antimicrobial; Silver; Biocompatibility; Wound dressing; Nanofibers
MeSH headings : Adult; Anti-Bacterial Agents / pharmacology; Biocompatible Materials / pharmacology; Cell Proliferation / drug effects; Cell Shape / drug effects; Cell Survival / drug effects; Dermis / cytology; Epidermal Cells; Escherichia coli / drug effects; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / ultrastructure; Humans; Ions; Keratinocytes / cytology; Keratinocytes / drug effects; Keratinocytes / ultrastructure; Lactic Acid / chemistry; Mass Spectrometry; Metal Nanoparticles / chemistry; Microbial Sensitivity Tests; Nanofibers / chemistry; Nanofibers / ultrastructure; Photoelectron Spectroscopy; Polyesters; Polymers / chemistry; Silver / pharmacology; Staphylococcus aureus / drug effects; Tissue Scaffolds / chemistry
TL;DR: This is the first study to show that silver ions can be effectively delivered with nanofibrous scaffolds without the use of silver nanoparticles, and a new silver ion release polymeric coating is presented and evaluated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Primary cilium mechanotransduction of tensile strain in 3D culture: Finite element analyses of strain amplification caused by tensile strain applied to a primary cilium embedded in a collagen matrix

JOURNAL OF BIOMECHANICS, 47(9), 2211–2217.

By: P. Mathieu n, J. Bodle n & E. Loboa n

author keywords: Primary cilia; Finite element analysis; Mechanotransduction; Tensile strain; Ciliary membrane; Mechanobiology; Ciliary pocket; Adipose derived stem cells
MeSH headings : Adipose Tissue / cytology; Cells, Cultured; Cilia / physiology; Collagen; Finite Element Analysis; Humans; Mechanotransduction, Cellular; Models, Biological; Osteogenesis / physiology; Stem Cells / cytology; Stress, Mechanical
TL;DR: This is the first study to model the effects of tensile strain on the primary cilium and provides newfound insight into the potential role of thePrimary cilia as a mechanosensor, particularly in Tensile strain and potentially a multitude of other mechanical stimuli beyond fluid shear. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Skin Tissue Engineering for the Infected Wound Site: Biodegradable PLA Nanofibers and a Novel Approach for Silver Ion Release Evaluated in a 3D Coculture System of Keratinocytes and Staphylococcus aureus

TISSUE ENGINEERING PART C-METHODS, 20(10), 790–797.

By: M. Mohiti-Asli n, B. Pourdeyhimi n  & E. Loboa n

MeSH headings : Biocompatible Materials / chemistry; Cell Proliferation / drug effects; Cell Survival / drug effects; Coculture Techniques; DNA / metabolism; Epidermal Cells; Humans; Ions; Keratinocytes / cytology; Keratinocytes / drug effects; Keratinocytes / ultrastructure; Lactic Acid / chemistry; Nanofibers / chemistry; Nanofibers / ultrastructure; Polyesters; Polymers / chemistry; Porosity; Silver / analysis; Skin / pathology; Staphylococcus aureus / cytology; Staphylococcus aureus / drug effects; Staphylococcus aureus / growth & development; Tissue Engineering / methods; Tissue Scaffolds / chemistry; Wound Infection / microbiology; Wound Infection / pathology
TL;DR: It is found that highly porous AgMPs could be successfully incorporated in nanofibrous wound dressings, and exhibited comparable antimicrobial efficacy and cytotoxicity to AgNPs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Alternating Current Electric Fields of Varying Frequencies: Effects on Proliferation and Differentiation of Porcine Neural Progenitor Cells

Cellular Reprogramming, 15(5), 405–412.

By: J. Lim n, S. McCullen n, J. Piedrahita n, E. Loboa n & N. Olby n 

MeSH headings : Animals; Cell Differentiation; Cell Proliferation; Cells, Cultured; Electricity; Electrodes; Fluorescence; Glial Fibrillary Acidic Protein / metabolism; Neural Stem Cells / cytology; Swine; Swine, Miniature; Tubulin / metabolism
TL;DR: Higher frequency stimulation of low-frequency alternating current EFs via IDEs did not alter the alignment and proliferation of porcine neural progenitor cells, but higher frequency stimulation appeared to delay differentiation into mature astrocytes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID
Added: August 6, 2018

2013 journal article

In vitro testicular toxicity models: Opportunities for advancement via biomedical engineering techniques

Altex-Alternatives to Animal Experimentation, 30(3), 353–377.

By: L. Saldutti, B. Beyer, W. Breslin, T. Brown, R. Chapin, S. Campion, B. Enright, E. Faustman ...

Source: NC State University Libraries
Added: August 6, 2018

2013 journal article

Primary Cilia: The Chemical Antenna Regulating Human Adipose-Derived Stem Cell Osteogenesis

PLOS ONE, 8(5).

By: J. Bodle n, C. Rubenstein n, M. Phillips  n, S. Bernacki n, J. Qi *, A. Banes n, E. Loboa n

MeSH headings : Adipose Tissue / cytology; Analysis of Variance; Cell Differentiation / physiology; Cilia / physiology; Cilia / ultrastructure; Gene Knockdown Techniques; Genetic Engineering / methods; Humans; Microscopy, Fluorescence; Multipotent Stem Cells / physiology; Osteogenesis / physiology; RNA Interference; RNA, Small Interfering / genetics; Real-Time Polymerase Chain Reaction; TRPP Cation Channels / genetics; TRPP Cation Channels / metabolism; Tumor Suppressor Proteins / genetics; Tumor Suppressor Proteins / metabolism
TL;DR: The results indicate that the structure of the primary cilium is intimately associated with the process of hASC osteogenic differentiation and that its associated proteins are critical players in this process. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Sensitization of Transforming Growth Factor-beta Signaling by Multiple Peptides Patterned on DNA Nanostructures

BIOMACROMOLECULES, 14(12), 4157–4160.

By: R. Pedersen*, E. Loboa n & T. LaBean n

MeSH headings : Animals; Biotin / chemistry; Cell Line; DNA / chemistry; Drug Carriers / chemistry; Drug Carriers / pharmacology; Genes, Reporter; Mice; Nanostructures / chemistry; Nanostructures / ultrastructure; Peptide Fragments / chemistry; Peptide Fragments / pharmacology; Receptors, Transforming Growth Factor beta / metabolism; Signal Transduction; Streptavidin / chemistry; Transforming Growth Factor beta / pharmacology; Transforming Growth Factor beta / physiology
TL;DR: This work reports sensitization of a cellular signaling pathway by addition of functionalized DNA nanostructures, an important initial step toward realization of DNA as a self-assembling and biologically compatible material for use in tissue engineering and drug delivery. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

The Effects of Cyclic Hydrostatic Pressure on Chondrogenesis and Viability of Human Adipose- and Bone Marrow-Derived Mesenchymal Stem Cells in Three-Dimensional Agarose Constructs

TISSUE ENGINEERING PART A, 19(1-2), 299–306.

By: J. Puetzer  n, J. Williams n, A. Gillies n, S. Bernacki n & E. Loboa n

MeSH headings : Adipocytes / cytology; Adipocytes / physiology; Cell Differentiation; Cell Proliferation; Cell Survival; Cells, Cultured; Chondrogenesis / physiology; Equipment Design; Humans; Hydrostatic Pressure; Mechanotransduction, Cellular / physiology; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / physiology; Sepharose / chemistry; Tissue Scaffolds
TL;DR: It is suggested that a 2% agarose hydrogel may not optimally support hASC or hMSC viability in a complete growth medium in the absence of soluble chondrogenic inducing factors over long culture durations. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Cytoskeletal and Focal Adhesion Influences on Mesenchymal Stem Cell Shape, Mechanical Properties, and Differentiation Down Osteogenic, Adipogenic, and Chondrogenic Pathways

TISSUE ENGINEERING PART B-REVIEWS, 18(6), 436–444.

By: P. Mathieu n & E. Loboa n

MeSH headings : Adipogenesis / physiology; Animals; Cell Differentiation; Cell Shape; Chondrogenesis / physiology; Cytoskeleton; Focal Adhesions; Humans; Mechanotransduction, Cellular; Mesenchymal Stem Cells / cytology; Osteogenesis / physiology
TL;DR: This review analyzes the significant contribution of actin and integrin distribution, and the smaller role of microtubules, in regulating MSC fate and shows that mechanical stimuli can control MSC lineage specification. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Release Profiles of Tricalcium Phosphate Nanoparticles from Poly(L-lactic acid) Electrospun Scaffolds with Single Component, Core-Sheath, or Porous Fiber Morphologies: Effects on hASC Viability and Osteogenic Differentiation

MACROMOLECULAR BIOSCIENCE, 12(7), 893–900.

By: M. Asli n, B. Pourdeyhimi n  & E. Loboa n

author keywords: biomaterials; controlled release scaffolds; human adipose derived stem cells; nanoparticles; osteogenesis
MeSH headings : Adipose Tissue / cytology; Adipose Tissue / drug effects; Adult; Biocompatible Materials / chemical synthesis; Biocompatible Materials / pharmacology; Bone and Bones / cytology; Bone and Bones / physiology; Calcium Phosphates / chemistry; Cell Differentiation / drug effects; Cell Proliferation / drug effects; Cell Survival / drug effects; Cells, Cultured; Female; Humans; Kinetics; Lactic Acid / chemistry; Microscopy, Electron, Scanning; Nanoparticles / chemistry; Nanoparticles / ultrastructure; Polyesters; Polymers / chemistry; Porosity; Stem Cells / cytology; Stem Cells / drug effects; Surface Properties; Tissue Engineering; Tissue Scaffolds
TL;DR: Results indicate that single component and porous fiber scaffolds exhibit an initial-burst release profile whereas core-sheath fibers show a steady release, and osteogenic differentiation of hASC and cell-mediated calcium accretion are largest on porous fibers. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

Silver nanoparticles do not influence stem cell differentiation but cause minimal toxicity

NANOMEDICINE, 7(8), 1197–1209.

By: M. Samberg n, E. Loboa n, S. Oldenburg* & N. Monteiro-Riviere n 

Contributors: M. Samberg n, E. Loboa n, S. Oldenburg* & N. Monteiro-Riviere n 

author keywords: in vitro; nanoparticles; nanotechnology; nanotoxicology; scaffolds; silver; stem cells
MeSH headings : Adipose Tissue / cytology; Adult; Cell Differentiation / drug effects; Cell Survival / drug effects; Cells, Cultured; Female; Humans; Metal Nanoparticles / toxicity; Metal Nanoparticles / ultrastructure; Silver / toxicity; Stem Cells / cytology; Stem Cells / drug effects; Young Adult
TL;DR: The biocompatibility of Ag-NPs with both undifferentiated and differentiated hASC establishes their suitability for incorporation into tissue-engineered graft scaffolds, for the prevention of bacterial contamination upon implantation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Adipose-Derived Stem Cells in Functional Bone Tissue Engineering: Lessons from Bone Mechanobiology

TISSUE ENGINEERING PART B-REVIEWS, 17(3), 195–211.

By: J. Bodle n, A. Hanson n & E. Loboa n

MeSH headings : Adipose Tissue / cytology; Adipose Tissue / physiology; Adipose Tissue / transplantation; Animals; Humans; Mechanotransduction, Cellular / physiology; Osteogenesis / physiology; Stem Cell Transplantation / trends; Stem Cells / cytology; Stem Cells / physiology; Tissue Engineering / trends
TL;DR: The current knowledge base in bone mechanobiology is outlined to investigate how the knowledge from this area has been applied to the various stem cell-based approaches to engineering bone tissue constructs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Collagen-PCL Sheath-Core Bicomponent Electrospun Scaffolds Increase Osteogenic Differentiation and Calcium Accretion of Human Adipose-Derived Stem Cells

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 22(13), 1695–1712.

By: C. Haslauer n, A. Moghe n, J. Osborne n, B. Gupta n & E. Loboa n

author keywords: Biomedical applications; tissue engineering; fibers; nanostructures; human adipose-derived stem cells; electrospinning
MeSH headings : Adipose Tissue / cytology; Adipose Tissue / metabolism; Bone and Bones; Calcium / metabolism; Cell Differentiation; Cell Proliferation; Collagen / chemistry; Humans; Microscopy, Electron, Scanning; Nanofibers / chemistry; Osteogenesis; Polyesters / chemistry; Polymers; Stem Cells / cytology; Stem Cells / metabolism; Tissue Engineering; Tissue Scaffolds
TL;DR: The sheath-core composite fibers significantly increased calcium accretion of hASCs, indicating that collagen–PCL sheath–core bicomponent structures have potential for bone tissue engineering applications using h ASCs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Dietary Calcium Restriction Affects Mesenchymal Stem Cell Activity and Bone Development in Neonatal Pigs

JOURNAL OF NUTRITION, 141(3), 373–379.

By: A. Mahajan n, L. Alexander n, B. Seabolt n, D. Catrambone*, J. McClung*, J. Odle n , T. Pfeiler n, E. Loboa n, C. Stahl n

Contributors: A. Mahajan n, L. Alexander n, B. Seabolt n, D. Catrambone*, J. McClung*, J. Odle n , T. Pfeiler n, E. Loboa n, C. Stahl n

MeSH headings : 25-Hydroxyvitamin D3 1-alpha-Hydroxylase / genetics; 25-Hydroxyvitamin D3 1-alpha-Hydroxylase / metabolism; Animals; Animals, Newborn; Bone Density; Bone Development; Bone and Bones / chemistry; Calcitriol / blood; Calcium / blood; Calcium / deficiency; Calcium, Dietary / administration & dosage; Cell Differentiation; Cell Proliferation; Cells, Cultured; Female; Gene Expression Regulation; Male; Mechanical Phenomena; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Nutritional Status; Parathyroid Hormone / blood; RNA, Messenger / metabolism; Receptors, Calcium-Sensing / genetics; Receptors, Calcium-Sensing / metabolism; Sus scrofa
TL;DR: The results indicate that neonatal Ca nutrition is crucial for bone integrity and suggest that early-life Ca restriction may have long-term effects on bone integrity via programming of MSC. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
5. Gender Equality (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

Laser Ablation Imparts Controlled Micro-Scale Pores in Electrospun Scaffolds for Tissue Engineering Applications

Annals of Biomedical Engineering, 39(12), 3021–3030.

By: S. McCullen n, S. Gittard n, P. Miller n, B. Pourdeyhimi n , R. Narayan n & E. Loboa n

author keywords: Human adipose-derived stem cells; Laser ablation; Electrospun; Scaffold; Micro-machining
MeSH headings : Adipocytes; Cell Adhesion; Cell Survival; Cells, Cultured; Female; Humans; Lactic Acid / chemistry; Laser Therapy; Mesenchymal Stem Cells; Middle Aged; Polyesters; Polymers / chemistry; Tissue Engineering / methods; Tissue Scaffolds
TL;DR: The process of laser ablation could impart many beneficial features to electrospun scaffolds by increasing mass transport and mimicking micro-scale features and assisting in patterning of cells around micro-machined features. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, Crossref
Added: August 6, 2018

2011 journal article

Microarray Analysis of Human Adipose-Derived Stem Cells in Three-Dimensional Collagen Culture: Osteogenesis Inhibits Bone Morphogenic Protein and Wnt Signaling Pathways, and Cyclic Tensile Strain Causes Upregulation of Proinflammatory Cytokine Regulators and Angiogenic Factors

TISSUE ENGINEERING PART A, 17(21-22), 2615–2627.

By: A. Charoenpanich n, M. Wall n, C. Tucker *, D. Andrews*, D. Lalush n & E. Loboa n

MeSH headings : Adipose Tissue / cytology; Adult; Angiogenesis Inducing Agents / metabolism; Bone Morphogenetic Proteins / genetics; Bone Morphogenetic Proteins / metabolism; Cells, Cultured; Collagen / metabolism; Cytokines / genetics; Cytokines / metabolism; Humans; Insulin-Like Growth Factor I / genetics; Middle Aged; Oligonucleotide Array Sequence Analysis / methods; Osteogenesis / genetics; Osteogenesis / physiology; Platelet-Derived Growth Factor / genetics; Signal Transduction / genetics; Signal Transduction / physiology; Stem Cells / cytology; Stem Cells / metabolism; Wnt Signaling Pathway / genetics; Wnt Signaling Pathway / physiology
TL;DR: This first study to investigate the effects of both 3D culture and mechanical load on hASC osteogenic differentiation and response to mechanical stimuli identified specific genes and pathways associated with mechanical response and osteogenic potential of hASC, thus providing significant information toward improved understanding of its use for functional bone tissue engineering applications. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Application of Low-Frequency Alternating Current Electric Fields Via Interdigitated Electrodes: Effects on Cellular Viability, Cytoplasmic Calcium, and Osteogenic Differentiation of Human Adipose-Derived Stem Cells

TISSUE ENGINEERING PART C-METHODS, 16(6), 1377–1386.

By: S. McCullen n, J. McQuilling n, R. Grossfeld n, J. Lubischer n , L. Clarke n  & E. Loboa n

MeSH headings : Adipose Tissue / cytology; Adult Stem Cells / cytology; Adult Stem Cells / metabolism; Adult Stem Cells / physiology; Calcium / metabolism; Cell Differentiation / physiology; Cell Proliferation; Cell Survival / physiology; Cells, Cultured; Cytoplasm / metabolism; Electric Stimulation / methods; Electricity; Electrodes; Humans; Osteoblasts / metabolism; Osteoblasts / physiology; Osteogenesis / physiology
TL;DR: This is the first study to evaluate the effects of sinusoidal AC electric fields on hASCs and to demonstrate that acute and chronic electric field exposure can significantly increase intracellular calcium signaling and the deposition of accreted calcium under osteogenic stimulation, respectively. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Effect of Varied Ionic Calcium on Human Adipose-Derived Stem Cell Mineralization

TISSUE ENGINEERING PART A, 16(6), 1971–1981.

By: S. McCullen n, J. Zhan*, M. Onorato n, S. Bernacki n & E. Loboa n

MeSH headings : Adipocytes / cytology; Calcium / pharmacology; Cell Differentiation / drug effects; Cell Proliferation / drug effects; Humans; Osteogenesis / drug effects; Spectroscopy, Fourier Transform Infrared; Stem Cells / cytology; Stem Cells / drug effects; Stem Cells / metabolism; Tissue Engineering / methods; X-Ray Diffraction
TL;DR: Ionic calcium should be considered a potent regulator in hASC mineralization and could serve as a potential treatment for inducing prompt ossification of hASC-seeded scaffolds for bone tissue engineering prior to implantation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Fiber-reinforced scaffolds for tissue engineering and regenerative medicine: use of traditional textile substrates to nanofibrous arrays

JOURNAL OF MATERIALS CHEMISTRY, 20(40), 8776–8788.

By: S. McCullen n, C. Haslauer n & E. Loboa n

TL;DR: Fiber reinforcement continues to be an ideal strategy for tissue scaffolds that require mechanical reinforcement while providing high surface volume in a compliant form and next steps to expand this field are discussed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

In Situ Monitoring of Adipogenesis with Human-Adipose-Derived Stem Cells Using Surface-Enhanced Raman Spectroscopy

APPLIED SPECTROSCOPY, 64(11), 1227–1233.

By: B. Moody n, C. Haslauer n, E. Kirk n, A. Kannan n, E. Loboa n & G. McCarty n 

author keywords: Raman spectroscopy; Adipogenesis; Stem cells; Surface-enhanced Raman spectroscopy; SERS
MeSH headings : Adipogenesis / physiology; Adipose Tissue / cytology; Adipose Tissue / metabolism; Adult; Cells, Cultured; Endocytosis; Female; Gold Colloid / chemistry; Gold Colloid / pharmacokinetics; Humans; Metal Nanoparticles / chemistry; Microscopy, Electron, Transmission; Spectrum Analysis, Raman / methods; Stem Cells / cytology; Stem Cells / metabolism
TL;DR: It is shown that intracellular SERS is able to detect the production of lipids as little as one day after the onset of adipogenesis and that a complex interplay between lipids, proteins, and chemical messengers can be observed shortly thereafter. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

In situ collagen polymerization of layered cell-seeded electrospun scaffolds for bone tissue engineering applications

Tissue Engineering. Part C, Methods, 16(5), 1095–1105.

By: S. McCullen, P. Miller, S. Gittard, R. Gorga* , B. Pourdeyhimi* , R. Narayan, E. Loboa*

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2010 journal article

Musculoskeletal mechanobiology: Interpretation by external force and engineered substratum

JOURNAL OF BIOMECHANICS, 43(1), 119–127.

By: S. McCullen n, C. Haslauer n & E. Loboa n

author keywords: Mechanobiology; Musculoskeletal; External forces; Cell-substrate interactions
MeSH headings : Adenosine Triphosphate / metabolism; Animals; Biomechanical Phenomena; Cytoskeleton / physiology; Humans; Mechanotransduction, Cellular; Muscle, Skeletal / physiology; Nitric Oxide / metabolism; Stress, Mechanical; Tensile Strength
TL;DR: This review outlines many of the main mechanotransduction mechanisms known to date, and describes recent literature examining effects of both external forces and cell-substrate interactions on musculoskeletal cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

The Development and Validation of a LIPUS System With Preliminary Observations of Ultrasonic Effects on Human Adult Stem Cells

IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL, 57(9), 1977–1984.

By: S. Marvel*, S. Okrasinski*, S. Bernacki*, E. Loboa* & P. Dayton*

MeSH headings : Adipose Tissue / cytology; Adult; Adult Stem Cells / cytology; Adult Stem Cells / diagnostic imaging; Bone Marrow Cells / cytology; Calcium / analysis; Calcium / metabolism; Cell Growth Processes / physiology; Cells, Cultured; DNA / chemistry; Humans; Reproducibility of Results; Ultrasonics / instrumentation; Ultrasonics / methods; Ultrasonography
TL;DR: The custom LIPUS system designed and validated by exploring the effects of using different pulse repetition frequency (PRF) parameters on human adipose derived adult stem cells and bone marrow derived mesenchymal stem cells was successfully designed to allow extreme parameter variation, specifically PRF, and encourages further studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Composite Tissue Engineering on Polycaprolactone Nanofiber Scaffolds

Annals of Plastic Surgery, 62(5), 505–512.

By: C. Reed*, L. Han *, A. Andrady* , M. Caballero*, M. Jack*, J. Collins *, S. Saba*, E. Loboa n, B. Cairns*, J. van Aalst*

author keywords: nanofiber scaffolds; nanotechnology; composite tissue engineering; keratinocytes; fibroblasts; osteoinduction; fat-derived stem cells; periosteum; periosteal cells
MeSH headings : Adult Stem Cells / cytology; Animals; Biocompatible Materials; Cell Survival; Female; Fibroblasts / cytology; Foreskin / cytology; Humans; Keratinocytes / cytology; Male; Mice; Mice, Inbred C57BL; Nanostructures; Osteogenesis; Periosteum / cytology; Periosteum / growth & development; Polyesters; Plastic Surgery Procedures / methods; Tissue Culture Techniques; Tissue Engineering / methods; Tissue Scaffolds
TL;DR: This work represents initial efforts to create the building blocks for composite tissue reflecting the basic unit of healing, though the major obstacles to the trilaminar construct are maintaining an appropriate interface between the tissue types and neovascularization of the composite structure. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID
Added: August 6, 2018

2009 chapter

Development and application of naturally renewable scaffold materials for bone tissue engineering

In L. A. Lucia & O. J. Rojas (Eds.), Nanotechnology of renewable materials.

By: S. McCullen*, A. Hanson*, L. Lucia*  & E. Loboa*

Ed(s): . L. A. Lucia & O. Rojas

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2009 journal article

Electrospun composite poly(L-lactic acid)/tricalcium phosphate scaffolds induce proliferation and osteogenic differentiation of human adipose-derived stem cells

BIOMEDICAL MATERIALS, 4(3).

By: S. McCullen n, Y. Zhu *, S. Bernacki n, R. Narayan  n, B. Pourdeyhimi n , R. Gorga n , E. Loboa n

MeSH headings : Adipocytes / cytology; Adipocytes / physiology; Biocompatible Materials / chemistry; Calcium Phosphates / chemistry; Cell Culture Techniques / methods; Cell Differentiation; Cell Proliferation; Cells, Cultured; Electrochemistry / methods; Humans; Lactic Acid / chemistry; Materials Testing; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / physiology; Osteoblasts / cytology; Osteoblasts / physiology; Osteogenesis / physiology; Polyesters; Polymers / chemistry; Rotation; Tissue Engineering / methods
TL;DR: It was noted that at the highest TCP loading levels of 10 and 20 wt%, there was a dramatic increase in the amount of cell-mediated mineralization compared to the 5 wt% TCP and the neat PLA scaffold, suggesting that local environment cues provided by the biochemical nature of the scaffold can accelerate the overall osteogenic differentiation of hASCs and encourage rapid ossification. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Expression of Proinflammatory Cytokines by Human Mesenchymal Stem Cells in Response to Cyclic Tensile Strain

JOURNAL OF CELLULAR PHYSIOLOGY, 219(1), 77–83.

By: R. Sumanasinghe n, T. Pfeiler n, N. Monteiro-Riviere n  & E. Loboa n

Contributors: R. Sumanasinghe n, T. Pfeiler n, N. Monteiro-Riviere n  & E. Loboa n

MeSH headings : Animals; Bone Marrow Cells / cytology; Bone Marrow Cells / physiology; Cells, Cultured; Cytokines / metabolism; Humans; Interleukin-1beta / metabolism; Interleukin-6 / metabolism; Interleukin-8 / metabolism; Male; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; Stress, Mechanical; Stress, Physiological; Tensile Strength; Tumor Necrosis Factor-alpha / metabolism; Young Adult
TL;DR: IL‐6 and IL‐8 expressions by hMSCs were induced by cyclic tensile strain and osteogenic differentiating media, indicating that IL‐ 6 andIL‐8 may be functioning as autocrine signals during osteogenic differentiation of h MSCs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

Mesenchymal stem cell-seeded collagen matrices for bone repair: Effects of cyclic tensile strain, cell density, and media conditions on matrix contraction in vitro

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 88A(3), 778–786.

By: R. Sumanasinghe n, J. Osborne n & E. Loboa n

author keywords: bone tissue engineering; mechanical stimulation; osteogenic differentiation; collagen contraction; mesenchymal stem cells
MeSH headings : Adult; Bone and Bones / cytology; Cell Culture Techniques / methods; Cell Differentiation; Cell Survival; Collagen; Culture Media, Conditioned; Humans; Male; Mesenchymal Stem Cells / cytology; Tensile Strength
TL;DR: Findings suggest a correlation between hMSC proliferation and osteogenic differentiation on collagen matrix contraction that is affected by media, cell-seeding density, and cyclic tensile strain. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Osteogenic Effects of Rest Inserted and Continuous Cyclic Tensile Strain on hASC Lines with Disparate Osteodifferentiation Capabilities

ANNALS OF BIOMEDICAL ENGINEERING, 37(5), 955–965.

By: A. Hanson n, S. Marvel n, S. Bernacki n, A. Banes n, J. Aalst* & E. Loboa n

author keywords: Adipose derived stem cells; Mesenchymal stem cells; Osteogenesis; Mechanical effects on cells and tissues; Tissue engineering
MeSH headings : Adipose Tissue / cytology; Adult Stem Cells; Calcium / metabolism; Cell Differentiation; Cell Proliferation; Cells, Cultured; Humans; Mesenchymal Stem Cells; Models, Biological; Osteogenesis / physiology; Stress, Mechanical; Tensile Strength; Tissue Engineering / methods
TL;DR: Results showed that both continuous and rest inserted cyclic tensile strain increased the amount and rate of calcium deposition for both high and low calcium depositing hASC lines as compared to unstrained controls, suggesting that mechanical loading has a greater effect on cell lines that already have an innate ability to produce bone. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Cellular Incorporation Into Electrospun Nanofibers

Annals of Plastic Surgery, 60(5), 577–583.

By: J. van Aalst, C. Reed, L. Han, T. Andrady, M. Hromadka, S. Bernacki*, K. Kolappa, J. Collins, E. Loboa*

author keywords: nanofibers; fibroblasts; adipose-derived adult stem cells; electrospinning; nanotechnology
MeSH headings : Cell Proliferation; Cell Survival; Fibroblasts / physiology; Humans; Nanostructures / chemistry; Polymers; Tissue Engineering; Tissue Scaffolds
TL;DR: Both fibroblasts and hADAS survived the electrospinning process and were incorporated into PVA nanofibers, showing retained ability to undergo chondrogenesis and maintained viability, proliferation, and function. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref, ORCID
Added: August 6, 2018

2008 journal article

Cyclic tensile strain increases interactions between human epidermal keratinocytes and quantum dot nanoparticles

TOXICOLOGY IN VITRO, 22(2), 491–497.

By: J. Rouse n, C. Haslauer n, E. Loboa n & N. Monteiro-Riviere n 

Contributors: J. Rouse n, C. Haslauer n, E. Loboa n & N. Monteiro-Riviere n 

author keywords: quantum dot nanoparticles; keratinocytes; skin; tensile strain; cellular uptake
MeSH headings : Borates / toxicity; Cell Membrane Permeability / drug effects; Cell Survival / drug effects; Cells, Cultured; Cytokines / analysis; Cytokines / biosynthesis; Epidermal Cells; Epidermis / drug effects; Ethidium; Fluoresceins; Fluorescent Dyes; Humans; Keratinocytes / drug effects; Keratinocytes / physiology; Microscopy, Fluorescence; Pharmaceutical Vehicles / chemistry; Polyethylene Glycols / chemistry; Quantum Dots; Tensile Strength
TL;DR: Application of physiological load conditions can increase cell membrane permeability, thereby increasing the concentration of QD nanoparticles in cells, and addition of strain results in an increase in cytokine production and QD uptake, resulting in irritation and a negative impact on cell viability. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2008 journal article

Finite element modeling of 3D human mesenchymal stem cell-seeded collagen matrices exposed to tensile strain

JOURNAL OF BIOMECHANICS, 41(10), 2289–2296.

By: T. Pfeiler n, R. Sumanasinghe n & E. Loboa n

author keywords: uniaxial tensile strain; HMSCs; collagen; bioreactor; finite element modeling
MeSH headings : Biomechanical Phenomena / methods; Bioreactors; Cell Lineage; Cells, Cultured; Collagen / chemistry; Cytoskeleton / metabolism; Finite Element Analysis; Humans; Mesenchymal Stem Cells / cytology; Models, Theoretical; Pressure; Stress, Mechanical; Tensile Strength; Tissue Engineering / methods
TL;DR: Finite element models were created to evaluate the local loading conditions on bone marrow-derived hMSCs seeded in three-dimensional collagen matrices exposed to cyclic tensile strain and it was determined that globally applied uniaxial tensile strains of 10% and 12% resulted in local strains up to 18.3% and 21.8%, respectively. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 review

Isolation of human mesenchymal stem cells from bone and adipose tissue

[Review of ]. Stem cell culture, 86, 257–278.

By: S. Bernacki, M. Wall & E. Loboa

Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Characterization of electrospun nanocomposite scaffolds and biocompatibility with adipose-derived human mesenchymal stem cells

International Journal of Nanomedicine, 2(2), 253–263.

By: S. McCullen, D. Stevens, W. Roberts, L. Clarke , S. Bernacki, R. Gorga , E. Loboa

Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Differential effects on messenger ribonucleic acid expression by bone marrow-derived human mesenchymal stem cells seeded in agarose constructs due to ramped and steady applications of cyclic hydrostatic pressure

TISSUE ENGINEERING, 13(6), 1151–1158.

By: A. Finger n, C. Sargent n, K. Dulaney n, S. Bernacki n & E. Loboa n

MeSH headings : Adolescent; Adult; Biocompatible Materials / chemistry; Bone Marrow Cells / cytology; Bone Marrow Cells / drug effects; Bone Marrow Cells / metabolism; Cell Culture Techniques / methods; Cells, Cultured; Chondrocytes / cytology; Chondrocytes / drug effects; Chondrocytes / metabolism; Chondrogenesis / drug effects; Chondrogenesis / physiology; Cytoskeletal Proteins / genetics; Cytoskeletal Proteins / metabolism; Gene Expression Regulation, Developmental / drug effects; Gene Expression Regulation, Developmental / physiology; Humans; Male; Mechanotransduction, Cellular / drug effects; Mechanotransduction, Cellular / physiology; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / drug effects; Mesenchymal Stem Cells / metabolism; Pressure; RNA, Messenger / metabolism; Sepharose / chemistry; Tissue Engineering / methods; Transforming Growth Factor beta / administration & dosage
TL;DR: Findings indicate that hydrostatic pressure may induce chondrogenesis in hMSC-seeded agarose constructs without TGF-beta, and that hMSCs are capable of withstanding high initial pressures that may initiate chondrogensesis faster than lower pressures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Effects of oxygen plasma treatment on adipose-derived human mesenchymal stem cell adherence to poly(L-lactic acid) scaffolds

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 18(11), 1387–1400.

By: A. Hanson n, M. Wall n, B. Pourdeyhimi n  & E. Loboa n

author keywords: hydrophilicity; non-woven PLLA scaffold; cell morphology; cell viability; melt-blown PLLA scaffold
MeSH headings : Adipocytes / cytology; Cell Adhesion / drug effects; Cell Survival; Cells, Cultured; Humans; Lactic Acid / chemistry; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / drug effects; Microscopy, Electron, Scanning; Oxygen / chemistry; Oxygen / pharmacology; Polyesters; Polymers / chemistry; Tissue Scaffolds / chemistry
TL;DR: Enhanced cell adhesion to non-woven poly(L-lactic acid) (PLLA) scaffolds using oxygen plasma treatment to increase surface hydroxyl groups and thereby enhance substrate hydrophilicity was enhanced. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2007 journal article

Effects of serial passaging on the adipogenic and osteogenic differentiation potential of adipose-derived human mesenchymal stem cells

TISSUE ENGINEERING, 13(6), 1291–1298.

By: M. Wall n, S. Bernacki n & E. Loboa n

MeSH headings : Adipocytes / cytology; Adipocytes / physiology; Adipogenesis / physiology; Adult; Cell Culture Techniques / methods; Cell Differentiation; Cells, Cultured; Female; Humans; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / physiology; Middle Aged; Osteocytes / cytology; Osteocytes / physiology; Osteogenesis / physiology; Tissue Engineering / methods
TL;DR: The results of this study indicate that adipose-derived hMSCs are capable of both adipogenic and osteogenic differentiation through 10 passages (34 population doublings) but that osteogenic differentiate may start to dominate at later passages. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Genetic background influences fluoride's effects on osteoclastogenesis

BONE, 41(6), 1036–1044.

By: D. Yan *, A. Gurumurth*, M. Wright n, T. Pfeiler n, E. Loboa n & E. Everett *

author keywords: F; osteoclastogenesis; hematopoietic colony-forming cell (CFC) assays; bone resorption markers; inbred mouse strains
MeSH headings : Alkaline Phosphatase / metabolism; Animals; Biomarkers / blood; Bone Density; Bone and Bones / cytology; Bone and Bones / metabolism; Colony-Forming Units Assay; Female; Fluorides / metabolism; Fluorides / pharmacology; Mice; Osteoclasts / drug effects; Osteoclasts / metabolism; Osteogenesis / drug effects; Osteogenesis / genetics; Stress, Mechanical
TL;DR: In conclusion, short term F treatment at physiological levels has strain-specific effects in mice and novel actions hallmarked by enhanced osteoclastogenesis shifts in hematopoietic cell differentiation in the C3H strain are demonstrated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Human adipose-derived adult stem cells upregulate palladin during osteogenesis and in response to cyclic tensile strain

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 293(5), C1532–C1538.

By: M. Wall n, A. Rachlin, C. Otey* & E. Loboa*

author keywords: adipose-derived adult stem cells; mechanobiology; Eps8; actin cytoskeleton; mesenchymal stem cells
MeSH headings : Actins / metabolism; Adaptor Proteins, Signal Transducing; Adipose Tissue / cytology; Adipose Tissue / metabolism; Adult; Adult Stem Cells / metabolism; Cell Lineage / genetics; Cell Shape; Cells, Cultured; Cytoskeletal Proteins / genetics; Cytoskeletal Proteins / metabolism; Female; Humans; Intracellular Signaling Peptides and Proteins / metabolism; Mechanotransduction, Cellular / genetics; Middle Aged; Osteogenesis / genetics; Phosphoproteins / genetics; Phosphoproteins / metabolism; Protein Isoforms / metabolism; RNA Interference; RNA, Messenger / metabolism; RNA, Small Interfering / metabolism; Stress Fibers / metabolism; Stress, Mechanical; Up-Regulation
TL;DR: It is shown that palladin was expressed by hADAS cells and was modulated during osteogenic differentiation and in response to cyclic tensile strain, and that knocking down the palladin gene resulted in decreased actin stress fibers and decreased protein levels of Eps8. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Semiautomated finite element mesh generation methods for a long bone

COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE, 85(3), 196–202.

By: T. Pfeiler n, D. Lalush n & E. Loboa n

author keywords: canine radius; CT to FEA; four-point bending; strain gage
MeSH headings : Animals; Bone and Bones / diagnostic imaging; Cadaver; Computer Simulation; Dogs; Finite Element Analysis; North Carolina; Tomography, X-Ray Computed
TL;DR: A semi-automated finite element mesh generation method using computed tomography image data of a canine radius is developed and test to minimize user interaction and eliminate the need for mesh smoothing to produce a model suitable for finite element analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
5. Gender Equality (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2006 article

Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: Effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression

Sumanasinghe, R. D., Bernacki, S. H., & Loboa, E. G. (2006, December). TISSUE ENGINEERING, Vol. 12, pp. 3459–3465.

By: R. Sumanasinghe n, S. Bernacki n & E. Loboa n

MeSH headings : Adult; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins / biosynthesis; Bone Morphogenetic Proteins / genetics; Bone and Bones / cytology; Bone and Bones / metabolism; Cell Differentiation / physiology; Cell Survival / physiology; Cells, Cultured; Collagen Type I; Gene Expression Regulation, Developmental / physiology; Humans; Male; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / metabolism; RNA, Messenger / biosynthesis; Tensile Strength; Transforming Growth Factor beta / biosynthesis; Transforming Growth Factor beta / genetics
TL;DR: This is the first report of the culture of bone marrow-derived hMSCs in 3D collagen matrices under cyclic strain, and the first demonstration that strain alone can induce osteogenic differentiation without the addition of osteogenic supplements. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Mechanobiology of mandibular distraction osteogenesis: finite element analyses with a rat model

JOURNAL OF ORTHOPAEDIC RESEARCH, 23(3), 663–670.

By: E. Loboa*, T. Fang *, D. Parker*, S. Warren*, K. Fong*, M. Longaker *, D. Carter*

author keywords: mechanobiology; mandibular distraction osteogenesis; finite element analysis; computed tomography; mesenchymal tissue differentiation
MeSH headings : Animals; Finite Element Analysis; Hydrostatic Pressure; Male; Mandible / physiology; Mandible / surgery; Osteogenesis, Distraction; Rats; Rats, Sprague-Dawley; Tensile Strength
TL;DR: Finite element analyses performed at different time points during distraction were instrumental in determining the changes in hydrostatic stress and tensile strain fields throughout distraction, providing a mechanical environment rationale for the different levels of bone formation in end latency, and distraction day 2, 5, and 8 specimens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Mechanobiology of mandibular distraction osteogenesis: experimental analyses with a rat model

BONE, 34(2), 336–343.

By: E. Loboa*, T. Fang *, S. Warren*, D. Lindsey *, K. Fong*, M. Longaker *, D. Carter*

author keywords: mandibular distraction osteogenesis; mechanobiology; rat model; mechanical testing; mesenchymal tissue
MeSH headings : Animals; Biomechanical Phenomena; Male; Mandible / pathology; Mandible / surgery; Osteogenesis / physiology; Osteogenesis, Distraction / methods; Rats; Rats, Sprague-Dawley; Stress, Mechanical; Tensile Strength
TL;DR: It is concluded that strains within this range provide an excellent environment for de novo bone apposition and daily distractions cause daily tissue damage which triggers new mesenchymal tissue formation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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