Michael Scott Price
Works (4)
Updated: July 5th, 2023 15:58
2007 journal article
Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus
BMC MICROBIOLOGY, 7.
MeSH headings : Antifungal Agents / pharmacology; Aspergillus flavus / genetics; Blotting, Southern; Drug Resistance, Fungal / genetics; Genes, Fungal; Genetic Complementation Test; Mutagenesis; Mutagenesis, Insertional; Orotic Acid / analogs & derivatives; Orotic Acid / metabolism; Phleomycins / pharmacology; Polymerase Chain Reaction / methods; Protoplasts; Spores, Fungal / genetics; Transformation, Genetic; Uracil / biosynthesis
TL;DR:
The phleomycin resistance as a new positive selectable marker for genetic transformation of A. flavus allows strains to be transformed without having to generate an auxotrophic mutation, which is time consuming and may result in undesirable mutations.
(via Semantic Scholar)
open access via bmcmicrobiol.biomedcentral.com (publisher PDF)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
The aflatoxin pathway regulator AfIR induces gene transcription inside and outside of the aflatoxin biosynthetic cluster
FEMS MICROBIOLOGY LETTERS, 255(2), 275–279.
author keywords: transcription profiling; microarray analysis; aflatoxin; secondary metabolism; Aspergillus flavus; Aspergillus parasiticus
MeSH headings : Aflatoxins / biosynthesis; Aspergillus / genetics; Aspergillus / metabolism; Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Regulator; Multigene Family; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction / methods; Transcription, Genetic
TL;DR:
This report is the first to demonstrate genes outside the biosynthetic cluster as being associated with aflR expression in Aspergillus parasiticus strains grown under conditions conducive for aflatoxin production.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
Aflatoxin conducive and non-conducive growth conditions reveal new gene associations with aflatoxin production
FUNGAL GENETICS AND BIOLOGY, 42(6), 506–518.
author keywords: aflatoxin; gene expression; regulation; Aspergillus flavus; Aspergillus parasiticus
MeSH headings : Aflatoxins / biosynthesis; Aspergillus / genetics; Aspergillus / metabolism; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Fungal; Genes, Fungal; Hydrogen-Ion Concentration; Nitrogen / metabolism; Oligonucleotide Array Sequence Analysis; RNA, Fungal / analysis; RNA, Messenger / analysis; Regulon; Temperature
TL;DR:
To understand the effect of nitrogen source, carbon source, temperature, and pH on the regulation of AF biosynthesis, a targeted cDNA microarray consisting of genes associated with AF production over time was employed.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2004 journal article
A chitinase from Tex6 maize kernels inhibits growth of Aspergillus flavus
PHYTOPATHOLOGY, 94(1), 82–87.
TL;DR:
The Tex6 chitinase purified in this study is capable of inhibiting the growth of A. flavus by 50% at a concentration of 20 mug/ml and makes a major contribution to the antifungal activity in this maize genotype.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
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