@article{he_price_obrian_georgianna_payne_2007, title={Improved protocols for functional analysis in the pathogenic fungus Aspergillus flavus}, volume={7}, ISSN={["1471-2180"]}, DOI={10.1186/1471-2180-7-104}, abstractNote={Abstract}, journal={BMC MICROBIOLOGY}, author={He, Zhu-Mei and Price, Michael S. and OBrian, Gregory R. and Georgianna, D. Ryan and Payne, Gary A.}, year={2007}, month={Nov} }
 @article{price_yu_nierman_kim_pritchard_jacobus_bhatnagar_cleveland_payne_2006, title={The aflatoxin pathway regulator AfIR induces gene transcription inside and outside of the aflatoxin biosynthetic cluster}, volume={255}, ISSN={["1574-6968"]}, DOI={10.1111/j.1574-6968.2005.00084.x}, abstractNote={Aflatoxin contamination of food and feed is a major concern due to the carcinogenic properties of this mycotoxin. Previous studies using classical approaches have identified a cluster of genes responsible for aflatoxin production under the control of the pathway-specific transcriptional regulator aflR, but it is unknown whether aflR controls expression of other genes within the genome. Transcription profiling comparing wild type and DeltaaflR strains of Aspergillus parasiticus grown under conditions conducive for aflatoxin production identified only 23 upregulated genes in the wild type. These included 20 genes in the aflatoxin biosynthetic cluster, and three additional genes outside the aflatoxin biosynthetic cluster (nadA, hlyC, and niiA), all with AflR binding sites. This report is the first to demonstrate genes outside the biosynthetic cluster as being associated with aflR expression.}, number={2}, journal={FEMS MICROBIOLOGY LETTERS}, author={Price, MS and Yu, JJ and Nierman, WC and Kim, HS and Pritchard, B and Jacobus, CA and Bhatnagar, D and Cleveland, TE and Payne, GA}, year={2006}, month={Feb}, pages={275–279} }
 @article{price_shannon_sabrina_robert_payne_2005, title={Aflatoxin conducive and non-conducive growth conditions reveal new gene associations with aflatoxin production}, volume={42}, ISSN={["1096-0937"]}, DOI={10.1016/j.fgb.2005.03.009}, abstractNote={Research on aflatoxin (AF) production has traditionally focused on defining the AF biosynthetic pathway with the goal of identifying potential targets for intervention. To understand the effect of nitrogen source, carbon source, temperature, and pH on the regulation of AF biosynthesis, a targeted cDNA microarray consisting of genes associated with AF production over time was employed. Expression profiles for genes involved in AF biosynthesis grouped into five clades. A putative regulon was identified consisting of 20 genes that were induced in the conducive nitrogen and pH treatments and the non-conducive carbon and temperature treatments, as well as four other putative regulons corresponding to each of the four variables studied. Seventeen genes exhibited consistent induction/repression profiles across all the experiments. One of these genes was consistently downregulated with AF production. Overexpression of this gene resulted in repression of AF biosynthesis. The cellular function of this gene is currently unresolved.}, number={6}, journal={FUNGAL GENETICS AND BIOLOGY}, author={Price, MS and Shannon, BCB and Sabrina, TB and Robert, AKB and Payne, GA}, year={2005}, month={Jun}, pages={506–518} }
 @article{moore_price_boston_weissinger_payne_2004, title={A chitinase from Tex6 maize kernels inhibits growth of Aspergillus flavus}, volume={94}, ISSN={["1943-7684"]}, DOI={10.1094/PHYTO.2004.94.1.82}, abstractNote={ The maize inbred Tex6 has resistance to colonization and aflatoxin accumulation by Aspergillus flavus. A protein inhibitory to growth of A. flavus has been identified from aqueous extracts of mature Tex6 seeds. This study reports the purification of a chitinase associated with this inhibitory activity to electrophoretic homogeneity and the further characterization of its properties. The inhibitory protein, which has an Mr of 29,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, is an endochitinase that is also capable of exochitinase activity. The enzyme has an optimal pH of 5.5 and a temperature optimum of 45°C. Chitinase activity in maize kernels peaked approximately 36 days after pollination. The Tex6 chitinase purified in this study is capable of inhibiting the growth of A. flavus by 50% at a concentration of 20 μg/ml. Our data indicate that chitinase activity in Tex6 kernels makes a major contribution to the antifungal activity in this maize genotype. Partial peptide sequence of the chitinase showed it to differ from previously reported chitinases. }, number={1}, journal={PHYTOPATHOLOGY}, author={Moore, KG and Price, MS and Boston, RS and Weissinger, AK and Payne, GA}, year={2004}, month={Jan}, pages={82–87} }