Christopher P. Wilcox

Works (5)

Updated: July 5th, 2023 16:04

2002 journal article

Immobilization and utilization of the recombinant fusion proteins trypsin-streptavidin and streptavidin-transglutaminase for modification of whey protein isolate functionality

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50(13), 3723–3730.

By: C. Wilcox n, D. Clare n, V. Valentine n & H. Swaisgood n

author keywords: immobilization; trypsin; transglutaminase; whey protein; functionality
MeSH headings : Adsorption; Biotinylation; Chemical Phenomena; Chemistry, Physical; Cross-Linking Reagents; DNA, Recombinant; Dithiothreitol / pharmacology; Enzymes, Immobilized; Escherichia coli / genetics; Gels; Glass; Milk Proteins / chemistry; Milk Proteins / metabolism; Recombinant Fusion Proteins; Streptavidin / genetics; Temperature; Transglutaminases / genetics; Transglutaminases / metabolism; Trypsin / genetics; Trypsin / metabolism; Viscosity; Whey Proteins
TL;DR: By combining limited proteolysis with controlled cross-linking, it was possible to create derivatives of whey proteins with enhanced functional properties that allowed for recycling of the enzyme, eliminated the requirement for a downstream inactivation step, and permitted control over the extent of modification. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2002 journal article

Isolation and partial characterization of CD36 from skim milk

JOURNAL OF DAIRY SCIENCE, 85(8), 1903–1908.

By: C. Wilcox n, V. Janolino n & H. Swaisgood n

author keywords: CD36; milk fat globule membrane; skim milk; sulfhydryl oxidase
MeSH headings : Amino Acid Sequence; Animals; Blotting, Western; CD36 Antigens; Cattle; Centrifugation; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Glycolipids / analysis; Glycoproteins / analysis; Glycosylation; Lipid Droplets; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / isolation & purification; Milk / chemistry; Molecular Sequence Data; Molecular Weight; Organic Anion Transporters / chemistry; Organic Anion Transporters / isolation & purification; Ultrafiltration
TL;DR: CD36, a common milk fat globule membrane glycoprotein, was isolated from skim milk by methods similar to those previously utilized for the isolation of sulfhydryl oxidase, giving similar purity as observed by electrophoresis and suggested tissue-specific glycosylation. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2002 journal article

Modification of the rheological properties of whey protein isolate through the use of an immobilized microbial transglutaminase

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50(20), 5546–5551.

By: C. Wilcox n & H. Swaisgood n

author keywords: immobilization; transglutaminase; viscosity; whey protein isolate; gelation; cross linking
MeSH headings : Adsorption; Avidin / chemistry; Bacteria / enzymology; Biotinylation; Cross-Linking Reagents; Enzymes, Immobilized; Glass; Microspheres; Milk Proteins / chemistry; Rheology; Solutions; Transglutaminases / metabolism; Viscosity; Whey Proteins
TL;DR: The functional properties of several batches of WPI were modified using <10 mg of the same enzyme, illustrating the capacity of immobilized enzymes to be used more frequently in applications of this nature. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1997 journal article

Production and purification of an active bovine lysozyme in tobacco (Nicotiana tabacum): Utilization of value-added crop plants traditionally grown under intensive agriculture

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 45(7), 2793–2797.

By: C. Wilcox n, A. Weissinger n, R. Long n, L. Fitzmaurice n, T. Mirkov n & H. Swaisgood n

author keywords: transgenic; lysozyme; recombinant protein; antimicrobial; Nicotiana tabacum
TL;DR: It was concluded that tobacco was an excellent choice for expression of the recombinant protein and that the purification process developed in this study demonstrates methodology for isolation of high-value enzymes from tobacco and other crop plants. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

1993 conference paper

Application of genetic markers to tree breeding

Proceedings of the 22nd Southern Forest Tree Improvement Conference, 452–463.

By: D. Grattapaglia, J. Chaparro, P. Wilcox, S. McCord, B. Crane, H. Amerson, D. Werner, B. Liu ...

Source: NC State University Libraries
Added: August 6, 2018

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