@article{tremblay_vaewhongs_turner_sit_lommel_2005, title={Cell wall localization of Red clover necrotic mosaic virus movement protein is required for cell-to-cell movement}, volume={333}, ISSN={["0042-6822"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-13644249879&partnerID=MN8TOARS}, DOI={10.1016/j.virol.2004.12.019}, abstractNote={The Red clover necrotic mosaic virus movement protein (MP) is essential for cell-to-cell movement. Eight previously characterized alanine-scanning mutants of the MP were fused to the green fluorescent protein (GFP) and expressed from viral infectious transcripts. Inoculated plants were assayed for movement and intracellular accumulation of MP by confocal laser-scanning microscopy. A strict correlation was observed between the targeting to the cell wall (presumably the plasmodesmata) and cell-to-cell movement. Complementation of dysfunctional MP mutants with either wild-type MP or other null mutants in some cases rescued intracellular targeting and movement. The data suggest the presence of distinct domains in the MP for virus movement (near residues 27–31), complementarity (near residues 122 and 128), and intracellular localization (near residue 161). These data support a model of MP interacting cooperatively with itself to bind viral RNA, localize to and modify plasmodesmata and effect virus movement.}, number={1}, journal={VIROLOGY}, publisher={Elsevier BV}, author={Tremblay, D and Vaewhongs, AA and Turner, KA and Sit, TL and Lommel, SA}, year={2005}, month={Mar}, pages={10–21} }
 @article{vaewhongs_lommel_1995, title={VIRION FORMATION IS REQUIRED FOR THE LONG-DISTANCE MOVEMENT OF RED-CLOVER NECROTIC MOSAIC-VIRUS IN MOVEMENT PROTEIN TRANSGENIC PLANTS}, volume={212}, ISSN={["0042-6822"]}, DOI={10.1006/viro.1995.1518}, abstractNote={The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species. The polycistronic RNA-1 encodes the viral RNA polymerase and capsid protein (CP) and the monocistronic RNA-2 encodes the 35 kDa cell-to-cell movement protein (MP). Nicotiana benthamiana plants transformed with the RCNMV MP gene were generated. When inoculated onto the MP transgenic plants, cell-to-cell movement of RNA-1 occurred at a rate similar to wild-type virus. However, long-distance (leaf-to-leaf) movement of RNA-1 was not observed. Neither CP nor virions were detected in the inoculated leaves of the MP transgenic plants. When RNA-1 was coinoculated with RNA-2 mutants, which do not express a functional MP, onto MP transgenic plants, CP and virions were readily detected and a systemic infection resulted. These results demonstrate that both RNA-1 and RNA-2 are necessary for the accumulation of both CP and virions. Furthermore, CP accumulation was found to be required for long-distance movement of RCNMV. Therefore, these data provide evidence that CP, in the form of virions, is necessary for the long-distance movement of RCNMV.}, number={2}, journal={VIROLOGY}, author={VAEWHONGS, AA and LOMMEL, SA}, year={1995}, month={Oct}, pages={607–613} }