@article{xi_patel_dong_que_qu_2018, title={Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus}, volume={18}, ISSN={["1472-6750"]}, DOI={10.1186/s12896-018-0459-5}, abstractNote={Large T-DNA fragment transfer has long been a problem for Agrobacterium-mediated transformation. Although vector systems, such as the BIBAC series, were successfully developed for the purpose, low transformation efficiencies were consistently observed. To gain insights of this problem in monocot transformation, we investigated the T-strand accumulation of various size of T-DNA in two kinds of binary vectors (one copy vs. multi-copy) upon acetosyringone (AS) induction and explored ways to improve the efficiency of the large T-DNA fragment transfer in Agrobacterium-mediated rice transformation. By performing immuno-precipitation of VirD2-T-strands and quantitative real-time PCR assays, we monitored the accumulation of the T-strands in Agrobacterium tumeficiens after AS induction. We further demonstrated that extension of AS induction time highly significantly improved large-size T-DNA transfer to rice cells. Our data provide valuable information of the T-strand dynamics and its impact on large T-DNA transfer in monocots, and likely dicots as well.}, journal={BMC BIOTECHNOLOGY}, author={Xi, Jing and Patel, Minesh and Dong, Shujie and Que, Qiudeng and Qu, Rongda}, year={2018}, month={Aug} }
 @article{dong_shew_tredway_lu_sivamani_miller_qu_2008, title={Expression of the bacteriophage T4 lysozyme gene in tall fescue confers resistance to gray leaf spot and brown patch diseases}, volume={17}, ISSN={["1573-9368"]}, DOI={10.1007/s11248-007-9073-3}, abstractNote={Tall fescue (Festuca arundinacea Schreb.) is an important turf and forage grass species worldwide. Fungal diseases present a major limitation in the maintenance of tall fescue lawns, landscapes, and forage fields. Two severe fungal diseases of tall fescue are brown patch, caused by Rhizoctonia solani, and gray leaf spot, caused by Magnaporthe grisea. These diseases are often major problems of other turfgrass species as well. In efforts to obtain tall fescue plants resistant to these diseases, we introduced the bacteriophage T4 lysozyme gene into tall fescue through Agrobacterium-mediated genetic transformation. In replicated experiments under controlled environments conducive to disease development, 6 of 13 transgenic events showed high resistance to inoculation of a mixture of two M. grisea isolates from tall fescue. Three of these six resistant plants also displayed significant resistance to an R. solani isolate from tall fescue. Thus, we have demonstrated that the bacteriophage T4 lysozyme gene confers resistance to both gray leaf spot and brown patch diseases in transgenic tall fescue plants. The gene may have wide applications in engineered fungal disease resistance in various crops.}, number={1}, journal={TRANSGENIC RESEARCH}, author={Dong, Shujie and Shew, H. David and Tredway, Lane P. and Lu, Jianli and Sivamani, Elumalai and Miller, Eric S. and Qu, Rongda}, year={2008}, month={Feb}, pages={47–57} }
 @article{dong_tredway_shew_wang_sivamani_qu_2007, title={Resistance of transgenic tall fescue to two major fungal diseases}, volume={173}, ISSN={["0168-9452"]}, DOI={10.1016/j.plantsci.2007.08.002}, abstractNote={Tall fescue (Festuca arundinacea Schreb.) is an open-pollinated, perennial, cool-season turf and forage grass species of great economic importance. The main problems of maintenance of tall fescue, and many other turfgrasses, are two severe fungal diseases: gray leaf spot caused by Magnaporthe grisea, and brown patch caused by Rhizoctonia solani. Three genes from various sources have been introduced into two elite cultivars, ‘Coronado’ and ‘Matador’, of tall fescue through Agrobacterium-mediated transformation, and conferred resistance to the diseases. Two genes, the alfalfa β-1,3-glucanase AGLU1 gene and a truncated frog dermaseptin SI gene, conferred resistance to both diseases. The rice Pi9 gene was specific against gray leaf spot. Of 15 T0 transgenic plants examined, 6 showed high levels of resistance to M. grisea, and 3 had enhanced resistance to R. solani. The resistance in most of these cases was highly significant.}, number={5}, journal={PLANT SCIENCE}, author={Dong, Shujie and Tredway, Lane P. and Shew, H. David and Wang, Guo-Liang and Sivamani, Elumalai and Qu, Rongda}, year={2007}, month={Nov}, pages={501–509} }
 @article{dong_qu_2005, title={High efficiency transformation of tall fescue with Agrobacterium tumefaciens}, volume={168}, ISSN={["0168-9452"]}, DOI={10.1016/j.plantsci.2005.01.008}, abstractNote={An efficient genetic transformation system for tall fescue (Festuca arundinacea Schreb.), using Agrobacterium tumefaciens-mediated T-DNA delivery, is described. Seed-derived embryogenic calli were infected with Agrobacterium tumefaciens strain EHA105 harboring plasmids pTOK47 and pCAMBIA1301. Infected calli were selected at 250 mg L−1 hyg B and the regenerated plantlets at 50 mg L−1. Using the protocol developed, 34% of the calli infected were hyg B resistant, and the overall plant transformation frequency (number of independently transformed plants over number of calli infected) was 8%. Stable integration of transgene into plant genome and GUS reporter gene expression were confirmed. Low transgene copies (1 or 2 as estimated) were observed in all the transgenic plants analyzed, and the transgene was transmitted to the progenies. Further experiments suggest an elevated 2,4-D concentration (5 mg L−1) used during callus culture and co-cultivation was partially attributable to the observed high transformation efficiency, while the inclusion of plasmid pTOK47 in Agrobacterium was not. The whole process from callus induction to transplanting green plantlets to the soil takes about six months, significantly shorter than the suspension culture approach often used in tall fescue transformation.}, number={6}, journal={PLANT SCIENCE}, author={Dong, SJ and Qu, RD}, year={2005}, month={Jun}, pages={1453–1458} }