Donald Andrew Comfort

Works (7)

Updated: July 5th, 2023 15:56

2010 journal article

A novel alpha-d-galactosynthase from Thermotoga maritima converts beta-d-galactopyranosyl azide to alpha-galacto-oligosaccharides

GLYCOBIOLOGY, 21(4), 448–456.

By: B. Cobucci-Ponzano *, C. Zorzetti*, A. Strazzulli *, S. Carillo *, E. Bedini *, M. Corsaro *, D. Comfort n, R. Kelly n , M. Rossi*, M. Moracci *

author keywords: alpha-galacto-oligosaccharides; glycosynthase; alpha-gal epitope; carbohydrate synthesis; protein engineering
MeSH headings : Aspartic Acid / chemistry; Azides / chemistry; Chromatography, Thin Layer; Disaccharides / biosynthesis; Galactose / metabolism; Glycine / chemistry; Kinetics; Mutagenesis, Site-Directed; Mutant Proteins / chemistry; Mutant Proteins / genetics; Mutant Proteins / metabolism; Protein Engineering; Thermotoga maritima / enzymology; alpha-Galactosidase / chemistry; alpha-Galactosidase / genetics; alpha-Galactosidase / metabolism
TL;DR: Results open a new avenue to the practical synthesis of biologically interesting α-galacto-oligosaccharides and demonstrate more widespread use of β-glycosyl-azide as donors, confirming their utility to expand the repertoire of glycosynthases. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Biochemical analysis of Thermotoga maritima GH36 alpha-galactosidase (TmGalA) confirms the mechanistic commonality of clan GH-D glycoside hydrolases

BIOCHEMISTRY, 46(11), 3319–3330.

By: D. Comfort n, K. Bobrov  n, D. Ivanen n, K. Shabalin  n, J. Harris n, A. Kulminskaya  n, H. Brumer  n, R. Kelly n 

MeSH headings : Amino Acid Sequence; Azides / pharmacology; Catalysis; Cloning, Molecular; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Denaturation; Sequence Alignment; Substrate Specificity; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; alpha-Galactosidase / drug effects; alpha-Galactosidase / genetics; alpha-Galactosidase / metabolism
TL;DR: Results show that the biochemical characteristics of GH36 TmGalA are closely related to GH27 enzymes, confirming the mechanistic commonality of clan GH-D members, and confirm that Asp387 is the acid/base residue of Tm GalA. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Functional-genomics-based identification and characterization of open reading frames encoding alpha-glucoside-processing enzymes in the hyperthermophilic archaeon Pyrococcus furiosus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(4), 1281–1283.

By: D. Comfort n, C. Chou n, S. Conners n, A. VanFossen n & R. Kelly n 

MeSH headings : Computational Biology; Gene Expression Regulation, Bacterial / genetics; Genomics / methods; Open Reading Frames / genetics; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; alpha-Glucosidases / genetics; beta-Amylase / genetics
TL;DR: Bioinformatics analysis and transcriptional response information for Pyrococcus furiosus led to the identification of a novel isomaltase representing a new glycoside hydrolase family, a novel GH57 β-amylase, and an extracellular starch-binding protein. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Impact of substrate glycoside linkage. and elemental sulfur on bioenergetics, of and hydrogen production by the hyperthermophilic Archaeon Pyrococcus furiosus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(21), 6842–6853.

By: C. Chou n, K. Shockley n, S. Conners n, D. Lewis n, D. Comfort n, M. Adams  n, R. Kelly n 

MeSH headings : Archaea; Chemical Phenomena; Chemistry; Gene Expression Regulation, Archaeal; Genome, Archaeal; Glycosides / metabolism; Hot Temperature; Hydrogen / metabolism; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / physiology; Sulfur / metabolism
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Rheological properties of guar galactomannan solutions during hydrolysis with galactomannanase and alpha-galactosidase enzyme mixtures

BIOMACROMOLECULES, 8(3), 949–956.

By: S. Mahammad n, D. Comfort n, R. Kelly n  & S. Khan n 

MeSH headings : Carbohydrate Conformation; Galactans / metabolism; Galactose / analogs & derivatives; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Mannans / chemistry; Mannans / metabolism; Mannose / chemistry; Mannosidases / chemistry; Models, Chemical; Models, Statistical; Plant Gums / metabolism; Polysaccharides / chemistry; Rheology / methods; Time Factors; alpha-Galactosidase / chemistry
TL;DR: A rheology-based kinetic model is developed to estimate the reaction rate constants and interpret synergistic effects of multiple enzyme contributions and reveals that both the native and the debranched guar have the same activation energy for beta-mannanase action, although debranching considerably increases the frequency of enzyme-guar interactions. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus

JOURNAL OF BACTERIOLOGY, 188(6), 2115–2125.

By: H. Lee *, K. Shockley n, G. Schut *, S. Conners n, C. Montero n, M. Johnson n, C. Chou n, S. Bridger n ...

MeSH headings : Archaeal Proteins / genetics; Bacterial Proteins / genetics; DNA, Archaeal / chemistry; DNA, Archaeal / genetics; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Glucose / metabolism; Glucosephosphates / metabolism; Glucosyltransferases / genetics; Glycogen Debranching Enzyme System / genetics; Glycoside Hydrolases / genetics; Monosaccharide Transport Proteins / genetics; Oligonucleotide Array Sequence Analysis; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Sequence Analysis, DNA; Starch / metabolism; Substrate Specificity
TL;DR: Starch metabolism in P. furiosus was examined by biochemical analyses in conjunction with global transcriptional response data for cells grown on a variety of glucans, and several hypothetical proteins encoded by three gene clusters are also involved in the transport and processing of alpha-glucan substrates by this organism. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima

JOURNAL OF BACTERIOLOGY, 187(21), 7267–7282.

By: S. Conners n, C. Montero n, D. Comfort n, K. Shockley n, M. Johnson n, . Chhabra n, R. Kelly n 

MeSH headings : 5' Flanking Region / genetics; ATP-Binding Cassette Transporters / chemistry; ATP-Binding Cassette Transporters / genetics; Biological Evolution; Biological Transport / genetics; Carbohydrate Metabolism; Computational Biology; Gene Duplication; Gene Expression Profiling; Genes, Bacterial; Genes, Regulator; Genomics; Monosaccharide Transport Proteins / genetics; Nucleic Acid Conformation; Oligosaccharides / metabolism; Pentose Phosphate Pathway / genetics; Regulon / physiology; Rhamnose / metabolism; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Xylose / metabolism
TL;DR: The results indicate that T. maritima relies extensively on ABC transporters for carbohydrate uptake, many of which are likely controlled by local regulators responsive to either the transport substrate or a key metabolic degradation product. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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