@misc{onstott_mcgown_bakermans_ruskeeniemi_ahonen_telling_soffientino_pfiffner_sherwood-lollar_frape_et al._2009, title={Microbial communities in subpermafrost saline fracture water at the Lupin Au Mine, Nunavut, Canada}, volume={58}, number={4}, journal={Microbial Ecology}, author={Onstott, T. C. and McGown, D. J. and Bakermans, C. and Ruskeeniemi, T. and Ahonen, L. and Telling, J. and Soffientino, B. and Pfiffner, S. M. and Sherwood-Lollar, B. and Frape, S. and et al.}, year={2009}, pages={786–807} } @article{vishnivetskaya_kathariou_tiedje_2009, title={The Exiguobacterium genus: biodiversity and biogeography}, volume={13}, ISSN={["1433-4909"]}, DOI={10.1007/s00792-009-0243-5}, abstractNote={Bacteria of the genus Exiguobacterium are low G + C, Gram-positive facultative anaerobes that have been repeatedly isolated from ancient Siberian permafrost. In addition, Exiguobacterium spp. have been isolated from markedly diverse sources, including Greenland glacial ice, hot springs at Yellowstone National Park, the rhizosphere of plants, and the environment of food processing plants. Strains of this hereto little known bacterium that have been retrieved from such different (and often extreme) environments are worthy of attention as they are likely to be specifically adapted to such environments and to carry variations in the genome which may correspond to psychrophilic and thermophilic adaptations. However, comparative genomic investigations of Exiguobacterium spp. from different sources have been limited. In this study, we employed different molecular approaches for the comparative analysis of 24 isolates from markedly diverse environments including ancient Siberian permafrost and hot springs at Yellowstone National Park. Pulsed-field gel electrophoresis (PFGE) with I-CeuI (an intron-encoded endonuclease), AscI and NotI were optimized for the determination of genomic fingerprints of nuclease-producing isolates. The application of a DNA macroarray for 82 putative stress-response genes yielded strain-specific hybridization profiles. Cluster analyses of 16S rRNA gene sequence data, PFGE I-CeuI restriction patterns and hybridization profiles suggested that Exiguobacterium strains formed two distinct divisions that generally agreed with temperature ranges for growth. With few exceptions (e.g., Greenland ice isolate GIC31), psychrotrophic and thermophilic isolates belonged to different divisions.}, number={3}, journal={EXTREMOPHILES}, author={Vishnivetskaya, Tatiana A. and Kathariou, Sophia and Tiedje, James M.}, year={2009}, month={May}, pages={541–555} } @article{vishnivetskaya_siletzky_jefferies_tiedje_kathariou_2007, title={Effect of low temperature and culture media on the growth and freeze-thawing tolerance of Exiguobacterium strains}, volume={54}, ISSN={["0011-2240"]}, DOI={10.1016/j.cryobiol.2007.01.008}, abstractNote={Bacteria of the genus Exiguobacterium have been repeatedly isolated from ancient permafrost sediments of the Kolyma lowland of Northeast Eurasia. Here we report that the Siberian permafrost isolates Exiguobacterium sibiricum 255-15, E. sibiricum 7-3, Exiguobacterium undae 190-11 and E. sp. 5138, as well as Exiguobacterium antarcticum DSM 14480, isolated from a microbial mat sample of Lake Fryxell (McMurdo Dry Valleys, Antarctica), were able to grow at temperatures ranging from −6 to 40 °C. In comparison to cells grown at 24 °C, the cold-grown cells of these strains tended to be longer and wider. We also investigated the effect of growth conditions (broth or surface growth, and temperature) on cryotolerance of the Exiguobacterium strains. Bacteria grown in broth at 4 °C showed markedly greater survival following freeze-thawing treatments (20 repeated cycles) than bacteria grown in broth at 24 °C. Surprisingly, significant protection to repeated freeze-thawing was also observed when bacteria were grown on agar at either 4 or 24 °C.}, number={2}, journal={CRYOBIOLOGY}, author={Vishnivetskaya, Tatiana A. and Siletzky, Robin and Jefferies, Natalie and Tiedje, James M. and Kathariou, Sophia}, year={2007}, month={Apr}, pages={234–240} } @article{bakermans_ayala-del-rio_ponder_vishnivetskaya_gilichinsky_thomashow_tiedje_2006, title={Psychrobacter cryohalolentis sp nov and Psychrobacter arcticus sp nov., isolated from Siberian permafrost}, volume={56}, ISSN={["1466-5026"]}, DOI={10.1099/ijs.0.64043-0}, abstractNote={Three Gram-negative, non-motile, non-pigmented, oxidase-positive coccobacilli capable of growth at temperatures from −10 to 30 °C and salinities of 0 to 1.7 M NaCl were isolated from Siberian permafrost and characterized. Both 16S rRNA and gyrB gene sequencing studies placed the isolates in the Gammaproteobacteria within the genus Psychrobacter. However, with higher bootstrap values and reproducible tree topologies, gyrB represented a more reliable phylogenetic marker for the taxonomy of Psychrobacter species. DNA–DNA hybridization data supported gyrB tree topologies and established two relatedness groups within the three isolates; neither of these groups was related at the species level to any previously described Psychrobacter species. The two groups of isolates could be differentiated phenotypically from 13 previously described Psychrobacter species using API strips. These results support the existence of two novel species of Psychrobacter, for which we propose the names Psychrobacter cryohalolentis sp. nov. (type strain K5T=DSM 17306T=VKM B-2378T) and Psychrobacter arcticus sp. nov. (type strain 273-4T=DSM 17307T=VKM B-2377T).}, journal={INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}, author={Bakermans, C and Ayala-del-rio, HL and Ponder, MA and Vishnivetskaya, T and Gilichinsky, D and Thomashow, MF and Tiedje, JM}, year={2006}, month={Jun}, pages={1285–1291} } @article{vishnivetskaya_kathariou_2005, title={Putative transposases conserved in Exiguobacterium isolates from ancient Siberian permafrost and from contemporary surface habitats}, volume={71}, ISSN={["1098-5336"]}, DOI={10.1128/AEM.71.11.6954-6962.2005}, abstractNote={ABSTRACT}, number={11}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Vishnivetskaya, TA and Kathariou, S}, year={2005}, month={Nov}, pages={6954–6962} }