Works (17)
Updated: July 5th, 2023 15:56
2017 journal article
Impact of growth mode, phase, and rate on the metabolic state of the extremely thermophilic archaeon Pyrococcus furiosus
BIOTECHNOLOGY AND BIOENGINEERING, 114(12), 2947–2954.
author keywords: continuous culture; growth phase; growth rate; hyperthermophiles; Pyrococcus furiosus; transcriptome
MeSH headings : Archaeal Proteins / metabolism; Batch Cell Culture Techniques / methods; Cell Proliferation / physiology; Energy Metabolism / physiology; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Transcriptome / physiology
TL;DR:
The results here suggest that cellular processes involved in carbon and electron flux in P. furiosus were significantly impacted by growth mode, phase and rate, factors that need to be taken into account when developing successful metabolic engineering strategies.
(via Semantic Scholar)
open access via europepmc.org (repository)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2014 journal article
Control of Protein Function through Optochemical Translocation
ACS SYNTHETIC BIOLOGY, 3(10), 731–736.
author keywords: protein control; optogenetics; nuclear import; photocontrolled transcription; photocontrolled TEV-cleavage
MeSH headings : Active Transport, Cell Nucleus / radiation effects; Amino Acid Sequence; Endopeptidases / genetics; Endopeptidases / metabolism; Endopeptidases / radiation effects; Forkhead Box Protein O3; Forkhead Transcription Factors / genetics; Forkhead Transcription Factors / metabolism; Forkhead Transcription Factors / radiation effects; HEK293 Cells; Humans; Light; Matrix Attachment Region Binding Proteins / genetics; Matrix Attachment Region Binding Proteins / metabolism; Matrix Attachment Region Binding Proteins / radiation effects; Molecular Sequence Data; Nuclear Localization Signals / genetics; Photochemical Processes; Protein Engineering / methods; Synthetic Biology
TL;DR:
Since the light-activated NLS is genetically encoded and optically triggered, it will prove useful to address a variety of problems requiring spatial and temporal control of protein function, for example, in stem-cell, developmental, and cancer biology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Genetically Encoded Optochemical Probes for Simultaneous Fluorescence Reporting and Light Activation of Protein Function with Two-Photon Excitation
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 136(44), 15551–15558.
MeSH headings : Chromatography, Liquid; Fluorescence; HEK293 Cells; Humans; Methanosarcina barkeri / chemistry; Molecular Probes; Photons; Proteins / chemistry; Proteins / physiology; Tandem Mass Spectrometry
TL;DR:
The small size of coumarin, the site-specific incorporation, the application as both a light-activated caging group and as a fluorescent probe, and the broad range of excitation wavelengths are advantageous over other genetically encoded photocontrol systems and provide a precise and multifunctional tool for cellular biology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2014 journal article
Synthesis of Non-linear Protein Dimers through a Genetically Encoded Thiol-ene Reaction
PLOS ONE, 9(9).
MeSH headings : Alkenes / metabolism; Animals; Fluorescent Dyes / metabolism; Green Fluorescent Proteins / metabolism; Lysine / metabolism; Methanosarcina barkeri / metabolism; Muramidase / metabolism; Myoglobin / metabolism; Protein Multimerization; Sulfhydryl Compounds / metabolism
TL;DR:
The genetic encoding of a set of alkene lysines is demonstrated using the orthogonal PylRS/PylTCUA pair in Escherichia coli, showing the applicability of this approach in the formation of heterogeneous non-linear fused proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2013 journal article
Genetically encoded light-activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells
Journal of the American Chemical Society, 135(36), 13433–13439.
MeSH headings : Activating Transcription Factors / genetics; Activating Transcription Factors / metabolism; DNA-Directed RNA Polymerases / genetics; DNA-Directed RNA Polymerases / metabolism; Gene Expression Regulation, Enzymologic / genetics; Gene Silencing; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; HEK293 Cells; Humans; Light; Luciferases / genetics; Luciferases / metabolism; Models, Molecular; Molecular Structure; Photochemical Processes; Protein Engineering; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
The photocaging of a synthetic gene network using unnatural amino acid mutagenesis in mammalian cells was demonstrated with an engineered bacteriophage RNA polymerase, demonstrating tight spatial and temporal control.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: NC State University Libraries
Added: August 6, 2018
2012 journal article
Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction
NATURE CHEMISTRY, 4(4), 298–304.
MeSH headings : Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / metabolism; Cell Line; Fluorescent Dyes / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Humans; Lysine / analogs & derivatives; Lysine / metabolism; Norbornanes / chemistry; Proteins / genetics; Proteins / metabolism; Rhodamines / chemistry
TL;DR:
The genetic encoding of a norbornene amino acid using the pyrrolysyl tRNA synthetase/tRNA(CUA) pair in Escherichia coli and mammalian cells and the rapid bioorthogonal site-specific labelling of a protein on the mammalian cell surface is demonstrated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2011 journal article
Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 50(30), 6839–6842.
author keywords: caged compounds; gene technology; light-activation; mutagenesis; zinc-finger nucleases
MeSH headings : Endonucleases / genetics; Endonucleases / metabolism; Escherichia coli / genetics; HEK293 Cells; Humans; Models, Molecular; Mutagenesis; Photochemical Processes; Protein Engineering; Tyrosine / genetics; Ultraviolet Rays; Zinc Fingers
TL;DR:
A family of artificial restriction enzymes, namely zinc-finger nucleases (ZFN), has been developed to sequence-selectively achieve dsDNA scission and have since emerged as important and widely recognized tools for the genetic modification of cells, model organisms, and possibly humans to investigate gene function and to treat genetic disorders.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Genetically encoding an aliphatic diazirine for protein photocrosslinking
CHEMICAL SCIENCE, 2(3), 480–483.
TL;DR:
A novel aliphatic diazirine amino acid and its genetically encoded, site-specific incorporation into proteins in bacterial and mammalian cells are reported and efficient photocrosslinking of a test protein is demonstrated in vitro and in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2010 journal article
Photocaged T7 RNA Polymerase for the Light Activation of Transcription and Gene Function in Pro- and Eukaryotic Cells
CHEMBIOCHEM, 11(7), 972–977.
author keywords: amino acids; caged proteins; light activation; polymerases; RNA
MeSH headings : Catalytic Domain; Cell Line; DNA-Directed RNA Polymerases / genetics; DNA-Directed RNA Polymerases / metabolism; Escherichia coli / enzymology; Humans; Luciferases / genetics; Luciferases / metabolism; Models, Molecular; Photochemical Processes; Transcriptional Activation; Tyrosine / chemistry; Ultraviolet Rays; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
The ability to achieve spatiotemporal regulation of orthogonal RNA synthesis enables the precise dissection and manipulation of a wide range of cellular events, including gene function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
A Light-Activated DNA Polymerase
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 48(32), 5950–5953.
author keywords: DNA polymerases; mutagenesis; non-natural amino acids; photoactivation; polymerase chain reaction
MeSH headings : Catalytic Domain; Mutation; Nitrobenzenes / chemistry; Photolysis; Polymerase Chain Reaction; Taq Polymerase / chemistry; Taq Polymerase / metabolism; Tyrosine / chemistry; Ultraviolet Rays
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Plant cell calcium-rich environment enhances thermostability of recombinantly produced α-amylase from the hyperthermophilic bacterium Thermotoga maritime
Biotechnology and Bioengineering, 104(5), 947–956.
author keywords: hyperthermophilic enzymes; starch processing; transgenic plants; tobacco NT1 Cell cultures; biofuel
MeSH headings : Calcium / pharmacology; Coenzymes / pharmacology; Enzyme Stability; Escherichia coli / enzymology; Escherichia coli / genetics; Hot Temperature; Plants, Genetically Modified / enzymology; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Tobacco / enzymology; Tobacco / genetics; alpha-Amylases / chemistry; alpha-Amylases / genetics; alpha-Amylases / metabolism
TL;DR:
In a proof of concept study, tobacco cell cultures were used as model system to test in planta production of a hyperthermophilic α‐amylase from Thermotoga maritima, demonstrating the importance of a calcium‐rich environment for thermostability, as well as the advantage of producing this enzyme directly in plant cells where calcium is readily available.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018
2009 journal article
Temperature, not LuxS, mediates AI-2 formation in hydrothermal habitats
FEMS MICROBIOLOGY ECOLOGY, 68(2), 173–181.
author keywords: autoinducer-2; AI-2; hyperthermophiles; hydrothermal environments; Thermotoga maritima; Pyrococcus furiosus
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carbon-Sulfur Lyases / genetics; Carbon-Sulfur Lyases / metabolism; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation, Bacterial; Homoserine / analogs & derivatives; Homoserine / biosynthesis; Hot Temperature; Lactones; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Quorum Sensing; Thermotoga maritima / genetics; Thermotoga maritima / metabolism
TL;DR:
Autoinducer-2 (AI-2), a boronated furanone and proposed 'universal' interspecies mesophilic bacterial communication signal, could be formed by Thermotoga maritima and Pyrococcus furiosus through a combination of biotic and abiotic reaction steps, but did not induce any detectable quorum-sensing phenotypes in these organisms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2008 review
Hydrogenesis in hyperthermophilic microorganisms: Implications for biofuels
[Review of ]. METABOLIC ENGINEERING, 10(6), 394–404.
author keywords: Biohydrogen; Pyrococcus furiosus; Thermotoga maritima; Caldicellulosiruptor saccharolyticus; Hyperthermophiles; Hydrogenases
MeSH headings : Archaea / metabolism; Bioelectric Energy Sources; Hot Temperature; Hydrogen / metabolism; Hydrogen Sulfide / metabolism; Methane / metabolism
TL;DR:
To fully exploit the use of these novel microorganisms and their constituent hydrogenases for biohydrogen production, development of versatile genetic systems and improvements in current understanding of electron flux from fermentable substrates to H(2) in hyperthermophiles are needed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 journal article
Functional-genomics-based identification and characterization of open reading frames encoding alpha-glucoside-processing enzymes in the hyperthermophilic archaeon Pyrococcus furiosus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74(4), 1281–1283.
MeSH headings : Computational Biology; Gene Expression Regulation, Bacterial / genetics; Genomics / methods; Open Reading Frames / genetics; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; alpha-Glucosidases / genetics; beta-Amylase / genetics
TL;DR:
Bioinformatics analysis and transcriptional response information for Pyrococcus furiosus led to the identification of a novel isomaltase representing a new glycoside hydrolase family, a novel GH57 β-amylase, and an extracellular starch-binding protein.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 journal article
Impact of substrate glycoside linkage. and elemental sulfur on bioenergetics, of and hydrogen production by the hyperthermophilic Archaeon Pyrococcus furiosus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(21), 6842–6853.
MeSH headings : Archaea; Chemical Phenomena; Chemistry; Gene Expression Regulation, Archaeal; Genome, Archaeal; Glycosides / metabolism; Hot Temperature; Hydrogen / metabolism; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / physiology; Sulfur / metabolism
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2007 journal article
Responses of wild-type and resistant strains of the hyperthermophilic bacterium Thermotoga maritima to chloramphenicol challenge
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(15), 5058–5065.
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Chloramphenicol / pharmacology; Drug Resistance, Bacterial / genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hot Temperature; Microbial Sensitivity Tests; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Point Mutation; Proteome; RNA, Ribosomal, 23S / genetics; Thermotoga maritima / drug effects; Thermotoga maritima / genetics; Thermotoga maritima / growth & development; Transcription, Genetic
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2006 journal article
Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus
JOURNAL OF BACTERIOLOGY, 188(6), 2115–2125.
MeSH headings : Archaeal Proteins / genetics; Bacterial Proteins / genetics; DNA, Archaeal / chemistry; DNA, Archaeal / genetics; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Glucose / metabolism; Glucosephosphates / metabolism; Glucosyltransferases / genetics; Glycogen Debranching Enzyme System / genetics; Glycoside Hydrolases / genetics; Monosaccharide Transport Proteins / genetics; Oligonucleotide Array Sequence Analysis; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Sequence Analysis, DNA; Starch / metabolism; Substrate Specificity
TL;DR:
Starch metabolism in P. furiosus was examined by biochemical analyses in conjunction with global transcriptional response data for cells grown on a variety of glucans, and several hypothetical proteins encoded by three gene clusters are also involved in the transport and processing of alpha-glucan substrates by this organism.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
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