@article{tarigo_linder_neel_harvey_remick_grindem_2006, title={What is your diagnosis? Reluctant to dive: coelomic effusion in a frog}, volume={35}, ISSN={["0275-6382"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-33750693063&partnerID=MN8TOARS}, DOI={10.1111/j.1939-165X.2006.tb00145.x}, abstractNote={Abstract An adult female, albino South African Clawed frog (Xenopus laevis) from a research colony at the Biological Resources Facility of the College of Agriculture and Life Sciences at North Carolina State University (NCSU) was presented with depression, lethargy, loss of diving reflex, and a distended abdomen. Cytologic examination of coelomic effusion fluid at the NCSU veterinary teaching hospital revealed a mixed population of inflammatory cells, including heterophils and a predominance of large mononuclear cells (macrophages) that often contained intracytoplasmic, negatively‐stained, rod‐shaped to filamentous organisms consistent with Mycobacterium sp. Ziehl‐Neelsen stain revealed bright pink to red, acid‐fast organisms with a beaded appearance. Histopathologic findings in tissues obtained at necropsy included marked, multifocal to coalescing, heterophilic, granulomatous and fibrinous coelomitis as well as severe multifocal heterophilic and granulomatous hepatitis, interstitial pneumonia and sinusitis/rhinitis. Slender gram‐positive, acid‐fast bacterial rods were identified in sections of coelomic pleura, kidneys, nasal cavities, spleen, liver, and pulmonary interstitium, indicative of systemic mycobacteriosis. Based on mycobacterial culture, the organism was identified as M marinum complex. Mycobacteria are variably gram‐positive, often acid‐fast, small rods that are ubiquitous in aquatic environments. The clinical and pathologic spectrum of disease in amphibians depends on host and pathogen status. Xenopus sp and several other frogs are good models for studying the pathogenesis of M tuberculosis infection. In addition to culture, polymerase chain reaction assays may be used for definitive identification of the organisms; accurate speciation may require further genetic investigation.}, number={3}, journal={VETERINARY CLINICAL PATHOLOGY}, author={Tarigo, Jaime and Linder, Keith and Neel, Jennifer and Harvey, Stephen and Remick, Amera and Grindem, Carol}, year={2006}, month={Sep}, pages={341–344} }