Rachael E. Eckert

author keywords: inflammation; adhesion; beta 2 integrin; actin

MeSH headings : Actins / metabolism; CD18 Antigens / metabolism; Cell Adhesion / drug effects; Cell Membrane / drug effects; Cell Membrane / metabolism; Cell Polarity / drug effects; Chemotactic Factors / pharmacology; Chemotaxis, Leukocyte / drug effects; Humans; Intracellular Signaling Peptides and Proteins / metabolism; Membrane Proteins / metabolism; Myristoylated Alanine-Rich C Kinase Substrate; N-Formylmethionine Leucyl-Phenylalanine / pharmacology; Neutrophils / cytology; Neutrophils / drug effects; Neutrophils / metabolism; Peptides / pharmacology; Tetradecanoylphorbol Acetate / pharmacology

TL;DR: Treating isolated human neutrophils with MANS significantly inhibited both their migration and beta2 integrin-dependent adhesion in response to N-formyl-methionyl-leucyl-phenylalanine (fMLF), IL-8, or leukotriene (LT)B(4). (via Semantic Scholar)

author keywords: Endotoxemia; LPS; p38 MAPK; Equine; Leukocytes; Inflammation

MeSH headings : Animals; Cells, Cultured; Chemokines / genetics; Chemokines / metabolism; Cytokines / genetics; Cytokines / metabolism; Gene Expression Regulation / physiology; Horses / blood; Horses / immunology; Horses / metabolism; Leukocytes / drug effects; Leukocytes / metabolism; Lipopolysaccharides / pharmacology; RNA, Messenger / metabolism; Signal Transduction / drug effects; Signal Transduction / physiology; p38 Mitogen-Activated Protein Kinases / metabolism

TL;DR: TNFalpha,IL-1beta, IL-6 and IL-8 are induced upon LPS stimulation of equine leukocytes and that this induction of gene expression is dependent on the p38 MAPK pathway, however, there are differences in the efficacy of the p 38 inhibitors tested here that may be explained by differences in specificity or potency. (via Semantic Scholar)

author keywords: Other animals; Inflammation; Laminitis

MeSH headings : Animals; CD18 Antigens / metabolism; Cell Adhesion; Cells, Cultured; Chemotaxis, Leukocyte / physiology; Dose-Response Relationship, Drug; Enzyme Inhibitors / pharmacology; Gene Expression Regulation / physiology; Horses / physiology; Imidazoles / pharmacology; Inflammation / metabolism; Leukotriene B4 / metabolism; Neutrophils / metabolism; Pyridines / pharmacology; p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases / metabolism

TL;DR: Findings support a function for p38 MAPK in equine neutrophil migration and suggest the potential for the ability of p38MAPK inhibition to limit neutrophilic inflammation in the laminae during acute laminitis. (via Semantic Scholar)

author keywords: PK4; chemotaxis; fMLF

MeSH headings : Actins / metabolism; Blotting, Western; Calcium-Calmodulin-Dependent Protein Kinase Type 2 / antagonists & inhibitors; Calcium-Calmodulin-Dependent Protein Kinase Type 2 / physiology; Cell Adhesion; Cell Adhesion Molecules / genetics; Cell Adhesion Molecules / metabolism; Cells, Cultured; Chemotaxis; Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors; Cyclic AMP-Dependent Protein Kinases / physiology; Cyclic GMP / analogs & derivatives; Cyclic GMP / pharmacology; Cyclic GMP-Dependent Protein Kinases / antagonists & inhibitors; Cyclic GMP-Dependent Protein Kinases / physiology; Enzyme Inhibitors / pharmacology; Humans; Interleukin-8 / pharmacology; Leukotriene B4 / pharmacology; Microfilament Proteins / genetics; Microfilament Proteins / metabolism; N-Formylmethionine Leucyl-Phenylalanine / analogs & derivatives; N-Formylmethionine Leucyl-Phenylalanine / pharmacology; Neutrophils / drug effects; Neutrophils / metabolism; Phosphoproteins / genetics; Phosphoproteins / metabolism; Phosphorylation / drug effects; Platelet Activating Factor / pharmacology; Protein Kinase C / antagonists & inhibitors; Protein Kinase C / physiology; Serine / chemistry; Serine / genetics; Serine / metabolism; Signal Transduction; Tumor Necrosis Factor-alpha / pharmacology

TL;DR: The data show that chemoattractant stimulation of human neutrophils induces a rapid and transient PKA‐dependent VASP Ser 157 phosphorylation, andhesion does not appear to be an important regulator of the state of Vasodilator‐stimulated phosphoprotein. (via Semantic Scholar)

author keywords: other animals; endotoxin shock; lipopolysaccharide; inflammation

MeSH headings : Animals; Cells, Cultured; Cyclooxygenase 2 / genetics; Cyclooxygenase 2 / metabolism; Enzyme Activation; Epoprostenol / metabolism; Gene Expression Regulation, Enzymologic; Horses / blood; Horses / genetics; Imidazoles / pharmacology; Leukocytes / metabolism; Prostaglandins / genetics; Prostaglandins / metabolism; Pyridines / pharmacology; RNA, Messenger / metabolism; Time Factors; p38 Mitogen-Activated Protein Kinases / metabolism

TL;DR: It is concluded that p 38 MAPK is essential for LPS-induced COX-2 expression suggesting that p38 MAPK are a potential target for anti-inflammatory therapy during equine endotoxemia. (via Semantic Scholar)