Raghunath B. Shivappa

Works (5)

Updated: July 5th, 2023 15:54

2009 article

Immunostimulatory effects of natural human interferon-alpha (huIFN-α) on carps Cyprinus carpio L.

Watanuki, H., Chakraborty, G., Korenaga, H., Kono, T., Shivappa, R. B., & Sakai, M. (2009, April 20). Veterinary Immunology and Immunopathology.

By: H. Watanuki*, G. Chakraborty *, H. Korenaga*, T. Kono*, R. Shivappa n & M. Sakai *

author keywords: huIFN-alpha; Phagocytic activity; Cytokine genes; Fish
MeSH headings : Adjuvants, Immunologic / administration & dosage; Adjuvants, Immunologic / pharmacology; Administration, Oral; Animals; Base Sequence; Carps / genetics; Carps / immunology; Carps / physiology; Cytokines / genetics; DNA Primers / genetics; Gene Expression / drug effects; Humans; Interferon-alpha / administration & dosage; Interferon-alpha / pharmacology; Interleukin-10 / genetics; Interleukin-1beta / genetics; Kidney / drug effects; Kidney / immunology; Kidney / physiology; Phagocytes / drug effects; Phagocytes / immunology; Phagocytes / physiology; Phagocytosis; Superoxides / metabolism; Tumor Necrosis Factor-alpha / genetics
topics (OpenAlex): Aquaculture disease management and microbiota; interferon and immune responses; Immune Response and Inflammation
TL;DR: The results indicate that a low dose of huIFN-alpha significantly increased phagocytic activity and superoxide anion production in the carp kidney and the huIPA-treated fish displayed a significant upregulation in cytokine gene expression. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2008 article

Detection of Nucleospora salmonis in cutthroat trout (Oncorhynchus clarki) and rainbow trout (Oncorhynchus mykiss) by loop-mediated isothermal amplification

Sakai, M., Baxa, D. V., Kurobe, T., Kono, T., Shivappa, R. B., & Hedrick, R. P. (2008, November 14). Aquaculture.

By: M. Sakai *, D. Baxa *, T. Kurobe *, T. Kono*, R. Shivappa n & R. Hedrick*

author keywords: Nucleospora salmonis; Detection; PCR; LAMP; Rainbow trout
topics (OpenAlex): Aquaculture disease management and microbiota; Biosensors and Analytical Detection; Identification and Quantification in Food
TL;DR: A highly sensitive and rapid diagnostic procedure for detection of N. salmonis infections in cutthroat trout and rainbow trout and the detection-limit using LAMP was found to be similar to polymerase chain reaction (PCR). (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2008 journal article

Detection of spring viraemia of carp virus (SVCV) by loop-mediated isothermal amplification (LAMP) in koi carp, Cyprinus carpio L

Journal of Fish Diseases, 31(4), 249–258.

By: R. Shivappa n, R. Savan *, T. Kono*, M. Sakai *, E. Emmenegger *, G. Kurath *, J. Levine n 

author keywords: detection; koi carp; loop mediated isothermal amplification; spring viraemia of carp virus
MeSH headings : Animals; Base Sequence; Carps / virology; Cell Line, Tumor; DNA Primers / chemistry; Fish Diseases / diagnosis; Fish Diseases / virology; Nucleic Acid Amplification Techniques / veterinary; Rhabdoviridae Infections / diagnosis; Rhabdoviridae Infections / veterinary; Sensitivity and Specificity; Vesiculovirus / genetics; Vesiculovirus / isolation & purification; Viral Envelope Proteins / genetics
topics (OpenAlex): Aquaculture disease management and microbiota; Animal Virus Infections Studies; Mosquito-borne diseases and control
TL;DR: The isothermal temperature requirements, high specificity and sensitivity, and short incubation time of the RT-LAMP assay make it an excellent choice as a field diagnostic test for SVCV. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2007 article

Challenges in Quantification of Ligninolytic Enzymes from Phanerochaete chrysosporium Cultivation for Pretreatment of Cotton Stalks

Shi, N. J., Sharma-Shivappa, N. R. R., Chinn, N. M., Dean, N. R. A., & Shivappa, N. R. B. (2007, January 1). Transactions of the ASABE, Vol. 50, pp. 2347–2354.

By: N. Shi, N. Sharma-Shivappa*, N. Chinn* , N. Dean*  & N. Shivappa*

topics (OpenAlex): Enzyme-mediated dye degradation; Fungal Biology and Applications; Microbial Metabolism and Applications
Sources: NC State University Libraries, NC State University Libraries
Added: August 6, 2018

2007 article

Phylogenetic analysis of spring virema of carp virus reveals distinct subgroups with common origins for recent isolates in North America and the UK

Miller, O., Fuller, F. J., Gebreyes, W. A., Lewbart, G. A., Shchelkunov, I. S., Shivappa, R. B., … Levine, J. F. (2007, July 16). Diseases of Aquatic Organisms, Vol. 76, pp. 193–204.

By: O. Miller n, F. Fuller n , W. Gebreyes n, G. Lewbart n , I. Shchelkunov, R. Shivappa n, C. Joiner *, G. Woolford* ...

author keywords: spring viremia of carp; SVCV; phosphoprotein gene; glycoprotein gene; phylogenetic analysis; molecular epidemiology
MeSH headings : Animals; Base Sequence; Carps; Fish Diseases / virology; Genetic Variation; Glycoproteins / chemistry; Glycoproteins / genetics; Molecular Sequence Data; North America; Phosphoproteins / chemistry; Phosphoproteins / genetics; Phylogeny; RNA, Viral / chemistry; RNA, Viral / genetics; Reverse Transcriptase Polymerase Chain Reaction / veterinary; Rhabdoviridae / classification; Rhabdoviridae / genetics; Rhabdoviridae / isolation & purification; Rhabdoviridae Infections / veterinary; Rhabdoviridae Infections / virology; Sequence Alignment; Sequence Analysis, DNA; United Kingdom
topics (OpenAlex): Aquaculture disease management and microbiota; Microbial infections and disease research; Bacteriophages and microbial interactions
TL;DR: Combined P- and G-sequence data support the emergence of SVCV in Illinois,USA, and in Lake Ontario, Canada, from the initial outbreak in Wisconsin, USA, and demonstrate a close genetic link to viruses isolated during routine import checks on fish brought into the UK from Asia. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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