Helen Rawsthorne

Works (8)

Updated: July 5th, 2023 15:54

2014 article

Molecular methods used to estimate thermal inactivation of a prototype human norovirus: More heat resistant than previously believed?

Escudero-Abarca, B. I., Rawsthorne, H., Goulter, R. M., Suh, S. H., & Jaykus, L. A. (2014, January 30). Food Microbiology.

By: B. Escudero-Abarca n, H. Rawsthorne n, R. Goulter*, S. Suh n & L. Jaykus n

author keywords: Human norovirus; Thermal inactivation; Heat resistance
MeSH headings : Azides / chemistry; Caliciviridae Infections / virology; Hot Temperature; Humans; Norovirus / chemistry; Norovirus / genetics; Norovirus / isolation & purification; Norovirus / physiology; Polymerase Chain Reaction; Propidium / analogs & derivatives; Propidium / chemistry; RNA, Viral / chemistry; RNA, Viral / genetics; Virus Inactivation
topics (OpenAlex): Viral gastroenteritis research and epidemiology; Viral Infections and Immunology Research; Animal Virus Infections Studies
TL;DR: Electron microscopy data applied to heat-treated virus-like particles supported this relatively high degree of thermal resistance and suggest that SMV is more heat resistant than common cultivable HuNoV surrogates. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 article

Persistence and Transferability of Noroviruses on and between Common Surfaces and Foods

Escudero, B. I., Rawsthorne, H., Gensel, C., & Jaykus, L. A. (2012, May 1). Journal of Food Protection.

By: B. Escudero n, H. Rawsthorne n, C. Gensel n & L. Jaykus n

MeSH headings : Colony Count, Microbial; Consumer Product Safety; Equipment Contamination; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Humans; Hygiene; Norovirus / growth & development; Reverse Transcriptase Polymerase Chain Reaction; Risk Assessment; Time Factors; Viral Plaque Assay
topics (OpenAlex): Viral gastroenteritis research and epidemiology; Infection Control and Ventilation
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Source: Web Of Science
Added: August 6, 2018

2011 article

Detection and enumeration of Dekkera anomala in beer, cola, and cider using real-time PCR

Gray, S. R., Rawsthorne, H., Dirks, B., & Phister, T. G. (2011, January 18). Letters in Applied Microbiology.

By: S. Gray n, H. Rawsthorne n, B. Dirks * & T. Phister n

author keywords: Dekkera anomala; QPCR; real-time PCR; spoilage yeast
MeSH headings : Base Sequence; Beer / microbiology; Beverages / microbiology; DNA Primers / chemistry; Dekkera / growth & development; Dekkera / isolation & purification; Food Microbiology; Malus; Molecular Sequence Data; Polymerase Chain Reaction / methods
topics (OpenAlex): Fermentation and Sensory Analysis; Yeasts and Rust Fungi Studies; Plant Pathogens and Fungal Diseases
TL;DR: A quantitative real‐time PCR assay for detection and enumeration of the spoilage yeast Dekkera anomala in beer, cola, apple cider, and brewing wort is presented as an improvement upon existing detection methods, which are very time‐consuming and not always accurate. (via Semantic Scholar)
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13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2009 article

Detection of viable Zygosaccharomyces bailii in fruit juices using ethidium monoazide bromide and real-time PCR

Rawsthorne, H., & Phister, T. G. (2009, February 5). International Journal of Food Microbiology.

By: H. Rawsthorne n & T. Phister n

author keywords: Zygosaccharomyces bailii; Fruit juice; Real-time PCR; Viable-nonculturable; Ethidium monoazide
MeSH headings : Azides; Beverages / microbiology; Colony Count, Microbial; DNA, Bacterial / analysis; Ethanol; Fluorescent Dyes; Food Microbiology; Fruit / microbiology; Hot Temperature; Microbial Viability; Microscopy, Fluorescence; Reverse Transcriptase Polymerase Chain Reaction; Rosaceae / microbiology; Vaccinium macrocarpon / microbiology; Zygosaccharomyces / drug effects; Zygosaccharomyces / genetics; Zygosaccharomyces / isolation & purification
topics (OpenAlex): Identification and Quantification in Food; Molecular Biology Techniques and Applications; Mycotoxins in Agriculture and Food
TL;DR: An assay which will be useful for the detection of viable spoilage yeasts in various fruit juices is designed, which could detect as few as 12.5 viable Z. bailii cells in the presence of 10(5) CFU/ml of heat killed-cells. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2009 article

Development of a Fluorescent In Situ Method for Visualization of Enteric Viruses

Rawsthorne, H., Phister, T. G., & Jaykus, L.-A. (2009, October 24). Applied and Environmental Microbiology.

By: H. Rawsthorne n, T. Phister n & L. Jaykus n

MeSH headings : Animals; Biotin; Cell Line; Hepatitis A virus / isolation & purification; Hepatitis A virus / ultrastructure; Humans; Indicators and Reagents; Mice; Microscopy, Confocal / methods; Microscopy, Fluorescence / methods; Norovirus / isolation & purification; Norovirus / ultrastructure; Onions / virology; Quantum Dots; Streptavidin
topics (OpenAlex): Viral gastroenteritis research and epidemiology; Cytomegalovirus and herpesvirus research; Viral Infections and Immunology Research
TL;DR: The biotinylation process did not impact virus infectivity, nor did it interfere with the interactions between the virus and host cells or model produce items, and the method provides a powerful tool for the labeling and detection of enteric viruses (and their surrogates) which can be used to track virus behavior in situ. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 article

PCR-Based Method Using Propidium Monoazide To Distinguish Viable from Nonviable Bacillus subtilis Spores

Rawsthorne, H., Dock, C. N., & Jaykus, L. A. (2009, March 7). Applied and Environmental Microbiology.

By: H. Rawsthorne n, C. Dock n & L. Jaykus n

MeSH headings : Azides / metabolism; Azides / pharmacology; Bacillus subtilis / genetics; Bacillus subtilis / metabolism; Bacillus subtilis / physiology; DNA / metabolism; Fluorescent Dyes / metabolism; Fluorescent Dyes / pharmacology; Microbial Viability; Polymerase Chain Reaction / methods; Propidium / analogs & derivatives; Propidium / metabolism; Propidium / pharmacology; Spores / genetics; Spores / metabolism; Spores / physiology
topics (OpenAlex): Animal Genetics and Reproduction; Bacteriophages and microbial interactions; Bacterial Genetics and Biotechnology
TL;DR: A molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide is described. (via Semantic Scholar)
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10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 article

The presence ofSaccharomyces cerevisiaeDNA in various media used to propagate yeasts and its removal by ethidium monoazide

Rawsthorne, H., & Phister, T. G. (2009, July 31). Letters in Applied Microbiology.

By: H. Rawsthorne* & T. Phister*

author keywords: contamination; ethidium monoazide; real-time PCR; Saccharomyces cerevisiae
MeSH headings : Azides / chemistry; Culture Media / chemistry; DNA, Fungal / genetics; DNA, Fungal / isolation & purification; Polymerase Chain Reaction; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / isolation & purification; Yeasts / growth & development
topics (OpenAlex): Fermentation and Sensory Analysis; Identification and Quantification in Food; Food Quality and Safety Studies
TL;DR: This study demonstrates the interference of yeast extract in enumeration of Saccharomyces cerevisiae using real‐time PCR and develops a method for its removal from the media using ethidium monoazide (EMA). (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2007 article

Characterization of the lactococcal group II intron target site in its native host

Sela, D. A., Rawsthorne, H., & Mills, D. A. (2007, April 6). Plasmid.

By: D. Sela *, H. Rawsthorne* & D. Mills*

author keywords: Lactococcus lactis; Ll.ltrB; group II intron; QPCR; lactic acid bacteria; real-time PCR
MeSH headings : Bacterial Proteins / genetics; Base Sequence; Cloning, Molecular; DNA Transposable Elements / genetics; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Enterococcus faecalis / genetics; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Introns / genetics; Lactococcus lactis / genetics; Models, Biological; Models, Genetic; Molecular Sequence Data; Plasmids / genetics; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity
topics (OpenAlex): Probiotics and Fermented Foods; Enterobacteriaceae and Cronobacter Research; Carbohydrate Chemistry and Synthesis
TL;DR: A quantitative real-time PCR (QPCR) assay was developed to test target site nucleotides previously demonstrated as critical for homing in E. coli, for relevance in its native host. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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