Helen Rawsthorne

Works (8)

Updated: July 5th, 2023 15:54

2014 journal article

Molecular methods used to estimate thermal inactivation of a prototype human norovirus: More heat resistant than previously believed?

FOOD MICROBIOLOGY, 41, 91–95.

By: B. Escudero-Abarca n, H. Rawsthorne n, R. Goulter n, S. Suh n & L. Jaykus n

author keywords: Human norovirus; Thermal inactivation; Heat resistance
MeSH headings : Azides / chemistry; Caliciviridae Infections / virology; Hot Temperature; Humans; Norovirus / chemistry; Norovirus / genetics; Norovirus / isolation & purification; Norovirus / physiology; Polymerase Chain Reaction; Propidium / analogs & derivatives; Propidium / chemistry; RNA, Viral / chemistry; RNA, Viral / genetics; Virus Inactivation
TL;DR: Electron microscopy data applied to heat-treated virus-like particles supported this relatively high degree of thermal resistance and suggest that SMV is more heat resistant than common cultivable HuNoV surrogates. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 journal article

Persistence and Transferability of Noroviruses on and between Common Surfaces and Foods

JOURNAL OF FOOD PROTECTION, 75(5), 927–935.

By: B. Escudero n, H. Rawsthorne n, C. Gensel n & L. Jaykus n

MeSH headings : Colony Count, Microbial; Consumer Product Safety; Equipment Contamination; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Humans; Hygiene; Norovirus / growth & development; Reverse Transcriptase Polymerase Chain Reaction; Risk Assessment; Time Factors; Viral Plaque Assay
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Source: Web Of Science
Added: August 6, 2018

2011 journal article

Detection and enumeration of Dekkera anomala in beer, cola, and cider using real-time PCR

LETTERS IN APPLIED MICROBIOLOGY, 52(4), 352–359.

By: S. Gray n, H. Rawsthorne n, B. Dirks* & T. Phister n

author keywords: Dekkera anomala; QPCR; real-time PCR; spoilage yeast
MeSH headings : Base Sequence; Beer / microbiology; Beverages / microbiology; DNA Primers / chemistry; Dekkera / growth & development; Dekkera / isolation & purification; Food Microbiology; Malus; Molecular Sequence Data; Polymerase Chain Reaction / methods
TL;DR: A quantitative real‐time PCR assay for detection and enumeration of the spoilage yeast Dekkera anomala in beer, cola, apple cider, and brewing wort is presented as an improvement upon existing detection methods, which are very time‐consuming and not always accurate. (via Semantic Scholar)
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13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Detection of viable Zygosaccharomyces bailii in fruit juices using ethidium monoazide bromide and real-time PCR

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 131(2-3), 246–250.

By: H. Rawsthorne n & T. Phister n

author keywords: Zygosaccharomyces bailii; Fruit juice; Real-time PCR; Viable-nonculturable; Ethidium monoazide
MeSH headings : Azides; Beverages / microbiology; Colony Count, Microbial; DNA, Bacterial / analysis; Ethanol; Fluorescent Dyes; Food Microbiology; Fruit / microbiology; Hot Temperature; Microbial Viability; Microscopy, Fluorescence; Reverse Transcriptase Polymerase Chain Reaction; Rosaceae / microbiology; Vaccinium macrocarpon / microbiology; Zygosaccharomyces / drug effects; Zygosaccharomyces / genetics; Zygosaccharomyces / isolation & purification
TL;DR: An assay which will be useful for the detection of viable spoilage yeasts in various fruit juices is designed, which could detect as few as 12.5 viable Z. bailii cells in the presence of 10(5) CFU/ml of heat killed-cells. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2009 journal article

Development of a Fluorescent In Situ Method for Visualization of Enteric Viruses

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(24), 7822–7827.

By: H. Rawsthorne n, T. Phister n & L. Jaykus n

MeSH headings : Animals; Biotin; Cell Line; Hepatitis A virus / isolation & purification; Hepatitis A virus / ultrastructure; Humans; Indicators and Reagents; Mice; Microscopy, Confocal / methods; Microscopy, Fluorescence / methods; Norovirus / isolation & purification; Norovirus / ultrastructure; Onions / virology; Quantum Dots; Streptavidin
TL;DR: The biotinylation process did not impact virus infectivity, nor did it interfere with the interactions between the virus and host cells or model produce items, and the method provides a powerful tool for the labeling and detection of enteric viruses (and their surrogates) which can be used to track virus behavior in situ. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

PCR-Based Method Using Propidium Monoazide To Distinguish Viable from Nonviable Bacillus subtilis Spores

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(9), 2936–2939.

By: H. Rawsthorne n, C. Dock n & L. Jaykus n

MeSH headings : Azides / metabolism; Azides / pharmacology; Bacillus subtilis / genetics; Bacillus subtilis / metabolism; Bacillus subtilis / physiology; DNA / metabolism; Fluorescent Dyes / metabolism; Fluorescent Dyes / pharmacology; Microbial Viability; Polymerase Chain Reaction / methods; Propidium / analogs & derivatives; Propidium / metabolism; Propidium / pharmacology; Spores / genetics; Spores / metabolism; Spores / physiology
TL;DR: A molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide is described. (via Semantic Scholar)
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10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

The presence of Saccharomyces cerevisiae DNA in various media used to propagate yeasts and its removal by ethidium monoazide

LETTERS IN APPLIED MICROBIOLOGY, 49(5), 652–654.

By: H. Rawsthorne* & T. Phister*

author keywords: contamination; ethidium monoazide; real-time PCR; Saccharomyces cerevisiae
MeSH headings : Azides / chemistry; Culture Media / chemistry; DNA, Fungal / genetics; DNA, Fungal / isolation & purification; Polymerase Chain Reaction; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / isolation & purification; Yeasts / growth & development
TL;DR: This study demonstrates the interference of yeast extract in enumeration of Saccharomyces cerevisiae using real‐time PCR and develops a method for its removal from the media using ethidium monoazide (EMA). (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2007 journal article

Characterization of the lactococcal group II intron target site in its native host

PLASMID, 58(2), 127–139.

By: D. Sela *, H. Rawsthorne* & D. Mills*

author keywords: Lactococcus lactis; Ll.ltrB; group II intron; QPCR; lactic acid bacteria; real-time PCR
MeSH headings : Bacterial Proteins / genetics; Base Sequence; Cloning, Molecular; DNA Transposable Elements / genetics; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; Enterococcus faecalis / genetics; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Introns / genetics; Lactococcus lactis / genetics; Models, Biological; Models, Genetic; Molecular Sequence Data; Plasmids / genetics; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity
TL;DR: A quantitative real-time PCR (QPCR) assay was developed to test target site nucleotides previously demonstrated as critical for homing in E. coli, for relevance in its native host. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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