Elizabeth S. Haas

author keywords: Archaea; archaebacteria; ribonuclease P; ribozyme; rnpB

MeSH headings : Base Sequence; Cells, Cultured; DNA Primers / chemistry; Endoribonucleases / chemistry; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli Proteins; Genes, Bacterial / genetics; Molecular Sequence Data; Nucleic Acid Conformation; Phylogeny; Plasmids; Polymerase Chain Reaction; RNA, Archaeal / classification; RNA, Archaeal / genetics; RNA, Bacterial / chemistry; RNA, Bacterial / isolation & purification; RNA, Catalytic / chemistry; RNA, Catalytic / isolation & purification; Ribonuclease P; Sequence Alignment; Sequence Analysis, DNA

TL;DR: A detailed comparative analysis of archaeal RNase P RNA structure and a comparison of the resulting structural information with that of the bacterial RNA reveals that the ArchaealRNase P RNAs are strikingly similar to those of Bacteria. (via Semantic Scholar)

MeSH headings : Archaea / enzymology; Archaea / genetics; Bacillus / enzymology; Bacillus / genetics; Base Sequence; Conserved Sequence; Endoribonucleases / chemistry; Endoribonucleases / genetics; Endoribonucleases / metabolism; Kinetics; Methanobacterium / enzymology; Methanobacterium / genetics; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Phylogeny; RNA, Archaeal / chemistry; RNA, Archaeal / genetics; RNA, Archaeal / metabolism; RNA, Bacterial / metabolism; RNA, Catalytic / chemistry; RNA, Catalytic / genetics; RNA, Catalytic / metabolism; Ribonuclease P

TL;DR: The properties of the archaeal RNase P RNAs (high ionic-strength requirement, low affinity for substrate, and catalytic reconstitution by bacterialRNase P protein) are similar to synthetic RNases that contain all of the catalytic core of the bacterial RNA but lack phylogenetically variable, stabilizing elements. (via Semantic Scholar)

MeSH headings : Bacteria / enzymology; Bacteria / genetics; Base Sequence; Conserved Sequence; Endoribonucleases / chemistry; Endoribonucleases / genetics; Evolution, Molecular; Genetic Variation; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Phylogeny; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Catalytic / chemistry; RNA, Catalytic / genetics; Ribonuclease P

TL;DR: In a remarkable case of convergent molecular evolution, most of the unusual structural elements of type B RNase P RNAs of the low G+C Gram-positive Bacteria have evolved independently in Thermomicrobium roseum, a member of the green non-sulfur Bacteria. (via Semantic Scholar)

MeSH headings : Animals; Base Sequence; Cell Nucleolus / metabolism; Chickens; Conserved Sequence; DNA Primers; Female; Humans; Mice; Molecular Sequence Data; Nucleic Acid Conformation; Oocytes / physiology; Polymerase Chain Reaction; RNA Precursors / metabolism; RNA, Small Nuclear / biosynthesis; RNA, Small Nuclear / chemistry; Rats; Sequence Homology, Nucleic Acid; Xenopus laevis; Zebrafish

TL;DR: Human and frog E1, E2 and E3 RNAs injected into the cytoplasm of frog oocytes migrated to the nucleus and specifically to the nucleolus, indicating that the nucleolar and nuclear localization signals of these snoRNAs reside within their evolutionarily conserved segments. (via Semantic Scholar)