Ahmed Abdelshafy Mahmoud Gomaa

Works (3)

Updated: July 5th, 2023 15:41

2016 journal article

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

MOLECULAR CELL, 62(1), 137–147.

By: R. Leenay n, K. Maksimchuk n, R. Slotkowski n, R. Agrawal n, A. Gomaa n, A. Briner n, R. Barrangou n , C. Beisel n

MeSH headings : Bacillus / chemistry; Bacillus / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Escherichia coli / chemistry; Escherichia coli / metabolism; Francisella / chemistry; Francisella / metabolism; High-Throughput Screening Assays / methods; Protein Structure, Tertiary; Streptococcus thermophilus / chemistry; Streptococcus thermophilus / metabolism
TL;DR: An in vivo, positive, and tunable screen termed PAM-SCANR (PAM screen achieved by NOT-gate repression) to elucidate functional PAMs as well as an interactive visualization scheme termed the PAM wheel to convey individual PAM sequences and their activities to accelerate ability to understand and exploit the multitude of CRISPR-Cas systems in nature. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 article

A CRISPR design for next-generation antimicrobials

GENOME BIOLOGY, Vol. 15.

By: C. Beisel n, A. Gomaa n & R. Barrangou n 

MeSH headings : Animals; Anti-Infective Agents / administration & dosage; CRISPR-Cas Systems; Drug Resistance, Microbial / genetics; Enterohemorrhagic Escherichia coli / genetics; Gene Targeting; RNA, Guide, Kinetoplastida / genetics; Ribonucleases / genetics
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 journal article

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

MOLECULAR CELL, 56(2), 333–339.

By: A. Briner n, P. Donohoue *, A. Gomaa n, K. Selle n, E. Slorach*, C. Nye*, R. Haurwitz*, C. Beisel n, A. May *, R. Barrangou n 

MeSH headings : Bacterial Proteins / chemistry; CRISPR-Associated Protein 9; CRISPR-Associated Proteins / chemistry; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; DNA / chemistry; DNA Cleavage; Endonucleases / chemistry; Genetic Engineering / methods; HEK293 Cells; Humans; Nucleic Acid Conformation; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / ultrastructure
TL;DR: Six conserved modules within native crRNA:tracrRNA duplexes and single guide RNAs that direct Cas9 endonuclease activity are identified and characterized and it is shown the bulge and nexus are necessary for DNA cleavage and the nexus and hairpins are instrumental in defining orthogonality between systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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