@article{pereira_oliveira_andrade_haverroth_cardoso_martins_2024, title={Linking water-use strategies with drought resistance across herbaceous crops}, volume={176}, ISSN={["1399-3054"]}, url={http://dx.doi.org/10.1111/ppl.14114}, DOI={10.1111/ppl.14114}, abstractNote={Woody plants minimize xylem embolism formation during drought essentially by closing stomata at higher water potentials and/or by increasing the xylem resistance to embolism. Both of these mechanisms result in a higher stomatal safety margin (SSM), which is the water potential difference between stomatal closure and embolism formation. Here, we investigated whether increasing SSM represents a drought resistance mechanism for herbaceous plants and how the different water‐use strategies impact their survival. For that, we exposed four herbaceous crops with contrasting drought resistance to severe water deficit to assess drought‐induced damage and mortality. Unlike woody species, SSM was not associated with plant survival for herbaceous crops. Soybean, which presented the largest SSM across the four crops (1.67 MPa), exhibited the earliest mortality of leaves and whole plants as well as the highest rate of plant mortality (100%) at the end of the drought period. Cowpea, with an SSM of 0.63 MPa, was the most drought‐resistant species, with the latest leaf damage and the highest plant survival (100%). The most effective traits ensuring survival in herbaceous crops under severe drought were those related to drought avoidance mechanisms such as (1) early stomatal closure, (2) very low residual transpiration post‐stomatal closure, and (3) high capacitance pre‐ and post‐turgor loss.}, number={1}, journal={PHYSIOLOGIA PLANTARUM}, author={Pereira, Talitha S. and Oliveira, Leonardo A. and Andrade, Moab T. and Haverroth, Eduardo J. and Cardoso, Amanda A. and Martins, Samuel C. V.}, year={2024}, month={Jan} }
 @article{haverroth_oliveira_andrade_taggart_mcadam_zsogon_thompson_martins_cardoso_2023, title={Abscisic acid acts essentially on stomata, not on the xylem, to improve drought resistance in tomato}, volume={8}, ISSN={["1365-3040"]}, url={http://dx.doi.org/10.1111/pce.14676}, DOI={10.1111/pce.14676}, abstractNote={Drought resistance is essential for plant production under water-limiting environments. Abscisic acid (ABA) plays a critical role in stomata but its impact on hydraulic function beyond the stomata is far less studied. We selected genotypes differing in their ability to accumulate ABA to investigate its role in drought-induced dysfunction. All genotypes exhibited similar leaf and stem embolism resistance regardless of differences in ABA levels. Their leaf hydraulic resistance was also similar. Differences were only observed between the two extreme genotypes: sitiens (sit; a strong ABA-deficient mutant) and sp12 (a transgenic line that constitutively overaccumulates ABA), where the water potential inducing 50% embolism was 0.25 MPa lower in sp12 than in sit. Maximum stomatal and minimum leaf conductances were considerably lower in plants with higher ABA (wild type [WT] and sp12) than in ABA-deficient mutants. Variations in gas exchange across genotypes were associated with ABA levels and differences in stomatal density and size. The lower water loss in plants with higher ABA meant that lethal water potentials associated with embolism occurred later during drought in sp12 plants, followed by WT, and then by the ABA-deficient mutants. Therefore, the primary pathway by which ABA enhances drought resistance is via declines in water loss, which delays dehydration and hydraulic dysfunction.}, journal={PLANT CELL AND ENVIRONMENT}, publisher={Wiley}, author={Haverroth, Eduardo J. and Oliveira, Leonardo A. and Andrade, Moab T. and Taggart, Matthew and McAdam, Scott A. M. and Zsogon, Agustin and Thompson, Andrew J. and Martins, Samuel C. V. and Cardoso, Amanda A.}, year={2023}, month={Aug} }
 @article{oliveira_souza_andrade_oliveira_gouvea_martins_ramalho_cardoso_damatta_2023, title={Carbon gain is coordinated with enhanced stomatal conductance and hydraulic architecture in coffee plants acclimated to elevated [CO<sub>2</sub>]: The interplay with irradiance supply}, volume={204}, ISSN={["1873-2690"]}, url={http://dx.doi.org/10.1016/j.plaphy.2023.108145}, DOI={10.1016/j.plaphy.2023.108145}, abstractNote={We recently demonstrated that, under elevated [CO2] (eCa), coffee (Coffea arabica L.) plants grown at high light (HL), but not at low light (LL), display higher stomatal conductance (gs) than at ambient [CO2] (aCa). We then hypothesized that the enhanced gs at eCa/HL, if sustained at the long-term, would lead to adjustments in hydraulic architecture. To test this hypothesis, potted plants of coffee were grown in open-top chambers for 12 months under HL or LL (ca. 9 or 1 mol photons m-2 day-1, respectively); these light treatments were combined with two [CO2] levels (ca. 437 or 705 μmol mol-1, respectively). Under eCa/HL, increased gs was closely accompanied by increases in branch and leaf hydraulic conductances, suggesting a coordinated response between liquid- and vapor-phase water flows throughout the plant. Still under HL, eCa also resulted in increased Huber value (sapwood area-to-total leaf area), sapwood area-to-stem diameter, and root mass-to-total leaf area, thus further improving the water supply to the leaves. Our results demonstrate that Ca is a central player in coffee physiology increasing carbon gain through a close association between stomatal function and an improved hydraulic architecture under HL conditions.}, journal={PLANT PHYSIOLOGY AND BIOCHEMISTRY}, publisher={Elsevier BV}, author={Oliveira, Ueliton S. and Souza, Antonio H. and Andrade, Moab T. and Oliveira, Leonardo A. and Gouvea, Debora G. and Martins, Samuel C. V. and Ramalho, Jose D. C. and Cardoso, Amanda A. and DaMatta, Fabio M.}, year={2023}, month={Nov} }
 @article{souza_oliveira_oliveira_carvalho_andrade_pereira_gomes_cardoso_ramalho_martins_et al._2023, title={Growth and Leaf Gas Exchange Upregulation by Elevated [CO2] Is Light Dependent in Coffee Plants}, volume={12}, ISSN={["2223-7747"]}, url={http://dx.doi.org/10.3390/plants12071479}, DOI={10.3390/plants12071479}, abstractNote={Coffee (Coffea arabica L.) plants have been assorted as highly suitable to growth at elevated [CO2] (eCa), although such suitability is hypothesized to decrease under severe shade. We herein examined how the combination of eCa and contrasting irradiance affects growth and photosynthetic performance. Coffee plants were grown in open-top chambers under relatively high light (HL) or low light (LL) (9 or 1 mol photons m−2 day−1, respectively), and aCa or eCa (437 or 705 μmol mol–1, respectively). Most traits were affected by light and CO2, and by their interaction. Relative to aCa, our main findings were (i) a greater stomatal conductance (gs) (only at HL) with decreased diffusive limitations to photosynthesis, (ii) greater gs during HL-to-LL transitions, whereas gs was unresponsive to the LL-to-HL transitions irrespective of [CO2], (iii) greater leaf nitrogen pools (only at HL) and higher photosynthetic nitrogen-use efficiency irrespective of light, (iv) lack of photosynthetic acclimation, and (v) greater biomass partitioning to roots and earlier branching. In summary, eCa improved plant growth and photosynthetic performance. Our novel and timely findings suggest that coffee plants are highly suited for a changing climate characterized by a progressive elevation of [CO2], especially if the light is nonlimiting.}, number={7}, journal={PLANTS-BASEL}, publisher={MDPI AG}, author={Souza, Antonio H. and Oliveira, Ueliton S. and Oliveira, Leonardo A. and Carvalho, Pablo H. N. and Andrade, Moab T. and Pereira, Talitha S. and Gomes, Carlos C. and Cardoso, Amanda A. and Ramalho, Jose D. C. and Martins, Samuel C. V. and et al.}, year={2023}, month={Apr} }
 @article{armstrong_larson_harper_webb_dohleman_araya_meade_feng_mukoye_levin_et al._2023, title={One hundred important questions facing plant science: an international perspective}, volume={238}, ISSN={["1469-8137"]}, url={http://dx.doi.org/10.1111/nph.18771}, DOI={10.1111/nph.18771}, abstractNote={Summary The ‘One Hundred Important Questions Facing Plant Science Research’ project aimed to capture a global snapshot of the current issues and future questions facing plant science. This revisiting builds on the original 2011 paper. Over 600 questions were collected from anyone interested in plants, which were reduced to a final list of 100 by four teams of global panellists. There was remarkable consensus on the most important topics between the global subpanels. We present the top 100 most important questions facing plant science in 2022, ranging from how plants can contribute to tackling climate change, to plant‐defence priming and epigenome plasticity. We also provide explanations of why each question is important. We demonstrate how focussing on climate change, community and protecting plant life has become increasingly important for plant science over the past 11 years. This revisiting illustrates the collaborative and international need for long‐term funding of plant science research, alongside the broad community‐driven efforts to actively ameliorate and halt climate change, while adapting to its consequences.}, number={2}, journal={NEW PHYTOLOGIST}, publisher={Wiley}, author={Armstrong, Emily May and Larson, Emily R. and Harper, Helen and Webb, Cerian R. and Dohleman, Frank and Araya, Yoseph and Meade, Claire and Feng, Xiangyan and Mukoye, Benard and Levin, Maureece J. and et al.}, year={2023}, month={Apr}, pages={470–481} }
 @article{reynolds_taggart_martin_lobaton_cardoso_daniele_bozkurt_2023, title={Rapid Drought Stress Detection in Plants Using Bioimpedance Measurements and Analysis}, url={http://dx.doi.org/10.1109/tafe.2023.3330583}, DOI={10.1109/tafe.2023.3330583}, abstractNote={Smart farming is the targeted use of phenotyping for the rapid, continuous, and accurate assessment of plant health in the field. Bioimpedance monitoring can play a role in smart farming as a phenotyping method, which is now accessible thanks to recent efforts to commoditize and miniaturize electronics. Here, we demonstrate that bioimpedance measurements reflect the physiological changes in live plant tissue with induced alterations in their environmental conditions. When plants were exposed to $-$1.0 MPa polyethylene glycol, to simulate drought conditions, the extracellular resistance was observed to increase prior to the intercellular resistance, where the low frequency bioimpedance measurements increased by 25% within one hour. Similar patterns were observed when drought stress was applied to the plants by water withholding, with a bioimpedance increase within a matter of a few hours. The bioimpedance measurements were also compared with leaf relative water content, imaging, and field transpirable soil water, which reinforced these findings. These preliminary results suggest that bioimpedance can function as a phenotyping tool for continuous and real time monitoring of plant stress to allow the development of strategies to prevent damage from environmental stresses such as drought.}, journal={IEEE Transactions on AgriFood Electronics}, author={Reynolds, James and Taggart, Matthew and Martin, Devon and Lobaton, Edgar and Cardoso, Amanda A. and Daniele, Michael and Bozkurt, Alper}, year={2023} }
 @article{ugalde_cardoso_2023, title={When roots talk to shoots about flooding}, volume={8}, ISSN={["1532-2548"]}, url={http://dx.doi.org/10.1093/plphys/kiad464}, DOI={10.1093/plphys/kiad464}, journal={PLANT PHYSIOLOGY}, publisher={Oxford University Press (OUP)}, author={Ugalde, Jose Manuel and Cardoso, Amanda A.}, year={2023}, month={Aug} }
 @article{puglielli_laanisto_gori_cardoso_2023, title={Woody plant adaptations to multiple abiotic stressors: Where are we?*}, volume={299}, ISSN={["1618-0585"]}, url={http://dx.doi.org/10.1016/j.flora.2023.152221}, DOI={10.1016/j.flora.2023.152221}, abstractNote={Interacting abiotic stresses exert a fundamental selective pressure on the adaptive syndromes of long-living organisms such as woody plants. However, general patterns and mechanisms describing woody plant adaptations to tolerate multiple abiotic stressors are yet to emerge. This hampers our ability to build predictive frameworks foreseeing species responses to stochastic changes in abiotic stress regimes due to climate change. With this Virtual Special Issue (VSI), we aimed to summarize what we know, and what we do not know, about woody plant adaptations to achieve tolerance to multiple abiotic limitations. To this end, we brought together studies exploring ecological or ecophysiological perspectives on woody plant adaptations to tolerate multiple abiotic stresses. Ecological studies suggest patterns associating trait trade-offs, climate, and biotic interactions with woody plants’ multi-stress tolerance. Ecophysiological studies point to traits and conceptual frameworks that might explain some processes underpinning woody plant multi-stress tolerance. Here, we first revised the definitions of stress and stress tolerance used in ecological and ecophysiological research, providing a nomenclature of tolerance that could be used to unify definitions across research fields. Then, we summarized the main theories and evidence on woody plant adaptations to tolerate multiple abiotic stresses. Finally, we introduced the ecological and ecophysiological perspectives on this matter and placed the contributions to this VSI within the current state of the art. Altogether, this VSI allowed us to identify the lack of large-scale integration of patterns and processes describing woody plant adaptations to multiple abiotic stresses as a major gap in this field.}, journal={FLORA}, publisher={Elsevier BV}, author={Puglielli, Giacomo and Laanisto, Lauri and Gori, Antonella and Cardoso, Amanda A.}, year={2023}, month={Feb} }
 @article{freitas_oliveira_mcadam_lawson_damatta_cardoso_2023, title={Woody species grown under sun and shade present similar stomatal speed}, volume={7}, ISSN={["2197-0025"]}, url={http://dx.doi.org/10.1007/s40626-023-00283-3}, DOI={10.1007/s40626-023-00283-3}, journal={THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY}, publisher={Springer Science and Business Media LLC}, author={Freitas, Rafael S. and Oliveira, Leonardo A. and McAdam, Scott A. M. and Lawson, Tracy and DaMatta, Fabio M. and Cardoso, Amanda A.}, year={2023}, month={Jul} }
 @article{mcadam_kane_reyes_cardoso_brodribb_2022, title={An abrupt increase in foliage ABA levels on incipient leaf death occurs across vascular plants}, volume={3}, url={https://publons.com/wos-op/publon/49611968/}, DOI={10.1111/PLB.13404}, abstractNote={Forest mortality during drought has been attributed to hydraulic failure, which can be challenging to measure. A limited number of alternative proxies for incipient leaf death exist. Here we investigate whether a terminal increase in abscisic acid (ABA) levels in leaves occurs across vascular land plants and is an indicator of imminent leaf death. For different species across vascular plants, we monitored ABA levels during lethal drought as well as leaf embolism resistance, across the canopy as leaves die following senescence, or when leaves are exposed to a heavy, lethal frost late in the growing season. We observed a considerable increase in foliage ABA levels once leaves showed signs of incipient leaf death. This increase in ABA levels upon incipient leaf death, could be induced by embolism during drought, by freezing or as leaves age naturally, and was observed in species spanning the phylogeny of vascular land plants as well as in an ABA biosynthetic mutant plant. A considerable increase in foliage ABA levels may act as an indicator of impending leaf death.}, journal={Plant Biology}, publisher={Wiley}, author={McAdam, S. A. M. and Kane, C. N. and Reyes, J. A. Mercado and Cardoso, A. A. and Brodribb, T. J.}, editor={Rühr, N.K.Editor}, year={2022} }
 @article{cardoso_2022, title={Consequences of saline-dry conditions to the soil-plant-air continuum}, volume={7}, ISSN={["1532-2548"]}, url={http://dx.doi.org/10.1093/plphys/kiac343}, DOI={10.1093/plphys/kiac343}, journal={PLANT PHYSIOLOGY}, publisher={Oxford University Press (OUP)}, author={Cardoso, Amanda A.}, year={2022}, month={Jul} }
 @article{oliveira_cardoso_andrade_pereira_araujo_santos_damatta_martins_2022, title={Exploring leaf hydraulic traits to predict drought tolerance of Eucalyptus clones}, volume={4}, ISSN={["1758-4469"]}, url={https://publons.com/wos-op/publon/52051363/}, DOI={10.1093/TREEPHYS/TPAC040}, abstractNote={Ongoing changes in climate, and the consequent mortality of natural and cultivated forests across the globe, highlight the urgent need to understand the plant traits associated with greater tolerance to drought. Here, we aimed at assessing key foliar traits, with a focus on the hydraulic component, that could confer a differential ability to tolerate drought in three commercial hybrids of the most important Eucalyptus species utilized in tropical silviculture: E. urophyla, E. grandis and E. camaldulensis. All genotypes exhibited similar water potential when the 90% stomatal closure (Ψgs90) occurs with Ψgs90 always preceding the start of embolism events. The drought-tolerant hybrid showed a higher leaf resistance to embolism, but the leaf hydraulic efficiency was similar among all genotypes. Other traits presented by the drought-tolerant hybrid were a higher cell wall reinforcement, lower value ​​of osmotic potential at full turgor and greater bulk modulus of elasticity. We also identified that the leaf capacitance after the turgor loss, the ratio between cell wall thickness (t) and lumen breadth (b) ratio (t/b)3, and the minimal conductance might be good proxies for screening drought-tolerant Eucalyptus genotypes. Our findings suggest that xylem resistance to embolism can be an important component of drought tolerance in Eucalyptus in addition to other traits aimed at delaying the development of high tensions in the xylem.}, journal={TREE PHYSIOLOGY}, publisher={Oxford University Press (OUP)}, author={Oliveira, Leonardo A. and Cardoso, Amanda A. and Andrade, Moab T. and Pereira, Talitha S. and Araujo, Wagner L. and Santos, Gleison A. and Damatta, Fabio M. and Martins, Samuel C.}, year={2022}, month={May} }
 @article{andrade_oliveira_pereira_cardoso_batista-silva_damatta_zsogon_martins_2022, title={Impaired auxin signaling increases vein and stomatal density but reduces hydraulic efficiency and ultimately net photosynthesis}, ISSN={["1460-2431"]}, url={https://publons.com/wos-op/publon/50142161/}, DOI={10.1093/JXB/ERAC119}, abstractNote={Auxins are known to regulate xylem development in plants, however, their effects on water transport efficiency are poorly known. Here we used tomato plants of the diageotropica mutant (dgt), which has impaired function of a Cyclophilin 1 cis/trans isomerase involved in auxin signaling, and its corresponding wild type (WT) to explore its effects on plant hydraulics and leaf gas exchange. The xylem conduits of dgt showed a reduced hydraulically-weighted vessel diameter (Dh) (24-43%) and conduit number (25-58%) in petioles and stems, resulting in lower theoretical hydraulic conductivities (Kt); on the other hand, no changes in root Dh and Kt were observed. The measured stem and leaf hydraulic conductances of dgt agreed with the Kt values and were lower (up to 81%) as well; however, despite dgt and WT showed similar root Dh and Kt, the measured root hydraulic conductance of dgt was 75% lower. The dgt mutation increased the vein and stomata density, which could potentially increase photosynthesis. Nevertheless, even presenting the same photosynthetic capacity of WT plants, the dgt showed a photosynthetic rate c. 25% lower, coupled with a stomatal conductance reduction of 52%. These results clearly demonstrate that increases in Dv and Ds only result in higher leaf gas exchange when accompanied by higher hydraulic efficiency.}, journal={JOURNAL OF EXPERIMENTAL BOTANY}, publisher={Oxford University Press (OUP)}, author={Andrade, Moab T. and Oliveira, Leonardo A. and Pereira, Talitha S. and Cardoso, Amanda A. and Batista-Silva, Willian and DaMatta, Fabio M. and Zsogon, Aguston and Martins, Samuel C.}, year={2022}, month={Mar} }
 @article{cardoso_2022, title={Linking leaf embolism resistance with pit membrane characteristics}, volume={6}, ISSN={["1532-2548"]}, url={https://publons.com/wos-op/publon/52935726/}, DOI={10.1093/plphys/kiac293}, abstractNote={Long-distance transport of water is essential for plant function and this process is largely facilitated by the xylem. Water is transported under negative pressure within xylem conduits (i.e. unicellular tracheids and multicellular vessels) and between adjacent conduits through the pit membranes of interconduit pits (Figure 1). In some species, pit apertures are lined with branched or irregularly shaped protuberances called vestures and such pits are referred to as vestured pits. In angiosperms, pit membranes are homogeneous, modified primary cell walls consisting of microfibril layers that form a three-dimensional porous matrix (Kaack et al., 2019). This matrix is composed of solid material (i.e. cellulose) forming numerous geometrically irregular pore spaces that are interconnected through pore constrictions of varying sizes. Such constrictions strongly influence the hydraulic resistance of fluid transport across the pit membrane (Figure 1) (for a three-dimensional representation of pit membranes, pore spaces, and pore constrictions, see Kaack et al. [2019] and Zhang et al. [2020]). During soil drought, increasing negative pressure of the xylem above a threshold results in the movement of gas from neighboring gas-filled conduits into the lumen of sap-filled conduits through pit membrane pores. Gas entry into a sapfilled conduit increases the likelihood that a large bubble is formed, which can block water transport within that conduit (embolism), thereby reducing hydraulic conductances (Kaack et al., 2021). The xylem vulnerability to embolism of several plant organs can be quantified by vulnerability curves that describe the accumulation of embolisms as a function of water potential. The water potential at which 50% of the xylem is embolized (P50) represents the xylem resistance to embolism of that particular organ and species (Choat et al., 2012). Leaf embolism results in declines in leaf function and, ultimately, can cause leaf mortality (Cardoso et al., 2020; Brodribb et al., 2021). Such detrimental consequences demonstrate the importance of leaf embolism resistance to whole-plant drought tolerance. Yet, unlike the case with stems, the structural determinants of embolism resistance in leaves remain largely unknown. In this issue of Plant Physiology, Levionnois et al. (2022) assessed the linkage between leaf embolism resistance and pit membrane characteristics across Neotropical tree species, providing valuable insights into the xylem embolism resistance of leaves. One of the most interesting findings of this study is that the maximum pit membrane thickness (TPM) was highly predictive of leaf embolism resistance (represented by the P50), with thick pit membranes being associated with high leaf embolism resistance. This was true across non-vestured species at the individual level (when these two parameters were collected for adjacent leaves of the same shoot). Similar associations have been previously demonstrated at the stem level across different biomes, plant forms, and species. The mechanistic explanation for this association relies on the hypothesis that thick pit membranes have higher chances of presenting narrow pore constrictions because thick pit membranes can be considered as consisting of more cellulose layers and thus more pore constrictions than thin pit membranes (Kaack et al., 2021). The association between these two traits, however, did not hold true at the species level (i.e. when mean P50 was obtained from different individuals and TPM was obtained for a single individual per species). A possible explanation might be linked to the large intra-specific variation in leaf P50 observed for several species in this study (for the vulnerability curves of all species, see Supplementary Material of Levionnois et al. [2020]). It is possible (or even likely) that, like leaf P50, TPM might also exhibit a large variability across different leaves, especially from different individuals, yielding N ew s an d V ie w s}, journal={PLANT PHYSIOLOGY}, author={Cardoso, Amanda A.}, year={2022}, month={Jun} }
 @article{cardoso_kane_rimer_mcadam_2022, title={Seeing is believing: what visualising bubbles in the xylem has revealed about plant hydraulic function}, url={http://dx.doi.org/10.1071/fp21326}, DOI={10.1071/fp21326}, abstractNote={Maintaining water transport in the xylem is critical for vascular plants to grow and survive. The drought-induced accumulation of embolism, when gas enters xylem conduits, causes declines in hydraulic conductance (K) and is ultimately lethal. Several methods can be used to estimate the degree of embolism in xylem, from measuring K in tissues to directly visualising embolism in conduits. One method allowing a direct quantification of embolised xylem area is the optical vulnerability (OV) technique. This method has been used across different organs and has a high spatial and temporal resolution. Here, we review studies using the OV technique, discuss the main advantages and disadvantages of this method, and summarise key advances arising from its use. Vulnerability curves generated by the OV method are regularly comparable to other methods, including the centrifuge and X-ray microtomography. A major advantage of the OV technique over other methods is that it can be simultaneously used to determine in situ embolism formation in leaves, stems and roots, in species spanning the phylogeny of land plants. The OV method has been used to experimentally investigate the spreading of embolism through xylem networks, associate embolism with downstream tissue death, and observe embolism formation in the field.}, journal={Functional Plant Biology}, publisher={CSIRO Publishing}, author={Cardoso, Amanda A. and Kane, Cade N. and Rimer, Ian M. and McAdam, Scott A. M.}, year={2022} }
 @article{storage temperatures for bowdichia virgilioides seeds: an endangered species_2022, url={https://publons.com/wos-op/publon/51630199/}, DOI={10.31285/AGRO.26.678}, abstractNote={Bowdichia virgilioides Kunth is a tree species native to the Brazilian Cerrado that has been listed as an endangered species due to its overexploitation in the last decades. Given its slow and difficult propagation in natural environments, propagation in plant nurseries appears as an interesting strategy to preserve this endangered species. Therefore, in this study we employed a wide range of temperatures (i.e. from -25°C to -196°C) to store seeds of B. virgilioides in the short-term, and evaluated its efficiency by assessing seed germination rate, speed index, time and synchrony, as well as seedling growth and quality. Germination percentage was only slightly negatively affected by storage time (up to 32 weeks) at 25°C. Despite that, after 32 weeks of storage, all storage temperatures resulted in similar seed germination parameters and seedling growth and quality. Overall, our results highlight that the seeds of this species can be stored in the short-term at a wide range of temperatures, facilitating large-scale propagation in plant nurseries.}, journal={Agrociencia (Montevideo)}, year={2022} }
 @article{burgess_cardoso_2022, title={Throwing shade: Limitations to photosynthesis at high planting densities and how to overcome them}, volume={191}, ISSN={["1532-2548"]}, url={http://dx.doi.org/10.1093/plphys/kiac567}, DOI={10.1093/plphys/kiac567}, number={2}, journal={PLANT PHYSIOLOGY}, publisher={Oxford University Press (OUP)}, author={Burgess, Alexandra J. and Cardoso, Amanda A.}, year={2022}, month={Dec} }
 @article{avila_guan_kane_cardoso_batz_damatta_jansen_mcadam_2022, title={Xylem embolism spread is largely prevented by interconduit pit membranes until the majority of conduits are gas‐filled}, volume={1}, url={https://publons.com/publon/50883240/}, DOI={10.1111/PCE.14253}, abstractNote={Xylem embolism resistance varies across species influencing drought tolerance, yet little is known about the determinants of the embolism resistance of an individual conduit. Here we conducted an experiment using the optical vulnerability method to test whether individual conduits have a specific water potential threshold for embolism formation and whether pre-existing embolism in neighbouring conduits alters this threshold. Observations were made on a diverse sample of angiosperm and conifer species through a cycle of dehydration, rehydration and subsequent dehydration to death. Upon rehydration after the formation of embolism, no refilling was observed. When little pre-existing embolism was present, xylem conduits had a conserved, individual embolism-resistance threshold that varied across the population of conduits. The consequence of a variable conduit-specific embolism threshold is that a small degree of pre-existing embolism in the xylem results in apparently more resistant xylem in subsequent dehydrations, particularly in angiosperms with vessels. While our results suggest that pit membranes separating xylem conduits are critical for maintaining a conserved individual conduit threshold for embolism when little pre-existing embolism is present, as the percentage of embolized conduits increases, gas movement, local pressure differences and connectivity between conduits increasingly contribute to embolism spread.}, journal={Plant Cell & Environment}, publisher={Wiley}, author={Avila, Rodrigo T. and Guan, Xinyi and Kane, Cade N. and Cardoso, Amanda A. and Batz, Timothy A. and DaMatta, Fábio M. and Jansen, Steven and McAdam, Scott A. M.}, year={2022} }
 @article{toral-juárez_avila_cardoso_brito_machado_almeida_souza_martins_damatta_2021, title={Drought-tolerant coffee plants display increased tolerance to waterlogging and post-waterlogging reoxygenation}, volume={11}, url={https://publons.com/wos-op/publon/35026503/}, DOI={10.1016/J.ENVEXPBOT.2020.104311}, abstractNote={Soil waterlogging negatively impacts plants due to reduced oxygen availability in the rhizosphere. Here we selected two Coffea canephora clones with contrasting tolerance to drought and oxidative stress to test whether they would also exhibit contrasting tolerance to waterlogging and post-waterlogging reoxygenation. Plants were exposed to six days of waterlogging followed by 40 days of reoxygenation. During waterlogging, both clones exhibited lower leaf gas exchange rate, electron transport rate and photochemical quenching as well as increased non-photochemical quenching; overall, these changes were stronger in the drought tolerant (DT) clones over the drought sentitive (DS) ones. Photosynthetic limitations were essentially linked to diffusional constraints regardless of clone. In both clones, declines in stomatal conductance were not associated with foliar ABA or ethylene levels, however stomatal conductance correlated with the plant hydraulic conductance. After reoxygenation, DT plants mostly recovered leaf function and plant mortality was less than 10%. Conversely, DS plants displayed very low water potentials after reoxygenation, which was accompanied by photosynthetic and membrane damage and 40% plant mortality. Our findings confirmed that the DT clone is less impaired by soil waterlogging and reoxygenation than the DS one. The differences between clones were more evident during the reoxygenation than during waterlogging, likely due to the drought experienced by the DS but not DT plants. Collectively, this information leads us to a common path to find new coffee genotypes with increased tolerance to both drought and waterlogging aiming at increased coffee sustainability under an ongoing climate changing scenario.}, journal={Environmental and Experimental Botany}, publisher={Elsevier BV}, author={Toral-Juárez, Marco A. and Avila, Rodrigo T. and Cardoso, Amanda A. and Brito, Fred A.L. and Machado, Kleiton L.G. and Almeida, Wellington L. and Souza, Raylla P.B. and Martins, Samuel C.V. and DaMatta, Fábio M.}, year={2021}, pages={104311} }
 @article{limited plasticity in embolism resistance in response to light in leaves and stems in species with considerable vulnerability segmentation_2021, url={https://publons.com/wos-op/publon/45018564/}, DOI={10.1111/PPL.13450}, abstractNote={Xylem resistance to embolism is a key metric determining plant survival during drought. Yet, we have a limited understanding of the degree of plasticity in vulnerability to embolism. Here, we tested whether light availability influences embolism resistance in leaves and stems. The optical vulnerability method was used to assess stem and leaf resistance to embolism in Phellodendron amurense and Ilex verticillata acclimated to sun and shade microenvironments within the same canopy. In both species we found considerable segmentation in xylem resistance to embolism between leaves and stems, but only minor acclimation in response to light availability. With the addition of a third species, Betula pubescens, which shows no vulnerability segmentation, we sought to investigate any xylem anatomical traits that might correlate with strong vulnerability segmentation. We found a correlation between the area fraction of vessels in the xylem and embolism resistance across all species and tissue types. Our results suggest that minimal acclimation of embolism resistance occurs in response to light environment in the same individual and that the degree of vulnerability segmentation between leaves and stems might be determined by the vessel lumen fraction of the xylem.}, journal={Physiologia Plantarum}, year={2021} }
 @article{da-silva_shimoia_posso_cardoso_batz_oliveira_amarante_2021, title={Nitrate nutrition increases foliar levels of nitric oxide and waterlogging tolerance in soybean}, volume={43}, url={http://dx.doi.org/10.1007/s11738-021-03291-5}, DOI={10.1007/s11738-021-03291-5}, number={8}, journal={Acta Physiologiae Plantarum}, publisher={Springer Science and Business Media LLC}, author={Da-Silva, Cristiane Jovelina and Shimoia, Eduardo P. and Posso, Douglas A. and Cardoso, Amanda A. and Batz, Timothy A. and Oliveira, Ana Claudia B. and Amarante, Luciano}, year={2021}, month={Aug} }
 @article{cardoso_barbosa_santos_2021, title={Optimum conditions for seed propagation of Garcinia brasiliensis: mimicking natural habitats allows better results}, volume={43}, url={http://dx.doi.org/10.1590/2317-1545v43248633}, DOI={10.1590/2317-1545v43248633}, abstractNote={Abstract: Garcinia brasiliensis is a tropical tree species of economic importance to local populations in the Amazon and Atlantic forests. The plant propagation of this species occurs mostly through seeds and despite that very little information about seedling emergence and growth is currently available. Here we assessed basic information on the seed and seedling ecophysiology of G. brasiliensis aiming at providing key details for seed propagation. Seeds are large and contain high levels of water when recently collected (c. 50%). Seed storage, especially under low temperature, resulted in very low seedling emergence. Fruit storage at c. 25 °C, on the other hand, was demonstrated to be the best option for maintaining seed viability in the short term. Removal of the seed coat considerably hastened and increased the percentage emergence of seedlings, despite the light condition. Similarly, a higher percentage and index of seedling emergence was observed under 35 °C over 25 °C, despite the substrate. Amongst all the substrates tested, sand resulted in lower seedling emergence. Cultivating seedlings in vermiculate and at 25 °C provided the best condition for root and shoot growth. Ideal conditions of light and temperature for seedling emergence and development mirror the environmental conditions under which plants of G. brasiliensis establish in nature.}, journal={Journal of Seed Science}, publisher={FapUNIFESP (SciELO)}, author={Cardoso, Amanda A. and Barbosa, Sandro and Santos, Breno R.}, year={2021} }
 @article{the interplay between irrigation and fruiting on branch growth and mortality, gas exchange and water relations of coffee trees_2021, url={https://publons.com/wos-op/publon/33938638/}, DOI={10.1093/TREEPHYS/TPAA116}, abstractNote={The overall coordination between gas exchanges and plant hydraulics may be affected by soil water availability and source-to-sink relationships. Here we evaluated how branch growth and mortality, leaf gas exchange, and metabolism are affected in coffee (Coffea arabica L.) trees by drought and fruiting. Field-grown plants were irrigated or not, and maintained with full or none fruit load. Under mild water deficit, irrigation per se did not significantly impact growth but markedly reduced branch mortality in fruiting trees, despite similar leaf assimilate pools and water status. Fruiting increased net photosynthetic rate in parallel to an enhanced stomatal conductance, particularly in irrigated plants. Mesophyll conductance and maximum RuBisCO carboxylation rate remained unchanged across treatments. The increased stomatal conductance in fruiting trees over non-fruiting ones was unrelated to internal CO2 concentration, foliar ABA levels, or differential ABA sensitivity. However, stomatal conductance was associated with higher stomatal density, lower stomatal sensitivity to vapor pressure deficit, and higher leaf hydraulic conductance and capacitance. Increased leaf transpiration rate in fruiting trees was supported by coordinated alterations in plant hydraulics, which explained the maintenance of plant water status. Finally, by preventing branch mortality, irrigation can mitigate biennial production fluctuations and improve the sustainability of coffee plantations.}, journal={Tree Physiology}, year={2021} }
 @article{cardoso_gori_da-silva_brunetti_2020, title={Abscisic Acid Biosynthesis and Signaling in Plants: Key Targets to Improve Water Use Efficiency and Drought Tolerance}, volume={10}, url={https://www.mdpi.com/2076-3417/10/18/6322}, DOI={10.3390/app10186322}, abstractNote={The observation of a much-improved fitness of wild-type plants over abscisic acid (ABA)-deficient mutants during drought has led researchers from all over to world to perform experiments aiming at a better understanding of how this hormone modulates the physiology of plants under water-limited conditions. More recently, several promising approaches manipulating ABA biosynthesis and signaling have been explored to improve water use efficiency and confer drought tolerance to major crop species. Here, we review recent progress made in the last decade on (i) ABA biosynthesis, (ii) the roles of ABA on plant-water relations and on primary and secondary metabolisms during drought, and (iii) the regulation of ABA levels and perception to improve water use efficiency and drought tolerance in crop species.}, number={18}, journal={Applied Sciences}, publisher={MDPI AG}, author={Cardoso, Amanda A. and Gori, Antonella and Da-Silva, Cristiane Jovelina and Brunetti, Cecilia}, year={2020}, month={Sep}, pages={6322} }
 @article{coffee plants respond to drought and elevated [co2] through changes in stomatal function, plant hydraulic conductance, and aquaporin expression_2020, url={http://dx.doi.org/10.1016/j.envexpbot.2020.104148}, DOI={10.1016/j.envexpbot.2020.104148}, abstractNote={Rising air CO2 concentration ([CO2]) is believed to mitigate the negative impacts of global climate changes such as increased air temperatures and drought events on plant growth and survival. Nonetheless, how elevated [CO2] affects the way coffee (Coffea arabica L.) plants sense and respond to drought remains a critical unknown. In this study, potted coffee plants were cultivated under two air [CO2] (ca. 400 ppm or 700 ppm) in open top chambers under greenhouse conditions. After a 5-month exposure to [CO2] treatments, plants were submitted to a progressive, controlled soil water deficit down to 20 % soil field capacity. Under well-watered (100 % field capacity) conditions, 700-plants displayed lower whole-plant transpiration rates (T) than their 400-counterparts. Changes in T were unrelated to stomatal conductances at the leaf scale (as well as stomatal morphology) or foliar ABA levels, but they were rather associated with faster stomata closure rates upon rapid increases in vapor pressure deficit in the 700-plants. During drought, 700-plants were able to maintain higher water potentials and plant hydraulic conductances for longer in parallel to higher T than their 400-counterparts. Under elevated [CO2], the faster stomatal closure rates (irrigated conditions) or the maintenance of plant hydraulic conductances (drought conditions) were associated with higher (3 to 40-fold) transcript abundance of most aquaporin genes. Altogether, our results suggest that elevated [CO2] has marked implications on how coffee plants respond to soil water deficit, ultimately permitting 700-plants to have improved fitness under drought when compared to 400-plants.}, journal={Environmental and Experimental Botany}, year={2020}, month={Sep} }
 @article{drought‐induced lacuna formation in the stem causes hydraulic conductance to decline before xylem embolism in
            selaginella_2020, url={http://dx.doi.org/10.1111/nph.16649}, DOI={10.1111/nph.16649}, abstractNote={Lycophytes are the earliest diverging extant lineage of vascular plants, sister to all other vascular plants. Given that most species are adapted to ever-wet environments, it has been hypothesized that lycophytes, and by extension the common ancestor of all vascular plants, have few adaptations to drought. We investigated the responses to drought of key fitness-related traits such as stomatal regulation, shoot hydraulic conductance (Kshoot ) and stem xylem embolism resistance in Selaginella haematodes and S. pulcherrima, both native to tropical understory. During drought stomata in both species were found to close prior to declines in Kshoot , with a 50% loss of Kshoot occurring at -1.7 and -2.5 MPa in S. haematodes and S. pulcherrima, respectively. Direct observational methods revealed that the xylem of both species was resistant to embolism formation, with 50% of embolised xylem area occurring at -3.0 and -4.6 MPa in S. haematodes and S. pulcherrima, respectively. X-ray micro-computed tomography images of stems revealed that the decline in Kshoot occurred with the formation of an air-filled lacuna, disconnecting the central vascular cylinder from the cortex. We propose that embolism resistant xylem and large capacitance, provided by collapsing inner cortical cells, is essential for Selaginella survival during water deficit.}, journal={New Phytologist}, year={2020}, month={May} }
 @article{leaf hydraulic properties are decoupled from leaf area across coffee species_2020, url={http://dx.doi.org/10.1007/s00468-020-01983-y}, DOI={10.1007/s00468-020-01983-y}, journal={Trees}, year={2020}, month={May} }
 @article{new developments in understanding plant water transport under drought stress_2020, url={https://publons.com/wos-op/publon/33073430/}, DOI={10.1111/NPH.16663}, abstractNote={The Fourth Xylem International Meeting (XIM4) brought together over 100 plant scientists to discuss a wide range of topics related to embolism resistance and efficiency of long-distance water transport through plants, and their implications for cultivated and natural systems. The diversity of methods, plant species, and physiological processes discussed in the context of water stress at the meeting highlighted a need to integrate spatial and temporal data about plant functioning, specifically in regard to climate-change-related challenges.}, journal={New Phytologist}, year={2020} }
 @article{osmotic adjustment and hormonal regulation of stomatal responses to vapor pressure deficit in sunflower_2020, DOI={https://doi.org/10.1093/aobpla/plaa025}, abstractNote={Dynamic variation of the stomatal pore in response to changes in leaf-air vapour pressure difference (VPD) constitutes a critical regulation of daytime gas exchange. The stomatal response to VPD has been associated with both foliage abscisic acid (ABA) and leaf water potential (Ψ l ); however, causation remains a matter of debate. Here, we seek to separate hydraulic and hormonal control of stomatal aperture by manipulating the osmotic potential of sunflower leaves. In addition, we test whether stomatal responses to VPD in an ABA-deficient mutant (w-1) of sunflower are similar to the wild type. Stomatal apertures during VPD transitions were closely linked with foliage ABA levels in sunflower plants with contrasting osmotic potentials. In addition, we observed that the inability to synthesize ABA at high VPD in w-1 plants was associated with no dynamic or steady-state stomatal response to VPD. These results for sunflower are consistent with a hormonal, ABA-mediated stomatal responses to VPD rather than a hydraulic-driven stomatal response to VPD.}, journal={AoB Plants}, year={2020}, month={Jun} }
 @article{osmotic adjustment and hormonal regulation of stomatal responses to vapour pressure deficit in sunflower_2020, url={https://publons.com/wos-op/publon/31689867/}, DOI={10.1093/AOBPLA/PLAA025}, abstractNote={Abstract Dynamic variation of the stomatal pore in response to changes in leaf–air vapour pressure difference (VPD) constitutes a critical regulation of daytime gas exchange. The stomatal response to VPD has been associated with both foliage abscisic acid (ABA) and leaf water potential (Ψ l); however, causation remains a matter of debate. Here, we seek to separate hydraulic and hormonal control of stomatal aperture by manipulating the osmotic potential of sunflower leaves. In addition, we test whether stomatal responses to VPD in an ABA-deficient mutant (w-1) of sunflower are similar to the wild type. Stomatal apertures during VPD transitions were closely linked with foliage ABA levels in sunflower plants with contrasting osmotic potentials. In addition, we observed that the inability to synthesize ABA at high VPD in w-1 plants was associated with no dynamic or steady-state stomatal response to VPD. These results for sunflower are consistent with a hormonal, ABA-mediated stomatal responses to VPD rather than a hydraulic-driven stomatal response to VPD.}, journal={AoB Plants}, year={2020} }
 @article{starch accumulation does not lead to feedback photosynthetic downregulation in girdled coffee branches under varying source-to-sink ratios_2020, url={https://publons.com/publon/21671015/}, DOI={10.1007/S00468-019-01893-8}, journal={Trees}, year={2020} }
 @article{xylem embolism resistance determines leaf mortality during drought in persea americana_2020, url={https://publons.com/wos-op/publon/27738638/}, DOI={10.1104/PP.19.00585}, abstractNote={Leaf death in avocado during drought is strongly associated with embolism in the xylem. The driver of leaf mortality during drought stress is a critical unknown. We used the commercially important tree Persea americana, in which there is a large variation in the degree of drought-induced leaf death across the canopy, to test whether embolism formation in the xylem during drought drives this leaf mortality. A large range in the number of embolized vessels in the petioles of leaves was observed across the canopy of plants that had experienced drought. Despite considerable variation between leaves, the amount of embolized vessels in the xylem of the petiole strongly correlated with area of drought-induced tissue death in individual leaves. Consistent with this finding was a large interleaf variability in xylem resistance to embolism, with a 1.45 MPa variation in the water potential at which 50% of the xylem in the leaf midrib embolized across leaves. Our results implicate xylem embolism as a driver of leaf mortality during drought. Moreover, we propose that heterogeneity in drought-induced leaf mortality across a canopy is caused by high interleaf variability in xylem resistance to embolism, which may act as a buffer against complete canopy death during prolonged drought in P. americana.}, journal={Plant Physiology}, year={2020} }
 @inbook{souri_cardoso_da‐silva_oliveira_dari_sihi_karimi_2019, title={Heavy Metals and Photosynthesis: Recent Developments}, url={http://dx.doi.org/10.1002/9781119501800.ch7}, DOI={10.1002/9781119501800.CH7}, abstractNote={Heavy metals are among the main pollutants affecting plant photosynthesis. A broad literature screening reveals that heavy metals impair, in a type- and dose-dependent manner, many aspects related to the photosynthetic apparatus. This chapter explores how stomatal and mesophyll conductances, chloroplasts, photosynthetic pigments, photosystems I and II, photosynthetic enzymes, and the antioxidant defense mechanism are negatively affected by heavy metals. It also describes how hyperaccumulator plants cope with potential disturbances in photosynthesis upon heavy metal stress.}, booktitle={Photosynthesis, Productivity and Environmental Stress}, publisher={Wiley}, author={Souri, Zahra and Cardoso, Amanda A. and da‐Silva, Cristiane J. and Oliveira, Letúzia M. and Dari, Biswanath and Sihi, Debjani and Karimi, Naser}, year={2019}, month={Sep}, pages={107–134} }
 @article{how do coffee trees deal with severe natural droughts? an analysis of hydraulic, diffusive and biochemical components at the leaf level_2019, url={https://publons.com/publon/21671017/}, DOI={10.1007/S00468-019-01889-4}, journal={Trees}, year={2019} }
 @article{cardoso_randall_mcadam_2019, title={Hydraulics Regulate Stomatal Responses to Changes in Leaf Water Status in the Fern Athyrium filix-femina}, volume={179}, url={https://publons.com/wos-op/publon/15259785/}, DOI={10.1104/PP.18.01412}, abstractNote={Stomatal responses to water status in ferns are highly predictable and not functionally regulated by the hormone abscisic acid. Stomatal responses to changes in leaf water status are important for the diurnal regulation of gas exchange and the survival of plants during drought. These stomatal responses in angiosperm species are well characterized, yet in species of nonseed plants, an ongoing debate surrounds the role of metabolism, particularly the role of the hormone abscisic acid (ABA), in functionally regulating stomatal responses to changes in leaf water status. Here, we measured the stomatal response to changes in vapor pressure difference (VPD) in two natural forms of the fern species Athyrium filix-femina, recently suggested to have stomata that are regulated by ABA. The two forms measured had considerable differences in key hydraulic traits, including leaf hydraulic conductance and capacitance, as well as the kinetics of stomatal response to changes in VPD. In both forms, the stomatal responses to VPD could be accurately predicted by a dynamic, mechanistic model that assumes guard cell turgor changes in concert with leaf turgor in the light, and not via metabolic processes including the level of ABA. During drought, endogenous ABA did not play a role in stomatal closure, and exogenous ABA applied to live, intact leaves did not induce stomatal closure. Our results indicate that functional stomatal responses to changes in leaf water status in ferns are regulated by leaf hydraulics and not metabolism. With ferns being sister to seed plants, this result has implications for the evolutionary reconstruction of functional stomatal responses across vascular land plant lineages.}, number={2}, journal={Plant Physiology}, author={Cardoso, A.A. and Randall, J.M. and McAdam, S.A.M.}, year={2019}, pages={533–543} }
 @article{misleading conclusions from exogenous aba application: a cautionary tale about the evolution of stomatal responses to changes in leaf water status_2019, url={https://publons.com/wos-op/publon/20513320/}, DOI={10.1080/15592324.2019.1610307}, abstractNote={ABSTRACT Stomatal responses to changes in leaf water status are critical for minimizing excessive water loss during soil drought. A major debate has surrounded the evolution of stomatal responses to water status and this debate has particularly focused on the evolution of the regulatory role of the drought hormone abscisic acid (ABA). Studies relying on the application of high levels of exogenous ABA have occasionally concluded that all stomata respond to ABA and that stomatal regulation in response to this hormone has not evolved over the past 450 million years. In contrast, studies which have investigated stomatal function in intact plants, as well as the role of endogenous ABA in regulating stomatal aperture, have found major evolutionary transitions in the functional regulation of stomata across land plant lineages. We show that endogenous ABA plays no role in closing the stomata of the fern Nephrolepis exaltata during natural soil drought, in contrast to a recent finding using isolated epidermis and exceptionally high levels of exogenous ABA. We conclude that stomatal behavior in intact plants has evolved over time, and may have shaped the evolutionary and ecological success of successive land plant lineages.}, journal={Plant Signaling and Behavior}, year={2019} }
 @article{mcadam_cardoso_2019, title={The recurrent evolution of extremely resistant xylem}, volume={76}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85058939329&partnerID=MN8TOARS}, DOI={10.1007/s13595-018-0786-7}, abstractNote={Highly resistant xylem has evolved multiple times over the past 400 million years. Water is transported under tension in xylem and consequently is vulnerable to invasion by air and the formation of embolism. A debate has raged over whether embolism formation is non-reversible occurring at low water potentials or a regular diurnal occurrence that is non-lethal because of a capacity to refill embolised conduits. This commentary is on a recent article, which utilised new non-invasive imaging techniques for assessing the formation of embolism in xylem, finding that the xylem of Laurus nobilis was highly resistant to the formation of embolism. The recent results of this discovery are placed in the context knowledge from a diversity of species that has so far been identified with xylem similarly highly resistant to embolism formation. The discovery that L. nobilis has xylem highly resistant to embolism formation adds to a body of literature suggesting that the resistance of xylem to embolism formation is a key adaptation utilised by many species native to seasonally dry environments. Highly resistant xylem has evolved numerous times across the angiosperm clade. With more studies utilising similar observational and direct methods of assessing embolism resistance, further insight into the ecological and evolutionary relevance of this trait is imminent.}, number={1}, journal={Annals of Forest Science}, author={McAdam, S.A.M. and Cardoso, A.A.}, year={2019} }
 @article{cardoso_brodribb_lucani_damatta_mcadam_2018, title={Coordinated plasticity maintains hydraulic safety in sunflower leaves.}, volume={41}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:29748980&KeyUID=MEDLINE:29748980}, DOI={10.1111/pce.13335}, abstractNote={The xylem cavitation threshold water potential establishes a hydraulic limit on the ability of woody species to survive in water-limiting environments, but herbs may be more plastic in terms of their ability to adapt to drying conditions. Here, we examined the capacity of sunflower (Helianthus annuus L.) leaves to adapt to reduced water availability by modifying the sensitivity of xylem and stomata to soil water deficit. We found that sunflower plants grown under water-limited conditions significantly adjusted leaf osmotic potential, which was linked to a prolongation of stomatal opening as soil dried and a reduced sensitivity of photosynthesis to water-stress-induced damage. At the same time, the vulnerability of midrib xylem to water-stress-induced cavitation was observed to be highly responsive to growth conditions, with water-limited plants producing conduits with thicker cell walls which were more resistant to xylem cavitation. Coordinated plasticity in osmotic potential and xylem vulnerability enabled water-limited sunflowers to safely extract water from the soil, while protecting leaf xylem against embolism. High plasticity in sunflower xylem contrasts with data from woody plants and may suggest an alternative strategy in herbs.}, number={11}, journal={Plant, cell & environment}, author={Cardoso, Amanda A and Brodribb, Timothy J and Lucani, Christopher J and DaMatta, Fabio M and McAdam, Scott A M}, year={2018}, pages={2567–2576} }
 @article{cardoso_randall_jordan_mcadam_2018, title={Extended differentiation of veins and stomata is essential forthe expansion of large leaves in Rheum rhabarbarum.}, volume={105}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:30475383&KeyUID=MEDLINE:30475383}, DOI={10.1002/ajb2.1196}, abstractNote={PREMISE OF THE STUDY
The densities of veins and stomata govern leaf water supply and gas exchange. They are coordinated to avoid overproduction of either veins or stomata. In many species, where leaf area is greater at low light, this coordination is primarily achieved through differential cell expansion, resulting in lower stomatal and vein density in larger leaves. This mechanism would, however, create highly inefficient leaves in species in which leaf area is greater at high light. Here we investigate the role of cell expansion and differentiation as regulators of vein and stomatal density in Rheum rhabarbarum, which produces large leaves under high light.


METHODS
Rheum rhabarbarum plants were grown under full sunlight and 7% of full sunlight. Leaf area, stomatal density, and vein density were measured from leaves harvested at different intervals.


KEY RESULTS
Leaves of R. rhabarbarum expanded at high light were six times larger than leaves expanded at low light, yet vein and stomatal densities were similar. In high light-expanded leaves, minor veins were continuously initiated as the leaves expanded, while an extended period of stomatal initiation, compared to leaves expanded at low light, occurred early in leaf development.


CONCLUSIONS
We demonstrate that R. rhabarbarum adjusts the initiation of stomata and minor veins at high light, allowing for the production of larger leaves uncoupled from lower vein and stomatal densities. We also present evidence for an independent control of vein and stomatal initiation, suggesting that this adjustment must involve some unknown developmental mechanism.}, number={12}, journal={American journal of botany}, author={Cardoso, Amanda A and Randall, Joshua M and Jordan, Gregory J and McAdam, Scott A M}, year={2018}, pages={1967–1974} }
 @article{zhang_sussmilch_nichols_cardoso_brodribb_mcadam_2018, title={Leaves, not roots or floral tissue, are the main site of rapid, external pressure-induced ABA biosynthesis in angiosperms}, volume={69}, url={https://publons.com/wos-op/publon/3397429/}, DOI={10.1093/JXB/ERX480}, abstractNote={Abstract Rapid biosynthesis of abscisic acid (ABA) in the leaf, triggered by a decrease in cell volume, is essential for a functional stomatal response. However, it is not known whether rapid biosynthesis of ABA is also triggered in other plant tissues. Through the application of external pressure to flower, root, and leaf tissues, we test whether a reduction in cell volume can trigger rapid increases in ABA levels across the plant body in two species, Solanum lycopersicum and Passiflora tarminiana. Our results show that, in contrast to rapid ABA synthesis in the leaf, flower and root tissue did not show a significant, increase in ABA level in response to a drop in cell volume over a short time frame, suggesting that rapid ABA biosynthesis occurs only in leaf, not in flower or root tissues. A gene encoding the key, rate-limiting carotenoid cleavage enzyme (9-cis-epoxycarotenoid dioxygenase, NCED) in the ABA biosynthetic pathway in S. lycopersicum, NCED1, was upregulated to a lesser degree in flowers and roots compared with leaves in response to applied pressure. In both species, floral tissues contained substantially lower levels of the NCED substrate 9’-cis-neoxanthin than leaves, and this ABA precursor could not be detected in roots. Slow and minimal ABA biosynthesis was detected after 2 h in petals, indicating that floral tissue is capable of synthesizing ABA in response to sustained water deficit. Our results indicate that rapid ABA biosynthesis predominantly occurs in the leaves, and not in other tissues.}, number={5}, journal={Journal of Experimental Botany}, author={Zhang, Feng-Ping and Sussmilch, Frances and Nichols, David S. and Cardoso, Amanda A. and Brodribb, Timothy J. and McAdam, Scott A. M.}, year={2018}, pages={1261–1267} }
 @article{de souza_dias_pimenta_cardoso_pires_alvarenga_pícoli_2018, title={Morpho-anatomical, physiological and biochemical changes in rubber tree seeds}, volume={90}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85048643566&partnerID=MN8TOARS}, DOI={10.1590/0001-3765201820170340}, abstractNote={The physical, physiological and biochemical changes during the development until the dispersal of rubber tree seeds were evaluated with the purpose of estimating the point at physiological maturity. A total of 30 plants were selected at different points in a commercial planting area and had their flowers marked during the anthesis and every 15 days after marking. Fruits and seeds were collected for analysis of moisture content, dry matter, diameter and length. Details of the anatomy ultra-structure of the seeds were evaluated. The seed emergence, emergency speed index, heat resistant proteins and oxidative stress enzymes were examined. It was observed that fruits reached maximum size at 120 days after anthesis and seeds at 150 days. The seeds acquired germination capacity after 150 days. At 175 days, they presented the highest percentage of dry matter and lowest moisture, in addition to a higher percentage of germination and vigor. Therefore, it was possible to conclude that the physiological maturity of the rubber tree seeds occurs at 175 days after anthesis, and coincides with its maximum physiological quality. At 175 and 180 days post-anthesis, there is a greater expression of heat resistant proteins as well as low molecular weight and greater oxidative stress enzyme activity.}, number={2}, journal={Anais da Academia Brasileira de Ciencias}, author={De Souza, G.A. and Dias, D.C.F.S. and Pimenta, T.M. and Cardoso, A.?. and Pires, R.M.O. and Alvarenga, A.P. and Pícoli, E.A.T.}, year={2018}, pages={1625–1641} }
 @article{da-silva_canatto_cardoso_ribeiro_de oliveira_2018, title={Oxidative stress triggered by arsenic in a tropical macrophyte is alleviated by endogenous and exogenous nitric oxide}, volume={41}, ISSN={0100-8404 1806-9959}, url={http://dx.doi.org/10.1007/s40415-017-0431-y}, DOI={10.1007/S40415-017-0431-Y}, abstractNote={Nitric oxide (NO) plays important role in alleviating abiotic stresses in plants, including those caused by arsenic (As). Here, we examined the effects of endogenous and exogenous NO in Spirodela intermedia W. Koch (Lemnaceae) under As exposure. For this purpose, we evaluated the As content, reactive oxygen species (ROS) levels, membrane damage and enzymatic antioxidant system. The levels of endogenous NO and the activity of nitrate reductase (NR) were also addressed. The As treatment triggered the production of high endogenous levels of NO and a pronounced activation of the antioxidant enzymes; however, it was not sufficient to completely avoid the increment in ROS content and membrane damage. In contrast, exogenous NO decreased the As levels in plants exposed to As and NO donor, mitigating the ROS production and membrane damage, while maintaining a lower activity of the antioxidant enzymes compared with As-treated plants. Exogenous NO further downregulated the NR activity by a negative feedback, while As boosted the NR activity, consistent with the high endogenous levels of NO observed upon As treatment. Our results suggest that both endogenous and exogenous NO play critical roles in alleviating the As-induced oxidative stress in S. intermedia by reducing As uptake, and possibly by acting as an antioxidant molecule.}, number={1}, journal={Brazilian Journal of Botany}, publisher={Springer Science and Business Media LLC}, author={da-Silva, Cristiane Jovelina and Canatto, Regiane Aparecida and Cardoso, Amanda Avila and Ribeiro, Cleberson and de Oliveira, Juraci Alves}, year={2018}, pages={21–28} }
 @article{damatta_avila_cardoso_martins_ramalho_2018, title={Physiological and Agronomic Performance of the Coffee Crop in the Context of Climate Change and Global Warming: A Review}, volume={66}, url={https://publons.com/publon/3397435/}, DOI={10.1021/ACS.JAFC.7B04537}, abstractNote={Coffee is one of the most important global crops and provides a livelihood to millions of people living in developing countries. Coffee species have been described as being highly sensitive to climate change, as largely deduced from modeling studies based on predictions of rising temperatures and changing rainfall patterns. Here, we discuss the physiological responses of the coffee tree in the context of present and ongoing climate changes, including drought, heat, and light stresses, and interactions between these factors. We also summarize recent insights on the physiological and agronomic performance of coffee at elevated atmospheric CO2 concentrations and highlight the key role of CO2 in mitigating the harmful effects of heat stress. Evidence is shown suggesting that warming, per se, may be less harmful to coffee suitability than previously estimated, at least under the conditions of an adequate water supply. Finally, we discuss several mitigation strategies to improve crop performance in a changing world.}, number={21}, journal={Journal of Agricultural and Food Chemistry}, author={DaMatta, Fabio M and Avila, Rodrigo T and Cardoso, Amanda A and Martins, Samuel C V and Ramalho, Jose C}, year={2018}, pages={5264–5274} }
 @article{zhang_carins murphy_cardoso_jordan_brodribb_2018, title={Similar geometric rules govern the distribution of veins and stomata in petals, sepals and leaves}, volume={219}, url={https://publons.com/publon/3397443/}, DOI={10.1111/NPH.15210}, abstractNote={Investment in leaf veins (supplying xylem water) is balanced by stomatal abundance, such that sufficient water transport is provided for stomata to remain open when soil water is abundant. This coordination is mediated by a common dependence of vein and stomatal densities on cell size. Flowers may not conform to this same developmental pattern if they depend on water supplied by the phloem or have high rates of nonstomatal transpiration. We examined the relationships between veins, stomata and epidermal cells in leaves, sepals and petals of 27 angiosperms to determine whether common spacing rules applied to all tissues. Regression analysis found no evidence for different relationships within organ types. Both vein and stomatal densities were strongly associated with epidermal cell size within organs, but, for a given epidermal cell size, petals had fewer veins and stomata than sepals, which had fewer than leaves. Although our data support the concept of common scaling between veins and stomata in leaves and flowers, the large diversity in petal vein density suggests that, in some species, petal veins may be engaged in additional functions, such as the supply of water for high cuticular transpiration or for phloem delivery of water or carbohydrates.}, number={4}, journal={New Phytologist}, author={Zhang, Feng-Ping and Carins Murphy, Madeline R and Cardoso, Amanda A and Jordan, Gregory J and Brodribb, Timothy J}, year={2018}, pages={1224–1234} }
 @article{da-silva_canatto_cardoso_ribeiro_oliveira_2017, title={Arsenic-hyperaccumulation and antioxidant system in the aquatic macrophyte Spirodela intermedia W. Koch (Lemnaceae)}, volume={29}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-85039074965&partnerID=MN8TOARS}, DOI={10.1007/s40626-017-0096-8}, number={4}, journal={Theoretical and Experimental Plant Physiology}, publisher={Springer Nature}, author={da-Silva, Cristiane J. and Canatto, Regiane A. and Cardoso, Amanda A. and Ribeiro, Cleberson and Oliveira, Juraci A.}, year={2017}, pages={203–213} }
 @article{oliveira pires_souza_cardoso_santos dias_borges_2016, title={Action of nitric oxide in sesame seeds (Sesamum indicum L.) submitted to stress by cadmium,Ação protetora do óxido nítrico em sementes de gergelim (Sesamum indicum L.) submetidas ao estresse por cádmio}, volume={38}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84962751580&partnerID=MN8TOARS}, DOI={10.1590/2317-1545v38n1154824}, abstractNote={The objective of this paper was to evaluate the effect of nitric oxide (NO) as a protecting agent of sesame seeds submitted to different concentrations of cadmium. The treatments were: water (control), water increased by sodium nitroprusside (SNP) and other treatments regarding the concentrations of cadmium increased by SNP. The following determinations were done: germination, first count of germination, germination speed index, length of hypocotyl and radicle and dry matter of hypocotyl and radicle, besides quantification of enzyme activities, superoxide dismutase, catalase, ascorbate peroxidase and total peroxidases. The statistical design was entirely randomized with five replicates. The data was submitted to a variance analysis and the averages obtained for the treatments were compared by the Tukey test at 5% significance. The averages obtained in the treatments with and without SNP were compared by the F test at 5% probability. The NO due to the application of SNP was beneficial, providing an increase in germination, vigor and growth of seedlings. There was a progressive increase of the antioxidant enzymes activity in the period of 0 to 24 hours, showing an organization of the antioxidant system in the sesame seeds throughout germination time.}, number={1}, journal={Journal of Seed Science}, author={Oliveira Pires, Raquel Maria and Souza, Genaina Aparecida and Cardoso, Amanda Avila and Santos Dias, Denise Cunha and Borges, Eduardo Euclydes}, year={2016}, pages={22–29} }
 @article{pereira_correa_polo_castro_cardoso_pereira_2016, title={Seed germination of Schinus molle L. (Anacardiaceae) as related to its anatomy and dormancy alleviation}, volume={26}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84978168285&partnerID=MN8TOARS}, DOI={10.1017/S0960258516000167}, abstractNote={Abstract The seeds of Schinus molle are referred to as displaying physical dormancy because of their water-impermeable endocarp. Therefore, this work aimed to evaluate the germination of S. molle seeds as related to environmental conditions, scarification, storage time and seed anatomy. Various experiments were conducted to test the alleviation of dormancy in newly collected and stored seeds. Acid-scarified seeds incubated under continuous light at 25°C showed greatest vigour and germination. The separation of seeds by specific gravity revealed a higher germination percentage for those seeds that sank. In addition, dry storage alleviated dormancy with a remarkable increase in the various germination parameters. Overall, germination traits decreased after prolonged storage, but even after 12 months the means for germination parameters for stored seeds were still higher than those of newly collected ones. S. molle seeds remain attached to parts of the fruit mesocarp and endocarp. The mesocarp contains several layers of parenchyma showing secretory cavities. The endocarp consists of three layers of sclereids surrounding the embryo. Acid scarification strongly changed the structure of the external layers in the mesocarp, digesting parenchyma cells and removing the contents from both parenchyma cells and the secretory cavities; this improved water uptake during imbibition, which occurred only at the carpellary hilar slit. In conclusion, S. molle seeds are positively photoblastic and show physiological dormancy which can be alleviated by acid scarification and dry storage. Seeds can be stored for over 12 months without significant losses in germination parameters compared to newly collected seeds.}, number={4}, journal={Seed Science Research}, author={Pereira, Marcio P. and Correa, Felipe F. and Polo, Marcelo and Castro, Evaristo M. and Cardoso, Amanda A. and Pereira, Fabricio J.}, year={2016}, pages={351–361} }
 @article{cardoso_obolari_borges_silva_rodrigues_m. m. obolari_borges_silva_2015, title={Environmental factors on seed germination, seedling survival and initial growth of sacha inchi (Plukenetia volubilis l.),Fatores ambientais na germinação de sementes e na sobrevivência e crescimento inicial de plântulas de sacha inchi (Plukenetia volubilis L.)}, volume={37}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84937002825&partnerID=MN8TOARS}, DOI={10.1590/2317-1545v37n2145054}, abstractNote={Sacha inchi (Plukenetia volubilis L.) is an Amazon species of elevated agro-industrial potential due the high content of omega-3 and omega-6 in its seeds. Despite of it, little information about its propagation by seeds is currently available. Thus, the aim of this study was to assess seed germination, seedling survival and growth of this species under different conditions of substrate (on paper, between papers and paper roll), light (continuous darkness, 12-h photoperiod and continuous light) and temperature (continuous temperature at 20, 25, 30, 35 and 40 °C). Germination is stimulated by substrates with increased surface contact with the seeds, presence of light and temperatures between 25 and 35 °C. Survival and initial growth of seedlings are favored by vermiculite, continuous light and 30 °C temperature. These conditions allow rapid and uniform germination of seeds and better establishment and development of seedlings. We encourage the propagation of sacha inchi by seeds, since we consider it a feasible technique.}, number={2}, journal={Journal of Seed Science}, author={Cardoso, A. A. and Obolari, A. de M. M. and Borges, E. E. de and Silva, C. J. da and Rodrigues, H. S. and M. M. Obolari, A. and Borges, E. E. and Silva, C. J.}, year={2015}, pages={111–116} }
 @article{cardoso_borges_souza_silva_oliveira pires_santos dias_2015, title={Seed imbibition and germination of Plothymenia reticulata Benth. (Fabaceae) affected by mercury: Possible role of aquaporins}, volume={29}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84942324027&partnerID=MN8TOARS}, DOI={10.1590/0102-33062014abb0038}, abstractNote={Studies that evaluate the physiological and biochemical mechanisms of germination within forest species are needed in order to improve our understanding of such processes. Mercury and dithiothreitol are indicated as important tools in studies that assess the activity of aquaporins during imbibition and germination of seeds. To investigate the alterations caused by mercury inPlathymenia reticulata seedsdifferent doses of mercury were used in the presence and absence of dithiothreitol. Mercury had a dose-dependent effect on the seeds; in the most dilute solutions mercury partially inhibited the imbibition process, whereas in the most concentrated solutions it caused the death of the embryos. A delay in the hydration of the seeds may have caused decreased germination as a result of the reduced functionality of the aquaporins that were oxidized by mercury. In the presence of the reducing agent dithiothreitol, the activity of these proteins was restored and the germination process was re-established. These findings indicate the importance of aquaporins in the imbibition and germination stages of P. reticulataseeds, and they provide a better understanding of these important developmental events in plants.}, number={3}, journal={Acta Botanica Brasilica}, author={Cardoso, Amanda Avila and Borges, Eduardo Euclydes and Souza, Genaina Aparecida and Silva, Cristiane Jovelina and Oliveira Pires, Raquel Maria and Santos Dias, Denise Cunha}, year={2015}, pages={285–291} }
 @article{souza_oliveira_alvarenga_pires_pires_cardoso_o. pires_souza_oliveira_2014, title={Anatomical characteristics of rubber tree bark related to the production of natural rubber.}, volume={8}, url={http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=CABI&KeyUT=CABI:20143424408&KeyUID=CABI:20143424408}, number={16}, journal={Australian Journal of Basic and Applied Sciences}, author={Souza, G. A. de and Oliveira, L. E. M. de and Alvarenga, A. de P. and Pires, R. M. de O. and Pires, M. F. and Cardoso, A. A. and O. Pires, R. M. and Souza, G. A. and Oliveira, L. E. M.}, year={2014}, pages={79–84} }
 @article{anatomical characteristics of rubber tree bark related to the production of natural rubber._2014, url={https://publons.com/publon/15259783/}, journal={Australian Journal of Basic and Applied Sciences}, year={2014} }