Works (8)
Updated: July 5th, 2023 15:47
2019 journal article
Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(1).
author keywords: affinity chromatography; peptide ligand; antibody purification; binding capacity; process optimization; aggregate removal
TL;DR:
The use of peptide ligand HWRGWV and its cognate sequences to develop affinity adsorbents that compete with Protein A in terms of binding capacity and quality of the eluted product demonstrate the potential of peptides as alternative to Protein A for the purification of therapeutic antibodies.
(via Semantic Scholar)
open access via www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: March 11, 2019
2017 journal article
Design, selection, and development of cyclic peptide ligands for human erythropoietin
JOURNAL OF CHROMATOGRAPHY A, 1500, 105–120.
author keywords: Erythropoietin; Cyclic peptide ligands; Ligand screening; Affinity chromatography; Affinity maturation; In-silico docking
MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Erythropoietin / chemistry; Humans; Kinetics; Ligands; Molecular Sequence Data; Peptides, Cyclic / chemistry; Peptides, Cyclic / isolation & purification; Protein Binding
TL;DR:
Mutagenesis studies performed on FSLLSH with natural and non-natural amino acid substitutions led to the identification of critical EPO-binding determinants, and the discovery of new peptide ligands that facilitate EPO recovery.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2013 journal article
Reversible cyclic peptide libraries for the discovery of affinity ligands
Analytical Chemistry, 85(19), 9229–9237.
MeSH headings : Ligands; Molecular Structure; Peptide Library; Peptides, Cyclic / analysis; Tandem Mass Spectrometry
TL;DR:
A method is shown for synthesis of the irreversible cyclic peptide corresponding to the proposed depsipeptide structure, to make the ligand stable to the aqueous acid and alkaline conditions encountered in affinity chromatographic applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Alkaline-stable peptide ligand affinity adsorbents for the purification of biomolecules
Journal of Chromatography A, 1245, 55–64.
author keywords: Peptide ligand; Affinity adsorbent; NaOH stability; Modification of surface chemistry; Solid-phase peptide synthesis; Cleaning-in-place
MeSH headings : Adsorption; Alkalies / chemistry; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Ligands; Peptides / chemistry; Protein Binding
TL;DR:
It was found that the ligand leaching was caused by the coupling of a significant amount of peptide by alkaline-labile ester bonds, and the method presented has a broad application since it is valid for any peptide ligand identified for the purification of a biopharmaceutical target.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Peptide-based affinity adsorbents with high binding capacity for the purification of monoclonal antibodies
Industrial & Engineering Chemistry Research, 52(26), 8800–8811.
TL;DR:
The development of affinity resins based on hexapeptide ligand HWRGWV for recovering monoclonal antibodies from cell culture fluids and the reaction conditions of peptide coupling were optimized to maximize the binding capacity of the resulting adsorbents.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
Process for purification of monoclonal antibody expressed in transgenic Lemna plant extract using dextran-coated charcoal and hexamer peptide affinity resin
Journal of Chromatography A, 1260, 61–66.
author keywords: Dextran-coated charcoal; Transgenic plants; Hexapeptide ligand; IgG purification; Monoclonal antibody; Phenolics; Lemna minor
MeSH headings : Antibodies, Monoclonal / isolation & purification; Antibodies, Monoclonal / metabolism; Araceae / chemistry; Araceae / metabolism; Charcoal / chemistry; Chromatography, Affinity / methods; Dextrans / chemistry; Humans; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Peptides / chemistry; Peptides / metabolism; Plant Extracts / chemistry; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / metabolism; Protein Binding; Staphylococcal Protein A / chemistry; Staphylococcal Protein A / isolation & purification
TL;DR:
The development of a pretreatment and affinity separation process for the purification of a monoclonal antibody from transgenic Lemna plant extract and the performance of the hexamer peptide resin after the pretreatment step was found to be similar to that obtained with a commercial Protein A resin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2012 journal article
mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands
Biotechnology and Bioengineering, 110(3), 857–870.
author keywords: cyclic peptide; mRNA display; Fc binding; affinity ligand; library
MeSH headings : Humans; Immunoglobulin Fc Fragments / metabolism; Peptide Library; Peptides, Cyclic / chemistry; Peptides, Cyclic / genetics; Peptides, Cyclic / isolation & purification; Peptides, Cyclic / metabolism; Protein Binding; Protein Engineering / methods; RNA, Messenger / genetics; Solid-Phase Synthesis Techniques / methods
TL;DR:
The discovery, synthesis and characterization of novel cyclic peptide affinity ligands that bind the Fc portion of human Immunoglobulin G (IgG; hFc) are described and the approach can be extended to efficiently generate and evaluatecyclic peptides for other targets of interest.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2010 journal article
Performance of hexamer peptide ligands for affinity purification of immunoglobulin G from commercial cell culture media
Journal of Chromatography A, 1218(13), 1691–1700.
author keywords: Hexapeptide ligand; IgG purification; Monoclonal antibody; Affinity chromatography; Regeneration
MeSH headings : Adsorption; Animals; Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / isolation & purification; CHO Cells; Caprylates; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Culture Media; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Oligopeptides / chemistry; Phosphoric Acids; Protein Binding; Sodium Chloride; Urea
TL;DR:
Issues related to the successful application of the hexapeptide ligands, on a commercial methacrylate chromatographic resin, for the purification of IgG from mammalian cell culture fluids are addressed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
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