Amy Stieler Stewart

Also known as: A. Stieler; A. Stieler Stewart; A.S. Stewart; A.L. Stieler; A.L. Stieler Stewart

Works (19)

Updated: January 13th, 2025 14:20

2024 journal article

Assessment of equine intestinal epithelial junctional complexes and barrier permeability using a monolayer culture system

FRONTIERS IN VETERINARY SCIENCE, 11.

By: A. Stewart n , J. Kopper *, C. McKinney-Aguirre n , B. Veerasamy n, D. Sahoo *, J. Freund n, L. Gonzalez n 

author keywords: claudin; horse; jejunum; occludin; scratch assay; transepithelial resistance
Sources: Web Of Science, NC State University Libraries
Added: November 18, 2024

2023 journal article

45: Comparison of the Effects of Normothermic Machine Perfusion and Cold Storage Preservation on Porcine Intestinal Allograft Regenerative Potential and Viability

Transplantation, 107(7S), 25–25.

By: E. Ludwig n, N. Abraham *, C. McKinney  n, J. Freund n, A. Stewart n , K. Garman *, A. Barbas *, D. Sudan *, L. Gonzalez n 

Contributors: E. Ludwig n, N. Abraham *, C. McKinney  n, J. Freund n, A. Stewart n , K. Garman *, A. Barbas *, D. Sudan *, L. Gonzalez n 

Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: July 31, 2023

2023 journal article

A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease

FASEB JOURNAL, 37(6).

By: C. Schaaf n, K. Polkoff n, A. Carter n, A. Stewart n , B. Sheahan n , J. Freund n, J. Ginzel *, J. Snyder * ...

author keywords: APC mutation; colorectal cancer; intestine; Lgr5; porcine; stem cell; transgenic large animal
MeSH headings : Humans; Swine; Animals; Mice; In Situ Hybridization, Fluorescence; Intestines; Stem Cells; Ileum; Colon; Coleoptera; Green Fluorescent Proteins / genetics; Receptors, G-Protein-Coupled / genetics
TL;DR: This study validates ISC isolation in a new porcine Leucine Rich Repeat Containing G Protein‐Coupled Receptor 5 (LGR5) reporter line and demonstrates the use of these pigs as a novel colorectal cancer model. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: June 12, 2023

2023 journal article

Changes in equine intestinal stem/progenitor cell number at resection margins in cases of small intestinal strangulation

Equine Veterinary Journal, 55(6), 995–1002.

By: B. Veerasammy  n, G. Gonzalez n, P. Báez‐Ramos n, C. Schaaf n, A. Stewart n , E. Ludwig n, C. McKinney‐Aguirre, J. Freund n, J. Robertson n , L. Gonzalez n 

Contributors: B. Veerasammy  n, G. Gonzalez n, P. Baez-Ramos n, C. Schaaf n, A. Stewart n , E. Ludwig n, C. McKinney-Aguirre , J. Freund n, J. Robertson n , L. Gonzalez n 

author keywords: horse; immunofluorescence; intestine; Ki67; small intestinal strangulation; stem cell
TL;DR: Proliferating cell and ISC numbers may be associated with case outcome, and associations between biomarker expression and the number of preoperative risk factors and postoperative complications negatively related to outcome were determined. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries, Crossref
Added: January 31, 2023

2023 journal article

Comparison of the effects of normothermic machine perfusion and cold storage preservation on porcine intestinal allograft regenerative potential and viability

American Journal of Transplantation, 24(4), 564–576.

By: E. Ludwig n, N. Abraham *, C. Schaaf n, C. McKinney  n, J. Freund n, A. Stewart n , B. Veerasammy  n, M. Thomas  n ...

Contributors: E. Ludwig n, N. Abraham *, C. Schaaf n, C. McKinney  n, J. Freund n, A. Stewart n , B. Veerasammy  n, M. Thomas  n ...

author keywords: intestinal transplantation; normothermic machine perfusion; cold storage; ischemia-reperfusion injury; intestinal stem cells; preservation injury
TL;DR: Normothermic machine perfusion preservation of kidney and liver allografts minimizes CS-associated ischemia-reperfusion injury and appears to reduce IRI and improve graft regeneration with improved ISC viability and proliferation. (via Semantic Scholar)
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Sources: ORCID, Web Of Science, NC State University Libraries, Crossref
Added: December 28, 2023

2022 journal article

Culture of equine intestinal epithelial stem cells after delayed tissue storage for future applications

BMC VETERINARY RESEARCH, 18(1).

By: A. Stewart n , C. Schaaf n, B. Veerasammy  n, J. Freund n & L. Gonzalez n 

author keywords: Horse; Stem cell; 3D culture; Organoid; Enteroid; Intestine; Storage
MeSH headings : Animals; Cryopreservation / veterinary; Epithelial Cells / cytology; Horses; Intestines / cytology; Stem Cells / cytology; Time Factors; Cells, Cultured; Intestinal Diseases / therapy; Intestinal Diseases / veterinary
TL;DR: Equine ISCs can be isolated and cultured after prolonged tissue storage and this suggests that ISCs could be isolated for several days from samples properly stored after procedures, including surgery or necropsy, and used to create ISC repositories for study or therapy of equine intestinal diseases. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 30, 2023

2022 journal article

Orthotopic Transplantation of the Full-length Porcine Intestine After Normothermic Machine Perfusion

Transplantation Direct, 8(11), e1390.

By: N. Abraham *, E. Ludwig n, C. Schaaf n, B. Veerasammy  n, A. Stewart n , C. McKinney  n, J. Freund n, J. Brassil ...

Contributors: N. Abraham *, E. Ludwig n, C. Schaaf n, B. Veerasammy  n, A. Stewart n , C. McKinney  n, J. Freund n, J. Brassil ...

TL;DR: Development and optimization of machine perfusion preservation of small intestine and successful transplantation of intestinal allografts in a porcine model are reported. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: October 31, 2022

2021 journal article

HOPX+ injury-resistant intestinal stem cells drive epithelial recovery after severe intestinal ischemia

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 321(5), G588–G602.

By: A. Stewart n , C. Schaaf n, J. Luff n, J. Freund n, T. Becker *, S. Tufts n, J. Robertson n , L. Gonzalez n 

author keywords: epithelial repair; HOPX; ischemia; large animal models; stem cell
MeSH headings : Animals; Cell Proliferation; Disease Models, Animal; Epithelial Cells / metabolism; Epithelial Cells / pathology; Female; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Intestinal Mucosa / metabolism; Intestinal Mucosa / pathology; Male; Mesenteric Ischemia / genetics; Mesenteric Ischemia / metabolism; Mesenteric Ischemia / pathology; Phenotype; Re-Epithelialization; Severity of Illness Index; Stem Cells / metabolism; Stem Cells / pathology; Sus scrofa; Tissue Culture Techniques
TL;DR: It is demonstrated that during early in vivo recovery, injury-resistant HOPX+cells maintain quiescence, suggesting that HopX may serve a functional role in ISC mediated regeneration after injury and could be a target to control ISC proliferation. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: November 3, 2021

2020 journal article

Geographic Disparities in Clinical Characteristics of Duodenitis–Proximal Jejunitis in Horses in the United States

Journal of Equine Veterinary Science, 93, 103192.

By: S. Steward *, D. Hassel *, H. Martin*, C. Doddman*, A. Stewart* , E. Elzer *, L. Southwood *

author keywords: Gastrointestinal; Small intestine; Duodenitis-proximal jejunitis; Colic
MeSH headings : Animals; Case-Control Studies; Duodenitis / epidemiology; Duodenitis / veterinary; Horse Diseases / epidemiology; Horses; Jejunal Diseases / veterinary; Prospective Studies; Southeastern United States; United States / epidemiology
TL;DR: Differences in clinical parameters of horses with DPJ between geographic regions were identified to corroborate anecdotal reports and support theories of differing etiologies and identify risk factors contributing toward regional differences in this disease. (via Semantic Scholar)
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Source: ORCID
Added: July 16, 2020

2019 journal article

Preservation of reserve intestinal epithelial stem cells following severe ischemic injury

American Journal of Physiology-Gastrointestinal and Liver Physiology, 316(4), G482–G494.

By: L. Gonzalez n , A. Stewart n , J. Freund  n, C. Kucera n, C. Dekaney  n, S. Magness  n, A. Blikslager n 

author keywords: intestine; ischemia reperfusion injury; large animal model; reserve stem cells; stem cell
MeSH headings : Animals; Apoptosis; Cell Proliferation / physiology; Cell Self Renewal / physiology; Cell Survival / physiology; Disease Models, Animal; Homeodomain Proteins / metabolism; Intestinal Mucosa / blood supply; Intestinal Mucosa / metabolism; Intestinal Mucosa / pathology; Ischemia / metabolism; Receptors, G-Protein-Coupled / metabolism; Reperfusion Injury / metabolism; Swine
TL;DR: Data indicate that Hopx+ cells are resistant to injury and are the likely source of epithelial renewal following prolonged ischemic injury, and it is therefore possible that targeting reserve stem cells will lead to new therapies for patients with severe intestinal injury. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: March 16, 2019

2018 journal article

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Journal of Visualized Experiments, 5(135).

By: A. Stieler Stewart  n, J. Freund n, A. Blikslager n  & L. Gonzalez n 

author keywords: Medicine; Issue 135; Porcine; intestine; ischemia-reperfusion; stem cell; enteroid; organoid
MeSH headings : Animals; Cell Culture Techniques / methods; Disease Models, Animal; Humans; Intestine, Small / cytology; Intestine, Small / metabolism; Intestine, Small / pathology; Ischemia / etiology; Mice; Stem Cells / metabolism; Swine
TL;DR: By utilizing a porcine model, a protocol is established in which segmental loops of intestinal ischemia can be created within a single animal, enabling the study of differing time points of ischemic injury and repair in vivo. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Advanced three-dimensional culture of equine intestinal epithelial stem cells

Equine Veterinary Journal, 50(2), 241–248.

By: A. Stewart  n, J. Freund n & L. Gonzalez n 

author keywords: horse; stem cell; 3D culture; intestine; enteroid; organoid
MeSH headings : Animals; Cell Culture Techniques / veterinary; Cell Differentiation; Female; Horses; Intestinal Mucosa / cytology; Male; Models, Biological; Stem Cells / physiology
TL;DR: The successful culture of equine intestinal crypts into 3D "mini-guts" allows for in vitro studies of the equine intestine and has relevance to future development of novel therapies that harness the regenerative potential ofequine intestine in horses with gastrointestinal disease (colic). (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2017 review

Alterations in Intestinal Permeability: The Role of the "Leaky Gut" in Health and Disease

[Review of ]. JOURNAL OF EQUINE VETERINARY SCIENCE, 52, 10–22.

By: A. Stewart n , S. Pratt-Phillips  n & L. Gonzalez n 

author keywords: Horse; Intestinal permeability; Leaky gut; Barrier function; Decreased performance
TL;DR: The normal intestinal barrier, measurement of barrier permeability, pathogenesis and main causes of altered permeability are reviewed, and potential alternative therapies of leaky gut in horses are highlighted while relating what has been studied in other species. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2017 journal article

Effects of clarithromycin, azithromycin and rifampicin on terbutaline-induced sweating in foals

Equine Veterinary Journal, 3.

By: A. Stewart *, L. Sanchez *, M. Mallicote *, A. Muniz*, M. Westerterp *, J. Burrow*, R. MacKAY *

author keywords: horse; foal; anhidrosis; rifampicin; macrolide; Rhodococcus equi
MeSH headings : Animals; Azithromycin / pharmacology; Clarithromycin / pharmacology; Horses; Rifampin / pharmacology; Sweating / drug effects; Terbutaline
TL;DR: The potent anti-sudorific effect of ERY is delayed, but not substantially affected by concurrent RIF administration, and effects of RIF on ERY-induced anhidrosis could not definitively be ascribed to altered ERY bioavailability. (via Semantic Scholar)
Source: ORCID
Added: February 11, 2019

2016 journal article

Spinal Cord Hamartomatous Myelodysplasia in 2 Horses With Clinical Neurologic Deficits

Veterinary Pathology, 53(4), 844–846.

By: K. Taylor *, R. MacKay *, E. Nelson *, A. Stieler*, J. Roberts & W. Castleman *

author keywords: ataxia; hamartoma; horse; hydromyelia; muscle spasm; myelodysplasia; spinal cord
MeSH headings : Animals; Ataxia / pathology; Ataxia / veterinary; Hamartoma / diagnosis; Hamartoma / pathology; Hamartoma / veterinary; Hindlimb / pathology; Horse Diseases / diagnosis; Horse Diseases / pathology; Horses; Neural Tube Defects / diagnosis; Neural Tube Defects / pathology; Neural Tube Defects / veterinary; Spasm / pathology; Spasm / veterinary; Spinal Cord / pathology
TL;DR: Two horses euthanized for neurologic deficits were diagnosed with hamartomatous myelodysplasia of the spinal cord, which had focal disorganization of the white and gray matter of the cerebral spinal cord forming a mass interspersed with neurons, glial cells, and disoriented axon bundles. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
Source: ORCID
Added: February 11, 2019

2015 journal article

Macrolide-induced hyperthermia in foals: Role of impaired sweat responses

Equine Veterinary Journal, 48(5), 590–594.

By: A. Stieler*, L. Sanchez *, M. Mallicote *, B. Martabano *, J. Burrow* & R. MacKay *

author keywords: horse; anhidrosis; erythromycin; hyperthermia; foal; sweat
MeSH headings : Animals; Anti-Bacterial Agents / adverse effects; Erythromycin / adverse effects; Female; Fever / chemically induced; Fever / veterinary; Horse Diseases / chemically induced; Horses; Injections, Intradermal; Intradermal Tests; Male; Sweating / drug effects; Sympathomimetics / administration & dosage; Sympathomimetics / pharmacology; Terbutaline / administration & dosage; Terbutaline / pharmacology
TL;DR: Drug-induced anhidrosis is the likely cause of hyperthermia in some foals treated with erythromycin, and terbutaline-induced sweating was significantly reduced from baseline in eryhromycin-treated foals at all subsequent days. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: February 11, 2019

2014 journal article

Non-Surgical management of vaginal prolapse in a late gestation alpaca (Lamos pacos)

Clinical Theriogenology, 6(4), 489–493.

By: J. McNaughten, M. Pozor, M. Mallicote, A. Stieler, A. Kelleman & M. MacPherson

Source: NC State University Libraries
Added: February 18, 2019

2013 journal article

What Is Your Neurologic Diagnosis?

Journal of the American Veterinary Medical Association, 243(6), 779–781.

By: A. Stieler*, S. Reuss, N. Werpy & R. MacKay

MeSH headings : Animals; Female; Horse Diseases / diagnosis; Horse Diseases / parasitology; Horse Diseases / pathology; Horses; Radiography; Sarcocystis / immunology; Sarcocystosis / diagnosis; Sarcocystosis / diagnostic imaging; Sarcocystosis / pathology; Sarcocystosis / veterinary
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3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: February 11, 2019

2012 journal article

Neutrophil and monocyte function in neonatal dairy calves fed fresh or frozen colostrum

International Journal of Applied Research in Veterinary Medicine, 10(4), 328–334.

By: A. Stieler, B. Bernardo & G. Donovan

Source: NC State University Libraries
Added: February 18, 2019

Employment

Updated: October 16th, 2024 23:32

2023 - present

North Carolina State University Raleigh, US
Assistant Teaching Professor, Physiology Molecular Biomedical Sciences

2019 - 2022

NC State University Raleigh, NC, US
Assistant Research Professor Department of Clinical Sciences

Education

Updated: April 4th, 2020 13:09

2015 - 2019

North Carolina State University Raleigh, NC, US
PhD Comparative Biomedical Sciences

2012 - 2015

University of Florida College of Veterinary Medicine Gainesville, FL, US
Resident, Large Animal Internal Medicine (DACVIM)

2011 - 2012

University of Georgia College of Veterinary Medicine Athens, GA, US
Internship, Large Animal Medicine and Surgery

2007 - 2011

University of Florida College of Veterinary Medicine Gainesville, FL, US
DVM

2003 - 2007

University of Florida Gainesville, FL, US
BS/Animal Biology

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