@article{taylor_hamby_deyonke_gould_fritz_2021, title={Genome evolution in an agricultural pest following adoption of transgenic crops}, volume={118}, ISSN={["1091-6490"]}, DOI={10.1073/pnas.2020853118}, abstractNote={SignificanceEvolution of resistance to management approaches in agricultural landscapes is common and results in economic losses. Early detection of pest resistance prior to significant crop damage would benefit the agricultural community. It has been hypothesized that new genomic approaches could track molecular signals of emerging resistance and trigger efforts to preempt widespread damage. We tested this hypothesis by quantifying genomic changes in the pestHelicoverpa zeaover a 15-y period concurrent with commercialization of transgenicBacillus thuringiensis–expressing crops and their subsequent loss of efficacy. Our results demonstrate the complex nature of evolution in agricultural ecosystems and provide insight into the potential and pitfalls of using genomic approaches for resistance monitoring.}, number={52}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Taylor, Katherine L. and Hamby, Kelly A. and DeYonke, Alexandra M. and Gould, Fred and Fritz, Megan L.}, year={2021}, month={Dec} }
 @article{fritz_deyonke_papanicolaou_micinski_westbrook_gould_2018, title={Contemporary evolution of a Lepidopteran species, Heliothis virescens, in response to modern agricultural practices}, volume={27}, ISSN={["1365-294X"]}, DOI={10.1111/mec.14430}, abstractNote={AbstractAdaptation to human‐induced environmental change has the potential to profoundly influence the genomic architecture of affected species. This is particularly true in agricultural ecosystems, where anthropogenic selection pressure is strong. Heliothis virescens primarily feeds on cotton in its larval stages, and US populations have been declining since the widespread planting of transgenic cotton, which endogenously expresses proteins derived from Bacillus thuringiensis (Bt). No physiological adaptation to Bt toxin has been found in the field, so adaptation in this altered environment could involve (i) shifts in host plant selection mechanisms to avoid cotton, (ii) changes in detoxification mechanisms required for cotton‐feeding vs. feeding on other hosts or (iii) loss of resistance to previously used management practices including insecticides. Here, we begin to address whether such changes occurred in H. virescens populations between 1997 and 2012, as Bt‐cotton cultivation spread through the agricultural landscape. For our study, we produced an H. virescens genome assembly and used this in concert with a ddRAD‐seq‐enabled genome scan to identify loci with significant allele frequency changes over the 15‐year period. Genetic changes at a previously described H. virescens insecticide target of selection were detectable in our genome scan and increased our confidence in this methodology. Additional loci were also detected as being under selection, and we quantified the selection strength required to elicit observed allele frequency changes at each locus. Potential contributions of genes near loci under selection to adaptive phenotypes in the H. virescens cotton system are discussed.}, number={1}, journal={MOLECULAR ECOLOGY}, author={Fritz, Megan L. and DeYonke, Alexandra M. and Papanicolaou, Alexie and Micinski, Stephen and Westbrook, John and Gould, Fred}, year={2018}, month={Jan}, pages={167–181} }