@article{casoni_benedusi_cervellati_pecorelli_ferrara_vallese_valacchi_2023, title={CADMIUM EXPOSURE AFFECTS THE PROGRESSION OF HUMAN METASTATIC MELANOMA BY INDUCING A DEREGULATION OF ANTIOXIDANT CELL RESPONSE}, volume={201}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2023.03.174}, DOI={10.1016/j.freeradbiomed.2023.03.174}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Casoni, Alice and Benedusi, Mascia and Cervellati, Franco and Pecorelli, Alessandra and Ferrara, Francesca and Vallese, Andrea and Valacchi, Giuseppe}, year={2023}, month={May}, pages={42–43} }
 @article{ivarsson_ferrara_vallese_guiotto_colella_pecorelli_valacchi_2023, title={Comparison of Pollutant Effects on Cutaneous Inflammasomes Activation}, volume={24}, ISSN={["1422-0067"]}, url={https://doi.org/10.3390/ijms242316674}, DOI={10.3390/ijms242316674}, abstractNote={The skin is the outermost layer of the body and, therefore, is exposed to a variety of stressors, such as environmental pollutants, known to cause oxinflammatory reactions involved in the exacerbation of several skin conditions. Today, inflammasomes are recognized as important modulators of the cutaneous inflammatory status in response to air pollutants and ultraviolet (UV) light exposure. In this study, human skin explants were exposed to the best-recognized air pollutants, such as microplastics (MP), cigarette smoke (CS), diesel engine exhaust (DEE), ozone (O3), and UV, for 1 or 4 days, to explore how each pollutant can differently modulate markers of cutaneous oxinflammation. Exposure to environmental pollutants caused an altered oxidative stress response, accompanied by increased DNA damage and signs of premature skin aging. The effect of specific pollutants being able to exert different inflammasomes pathways (NLRP1, NLRP3, NLRP6, and NLRC4) was also investigated in terms of scaffold formation and cell pyroptosis. Among all environmental pollutants, O3, MP, and UV represented the main pollutants affecting cutaneous redox homeostasis; of note, the NLRP1 and NLRP6 inflammasomes were the main ones modulated by these outdoor stressors, suggesting their role as possible molecular targets in preventing skin disorders and the inflammaging events associated with environmental pollutant exposure.}, number={23}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Ivarsson, John and Ferrara, Francesca and Vallese, Andrea and Guiotto, Anna and Colella, Sante and Pecorelli, Alessandra and Valacchi, Giuseppe}, year={2023}, month={Dec} }
 @article{cordone_vallese_pecorelli_guiotto_ferrara_benedusi_cervellati_hayek_valacchi_2023, title={Deregulated NLRP3 inflammasome response due to a constitutive activation of NF-kappa B p65 pro-inflammatory pathway in lymphomonocytes of Rett syndrome patients}, volume={198}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2022.12.075}, DOI={10.1016/j.freeradbiomed.2022.12.075}, abstractNote={Elevated levels of the type III (III) isoforms of neuregulin 1 (NRG1) have been observed in the brains of schizophrenia patients that carry NRG1 HapICE risk alleles, which is thought to contribute to the aetiology of the disease. We generated transgenic mice with forebrain driven Nrg1 III overexpression (Nrg1 III tg) and previously found that male heterozygous Nrg1 type III tg mice exhibit several schizophrenia-relevant behaviours including social and cognitive deficits as well as impaired sensorimotor gating. A number of mouse models for other Nrg1 isoform types exhibit sex-specific phenotypes yet sex-specific effects of Nrg1 III overexpression had not been evaluated. Thus, in this study we tested female Nrg1 III transgenic mice using a comprehensive behavioural phenotyping battery relevant to positive, negative and cognitive symptoms of schizophrenia. Firstly, forebrain Nrg1 III mRNA overexpression was confirmed in female transgenic mice using by qPCR. In the open field test, female Nrg1 III mice exhibited a blunted response to an acute challenge with the N-methyl-d-aspartate (NMDA) receptor antagonist MK-801. Female Nrg1 III tg mice also exhibited moderately impaired short-term memory. Other behavioural domains including sensory abilities, motor functions, baseline locomotion, anxiety, sociability, social recognition memory, fear conditioning and prepulse inhibition were unperturbed in Nrg1 III tg females. Together these results illustrate that overexpressing forebrain Nrg1 III in female mice modifies the locomotive response to NMDA receptor antagonism without causing severe alterations to a number of other schizophrenia-related behavioural domains. The data suggest that behavioural effects of Nrg1 III overexpression may be sex-dependent.}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Cordone, Valeria and Vallese, Andrea and Pecorelli, Alessandra and Guiotto, Anna and Ferrara, Francesca and Benedusi, Mascia and Cervellati, Franco and Hayek, Joussef and Valacchi, Giuseppe}, year={2023}, month={Mar}, pages={S21–S22} }
 @article{ferrara_pecorelli_guiotto_vallese_cordone_benedusi_cervellati_valacchi_2023, title={INFLAMMASOMES REGULATION BY ENVIRONMENTAL POLLUTANTS IN HUMAN SKIN}, volume={201}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2023.03.158}, DOI={10.1016/j.freeradbiomed.2023.03.158}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Ferrara, Francesca and Pecorelli, Alessandra and Guiotto, Anna and Vallese, Andrea and Cordone, Valeria and Benedusi, Mascia and Cervellati, Franco and Valacchi, Giuseppe}, year={2023}, month={May}, pages={38–39} }
 @article{vallese_pecorelli_cordone_ferrara_benedusi_cervellati_hayek_valacchi_2023, title={MITOCHONDRIAL DYSFUNCTION AND NLRP3 INFLAMMASOME ACTIVATION IN AUTISM SPECTRUM DISORDER}, volume={201}, ISSN={["1873-4596"]}, DOI={10.1016/j.freeradbiomed.2023.03.094}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Vallese, Andrea and Pecorelli, Alessandra and Cordone, Valeria and Ferrara, Francesca and Benedusi, Mascia and Cervellati, Franco and Hayek, Joussef and Valacchi, Giuseppe}, year={2023}, month={May}, pages={20–20} }
 @article{vallese_cordone_pecorelli_valacchi_2023, title={Ox-inflammasome involvement in neuroinflammation}, volume={207}, ISSN={["1873-4596"]}, DOI={10.1016/j.freeradbiomed.2023.07.010}, abstractNote={Neuroinflammation plays a crucial role in the onset and the progression of several neuropathologies, from neurodegenerative disorders to migraine, from Rett syndrome to post-COVID 19 neurological manifestations. Inflammasomes are cytosolic multiprotein complexes of the innate immune system that fuel inflammation. They have been under study for the last twenty years and more recently their involvement in neuro-related conditions has been of great interest as possible therapeutic target. The role of oxidative stress in inflammasome activation has been described, however the exact way of action of specific endogenous and exogenous oxidants needs to be better clarified. In this review, we provide the current knowledge on the involvement of inflammasome in the main neuropathologies, emphasizing the importance to further clarify the role of oxidative stress in its activation including the role of mitochondria in inflammasome-induced neuroinflammation.}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Vallese, Andrea and Cordone, Valeria and Pecorelli, Alessandra and Valacchi, Giuseppe}, year={2023}, month={Oct}, pages={161–177} }
 @article{silvestri_marcheggiani_orlando_cirilli_mengarelli_vallese_pecorelli_valacchi_tiano_2023, title={ROLE OF COENZYME Q10 IN MITOCHONDRIAL DYSFUNCTION AND OXIDATIVE DAMAGE IN RETT SYNDROME}, volume={201}, ISSN={["1873-4596"]}, DOI={10.1016/j.freeradbiomed.2023.03.080}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Silvestri, Sonia and Marcheggiani, Fabio and Orlando, Patrick and Cirilli, Ilenia and Mengarelli, Francesco and Vallese, Andrea and Pecorelli, Alessandra and Valacchi, Giuseppe and Tiano, Luca}, year={2023}, month={May}, pages={16–16} }
 @article{benedusi_canella_vallese_casoni_guerra_rispoli_ferrara_cervellati_valacchi_2023, title={THE POSSIBLE MODULATION OF NRF2 AND NF-KB CROSSTALK BY SULFORAPHANE IN THE PROGRESSION OF HUMAN MELANOMA}, volume={201}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2023.03.230}, DOI={10.1016/j.freeradbiomed.2023.03.230}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Benedusi, Mascia and Canella, Rita and Vallese, Andrea and Casoni, Alice and Guerra, Martina and Rispoli, Giorgio and Ferrara, Francesca and Cervellati, Franco and Valacchi, Giuseppe}, year={2023}, month={May}, pages={57–57} }
 @article{vallese_pecorelli_cordone_ferrara_benedusi_cervellati_hayek_valacchi_2023, title={The pathogenic axis between mitochondrial dysfunction and NLRP3 inflammasome activation in Autism Spectrum Disorder}, volume={208}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2023.10.112}, DOI={10.1016/j.freeradbiomed.2023.10.112}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, author={Vallese, Andrea and Pecorelli, Alessandra and Cordone, Valeria and Ferrara, Francesca and Benedusi, Mascia and Cervellati, Franco and Hayek, Joussef and Valacchi, Giuseppe}, year={2023}, month={Nov}, pages={S49–S49} }
 @article{canella_benedusi_vallese_pecorelli_guiotto_ferrara_rispoli_cervellati_valacchi_2023, title={The role of potassium current in the pulmonary response to environmental oxidative stress}, volume={2}, url={http://dx.doi.org/10.1016/j.abb.2023.109534}, DOI={10.1016/j.abb.2023.109534}, abstractNote={Exposure of human lung epithelial cells (A549 cell line) to the oxidant pollutant ozone (O3) alters cell membrane currents inducing its decrease, when the cell undergoes to a voltage-clamp protocol ranging from -90 to +70mV. The membrane potential of these cells is mainly maintained by the interplay of potassium and chloride currents. Our previous studies indicated the ability of O3 to activate ORCC (Outward Rectifier Chloride Channel) and consequently increases the chloride current. In this paper our aim was to understand the response of potassium current to oxidative stress challenge and to identify the kind potassium channel involved in O3 induced current changes. After measuring the total membrane current using an intracellular solution with or without potassium ions, we obtained the contribution of potassium to the overall membrane current in control condition by a mathematical approach. Repeating these experiments after O3 treatment we observed a significant decrease of Ipotassium. Treatment of the cells with Iberiotoxin (IbTx), a specific inhibitor of BK channel, we were able to verify the presence and the functionality of BK channels. In addition, the administration of 4-Aminopyridine (an inhibitor of voltage dependent K channels but not BK channels) and Tetraethylammonium (TEA) before and after O3 treatment we observed the formation of BK oxidative post-translation modifications. Our data suggest that O3 is able to inhibit potassium current by targeting BK channel. Further studies are needed to better clarify the role of this BK channel and its interplay with the other membrane channels under oxidative stress conditions. These findings can contribute to identify the biomolecular pathway induced by O3 allowing a possible pharmacological intervention against oxidative stress damage in lung tissue.}, journal={Archives of Biochemistry and Biophysics}, publisher={Elsevier BV}, author={Canella, Rita and Benedusi, Mascia and Vallese, Andrea and Pecorelli, Alessandra and Guiotto, Anna and Ferrara, Francesca and Rispoli, Giorgio and Cervellati, Franco and Valacchi, Giuseppe}, year={2023}, month={Feb}, pages={109534} }
 @article{pecorelli_ferrara_guiotto_vallese_cordone_belmonte_hayek_cervellati_valacchi_2022, title={Loss of Scavenger Receptor B1 (SR-B1) in Brain of a Rett Syndrome Mouse Model}, volume={192}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2022.10.159}, DOI={10.1016/j.freeradbiomed.2022.10.159}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, publisher={Elsevier BV}, author={Pecorelli, Alessandra and Ferrara, Francesca and Guiotto, Anna and Vallese, Andrea and Cordone, Valeria and Belmonte, Giuseppe and Hayek, Joussef and Cervellati, Carlo and Valacchi, Giuseppe}, year={2022}, month={Nov} }
 @article{ferrara_cordone_pecorelli_benedusi_pambianchi_guiotto_vallese_cervellati_valacchi_2022, title={Ubiquitination as a key regulatory mechanism for O-3-induced cutaneous redox inflammasome activation}, volume={56}, ISSN={["2213-2317"]}, url={http://dx.doi.org/10.1016/j.redox.2022.102440}, DOI={10.1016/j.redox.2022.102440}, abstractNote={NLRP1 is one of the major inflammasomes modulating the cutaneous inflammatory responses and therefore linked to a variety of cutaneous conditions. Although NLRP1 has been the first inflammasome to be discovered, only in the past years a significant progress was achieved in understanding the molecular mechanism and the stimuli behind its activation. In the past decades a crescent number of studies have highlighted the role of air pollutants as Particulate Matter (PM), Cigarette Smoke (CS) and Ozone (O3) as trigger stimuli for inflammasomes activation, especially via Reactive Oxygen Species (ROS) mediators. However, whether NLRP1 can be modulated by air pollutants via oxidative stress and the mechanism behind its activation is still poorly understood. Here we report for the first time that O3, one of the most toxic pollutants, activates the NLRP1 inflammasome in human keratinocytes via oxidative stress mediators as hydrogen peroxide (H2O2) and 4-hydroxy-nonenal (4HNE). Our data suggest that NLRP1 represents a target protein for 4HNE adduction that possibly leads to its proteasomal degradation and activation via the possible involvement of E3 ubiquitin ligase UBR2. Of note, Catalase (Cat) treatment prevented inflammasome assemble and inflammatory cytokines release as well as NLRP1 ubiquitination in human keratinocytes upon O3 exposure. The present work is a mechanistic study that follows our previous work where we have showed the ability of O3 to induce cutaneous inflammasome activation in humans exposed to this pollutant. In conclusion, our results suggest that O3 triggers the cutaneous NLRP1 inflammasome activation by ubiquitination and redox mechanism.}, journal={REDOX BIOLOGY}, publisher={Elsevier BV}, author={Ferrara, Francesca and Cordone, Valeria and Pecorelli, Alessandra and Benedusi, Mascia and Pambianchi, Erika and Guiotto, Anna and Vallese, Andrea and Cervellati, Franco and Valacchi, Giuseppe}, year={2022}, month={Oct} }
 @article{ferrara_cordone_pecorelli_benedusi_pambianchi_guiotto_vallese_cervellati_valacchi_2022, title={Ubiquitination as a key regulatory mechanism for O3-induced cutaneous redox Inflammasome activation}, volume={192}, ISSN={["1873-4596"]}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2022.10.033}, DOI={10.1016/j.freeradbiomed.2022.10.033}, abstractNote={Redox signaling is an important emerging mechanism of cellular function. Dysfunctional redox signaling is increasingly implicated in numerous pathologies, including atherosclerosis, diabetes, and cancer. The molecular messengers in this type of signaling are reactive species which can mediate the post-translational modification of specific groups of proteins, thereby effecting functional changes in the modified proteins. Electrophilic compounds comprise one class of reactive species which can participate in redox signaling. Electrophiles modulate cell function via formation of covalent adducts with proteins, particularly cysteine residues.This review will discuss the commonly used methods of detection for electrophile-sensitive proteins, and will highlight the importance of identifying these proteins for studying redox signaling and developing novel therapeutics.There are several methods which can be used to detect electrophile-sensitive proteins. These include the use of tagged model electrophiles, as well as derivatization of endogenous electrophile–protein adducts.In order to understand the mechanisms by which electrophiles mediate redox signaling, it is necessary to identify electrophile-sensitive proteins and quantitatively assess adduct formation. Strengths and limitations of these methods will be discussed. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn.}, journal={FREE RADICAL BIOLOGY AND MEDICINE}, publisher={Elsevier BV}, author={Ferrara, Francesca and Cordone, Valeria and Pecorelli, Alessandra and Benedusi, Mascia and Pambianchi, Erika and Guiotto, Anna and Vallese, Andrea and Cervellati, Franco and Valacchi, Giuseppe}, year={2022}, month={Nov} }
 @inproceedings{guiotto_cordone_vallese_benedusi_cervellati_hayek_cervellati_valacchi_pecorelli_2022, title={[YIA] Evidence of ferroptosis involvement in Rett syndrome pathogenesis}, volume={189}, url={http://dx.doi.org/10.1016/j.freeradbiomed.2022.06.076}, DOI={10.1016/j.freeradbiomed.2022.06.076}, booktitle={Free Radical Biology and Medicine}, publisher={Elsevier BV}, author={Guiotto, Anna and Cordone, Valeria and Vallese, Andrea and Benedusi, Mascia and Cervellati, Franco and Hayek, Joussef and Cervellati, Carlo and Valacchi, Giuseppe and Pecorelli, Alessandra}, year={2022}, month={Aug}, pages={15} }