Brandyn David Moore

College of Engineering

Works (9)

Updated: June 14th, 2025 05:01

2025 journal article

Light-Controlled Peptide Ligands for the Photoaffinity Purification of FLT3 and CD38

ACS Applied Engineering Materials, 3(2), 379–391.

By: B. Moore n, R. Prodromou n, G. Rusch n, C. Nguyen n, W. Chu n, V. Pozdin*, M. Daniele n , S. Menegatti n 

Contributors: B. Moore n, R. Prodromou n, G. Rusch n, C. Nguyen n, W. Chu n, V. Pozdin*, M. Daniele n , S. Menegatti n 

author keywords: azobenzene-cyclic peptides; affinity ligands; photoaffinity chromatography; biorecognition; cellmarkers
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Semiconductor materials and devices; Oxidative Organic Chemistry Reactions
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 10, 2025

2025 article

Purification of CRISPR Cas12a from E. coli cell lysates using peptide affinity ligands

Wu, Y., Kilgore, R., Hetzler, Z., Chu, W., Moore, B., Barbieri, E., … Menegatti, S. (2025, May 17). Journal of Chromatography A, Vol. 1755.

By: Y. Wu n, R. Kilgore n, Z. Hetzler n, W. Chu n, B. Moore n, E. Barbieri n, Q. Wei n , S. Menegatti n 

author keywords: CRISPR Cas12a; Gene editing; Peptide ligands; Affinity chromatography; Protein purification
MeSH headings : Escherichia coli / chemistry; Escherichia coli / genetics; Escherichia coli / enzymology; Chromatography, Affinity / methods; Ligands; CRISPR-Associated Proteins / isolation & purification; CRISPR-Associated Proteins / metabolism; CRISPR-Associated Proteins / chemistry; Peptides / chemistry; Peptides / metabolism; CRISPR-Cas Systems; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Bacterial Proteins / chemistry; Endodeoxyribonucleases / isolation & purification; Endodeoxyribonucleases / metabolism; Endodeoxyribonucleases / chemistry; Acidaminococcus / enzymology
topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Advanced biosensing and bioanalysis techniques
Sources: Web Of Science, NC State University Libraries
Added: June 9, 2025

2024 journal article

Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates

Journal of Chromatography A, 1736, 465396.

By: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n , S. Menegatti n 

author keywords: Affinity chromatography; Adenovirus; Viral vectors; Gene therapy; Peptide ligands
MeSH headings : Humans; Chromatography, Affinity / methods; Chlorocebus aethiops; Animals; Ligands; Vero Cells; HEK293 Cells; Peptides / chemistry; Peptides / isolation & purification; Peptides / metabolism; Adenoviridae / chemistry; Adenoviridae / isolation & purification; Capsid Proteins / isolation & purification; Capsid Proteins / metabolism; Capsid Proteins / chemistry
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 14, 2024

2024 journal article

Peptide ligands for the universal purification of exosomes by affinity chromatography

Biotechnology and Bioengineering, 121(11), 3484–3501.

By: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , W. Tian * ...

Contributors: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , . al.

author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
MeSH headings : Exosomes / chemistry; Exosomes / metabolism; Chromatography, Affinity / methods; Ligands; Humans; Peptides / chemistry; Peptides / metabolism; Peptides / isolation & purification
topics (OpenAlex): Extracellular vesicles in disease; RNA Interference and Gene Delivery; Inhalation and Respiratory Drug Delivery; Lipid Membrane Structure and Behavior
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 14, 2024

2023 journal article

Molecular Engineering of Cyclic Azobenzene‐Peptide Hybrid Ligands for the Purification of Human Blood Factor VIII via Photo‐Affinity Chromatography

Advanced Functional Materials, 33(14), 2213881.

By: R. Prodromou n, B. Moore n, W. Chu n, H. Deal n, A. San Miguel n , A. Brown n , M. Daniele n , V. Pozdin*, S. Menegatti n 

Contributors: R. Prodromou n, B. Moore n, W. Chu n, H. Deal n, A. Miguel n , A. Brown n , M. Daniele n , V. Pozdin*, S. Menegatti n 

author keywords: affinity ligands; azobenzene-peptide hybrids; biomolecular recognition; factor VIII; photo-affinity chromatography
topics (OpenAlex): Hemophilia Treatment and Research; Platelet Disorders and Treatments; Cancer-related gene regulation
TL;DR: The cyclic azobenzene‐peptide ligands introduced in this report demonstrate a novel route integrating gentle operational conditions in a rapid and efficient bioprocess for the purification of life‐saving biotherapeutics. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 20, 2023

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2023 journal article

Peptide ligands targeting the vesicular stomatitis virus G (VSV‐G) protein for the affinity purification of lentivirus particles

Biotechnology and Bioengineering, 121(2), 618–639.

By: E. Barbieri n, G. Mollica n, B. Moore n, S. Sripada n, S. Shastry n, R. Kilgore n, C. Loudermilk n, Z. Whitacre n ...

author keywords: affinity chromatography; cell therapy; lentivirus; peptide ligands; vesicular stomatitis virus G protein
MeSH headings : Animals; Humans; Lentivirus / genetics; Lentivirus / metabolism; Vesicular Stomatitis; HEK293 Cells; Peptides / metabolism; Vesiculovirus / genetics; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Virology and Viral Diseases; Bacteriophages and microbial interactions
TL;DR: This study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV‐G) to serve as affinity ligands for the chromatographic purification of LV particles. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 29, 2024

2022 article

Development of peptide ligands for the purification of α-1 antitrypsin from cell culture fluids

Chu, W., Prodromou, R., Moore, B., Elhanafi, D., Kilgore, R., Shastry, S., & Menegatti, S. (2022, July 22). Journal of Chromatography A, Vol. 1679.

By: W. Chu n, R. Prodromou n, B. Moore n, D. Elhanafi n, R. Kilgore n, S. Shastry n, S. Menegatti n 

author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
topics (OpenAlex): Protein Hydrolysis and Bioactive Peptides; Silk-based biomaterials and applications; Chemical Synthesis and Analysis
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Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022

2020 article

Peptides and pseudopeptide ligands: a powerful toolbox for the affinity purification of current and next-generation biotherapeutics

Chu, W., Prodromou, R., Day, K. N., Schneible, J. D., Bacon, K. B., Bowen, J. D., … Menegatti, S. (2020, October 23). Journal of Chromatography A, Vol. 1635.

By: W. Chu n, R. Prodromou n, K. Day n, J. Schneible n, K. Bacon n, J. Bowen n, R. Kilgore n, C. Catella n ...

author keywords: Affinity ligands; peptides; peptoids; pseudopeptides; biotherapeutics
MeSH headings : Antibodies / isolation & purification; Biological Products / isolation & purification; Chemistry, Pharmaceutical / methods; Chromatography, Affinity; Family Characteristics; Humans; Ligands; Peptides / isolation & purification; Peptoids / chemistry; Proteins / isolation & purification
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Chemical Synthesis and Analysis; Click Chemistry and Applications
TL;DR: This work presents a comprehensive and systematic review of the literature on peptide-based ligands and their use in the affinity purification of established and upcoming biological drugs. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: January 25, 2021

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