Balaji Rao

Karakis, V., Jabeen, M., Britt, J. W., Cordiner, A., Mischler, A., Li, F., … Rao, B. M. (2023). Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions. JOURNAL OF BIOLOGICAL CHEMISTRY, 299(5). https://doi.org/10.1016/j.jbc.2023.104650

McArthur, N., Cruz-Teran, C., Thatavarty, A., Reeves, G. T., & Rao, B. M. (2022, July 1). Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase. ACS OMEGA. https://doi.org/10.1021/acsomega.2c02319

McArthur, N., Cruz-Teran, C., Thatavarty, A., Reeves, G. T., & Rao, B. M. (2022). Experimental and Analytical Framework for "Mix-and-Read" Assays Based on Split Luciferase. ACS OMEGA, 7(28), 24551–24560. https://doi.org/10.1021/acsomega.2c0231924551ACS

Meanor, J. N., Keung, A. J., & Rao, B. M. (2022). Modified Histone Peptides Linked to Magnetic Beads Reduce Binding Specificity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(3). https://doi.org/10.3390/ijms23031691

Waldman, A. C., Rao, B. M., & Keung, A. J. (2021). Mapping the residue specificities of epigenome enzymes by yeast surface display. CELL CHEMICAL BIOLOGY, 28(12), 1772-+. https://doi.org/10.1016/j.chembiol.2021.05.022

Bacon, K., Blain, A., Bowen, J., Burroughs, M., McArthur, N., Menegatti, S., & Rao, B. M. (2021). Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions. ACS SYNTHETIC BIOLOGY, 10(3), 505–514. https://doi.org/10.1021/acssynbio.0c00472

Bowen, J., Schneible, J., Bacon, K., Labar, C., Menegatti, S., & Rao, B. M. (2021). Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(4). https://doi.org/10.3390/ijms22041634

Mischler, A., Karakis, V., Mahinthakumar, J., Carberry, C. K., San Miguel, A., Rager, J. E., … Rao, B. M. (2021). Two distinct trophectoderm lineage stem cells from human pluripotent stem cells. JOURNAL OF BIOLOGICAL CHEMISTRY, 296. https://doi.org/10.1016/j.jbc.2021.100386

Mukherjee, A., Singh, R., Udayan, S., Biswas, S., Reddy, P. P., Manmadhan, S., … Gulyani, A. (2020). A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells. ELIFE, 9. https://doi.org/10.7554/eLife.50571

Bowen, J., Schloop, A. E., Reeves, G. T., Menegatti, S., & Rao, B. M. (2020). Discovery of Membrane-Permeating Cyclic Peptides via mRNA Display. BIOCONJUGATE CHEMISTRY, 31(10), 2325–2338. https://doi.org/10.1021/acs.bioconjchem.0c00413

Kesarwani, S., Lama, P., Chandra, A., Reddy, P. P., Jijumon, A. S., Bodakuntla, S., … Sirajuddin, M. (2020). Genetically encoded live-cell sensor for tyrosinated microtubules. JOURNAL OF CELL BIOLOGY, 219(10). https://doi.org/10.1083/jcb.201912107

Bacon, K., Blain, A., Burroughs, M., McArthrur, N., Rao, B. M., & Menegatti, S. (2020). Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display. ACS COMBINATORIAL SCIENCE, 22(10), 519–532. https://doi.org/10.1021/acscombsci.0c00076

Bacon, K., Lavoie, A., Rao, B. M., Daniele, M., & Menegatti, S. (2020). [Review of Past, Present, and Future of Affinity-based Cell Separation Technologies]. ACTA BIOMATERIALIA, 112, 29–51. https://doi.org/10.1016/j.actbio.2020.05.004

Cruz-Teran, C. A., Bacon, K., & Rao, B. M. (2020). Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping. GENOTYPE PHENOTYPE COUPLING: METHODS AND PROTOCOLS, Vol. 2070, pp. 321–334. https://doi.org/10.1007/978-1-4939-9853-1_18

Bacon, K., Bowen, J., Reese, H., Rao, B. M., & Menegatti, S. (2020). Use of Target-Displaying Magnetized Yeast in Screening mRNA-Display Peptide Libraries to Identify Ligands. ACS COMBINATORIAL SCIENCE, 22(12), 738–744. https://doi.org/10.1021/acscombsci.0c00171

Mischler, A., Karakis, V., San Miguel, A., & Rao, B. (2019, August). DERIVATION OF HUMAN TROPHOBLAST STEM CELLS FROM HUMAN PLURIPOTENT STEM CELLS. PLACENTA, Vol. 83, pp. E59–E59. https://doi.org/10.1016/j.placenta.2019.06.193

Tiruthani, K., Mischler, A., Ahmed, S., Mahinthakumar, J., Haugh, J. M., & Rao, B. M. (2019). Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation. SCIENCE SIGNALING, 12(584). https://doi.org/10.1126/scisignal.aap7584

Bacon, K., Burroughs, M., Blain, A., Menegatti, S., & Rao, B. M. (2019). Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders. ACS COMBINATORIAL SCIENCE, 21(12), 817–832. https://doi.org/10.1021/acscombsci.9b00147

Cruz-Teran, C. A., Bacon, K., McArthur, N., & Rao, B. M. (2018). An Engineered Sso7d Variant Enables Efficient Magnetization of Yeast Cells. ACS COMBINATORIAL SCIENCE, 20(10), 579–584. https://doi.org/10.1021/acscombsci.8b00084

Patil, R., Miles, J., Ko, Y., Datta, P., Rao, B. M., Kiserow, D., & Genzer, J. (2018). Kinetic Study of Degrafting Poly(methyl methacrylate) Brushes from Flat Substrates by Tetrabutylammonium Fluoride. MACROMOLECULES, 51(24), 10237–10245. https://doi.org/10.1021/acs.macromol.8b01832

Mischler, A., & Rao, B. (2017). A defined and serum-free culture system for trophoblast differentiation of human embryonic stem cells. Placenta, 57, 237–238. https://doi.org/10.1016/J.PLACENTA.2017.07.059

Miles, J., Schlenker, S., Ko, Y., Patil, R., Rao, B. M., & Genzer, J. (2017). Design and Fabrication of Wettability Gradients with Tunable Profiles through Degrafting Organosilane Layers from Silica Surfaces by Tetrabutylammonium Fluoride. LANGMUIR, 33(51), 14556–14564. https://doi.org/10.1021/acs.langmuir.7b02961

Albrecht, C., Baker, J. C., Blundell, C., Chavez, S. L., Carbone, L., Chamley, L., … O'Tierney-Ginn, P. F. (2017). IFPA meeting 2016 workshop report I: Genomic communication, bioinformatics, trophoblast biology and transport systems. Placenta, 60, S5–S9. https://doi.org/10.1016/J.PLACENTA.2017.01.103

Gocha, T., Rao, B. M., & DasGupta, R. (2017). Identification and characterization of a novel Sso7d scaffold-based binder against Notch1. SCIENTIFIC REPORTS, 7. https://doi.org/10.1038/s41598-017-12246-1

Cruz-Teran, C. A., Tiruthani, K., Mischler, A., & Rao, B. M. (2017). Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae. ACS SYNTHETIC BIOLOGY, 6(11), 2096–2107. https://doi.org/10.1021/acssynbio.7b00144

Sarkar, P., Mischler, A., & Rao, B. (2016). BMP signaling plays a biphasic role during trophoblast differentiation of human embryonic stem cells. Placenta, 45, 99–100. https://doi.org/10.1016/J.PLACENTA.2016.06.135

Carlin, K. B., Cruz-Teran, C. A., Kumar, J. P., Gomes, C., & Rao, B. M. (2016). Combinatorial Pairwise Assembly Efficiently Generates High Affinity Binders and Enables a "Mix-and-Read" Detection Scheme. ACS SYNTHETIC BIOLOGY, 5(12), 1348–1354. https://doi.org/10.1021/acssynbio.6b00034

Sarkar, P., Mischler, A., Randall, S. M., Collier, T. S., Dorman, K. F., Boggess, K. A., … Rao, B. M. (2016). Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts. JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444. https://doi.org/10.1021/acs.jproteome.5b01118

Cruz-Teran, C. A., Carlin, K. B., Efimenko, K., Genzer, J., & Rao, B. M. (2016). Targeted Mutagenesis and Combinatorial Library Screening Enables Control of Protein Orientation on Surfaces and Increased Activity of Adsorbed Proteins. LANGMUIR, 32(34), 8660–8667. https://doi.org/10.1021/acs.langmuir.6b01446

Sarkar, P., Randall, S. M., Collier, T. S., Nero, A., Russell, T. A., Muddiman, D. C., & Rao, B. M. (2015). Activin/Nodal Signaling Switches the Terminal Fate of Human Embryonic Stem Cell-derived Trophoblasts. JOURNAL OF BIOLOGICAL CHEMISTRY, 290(14), 8834–8848. https://doi.org/10.1074/jbc.m114.620641

Hussain, M., Lockney, D., Wang, R., Gera, N., & Rao, B. M. (2013). Avidity-mediated virus separation using a hyperthermophilic affinity ligand. BIOTECHNOLOGY PROGRESS, 29(1), 237–246. https://doi.org/10.1002/btpr.1655

Goli, K. K., Gera, N., Liu, X., Rao, B. M., Rojas, O. J., & Genzer, J. (2013). Generation and Properties of Antibacterial Coatings Based on Electrostatic Attachment of Silver Nanoparticles to Protein-Coated Polypropylene Fibers. ACS APPLIED MATERIALS & INTERFACES, 5(11), 5298–5306. https://doi.org/10.1021/am4011644

Canbolat, M. F., Gera, N., Tang, C., Monian, B., Rao, B. M., Pourdeyhimi, B., & Khan, S. A. (2013). Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast. ACS APPLIED MATERIALS & INTERFACES, 5(19), 9349–9354. https://doi.org/10.1021/am4022768

Gera, N., Hussain, M., & Rao, B. M. (2013). [Review of Protein selection using yeast surface display]. METHODS, 60(1), 15–26. https://doi.org/10.1016/j.ymeth.2012.03.014

Hussain, M., Gera, N., Hill, A. B., & Rao, B. M. (2013). Scaffold Diversification Enhances Effectiveness of a Super library of Hyperthermophilic Proteins. ACS SYNTHETIC BIOLOGY, 2(1), 6–13. https://doi.org/10.1021/sb300029m

Tiruthani, K., Sarkar, P., & Rao, B. (2013). [Review of Trophoblast differentiation of human embryonic stem cells]. BIOTECHNOLOGY JOURNAL, 8(4), 421-+. https://doi.org/10.1002/biot.201200203

Menegatti, S., Hussain, M., Naik, A. D., Carbonell, R. G., & Rao, B. M. (2013). mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands. Biotechnology and Bioengineering, 110(3), 857–870. https://doi.org/10.1002/bit.24760

Gera, N., Hill, A. B., White, D. P., Carbonell, R. G., & Rao, B. M. (2012). Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold. PLOS ONE, 7(11). https://doi.org/10.1371/journal.pone.0048928

Sarkar, P., Randall, S. M., Muddiman, D. C., & Rao, B. M. (2012). Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells. MOLECULAR & CELLULAR PROTEOMICS, 11(12), 1829–1839. https://doi.org/10.1074/mcp.m112.020503

Sarkar, P., Collier, T. S., Randall, S. M., Muddiman, D. C., & Rao, B. M. (2012). The subcellular proteome of undifferentiated human embryonic stem cells. PROTEOMICS, 12(3), 421–430. https://doi.org/10.1002/pmic.201100507

Collier, T. S., Randall, S. M., Sarkar, P., Rao, B. M., Dean, R. A., & Muddiman, D. C. (2011). Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532. https://doi.org/10.1002/rcm.5151

Gera, N., Hussain, M., Wright, R. C., & Rao, B. M. (2011). Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold. JOURNAL OF MOLECULAR BIOLOGY, 409(4), 601–616. https://doi.org/10.1016/j.jmb.2011.04.020

Sarkar, P., & Rao, B. M. (2011). Role of Signaling Pathways and Epigenetic Factors in Lineage Determination During Human Embryonic Stem Cell Differentiation. Embryonic Stem Cells - Differentiation and Pluripotent Alternatives, 1, 1.

Collier, T. S., Sarkar, P., Franck, W. L., Rao, B. M., Dean, R. A., & Muddiman, D. C. (2010). Direct Comparison of Stable Isotope Labeling by Amino Acids in Cell Culture and Spectral Counting for Quantitative Proteomics. ANALYTICAL CHEMISTRY, 82(20), 8696–8702. https://doi.org/10.1021/ac101978b

Collier, T. S., Sarkar, P., Rao, B., & Muddiman, D. (2010). Quantitative top-down proteomics of SILAC labeled human embryonic stem cells. Journal of the American Society for Mass Spectrometry, 21(6), 879–889. https://doi.org/10.1016/j.jasms.2010.01.031

Sarkar, P., & Rao, B. M. (2009). Molecular aspects of cardiac differentiation in embryonic stem cells. Critical Reviews in Biomedical Engineering, 37(4-5), 283–320. https://doi.org/10.1615/critrevbiomedeng.v37.i4-5.10

Varelas, X., Sakuma, R., Samavarchi-Tehrani, P., Peerani, R., Rao, B. M., Dembowy, J., … Wrana, J. L. (2008). TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. NATURE CELL BIOLOGY, 10(7), 837–848. https://doi.org/10.1038/ncb1748

Peerani, R., Rao, B. M., Bauwens, C., Yin, T., Wood, G. A., Nagy, A., … Zandstra, P. W. (2007). Niche-mediated control of human embryonic stem cell self-renewal and differentiation. EMBO JOURNAL, 26(22), 4744–4755. https://doi.org/10.1038/sj.emboj.7601896

Cochran, J. R., Kim, Y.-S., Lippow, S. M., Rao, B., & Wittrup, K. D. (2006). Improved mutants from directed evolution are biased to orthologous substitutions. PROTEIN ENGINEERING DESIGN & SELECTION, 19(6), 245–253. https://doi.org/10.1093/protein/gzl006

Rao, B. M., & Zandstra, P. W. (2005). [Review of Culture development for human embryonic stem cell propagation: molecular aspects and challenges]. CURRENT OPINION IN BIOTECHNOLOGY, 16(5), 568–576. https://doi.org/10.1016/j.copbio.2005.08.001

Rao, B. M., Lauffenburger, D. A., & Wittrup, K. D. (2005). Engineering protein therapeutics within a systems-level computational context. Nature Biotechnology, 23(2), 191–194.

Rao, B. M., Driver, I., Lauffenburger, D. A., & Wittrup, K. D. (2005). High-Affinity CD25-Binding IL-2 Mutants Potently Stimulate Persistent T Cell Growth†. Biochemistry, 44(31), 10696–10701. https://doi.org/10.1021/bi050436x

Rao, B. M., Lauffenburger, D. A., & Wittrup, K. D. (2005). Integrating cell-level kinetic modeling into the design of engineered protein therapeutics. NATURE BIOTECHNOLOGY, 23(2), 191–194. https://doi.org/10.1038/nbt1064

Rao, B. M., Driver, I., Lauffenburger, D. A., & Wittrup, K. D. (2004). Interleukin 2 (IL-2) variants engineered for increased IL-2 receptor alpha-subunit affinity exhibit increased potency arising from a cell surface ligand reservoir effect. Molecular Pharmacology, 66(4), 864–869.

Rao, B. M., Girvin, A. T., Ciardelli, T., Lauffenburger, D. A., & Wittrup, K. D. (2003). Interleukin-2 mutants with enhanced alpha-receptor subunit binding affinity. PROTEIN ENGINEERING, 16(12), 1081–1087. https://doi.org/10.1093/protein/gzg111