@misc{yada_musana_chelangat_osaru_anyanga_katungisa_oloka_ssali_mugisa_2023, title={Breeding Cultivars for Resistance to the African Sweetpotato Weevils, Cylas puncticollis and Cylas brunneus, in Uganda: A Review of the Current Progress}, volume={14}, ISSN={["2075-4450"]}, url={https://www.mdpi.com/2075-4450/14/11/837}, DOI={10.3390/insects14110837}, abstractNote={In sub-Saharan Africa, sweetpotato weevils are the major pests of cultivated sweetpotato, causing estimated losses of between 60% and 100%, primarily during dry spells. The predominantly cryptic feeding behavior of Cylas spp. within their roots makes their control difficult, thus, host plant resistance is one of the most promising lines of protection against these pests. However, limited progress has been made in cultivar breeding for weevil resistance, partly due to the complex hexaploid genome of sweetpotato, which complicates conventional breeding, in addition to the limited number of genotypes with significant levels of resistance for use as sources of resistance. Pollen sterility, cross incompatibility, and poor seed set and germination in sweetpotato are also common challenges in improving weevil resistance. The accurate phenotyping of sweetpotato weevil resistance to enhance the efficiency of selection has been equally difficult. Genomics-assisted breeding, though in its infancy stages in sweetpotato, has a potential application in overcoming some of these barriers. However, it will require the development of more genomic infrastructure, particularly single-nucleotide polymorphism markers (SNPs) and robust next-generation sequencing platforms, together with relevant statistical procedures for analyses. With the recent advances in genomics, we anticipate that genomic breeding for sweetpotato weevil resistance will be expedited in the coming years. This review sheds light on Uganda’s efforts, to date, to breed against the Cylas puncticollis (Boheman) and Cylas brunneus (Fabricius) species of African sweetpotato weevil.}, number={11}, journal={INSECTS}, author={Yada, Benard and Musana, Paul and Chelangat, Doreen M. and Osaru, Florence and Anyanga, Milton O. and Katungisa, Arnold and Oloka, Bonny M. and Ssali, Reuben T. and Mugisa, Immaculate}, year={2023}, month={Nov} } @article{ayesiga_rubaihayo_oloka_dramadri_sserumaga_2023, title={Genome-wide association study and pathway analysis to decipher loci associated with Fusarium ear rot resistance in tropical maize germplasm}, volume={11}, ISSN={["1573-5109"]}, DOI={10.1007/s10722-023-01793-4}, abstractNote={Abstract}, journal={GENETIC RESOURCES AND CROP EVOLUTION}, author={Ayesiga, Stella Bigirwa and Rubaihayo, Patrick and Oloka, Bonny Michael and Dramadri, Isaac Ozinga and Sserumaga, Julius Pyton}, year={2023}, month={Nov} } @book{namanda_oloka_rajendran_mcewan_namazzi_ogero_mwanga_low_adikini_kyalo_et al._2023, title={Guidelines for construction and management of mini screenhouse for sweetpotato seed production}, ISBN={9789290606536}, author={Namanda, S. and Oloka, B.M. and Rajendran, S. and McEwan, M. and Namazzi, S. and Ogero, K. and Mwanga, R.O.M. and Low, Jan W. and Adikini, S. and Kyalo, G. and et al.}, year={2023} } @article{mugisa_karungi_musana_odama_anyanga_edema_gibson_ssali_campos_oloka_et al._2023, title={Heterotic gains, transgressive segregation and fitness cost of sweetpotato weevil resistance expression in a partial diallel cross of sweetpotato}, volume={219}, ISSN={["1573-5060"]}, DOI={10.1007/s10681-023-03225-x}, abstractNote={Abstract}, number={10}, journal={EUPHYTICA}, author={Mugisa, Immaculate and Karungi, Jeninah and Musana, Paul and Odama, Roy and Anyanga, Milton O. and Edema, Richard and Gibson, Paul and Ssali, Reuben T. and Campos, Hugo and Oloka, Bonny M. and et al.}, year={2023}, month={Oct} } @article{osaru_karungi_odama_chelangat_musana_otema_oloka_gibson_edema_ssali_et al._2023, title={Identification of the key morphological sweetpotato weevil resistance predictors in Ugandan sweetpotato genotypes using correlation and path‐coefficient analysis}, volume={63}, ISSN={0011-183X 1435-0653}, url={http://dx.doi.org/10.1002/csc2.20915}, DOI={10.1002/csc2.20915}, abstractNote={Abstract}, number={3}, journal={Crop Science}, publisher={Wiley}, author={Osaru, Florence and Karungi, Jeninah and Odama, Roy and Chelangat, Doreen Murenju and Musana, Paul and Otema, Milton Anyanga and Oloka, Bonny and Gibson, Paul and Edema, Richard and Ssali, Reuben Tendo and et al.}, year={2023}, month={Mar}, pages={1126–1138} } @article{ojwang_okello_otieno_mutiso_lindqvist-kreuze_coaldrake_mendes_andrade_sharma_gruneberg_et al._2023, title={Targeting market segment needs with public-good crop breeding investments: A case study with potato and sweetpotato focused on poverty alleviation, nutrition and gender}, volume={14}, ISSN={1664-462X}, url={http://dx.doi.org/10.3389/fpls.2023.1105079}, DOI={10.3389/fpls.2023.1105079}, abstractNote={Crop breeding programs have often focused on the release of new varieties that target yield improvement to achieve food security and reduce poverty. While continued investments in this objective are justified, there is a need for breeding programs to be increasingly more demand-driven and responsive to the changing customer preferences and population dynamics. This paper analyses the responsiveness of global potato and sweetpotato breeding programs pursued by the International Potato Center (CIP) and its partners to three major development indicators: poverty, malnutrition and gender. The study followed a seed product market segmentation blueprint developed by the Excellence in Breeding platform (EiB) to identify, describe, and estimate the sizes of the market segments at subregional levels. We then estimated the potential poverty and nutrition impacts of investments in the respective market segments. Further, we employed the G+ tools involving multidisciplinary workshops to evaluate the gender-responsiveness of the breeding programs. Our analysis reveals that future investments in breeding programs will achieve greater impacts by developing varieties for market segments and pipelines that have more poor rural people, high stunting rates among children, anemia prevalence among women of reproductive age, and where there is high vitamin A deficiency. In addition, breeding strategies that reduce gender inequality and enhance appropriate change of gender roles (hence gender transformative) are also required.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Ojwang, Sylvester Okoth and Okello, Julius Juma and Otieno, David Jakinda and Mutiso, Janet Mwende and Lindqvist-Kreuze, Hannele and Coaldrake, Peter and Mendes, Thiago and Andrade, Maria and Sharma, Neeraj and Gruneberg, Wolfgang and et al.}, year={2023}, month={Mar} } @article{mugisa_karungi_musana_odama_alajo_chelangat_anyanga_oloka_gonçalves dos santos_talwana_et al._2022, title={Combining ability and heritability analysis of sweetpotato weevil resistance, root yield, and dry matter content in sweetpotato}, volume={13}, ISSN={1664-462X}, url={http://dx.doi.org/10.3389/fpls.2022.956936}, DOI={10.3389/fpls.2022.956936}, abstractNote={Efficient breeding and selection of superior genotypes requires a comprehensive understanding of the genetics of traits. This study was aimed at establishing the general combining ability (GCA), specific combining ability (SCA), and heritability of sweetpotato weevil (Cylasspp.) resistance, storage root yield, and dry matter content in a sweetpotato multi-parental breeding population. A population of 1,896 F1clones obtained from an 8 × 8 North Carolina II design cross was evaluated with its parents in the field at two sweetpotato weevil hotspots in Uganda, using an augmented row-column design. Clone roots were further evaluated in three rounds of a no-choice feeding laboratory bioassay. Significant GCA effects for parents and SCA effects for families were observed for most traits and all variance components were highly significant (p ≤ 0.001). Narrow-sense heritability estimates for weevil severity, storage root yield, and dry matter content were 0.35, 0.36, and 0.45, respectively. Parental genotypes with superior GCA for weevil resistance included “Mugande,” NASPOT 5, “Dimbuka-bukulula,” and “Wagabolige.” On the other hand, families that displayed the highest levels of resistance to weevils included “Wagabolige” × NASPOT 10 O, NASPOT 5 × “Dimbuka-bukulula,” “Mugande” × “Dimbuka-bukulula,” and NASPOT 11 × NASPOT 7. The moderate levels of narrow-sense heritability observed for the traits, coupled with the significant GCA and SCA effects, suggest that there is potential for their improvement through conventional breedingviahybridization and progeny selection and advancement. Although selection for weevil resistance may, to some extent, be challenging for breeders, efforts could be boosted through applying genomics-assisted breeding. Superior parents and families identified through this study could be deployed in further research involving the genetic improvement of these traits.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Mugisa, Immaculate and Karungi, Jeninah and Musana, Paul and Odama, Roy and Alajo, Agnes and Chelangat, Doreen M. and Anyanga, Milton O. and Oloka, Bonny M. and Gonçalves dos Santos, Iara and Talwana, Herbert and et al.}, year={2022}, month={Sep} } @article{ayesiga_rubaihayo_oloka_dramadri_edema_sserumaga_2022, title={Genetic Variation Among Tropical Maize Inbred Lines from NARS and CGIAR Breeding Programs}, volume={41}, ISSN={0735-9640 1572-9818}, url={http://dx.doi.org/10.1007/s11105-022-01358-2}, DOI={10.1007/s11105-022-01358-2}, abstractNote={Abstract}, number={2}, journal={Plant Molecular Biology Reporter}, publisher={Springer Science and Business Media LLC}, author={Ayesiga, Stella Bigirwa and Rubaihayo, Patrick and Oloka, Bonny Michael and Dramadri, Isaac Onziga and Edema, Richard and Sserumaga, Julius Pyton}, year={2022}, month={Sep}, pages={209–217} } @article{mwanga_swanckaert_da silva pereira_andrade_makunde_grüneberg_kreuze_david_de boeck_carey_et al._2021, title={Breeding Progress for Vitamin A, Iron and Zinc Biofortification, Drought Tolerance, and Sweetpotato Virus Disease Resistance in Sweetpotato}, volume={5}, ISSN={2571-581X}, url={http://dx.doi.org/10.3389/fsufs.2021.616674}, DOI={10.3389/fsufs.2021.616674}, abstractNote={Sweetpotato is a resilient food crop with great potential to contribute to reduced hunger in the world. Sweetpotato shows significant potential to contribute to reducing the Global Hunger Index, which reflects deficiencies in calories and micronutrients based on the components of hunger, undernourishment, under-five mortality rate, stunting and wasting. Its genetic diversity has been harnessed through breeding to increase vitamin A, iron, and zinc content, virus resistance and climate resilience for the world's food needs. Africa and India are the most food-insecure regions. The main objectives of this research were to: provide information and a knowledge base on sweetpotato breeding in Africa for biofortification of vitamin A, iron, and zinc, drought tolerance and virus resistance; recommend procedures for generating new breeding populations and varieties; and develop new tools, technologies and methods for sweetpotato improvement. The research was implemented between 2009 and 2020 in 14 collaborating African countries using introduced and local genotypes. The redesigned accelerated breeding scheme resulted in increased genetic gains for vitamin A, iron, zinc contents and virus resistance, and the release by sub-Saharan African countries of 158 varieties; 98 of them orange-fleshed; 55 varieties bred by an accelerated breeding scheme; 27 drought-tolerant and two with enhanced iron and zinc content. Our experience has demonstrated that through the use of more optimized, standardized and collaborative breeding procedures by breeding programs across Africa, it is possible to speed official sweetpotato variety release and contribute to reducing the severe micronutrient deficiencies on the continent.}, journal={Frontiers in Sustainable Food Systems}, publisher={Frontiers Media SA}, author={Mwanga, Robert O. M. and Swanckaert, Jolien and da Silva Pereira, Guilherme and Andrade, Maria I. and Makunde, Godwill and Grüneberg, Wolfgang J. and Kreuze, Jan and David, Maria and De Boeck, Bert and Carey, Edward and et al.}, year={2021}, month={Mar} } @article{oloka_da silva pereira_amankwaah_mollinari_pecota_yada_olukolu_zeng_craig yencho_2021, title={Discovery of a major QTL for root-knot nematode (Meloidogyne incognita) resistance in cultivated sweetpotato (Ipomoea batatas)}, volume={134}, ISSN={0040-5752 1432-2242}, url={http://dx.doi.org/10.1007/s00122-021-03797-z}, DOI={10.1007/s00122-021-03797-z}, abstractNote={Abstract}, number={7}, journal={Theoretical and Applied Genetics}, publisher={Springer Science and Business Media LLC}, author={Oloka, Bonny Michael and da Silva Pereira, Guilherme and Amankwaah, Victor A. and Mollinari, Marcelo and Pecota, Kenneth V. and Yada, Benard and Olukolu, Bode A. and Zeng, Zhao-Bang and Craig Yencho, G.}, year={2021}, month={Apr}, pages={1945–1955} } @book{schultheis_oloka_collins_2020, place={Raleigh, NC}, title={Growing Jerusalem Artichokes}, url={https://content.ces.ncsu.edu/growing-jerusalem-artichokes}, institution={NC State Cooperative Extension Publications}, author={Schultheis, Johnathan and Oloka, Bonny Michael and Collins, Maxton}, year={2020} } @inproceedings{yada_oloka_olukolu_anyanga_chelangat_musana_alajo_da silva pereira_mollinari_zeng_et al._2019, title={Identification of Quantitative Trait Loci of Storage Root Micronutrients (Iron and Zinc) in Cultivated Sweetpotato}, booktitle={Plant and Animal Genome Conference XXVII}, author={Yada, B. and Oloka, B.M. and Olukolu, B.A. and Anyanga, M.O. and Chelangat, C. and Musana, P. and Alajo, A. and Da Silva Pereira, G. and Mollinari, M. and Zeng, Z.-B. and et al.}, year={2019}, month={Jan} } @inproceedings{oloka_yada_olukolu_anyanga_chelangat_musana_alajo_da silva pereira_mollinari_zeng_et al._2019, title={Linkage and QTL Analysis for Sweetpotato Virus Disease Resistance in a bi-parental Sweetpotato Population}, booktitle={Plant and Animal Genome Conference XXVII}, author={Oloka, B.M. and Yada, B. and Olukolu, B. and Anyanga, M.O. and Chelangat, D. and Musana, P. and Alajo, A. and Da Silva Pereira, G. and Mollinari, M. and Zeng, Z.-B. and et al.}, year={2019}, month={Jan} } @inproceedings{oloka_yada_olukolu_da silva pereira_anyanga_amankwaah_mollinari_chelangat_musana_alajo_et al._2018, title={Genetic Linkage and QTL Analysis in two bi-parental Sweetpotato Mapping Populations}, booktitle={17th Annual Sweetpotato Speedbreeders Meeting}, author={Oloka, B.M. and Yada, B. and Olukolu, B. and da Silva Pereira, G. and Anyanga, M.O. and Amankwaah, V. and Mollinari, M. and Chelangat, D. and Musana, P. and Alajo, A. and et al.}, year={2018}, month={Jun} } @inproceedings{oloka_olukolu_yada_anyanga_chelangar_musana_alajo_da silva pereira_mollinari_zeng_et al._2018, place={Gainesville, FL, USA}, title={Linkage and QTL Analysis in a Hexaploid Sweetpotato Mapping Population}, booktitle={Plant Science Symposium}, publisher={Plant Science Symposium, University of Florida}, author={Oloka, B.M. and Olukolu, B. and Yada, B. and Anyanga, M.O. and Chelangar, D. and Musana, P. and Alajo, A. and da Silva Pereira, G. and Mollinari, M. and Zeng, Z.-B. and et al.}, year={2018}, month={Jan} } @inproceedings{oloka_olukolu_yada_anyanga_chelangat_musana_alajo_da silva pereira_mollinari_zeng_et al._2018, title={Linkage and QTL Analysis in the Hexaploid New Kawogo X Beauregard Mapping Population}, booktitle={Plant and Animal Genome Conference XXVI}, author={Oloka, B.M. and Olukolu, B. and Yada, B. and Anyanga, M.O. and Chelangat, D. and Musana, P. and Alajo, A. and Da Silva Pereira, G. and Mollinari, M. and Zeng, Z.-B. and et al.}, year={2018}, month={Jan} } @inproceedings{oloka_2017, title={Genetic Mapping of Nematode Resistance in a Bi-parental Sweetpotato Population}, booktitle={Annual meeting: Southern Region American Society for Horticultural Science}, author={Oloka, B.M.}, year={2017}, month={Jan} } @inproceedings{oloka_2017, title={Genetic Mapping of Sweetpotato Virus Disease and Root Knot Nematode Resistance in Sweetpotato}, booktitle={3rd Annual GT4SP meeting}, author={Oloka, B.M.}, year={2017}, month={May} } @inproceedings{oloka_2016, title={Genetic Mapping of Sweetpotato Virus Disease and Root Knot Nematode Resistance in Sweetpotato}, booktitle={NC State Horticultural Science Departmental Seminar}, author={Oloka, B.M.}, year={2016}, month={Dec} } @inproceedings{oloka_2016, title={QTL Mapping of Sweetpotato Weevil and Sweetpotato Virus Disease Resistance in Sweetpotato}, booktitle={2nd Annual GT4SP meeting}, author={Oloka, B.M.}, year={2016}, month={Jan} } @article{nyiramugisha_lamo_oloka_ongom_gibson_edema_2016, title={Response to cold stress at reproductive stage of introduced and adapted rice genotypes in Uganda}, volume={14}, number={2}, journal={RUFORUM Working Document Series}, author={Nyiramugisha, J. and Lamo, J. and Oloka, B.M. and Ongom, P. and Gibson, P. and Edema, R.}, year={2016}, month={Jun}, pages={633–638} } @article{lamo_cho_jane_dartey_james_ekobu_alibu_okanya_oloka_otim_et al._2015, title={Developing Lowland Rice Germplasm with Resistance to Multiple Biotic Stresses through Anther Culture in Uganda}, volume={27}, url={http://dx.doi.org/10.12719/ksia.2015.27.4.415}, DOI={10.12719/ksia.2015.27.4.415}, abstractNote={The lowland rice genotypes grown in Uganda were introduced in the 1970s. These genotypes (now landraces) are threatened by multiple biotic stresses namely; Rice Yellow Mottle Virus (RYMV) disease, Bacterial Leaf Streak(BLS). Bacterial Leaf Blight (BLB), and Rice Blast (BL). There are currently no rice lines with multiple resistance to these stresses although attempts have been made to develop them through hybridization involving cultivated, local and introduced lines and four varieties with tolerance to RYMV have been released. The use of potential resistance donor such as the traditional African cultivated rice, Oryza glaberrima, could be an alternative approach to furnish multiple resistance to the cultivated rice. The rice germplasm developed from a cross of an Oryza glaberrima from Niger Delta and Milyang23, a high-yielding Korean rice variety were evaluated for multiple resistance in Uganda as a Korea-Africa Food & Agriculture Cooperation Initiative (KAFACI)-Alliance for a Green Revolution in Africa (AGRA) joint cooperative project, “Enhancement of High Yielding Rice Germplasm in African Countries through Anther Culture Breeding”. Milyang23 was back crossed 4 times with Oryza. glaberrima and fixed through anther culture in Korea. An evaluation of 50 lines generated showed that up to 98%, 92%, 88% and 88% of the test plants showed resistance to the RYMV, BLS, BLB and BL diseases, respectively. There was no symptoms of the four diseases in 74% of the genotypes tested. The plants that showed symptoms of the three diseases had scores of not more than 3 on a 1 to 9 scale. This preliminary finding demonstrates that these generations of rice lines could help solving the current problem of susceptibility to multiple diseases.}, number={4}, journal={Journal of the Korean Society of International Agricultue}, publisher={Korean Society of International Agriculture}, author={Lamo, Jimmy and Cho, Gyoung-rae and Jane, Ininda and Dartey, Paul Kofi Ayirebi and James, Ekebu and Ekobu, Moses and Alibu, Simon and Okanya, Stephen and Oloka, Bonny and Otim, Michael and et al.}, year={2015}, month={Nov}, pages={415–420} } @article{bonny_jimmy_patrick_paul_juan_2015, title={The use of multiplexed simple sequence repeat (SSR) markers for analysis of genetic diversity in African rice genotypes}, volume={14}, url={http://dx.doi.org/10.5897/ajb2015.14478}, DOI={10.5897/ajb2015.14478}, abstractNote={Rice is an emerging food and cash crop in Eastern Africa. Thousands of germplasm accessions have been introduced from major rice breeding centers, such as the International Rice Research Institute (IRRI), and Africa Rice but the genetic variability among the introduced rice germplasm is unknown. Knowledge on genetic diversity would be useful in designing measures for comprehensive breeding and conservation. To address this knowledge gap, 10 highly polymorphic rice simple sequence repeat (SSR) markers were used to characterize 99 rice genotypes to determine their diversity and place them in their different population groups. The SSR markers were multiplexed in 3 panels to increase their throughput. An average of 15.9 alleles was detected, ranging from 6 alleles detected by marker RM7 to 30 by marker RM333. The UPGMA dendogram based on Nei’s genetic distance cluster analysis, revealed 5 genetic groups among the genotypes tested. Analysis of molecular variance indicated that 97% of the diversity observed was explained by differences in the genotypes themselves, and only 3% was due to the sources from which the genotypes were obtained. This study sets the stage for further diversity analysis of all the available germplasm lines using SSR markers to ensure effective utilization and conservation of the germplasm. Keywords: Genetic diversity, simple sequences repeat (SSR) markers, multiplexing, rice genotypes, structure. Abbreviation: IRRI, International Rice Research Institute; SSR, simple sequence repeat; NaCRRI, national crops resources research institute; RAPD, random amplified polymorphic DNA; AFLP, amplified fragment length polymorphisms; RFLP, restriction fragment length polymorphisms; SNP, single nucleotide polymorphisms; BAC, bacterial artificial chromosome; PAC, P1-derived artificial chromosome; PCR, polymerase chain reaction; Ho, heterozygosity; He, heterozygosity.}, number={18}, journal={African Journal of Biotechnology}, publisher={Academic Journals}, author={Bonny, M Oloka and Jimmy, Lamo and Patrick, Rubaihayo and Paul, Gibson and Juan, Vorster}, year={2015}, month={May}, pages={1533–1542} } @inproceedings{lamo_gyoung-rae_ininda_kofi ayirebi_ekebu_ekobu_alibu_okanya_oloka_otim_et al._2014, title={Developing Lowland Rice Varieties with Resistance to Multiple Biotic Stresses}, booktitle={2nd Biennial NARO Scientific Conference}, author={Lamo, J. and Gyoung-rae, C. and Ininda, J. and Kofi Ayirebi, D.P. and Ekebu, J. and Ekobu, M. and Alibu, S. and Okanya, S. and Oloka, B. and Otim, M. and et al.}, year={2014}, month={Nov} } @inproceedings{oloka_2014, title={Molecular Characterization of Rice Genotypes in Uganda Using Multiplex SSR Method}, booktitle={4th International Rice Congress}, author={Oloka, B.M.}, year={2014}, month={Oct} } @inproceedings{oloka_2014, title={Rice (Oryza sp) Germplasm Diversity and breeding for resistance to rice yellow mottle virus (RYMV) in Uganda}, booktitle={Rice Regional Centre of Excellence 1st Scientific Conference}, author={Oloka, B.M.}, year={2014}, month={Jul} } @article{reaction of selected rice genotypes with monogenic resistance to the isolate of magnaporthe oryzae collected at namulonge, uganda }