Benjamin Zeldes

Works (10)

Updated: August 9th, 2023 15:05

2020 journal article

Metabolically engineeredCaldicellulosiruptor besciias a platform for producing acetone and hydrogen from lignocellulose

BIOTECHNOLOGY AND BIOENGINEERING, 117(12), 3799–3808.

By: C. Straub n, R. Bing n , J. Otten n, L. Keller  n, B. Zeldes n, M. Adams *, R. Kelly n 

author keywords: acetone; Caldicellulosiruptor bescii; extreme thermophiles; metabolic engineering
MeSH headings : Acetone / metabolism; Biomass; Caldicellulosiruptor / genetics; Caldicellulosiruptor / metabolism; Hydrogen / metabolism; Lignin / metabolism; Metabolic Engineering
TL;DR: C. bescii can be engineered with balanced pathways in which renewable carbohydrate sources are converted to useful metabolites, primarily acetone and H2, without net production of its native fermentation products, acetate and lactate. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: September 7, 2020

2019 journal article

Determinants of sulphur chemolithoautotrophy in the extremely thermoacidophilic Sulfolobales

ENVIRONMENTAL MICROBIOLOGY, 21(10), 3696–3710.

By: B. Zeldes n, A. Loder n, J. Counts n, M. Haque n, K. Widney  n, L. Keller  n, S. Albers *, R. Kelly n 

MeSH headings : Autotrophic Processes; Chemoautotrophic Growth; Oxidation-Reduction; Oxidoreductases / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thiosulfates / metabolism
TL;DR: Comparative transcriptomic analyses of Acidianus brierleyi, a chemolithoautotrophic sulfur oxidizer, and S. acidocaldarius DSM639 showed that while both share a strong transcriptional response to elemental sulfur, both failed to up-regulate key 3-HP/4-HB cycle genes used by A. briersleyi to drive chemolithOautotrophy. (via Semantic Scholar)
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15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 12, 2019

2018 journal article

A synthetic enzymatic pathway for extremely thermophilic acetone production based on the unexpectedly thermostable acetoacetate decarboxylase from Clostridium acetobutylicum

BIOTECHNOLOGY AND BIOENGINEERING, 115(12), 2951–2961.

By: B. Zeldes n, C. Straub n, J. Otten n, M. Adams* & R. Kelly n 

author keywords: acetone; biotransformations; extreme thermophiles
MeSH headings : Acetone / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carboxy-Lyases / genetics; Carboxy-Lyases / metabolism; Clostridium acetobutylicum / enzymology; Clostridium acetobutylicum / genetics; Clostridium acetobutylicum / metabolism; Enzyme Stability; Hot Temperature; Metabolic Engineering; Metabolic Networks and Pathways / genetics; Synthetic Biology / methods
TL;DR: A synthetic enzymatic pathway for acetone production that functions up to at least 70°C in vitro, made possible by the unusual thermostability of Adc from the mesophile C. acetobutylicum, paving the way for bio‐reactive distillation of acetone using a metabolically engineered extreme thermophile as a production host. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018

2018 review

Biotechnology of extremely thermophilic archaea

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 42(5), 543–578.

By: C. Straub n, J. Counts n, D. Nguyen*, C. Wu*, B. Zeldes n, J. Crosby n, J. Conway  n, J. Otten n ...

author keywords: extremely thermophilic archaea; biotechnology; biocatalysis; biooxidation
MeSH headings : Archaea / genetics; Archaea / physiology; Biotechnology / trends; Hot Temperature; Industrial Microbiology / trends; Metabolic Engineering / trends
TL;DR: There are barriers and challenges to these organisms reaching their full potential as industrial microorganisms but, if these can be overcome, a new dimension for biotechnology will be forthcoming that strategically exploits biology at high temperatures. (via Semantic Scholar)
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15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 19, 2018

2017 review

Extremely thermophilic energy metabolisms: biotechnological prospects

[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 45, 104–112.

By: C. Straub n, B. Zeldes n, G. Schut *, M. Adams * & R. Kelly n 

MeSH headings : Archaea / classification; Archaea / genetics; Archaea / metabolism; Biotechnology / methods; Carbon Dioxide / metabolism; Chemoautotrophic Growth; Energy Metabolism; Hot Temperature; Lignin / metabolism; Metabolic Engineering
TL;DR: This review considers recent developments in extreme thermophile biology as they relate to new horizons for energy biotechnology. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 review

Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms

[Review of ]. WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE, 9(3).

By: J. Counts n, B. Zeldes n, L. Lee n, C. Straub n, M. Adams * & R. Kelly n 

MeSH headings : Biocatalysis; Carbohydrate Metabolism; Carbon Dioxide / metabolism; Glycolysis; Metabolic Engineering; Metals / chemistry; Metals / metabolism; Sulfolobales / metabolism; Sulfur / metabolism; Thermoanaerobacter / metabolism; Thermococcales / metabolism; Thermus / metabolism
TL;DR: The bacterial genera Caldicellulosiruptor, Thermotoga and Thermus, and the archaea belonging to the orders Thermococcales and Sulfolobales, are among the most studied extreme thermophiles to date. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus

Biotechnology and Bioengineering, 113(12), 2652–2660.

By: H. Lian, B. Zeldes, G. Lipscomb, A. Hawkins, Y. Han, A. Loder, D. Nishiyama, M. Adams, R. Kelly

Source: NC State University Libraries
Added: August 6, 2018

2015 journal article

Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(20), 7187–7200.

By: A. Loder n, B. Zeldes n, G. Garrison n, G. Lipscomb*, M. Adams * & R. Kelly n 

MeSH headings : 1-Butanol / metabolism; Acetyl-CoA C-Acyltransferase / metabolism; Acyl Coenzyme A / metabolism; Alcohol Dehydrogenase / metabolism; Alcohol Oxidoreductases / metabolism; Aldehyde Oxidoreductases / metabolism; Biocatalysis; Chromatography, Gas; Clostridium acetobutylicum / metabolism; Clostridium thermocellum / metabolism; Thermoanaerobacter / metabolism
TL;DR: More-selective thermophilic versions of Bad, Bdh, and AdhE are needed to fully exploit biocatalytic n-butanol production at elevated temperatures. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2-based 3-hydroxypropionate production

BIOTECHNOLOGY AND BIOENGINEERING, 112(8), 1533–1543.

By: A. Hawkins n, H. Lian n, B. Zeldes n, A. Loder n, G. Lipscomb*, G. Schut *, M. Keller *, M. Adams *, R. Kelly n 

author keywords: 3-hydroxypropionate; CO2 fixation; Metallosphaera sedula; Pyrococcus furiosus
MeSH headings : Bioreactors / microbiology; Carbon Dioxide / metabolism; Gene Expression Profiling; Hot Temperature; Lactic Acid / analogs & derivatives; Lactic Acid / metabolism; Maltose / metabolism; Metabolic Engineering / methods; Metabolic Networks and Pathways / genetics; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Pyrococcus furiosus / radiation effects; Sulfolobaceae / genetics
TL;DR: The results here support the prospects of using thermally diverse sources of pathways and enzymes in metabolically engineered strains designed for product formation at sub‐optimal growth temperatures. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 review

Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals

[Review of ]. FRONTIERS IN MICROBIOLOGY, 6.

By: B. Zeldes n, M. Keller *, A. Loder n, C. Straub n, M. Adams * & R. Kelly n 

author keywords: extreme thermophiles; metabolic engineering; bio-based chemicals; genetics; biotechnology
TL;DR: The issues and opportunities for extremely thermophilic metabolic engineering platforms are considered with an eye toward potential technological advantages for high temperature industrial biotechnology. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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