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Works (15)
Updated: April 4th, 2024 20:27
2009 journal article
Analysis of potato virus X replicase and TGBp3 subcellular locations
VIROLOGY, 393(2), 272–285.
author keywords: Potexvirus; Potato virus X; Triple gene block; Replicase
MeSH headings : Cell Membrane / virology; Cerulenin / pharmacology; Endoplasmic Reticulum / metabolism; Endoplasmic Reticulum / virology; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Microscopy, Confocal; Microscopy, Electron, Transmission; Plant Leaves / ultrastructure; Plant Leaves / virology; Potexvirus / genetics; Potexvirus / metabolism; Protoplasts / virology; RNA-Dependent RNA Polymerase / genetics; RNA-Dependent RNA Polymerase / metabolism; Tobacco / virology; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
The combined data indicate that PVX replication relies on ER-derived membrane recruitment and membrane proliferation, and there is no evidence to indicate that either PVX protein moves into the Golgi apparatus.
(via Semantic Scholar)
open access via doi.org (publisher)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
Mutations that alter a repeated ACCA element located at the 5 ' end of the Potato virus X genome affect RNA accumulation
VIROLOGY, 378(1), 133–141.
author keywords: PVX; ACCA elements; RNA interaction; replication
MeSH headings : 5' Untranslated Regions / chemistry; 5' Untranslated Regions / genetics; Base Sequence; Gene Deletion; Genome, Viral / genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Plant Leaves / virology; Potexvirus / genetics; Potexvirus / pathogenicity; RNA, Viral / metabolism; Repetitive Sequences, Nucleic Acid / genetics; Sequence Alignment; Tandem Repeat Sequences; Tobacco / virology; Virus Replication
TL;DR:
Detailed characterization of the first ACCA element indicated that duplication of ACCA at nt 10-13 (nt 10-17, ACCAACCA) caused severe symptom development as compared to that of wild type, while deletion of the single element or tripling of this element caused reduced symptom development.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2008 journal article
RNA-RNA interactions between RNA elements at the 5 ' end and at the upstream of sgRNA of RNA genome are required for Potato virus X RNA replication
PLANT PATHOLOGY JOURNAL, 24(3), 289–295.
author keywords: plus-strand RNA accumulation; PVX; replication; RNA-RNA base-pairing
TL;DR:
Results indicate that the RNA element located at the 5` end of PVX genome involved in RNA-RNA interactions and play a key role in high-level accumulation of plus-strand RNA in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Long-distance RNA-RNA interactions between terminal elements and the same subset of internal elements on the potato virus X genome mediate minus- and plus-strand RNA synthesis
RNA, 13(2), 267–280.
author keywords: RNA; replication; PVX; virus; interactions
MeSH headings : Base Sequence; Conserved Sequence; Genome, Viral; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Potexvirus / chemistry; Potexvirus / genetics; Potexvirus / physiology; RNA, Viral / chemistry; RNA, Viral / genetics; RNA, Viral / metabolism; Regulatory Sequences, Ribonucleic Acid; Sequence Analysis, RNA; Virus Replication
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2005 journal article
cis-Acting sequences required for coat protein binding and in vitro assembly of Potato virus X
VIROLOGY, 334(1), 83–97.
author keywords: PVX; assembly; OAS; SELEX; stem-loop structure
MeSH headings : 5' Untranslated Regions; Base Sequence; Binding Sites / genetics; Capsid Proteins / genetics; Capsid Proteins / isolation & purification; Capsid Proteins / metabolism; DNA, Viral / genetics; Escherichia coli / genetics; Genetic Complementation Test; Microscopy, Electron; Molecular Sequence Data; Nucleic Acid Conformation; Potexvirus / genetics; Potexvirus / physiology; Potexvirus / ultrastructure; Protein Binding; RNA, Viral / chemistry; RNA, Viral / genetics; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Virus Assembly / genetics
TL;DR:
Results suggest that RNA secondary structural elements within SL1 and/or sequences therein are crucial for formation of VLPs and are required for the specific recognition by the CP subunit.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2003 journal article
Cis-acting regulatory elements in the potato virus X 3 ' non-translated region differentially affect minus-strand and plus-strand RNA accumulation
JOURNAL OF MOLECULAR BIOLOGY, 326(3), 701–720.
author keywords: potato virus X; RNA replication; RNA structure; RNA virus; stem-loop
MeSH headings : 3' Untranslated Regions; Base Sequence; Molecular Sequence Data; Nucleic Acid Conformation; Potexvirus / genetics; RNA, Viral / chemistry; RNA, Viral / metabolism; Regulatory Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid
TL;DR:
PVX 3′ NTR contains multiple, overlapping elements that influence accumulation of both minus and plus-strand RNA, and potential polyadenylation signals (near upstream elements; NUEs) in the 3″ NTR of PVX RNA were more important for plus-Strand RNA accumulation than for minus-strander RNA accumulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2003 review
Potato virus X: a model system for virus replication, movement and gene expression
[Review of ]. MOLECULAR PLANT PATHOLOGY, 4(2), 125–131.
TL;DR:
The PVX system will continue to serve as a model for analyses of processes such as virus movement, RNA replication, and gene silencing, and as a tool for protein expression.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Cellular protein binds to sequences near the 5 ' terminus of potato virus X RNA that are important for virus replication
VIROLOGY, 301(2), 305–312.
MeSH headings : 5' Untranslated Regions / chemistry; 5' Untranslated Regions / metabolism; 5' Untranslated Regions / physiology; Base Sequence; Binding Sites; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Plant Proteins / metabolism; Potexvirus / genetics; Potexvirus / physiology; Protein Binding; Protoplasts / metabolism; RNA, Viral / chemistry; RNA, Viral / metabolism; RNA, Viral / physiology; RNA-Binding Proteins / metabolism; Tobacco; Virus Replication
TL;DR:
Electrophoretic mobility shift assays with protoplast protein extracts and RNA sequences from the PVX 5'-NTR show that the 5' region of PVX positive-strand RNA formed complexes with cellular proteins, and suggest that the p54 may function in PVX RNA replication by binding to the5' terminus of the viral genomic RNA.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Role of the 3 ' tRNA-like structure in tobacco mosaic virus minus-strand RNA synthesis by the viral RNA-dependent RNA polymerase in vitro
JOURNAL OF VIROLOGY, 74(24), 11671–11680.
MeSH headings : 3' Untranslated Regions / genetics; Base Sequence; DNA-Directed RNA Polymerases / physiology; Molecular Sequence Data; Mutation; Plants, Toxic; RNA, Transfer / genetics; RNA, Viral / physiology; Templates, Genetic; Tobacco / virology; Tobacco Mosaic Virus / physiology; Virus Replication
TL;DR:
Several double-helical regions, but not their sequences, which are essential for forming pseudoknot and/or stem-loop structures in domains D1, D2, and D3 and the central core, C, were shown to be required for high template efficiency.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Soluble, template-dependent extracts from Nicotiana benthamiana plants infected with potato virus X transcribe both plus- and minus-strand RNA templates
VIROLOGY, 275(2), 444–451.
MeSH headings : Electrophoresis, Agar Gel; Plant Extracts / metabolism; Plants, Toxic; Potexvirus / metabolism; Potexvirus / physiology; RNA, Viral / analysis; RNA, Viral / biosynthesis; RNA-Dependent RNA Polymerase / metabolism; Tobacco / virology; Transcription, Genetic; Virus Replication
TL;DR:
A method to convert membrane-bound replication complexes isolated from Nicotiana benthamiana plants infected with potato virus X to a soluble, template-dependent system for analysis of RNA synthesis has been developed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
Long-distance RNA-RNA interactions and conserved sequence elements affect potato virus X plus-strand RNA accumulation
RNA, 5(5), 636–645.
author keywords: cis-acting regulatory elements; plant RNA virus; RNA replication; subgenomic RNA synthesis
MeSH headings : 5' Untranslated Regions / genetics; Macromolecular Substances; Mutagenesis, Site-Directed; Plants, Toxic; Potexvirus / genetics; Potexvirus / physiology; Protoplasts / virology; RNA, Viral / chemistry; RNA, Viral / metabolism; Regulatory Sequences, Nucleic Acid; Templates, Genetic; Tobacco / cytology; Tobacco / virology; Transcription, Genetic; Virulence / genetics; Virus Replication
TL;DR:
It is indicated that long distance RNA-RNA interactions and the sequences of the interacting elements are required for PVX plus-strand RNA accumulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
Restoration of a stem-loop structure required for potato virus X RNA accumulation indicates selection for a mismatch and a GNRA tetraloop
VIROLOGY, 260(2), 342–353.
MeSH headings : Adenosine / genetics; Base Pair Mismatch / genetics; Base Pairing / genetics; Base Sequence; Gene Expression Regulation, Viral; Genome, Viral; Mutagenesis, Site-Directed; Mutation / genetics; Nucleic Acid Conformation; Phenotype; Plants, Toxic; Potexvirus / genetics; Potexvirus / growth & development; Protoplasts / virology; RNA, Messenger / chemistry; RNA, Messenger / genetics; RNA, Messenger / metabolism; RNA, Viral / biosynthesis; RNA, Viral / chemistry; RNA, Viral / genetics; RNA, Viral / metabolism; Thermodynamics; Tobacco / cytology; Tobacco / virology; Virus Replication / genetics
TL;DR:
Data indicate that important sequence and secondary structural elements within SL1 are required for efficient viral infection and that multiple A insertions within the TL and loop C regions, potentially by polymerase stuttering, accompany restoration of SL1 structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018
1998 chapter
History of coat protein-mediated protection
In Plant virology protocols: from virus isolation to transgenic resistance / edited by Gary D. Foster and Sally C. Taylor. (Methods in molecular biology (Clifton, N.J.) ; 81) (Vol. 81, pp. 25–38).
MeSH headings : Capsid Proteins; Genes, Viral; Plant Diseases; Plants / genetics; Plants / immunology; Plants / virology; Plants, Genetically Modified; Plants, Toxic; Tobacco / genetics; Tobacco / virology; Tobacco Mosaic Virus / genetics; Tobacco Mosaic Virus / physiology; Viral Proteins / genetics; Viral Proteins / physiology
TL;DR:
A decade of research has proven that plants can be genetically engineered to resist virus infection through expression of viral CP genes, as well as other viral genes and sequences.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
1998 journal article
Stem-loop structure in the 5 ' region of potato virus X genome required for plus-strand RNA accumulation
JOURNAL OF MOLECULAR BIOLOGY, 284(3), 591–608.
author keywords: RNA structure; RNA virus; RNA replication; potato virus X; stem-loop
MeSH headings : Base Sequence; Genome, Viral; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Plants, Toxic; Potexvirus / genetics; Protoplasts / virology; RNA, Viral / chemistry; RNA, Viral / metabolism; Thermodynamics; Tobacco / metabolism; Tobacco / virology
TL;DR:
Data indicate that multiple features of SL1 are critical for accumulation of PVX plus-strand RNA, including a UUCG tetraloop, indicating that the sequence of this element was important beyond just providing stabilization of the structure.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1997 journal article
Mutations that alter a conserved element upstream of the potato virus X triple block and coat protein genes affect subgenomic RNA accumulation
VIROLOGY, 232(1), 187–197.
MeSH headings : Base Sequence; Capsid / genetics; Conserved Sequence; Genome, Viral; Kinetics; Molecular Sequence Data; Mutation; Potexvirus / genetics; Protoplasts / metabolism; RNA, Messenger / genetics; RNA, Viral / genetics; RNA, Viral / metabolism
TL;DR:
Studies involving inoculation of tobacco plants with the modified transcripts suggested that the conserved octanucleotide element functions in sgRNA accumulation and some other aspect of the infection process.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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