Works (2)

Updated: July 5th, 2023 15:36

2019 journal article

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator


By: A. Westbrook*, X. Tang*, R. Marshall*, C. Maxwell n, J. Chappell*, D. Agrawal*, M. Dunlop*, V. Noireaux* ...

author keywords: Bayesian methods; CRISPRi; model-guided design; RNA-based circuits; sRNA
MeSH headings : CRISPR-Cas Systems; Cell-Free System / chemistry; Cell-Free System / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats; Models, Chemical; RNA / chemistry; RNA / metabolism; Transcription, Genetic; Transcriptional Activation
TL;DR: This work individually characterize two modes of RNA-based transcriptional regulation, using small transcription activating RNAs (STARs) and CRISPR interference (CRISPRi), and shows how their distinct regulatory timescales can be used to engineer a composed feedforward loop that creates a pulse of gene expression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: April 15, 2019

2017 journal article

Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems


By: R. Marshall*, C. Maxwell n, S. Collins n, C. Beisel n & V. Noireaux*

author keywords: Escherichia coli; gene circuits; prototyping; RecBCD; synthetic biology; TXTL
MeSH headings : Cell-Free System / physiology; DNA, Bacterial / genetics; Escherichia coli / genetics; Exodeoxyribonuclease V / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Protein Biosynthesis / genetics; Transcription, Genetic / genetics
TL;DR: It is shown that double-stranded DNA encoding χ sites-eight base-pair sequences preferentially bound by the RecBCD recombination machinery-stabilizes linear DNA and greatly enhances the TXTL-based expression and activity of a fluorescent reporter gene, simple regulatory cascades, and T7 bacteriophage particles. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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