@article{grace_warlick_neff_lila_2014, title={Efficient preparative isolation and identification of walnut bioactive components using high-speed counter-current chromatography and LC-ESI-IT-TOF-MS}, volume={158}, ISSN={["1873-7072"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84896514682&partnerID=MN8TOARS}, DOI={10.1016/j.foodchem.2014.02.117}, abstractNote={Preparative isolation of complex mixtures of compounds from walnut polar extracts was established by a combination of high-speed counter-current chromatography (HSCCC) and electrospray ionization-ion trap-time of flight mass spectrometry (ESI-IT-TOF-MS). Compounds were isolated after a solvent optimisation selection based on solute distribution in a biphasic solvent system. Isolation was achieved through one or two successive HSCCC runs, and final purification on Sephadex LH-20. Isolated compounds included ellagitannins (1–11), gallic acid (12), dicarboxylic acid glucosides (13–15), hydrojuglone glucoside (16), catechin (17), procyanidin B2 (18), and megasterone glucosides (19–20). Praecoxin D (4) was isolated for the first time from walnut, while praecoxin A methyl ester (5) and glansreginin A n-butyl ester (14) are newly identified compounds. The purity and identity of isolated compounds were confirmed by NMR and HPLC-ESI-MS/MS. These results provided a foundation for in depth characterisation of walnut compounds and offered an efficient strategy for isolation of potentially health-relevant phytochemicals from walnuts.}, journal={FOOD CHEMISTRY}, author={Grace, Mary H. and Warlick, Charles W. and Neff, Scott A. and Lila, Mary Ann}, year={2014}, month={Sep}, pages={229–238} }