David A. Zaharoff

855 Interim results from a comparative oncology safety study evaluating intravesical chitosan/interleukin-12 immunotherapy in client-owned dogs with invasive urothelial carcinoma

Helein, G. T., Nguyen, T. T., Intile, J. L., Mastromauro, M. L., Vaden, S. A., Hess, P. R., & Zaharoff, D. A. (2025, November 1). Regular and Young Investigator Award Abstracts, Vol. 11.

884 Localized IL-12 immunotherapy via co-formulation in an injectable hydrogel

Helein, G. T., Mantooth, S. M., Nguyen, T. T., & Zaharoff, D. A. (2025, November 1). Regular and Young Investigator Award Abstracts.

An injectable hydrogel enhances intratumoral retention and antitumor efficacy of cytokine immunotherapy in murine triple negative breast tumor models

Mantooth, S. M., Green, J. M., Green, W. D., Nguyen, K. G., Mantooth, K. A., Meritet, D. M., … Zaharoff, D. A. (2025, April 25). Journal of Controlled Release.

Assessing mutation-origin neopeptide prediction tools in canine cancer using the model MHC class Ia allomorphs, DLA-88*508:01 and *034:01 9302

Hess, P. R., Lee, C.-Y., Holmes, J., & Zaharoff, D. (2025, November 1). The Journal of Immunology.

Chitosan–Glycerol Injectable Hydrogel for Intratumoral Delivery of Macromolecules

Kobrin, R. L., Mantooth, S. M., Mulry, A. L., Zaharoff, D. J., Zaharoff, D. J., Zaharoff, D. A., & Zaharoff, D. A. (2025, August 2). Gels.

Optimization of Bipolar Microsecond Electric Pulses for DNA Vaccine Delivery

IEEE Transactions on Biomedical Engineering, 1–12.

A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation

Cryobiology, 114, 104844.

Characterization of an Injectable Chitosan Hydrogel for the Tunable, Localized Delivery of Immunotherapeutics

Mantooth, S. M., Hancock, A. M., Thompson, P. M., George, V. P. J., Meritet, D. M., Vrabel, M. R., … Zaharoff, D. A. (2024, January 19). ACS Biomaterials Science & Engineering, Vol. 10, pp. 905–920.

Development of Humanized Antibodies Against Vasoactive Intestinal Polypeptide for Increased T Cell Anti-Leukemia Activity

Adeyeye, J., Giver, C. R., Cohen, J., Wang, Y., Charles, T. P., Nguyen, K. G., … Waller, E. K. (2024, November 5). Blood.

Interleukin-12 decorated nanosized semiflexible Immunofilaments enable directed targeting and augmented IFNγ responses of natural killer cells

Weiss, L., Schluck, M., Classens, R., Jonge, P. K. J. D., Waart, A., Nguyen, K. G., … Hammink, R. (2024, November 9). Acta Biomaterialia.

Intratumoral delivery of immunotherapy to treat breast cancer: current development in clinical and preclinical studies

Mantooth, S. M., Abdou, Y., Saez-Ibañez, A. R., Upadhaya, S., & Zaharoff, D. A. (2024, May 13). Frontiers in Immunology.

Focal Cryo-Immunotherapy with Intratumoral IL-12 Prevents Recurrence of Large Murine Tumors

Vrabel, M. R., Schulman, J. A., Gillam, F. B., Mantooth, S. M., Nguyen, K. G., & Zaharoff, D. A. (2023, April 8). Cancers.

Heparin Affinity-Based IL-4 Delivery to Modulate Macrophage Phenotype and Endothelial Cell Activity In Vitro

ACS Applied Materials & Interfaces, 15(23), 27457–27470.

1371 Identification of vasoactive intestinal peptide (VIP)-specific single-chain antibody fragments (scFvs) via yeast surface display

Zaharoff, D., & Nguyen, K. (2022, November 1). Regular and Young Investigator Award Abstracts.

Intranasal Delivery of Thermostable Subunit Vaccine for Cross-Reactive Mucosal and Systemic Antibody Responses Against SARS-CoV-2

Nguyen, K. G., Mantooth, S. M., Vrabel, M. R., & Zaharoff, D. A. (2022, May 3). Frontiers in Immunology.

Raman spectroscopy reveals phenotype switches in breast cancer metastasis

Paidi, S. K., Troncoso, J. R., Harper, M. G., Liu, Z., Nguyen, K. G., Ravindranathan, S., … Barman, I. (2022, January 1). Theranostics.

259 Injectable chitosan hydrogel for localized delivery of immune checkpoint inhibitors

Mantooth, S., Zaharoff, D., & Mantooth, S. (2021, November 1). Regular and Young Investigator Award Abstracts.

620 Onco-immunological mechanisms of focal ablation and localized IL-12 immunotherapy

Vrabel, M., Zaharoff, D., & Mantooth, S. (2021, November 1). Regular and Young Investigator Award Abstracts.

Safety and Pharmacokinetics of Intravesical Chitosan/Interleukin-12 Immunotherapy in Murine Bladders

Nguyen, K. G., Wagner, E. S., Vrabel, M. R., Mantooth, S. M., Meritet, D. M., & Zaharoff, D. A. (2021, October 25). Bladder Cancer.

457 Intratumoral interleukin-12 administered after cryoablation does not improve survival in multiple bilateral murine models

Vrabel, M., Schulman, J., & Zaharoff, D. (2020, November 1). Regular and Young Investigator Award Abstracts.

Construction of heparan sulfate microarray for investigating the binding of specific saccharide sequences to proteins

Horton, M., Su, G., Yi, L., Wang, Z., Xu, Y., Pagadala, V., … Liu, J. (2020, July 15). Glycobiology.

Design of a thrombin resistant human acidic fibroblast growth factor (hFGF1) variant that exhibits enhanced cell proliferation activity

Kerr, R., Agrawal, S., Maity, S., Koppolu, B., Jayanthi, S., Kumar, G. S., … Kumar, T. K. S. (2019, August 13). Biochemical and Biophysical Research Communications.

Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia

Yao, M., Rabbani, Z. N., Sattler, T., Nguyen, K. G., Zaharoff, D. A., Walker, G., & Gamcsik, M. P. (2019, November 7). Analytical Chemistry.

Molecular mechanisms of heparin-induced modulation of human interleukin 12 bioactivity

Journal of Biological Chemistry, 294(12), 4412–4424.

Ultrasound-Stimulated Phase-Change Contrast Agents for Transepithelial Delivery of Macromolecules, Toward Gastrointestinal Drug Delivery

Fix, S. M., Koppolu, B. P., Novell, A., Hopkins, J., Kierski, T. M., Zaharoff, D. A., … Papadopoulou, V. (2019, April 16). Ultrasound in Medicine & Biology.

Analyzing the effects of instillation volume on intravesical delivery using biphasic solute transport in a deformable geometry

Smith, S. G., Griffith, B. E., & Zaharoff, D. A. (2018, March 22). Mathematical Medicine and Biology A Journal of the IMA.

Probing the role of proline −135 on the structure, stability, and cell proliferation activity of human acidic fibroblast growth factor

Davis, J. E., Alghanmi, A., Gundampati, R. K., Jayanthi, S., Fields, E., Armstrong, M., … Kumar, T. K. S. (2018, July 19). Archives of Biochemistry and Biophysics.

Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines

Ravindranathan, S., Nguyen, K. G., Kurtz, S. L., Frazier, H. N., Smith, S. G., Koppolu, B., … Zaharoff, D. A. (2018, October 22). Breast Cancer Research.

Effect of extension of the heparin binding pocket on the structure, stability, and cell proliferation activity of the human acidic fibroblast growth factor

Biochemistry and Biophysics Reports, 13, 45–57.

Modulation of Interleukin-12 activity in the presence of heparin

Jayanthi, S., Koppolu, B., Nguyen, K. G., Smith, S. G., Felber, B. K., Kumar, T. K. S., & Zaharoff, D. A. (2017, July 7). Scientific Reports.

Strategic Directions in Immunoresponsive Biomaterials in Tissue Engineering

Zaharoff, D. A., & Jewell, C. M. (2017, October 1). Tissue Engineering Part A.

Immunological mechanisms of intravesical chitosan/interleukin-12 immunotherapy against murine bladder cancer

Smith, S. G., Baltz, J. L., Koppolu, B. P., Ravindranathan, S., Nguyen, K., & Zaharoff, D. A. (2016, November 18). OncoImmunology.

Corrigendum to ‘Controlling chitosan-based encapsulation for protein and vaccine delivery’ [Biomaterials 35 (2014) 4382–4389]

Biomaterials, 73, 308.

Intravesical chitosan/interleukin-12 immunotherapy induces tumor-specific systemic immunity against murine bladder cancer

Cancer Immunology, Immunotherapy, 64(6), 689–696.

Controlling chitosan-based encapsulation for protein and vaccine delivery

Biomaterials, 35(14), 4382–4389.

Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag

Protein Expression and Purification, 102, 76–84.

583 INTRAVESICAL IMMUNOTHERAPY WITH CHITOSAN AND INTERLEUKIN-12 INDUCES SYSTEMIC TUMOR-SPECIFIC IMMUNITY

Journal of Urology, 189(4S).

Nanotheranostics of Circulating Tumor Cells, Infections and Other Pathological Features in Vivo

Molecular Pharmaceutics, 10(3), 813–830.

Role of chitosan co-formulation in enhancing interleukin-12 delivery and antitumor activity

Biomaterials, 34(15), 3828–3836.

The effect of antigen encapsulation in chitosan particles on uptake, activation and presentation by antigen presenting cells

Biomaterials, 34(9), 2359–2369.

In vivo efficacy of a chitosan/IL-12 adjuvant system for protein-based vaccines

Biomaterials, 32(3), 926–932.

Exercise enhances vaccine-induced antigen-specific T cell responses

Vaccine, 26(42), 5407–5415.

Chitosan solution enhances the immunoadjuvant properties of GM-CSF

Vaccine, 25(52), 8673–8686.

Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo

Bioelectrochemistry, 71(2), 233–242.

A single molecule detection method for understanding mechanisms of electric field-mediated interstitial transport of genes

Bioelectrochemistry, 69(2), 248–253.

Chitosan solution enhances both humoral and cell-mediated immune responses to subcutaneous vaccination

Vaccine, 25(11), 2085–2094.

Electric Fields in Tumors Exposed to External Voltage Sources: Implication for Electric Field-Mediated Drug and Gene Delivery

Annals of Biomedical Engineering, 34(10), 1564–1572.

Electric field-mediated enhancement of in vivo transport of plasmid DNA in tumor interstitium

Journal of Biomechanics, 39, S377.

Nonlinear Dependence of Hydraulic Conductivity on Tissue Deformation During Intratumoral Infusion

Annals of Biomedical Engineering, 34(7), 1173–1181.

Effects of pulse strength and pulse duration on in vitro DNA electromobility

Bioelectrochemistry, 62(1), 37–45.