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Works (41)
Updated: April 5th, 2024 09:41
2024 article
A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation
Vrabel, M. R., Fesmire, C. C., Rich, M. J., Kobrin, R. L., Sano, M. B., & Zaharoff, D. A. (2024, March). CRYOBIOLOGY, Vol. 114.
author keywords: Cryoablation; Focal ablation; Prostate cancer; Clinical ablation; Immunogenic cell death; Transition zone; Cryosurgery
TL;DR:
A longer freezing duration, higher freezing intensity, and more freeze thaw cycles led to higher levels of cancer cell death and smaller transition zones, which have the potential to inform future preclinical research and to improve therapeutic combinations with CA.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Web Of Science
Added: March 4, 2024
2024 journal article
Characterization of an Injectable Chitosan Hydrogel for the Tunable, Localized Delivery of Immunotherapeutics
ACS BIOMATERIALS SCIENCE & ENGINEERING, 10(2), 905–920.
author keywords: hydrogel; chitosan; immunotherapy; intratumoral
TL;DR:
The goal of the present work was to construct a novel, injectable hydrogel that could be tuned for localized immunotherapy delivery and displayed several clinically important features, including injectability, biocompatibility, and imageability.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: March 4, 2024
2023 journal article
Focal Cryo-Immunotherapy with Intratumoral IL-12 Prevents Recurrence of Large Murine Tumors
CANCERS, 15(8).
author keywords: focal ablation; cryoablation; IL-12; intratumoral; immunotherapy; abscopal response; checkpoint therapy
TL;DR:
Cryo-immunotherapy with localized CS/IL-12 reduces recurrences and enhances the elimination of large primary tumors, and induces significant but limited systemic antitumor immunity.
(via Semantic Scholar)
www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 15, 2023
2023 journal article
Heparin Affinity-Based IL-4 Delivery to Modulate Macrophage Phenotype and Endothelial Cell Activity In Vitro
ACS APPLIED MATERIALS & INTERFACES, 15(23), 27457–27470.
author keywords: gelatin methacryloyl; heparin; interleukin4; hydrogel; macrophage; endothelial cell; immunomodulation
MeSH headings : Interleukin-4 / pharmacology; Heparin / pharmacology; Heparin / metabolism; Macrophages / metabolism; Phenotype; Hydrogels / pharmacology; Hydrogels / metabolism
TL;DR:
A gelatin-heparin-methacrylate hydrogel is engineered that gradually releases interleukin-4 (IL-4), a cytokine that modulates macrophages to adopt the M2 phenotype and synergistically downregulated M1 gene expression and upregulated M2 gene expression within 48 h of in vitro cell culture.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 26, 2023
2022 article
IDENTIFICATION OF VASOACTIVE INTESTINAL PEPTIDE (VIP)-SPECIFIC SINGLE-CHAIN ANTIBODY FRAGMENTS (SCFVS) VIA YEAST SURFACE DISPLAY
Zaharoff, D., & Nguyen, K. (2022, November). JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 10, pp. A1424–A1425.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: April 24, 2023
2022 journal article
Intranasal Delivery of Thermostable Subunit Vaccine for Cross-Reactive Mucosal and Systemic Antibody Responses Against SARS-CoV-2
FRONTIERS IN IMMUNOLOGY, 13.
author keywords: Intranasal vaccination; COVID-19; mucosal immunity; SARS-CoV-2; Receptor binding domain (RBD); chitosan; CpG
MeSH headings : Animals; Antibody Formation; COVID-19 / prevention & control; COVID-19 Vaccines; Humans; Immunoglobulin G; Mice; SARS-CoV-2; Vaccines, Subunit
TL;DR:
Data indicate that the designed vaccine platform is versatile, adaptable and capable of overcoming key constraints of current COVID-19 vaccines.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: May 31, 2022
2022 journal article
Raman spectroscopy reveals phenotype switches in breast cancer metastasis
THERANOSTICS, 12(12), 5351–5363.
author keywords: Raman spectroscopy; Cancer metastasis; Random forests; Phenotype switch; TWIST
MeSH headings : Animals; Cell Line, Tumor; Melanoma; Mice; Neoplasm Metastasis; Neoplasms, Second Primary; Phenotype; Skin Neoplasms; Spectrum Analysis, Raman
TL;DR:
The findings indicate that Raman spectroscopy may offer a novel strategy to evaluate metastatic risk during primary tumor biopsies in clinical patients and provide evidence that machine learning models built on spectral data can allow the accurate identification of metastatic phenotype of independent test tumors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 1, 2022
2021 journal article
Construction of heparan sulfate microarray for investigating the binding of specific saccharide sequences to proteins
GLYCOBIOLOGY, 31(3), 188–199.
author keywords: carbohydrates; chemoenzymatic synthesis; heparan sulfate; microarray; oligosaccharides
MeSH headings : Antithrombin III / chemistry; Binding Sites; Carbohydrate Conformation; Carbohydrate Sequence; Fibroblast Growth Factor 2 / chemistry; Heparitin Sulfate / chemistry; Humans; Microarray Analysis
TL;DR:
A microarray of diverse chemoenzymatically synthesized HS structures was developed and HS interactions were characterized and specific sulfation patterns were found to be important for binding to these proteins and results are consistent with previous specificity studies.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: May 24, 2021
2021 article
INJECTABLE CHITOSAN HYDROGEL FOR LOCALIZED DELIVERY OF IMMUNE CHECKPOINT INHIBITORS
Mantooth, S., Zaharoff, D., & Mantooth, S. (2021, November). JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 9, pp. A281–A281.
TL;DR:
A novel injectable chitosan hydrogel was found to provide sustained release of a large model protein over a 1–2 week period with favorable in vitro kinetics and can be engineered to provide faster or slower release as needed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: April 18, 2022
2021 article
ONCO-IMMUNOLOGICAL MECHANISMS OF FOCAL ABLATION AND LOCALIZED IL-12 IMMUNOTHERAPY
Vrabel, M., Zaharoff, D., & Mantooth, S. (2021, November). JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 9, pp. A650–A650.
TL;DR:
In the spontaneously metastatic LLC model, delivering CS/IL-12 two days before treatment, either resection or cryoablation, reduced the number of metastatic lung nodules and furthermore prevented the recurrence of the primary tumor after resection.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: April 18, 2022
2021 journal article
Safety and Pharmacokinetics of Intravesical Chitosan/Interleukin-12 Immunotherapy in Murine Bladders
BLADDER CANCER, 7(4), 427–437.
author keywords: Bladder cancer; Interleukin-12; immunotherapy; chitosan; intravesical
TL;DR:
The lack of cystitis and acute inflammation justifies continued investigation of intravesical CS/IL-12 immunotherapy in larger animals and patients with bladder cancer.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: January 10, 2022
2020 article
INTRATUMORAL INTERLEUKIN-12 ADMINISTERED AFTER CRYOABLATION DOES NOT IMPROVE SURVIVAL IN MULTIPLE BILATERAL MURINE MODELS
Vrabel, M., Schulman, J., & Zaharoff, D. (2020, November). JOURNAL FOR IMMUNOTHERAPY OF CANCER, Vol. 8, pp. A277–A278.
TL;DR:
This study hypothesized that intratumoral injection of IL-12 after cryo would activate a strong T cell response and induce systemic immunity in bilateral tumor models and indicated that the addition of interleukin-12 to cryoablation (cryo) improved tumor burden and survival.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: March 1, 2021
2019 journal article
Analyzing the effects of instillation volume on intravesical delivery using biphasic solute transport in a deformable geometry
MATHEMATICAL MEDICINE AND BIOLOGY-A JOURNAL OF THE IMA, 36(2), 139–156.
author keywords: bladder cancer; cystitis; intravesical delivery; FEBio
MeSH headings : Administration, Intravesical; Humans; Models, Theoretical; Urinary Bladder Diseases / drug therapy
TL;DR:
The volume of instillation was found to have a substantial impact on the exposure of solute to the deeper muscle layers of the bladder, which are typically more difficult to reach and indicate the role that instillation volume may play in targeting particular layers ofThe bladder during an intravesical delivery.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
5. Gender Equality
(Web of Science)
Source: Web Of Science
Added: November 18, 2019
2019 journal article
Design of a thrombin resistant human acidic fibroblast growth factor (hFGF1) variant that exhibits enhanced cell proliferation activity
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 518(2), 191–196.
author keywords: Fibroblast growth factor; Cell signaling; Stability; Heparin; Binding affinity; Cell proliferation
MeSH headings : Animals; Cell Proliferation; Fibroblast Growth Factor 1 / chemistry; Fibroblast Growth Factor 1 / genetics; Fibroblast Growth Factor 1 / metabolism; Humans; Mice; Models, Molecular; Mutagenesis, Site-Directed; NIH 3T3 Cells; Point Mutation; Protein Conformation; Thrombin / metabolism
TL;DR:
The R136E variant due to its increased thermal stability, resistance to proteases, and enhanced cell proliferation activity are expected to provide valuable clues for the development of hFGF1- based therapeutics for the management of chronic diabetic wounds.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: January 21, 2020
2019 journal article
Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia
ANALYTICAL CHEMISTRY, 91(23), 15032–15039.
MeSH headings : Aldehyde Dehydrogenase / metabolism; Caco-2 Cells; Cell Hypoxia; Cell Line, Tumor; Glucose / pharmacokinetics; Humans; Hypoxia-Inducible Factor 1, alpha Subunit / drug effects; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Oxygen / metabolism; Oxygen / pharmacology; Pancreatic Neoplasms / pathology; Time Factors
TL;DR:
A manifold designed to use flow-encoding to produce sequential changes in gas mixtures delivered to a permeable-bottom 96-well plate is fabricated and shown how this manifold and plate design can be used to expose cells to either static or cycling hypoxic conditions for eight different time periods thereby facilitating the study of the time-response of cells to altered oxygen environments.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: December 30, 2019
2019 journal article
Molecular mechanisms of heparin-induced modulation of human interleukin 12 bioactivity
Journal of Biological Chemistry, 294(12), 4412–4424.
author keywords: heparin; interleukin; glycosaminoglycan; cytokine; immunology; heparan sulfate; heparin-binding cytokine; IL-12
MeSH headings : Animals; Biophysical Phenomena; Calorimetry; Cytokines / biosynthesis; Dose-Response Relationship, Drug; Female; Flow Cytometry; HEK293 Cells; Heparin / chemistry; Heparin / pharmacology; Heparitin Sulfate / metabolism; Humans; Interleukin-12 / metabolism; Interleukin-12 / pharmacology; Mice; Mice, Inbred C57BL; Protein Binding; Receptors, Interleukin-2 / metabolism; Recombinant Proteins / metabolism; Recombinant Proteins / pharmacology; Signal Transduction / drug effects; T-Lymphocytes / drug effects; T-Lymphocytes / metabolism
TL;DR:
Heparin-dependent modulation of hIL-12 function correlates with several of heparin's biophysical characteristics, including chain length, sulfation level, and concentration, which provide a foundation for further investigation of heParin's interactions with IL-12 family cytokines and for the use of he parin as an immunomodulatory agent.
(via Semantic Scholar)
Source: Crossref
Added: February 24, 2020
2019 journal article
ULTRASOUND-STIMULATED PHASE-CHANGE CONTRAST AGENTS FOR TRANSEPITHELIAL DELIVERY OF MACROMOLECULES, TOWARD GASTROINTESTINAL DRUG DELIVERY
ULTRASOUND IN MEDICINE AND BIOLOGY, 45(7), 1762–1776.
author keywords: Ultrasound; Transepithelial delivery; Acoustic droplet vaporization; Phase-change contrast agents; Nanodroplet
MeSH headings : Cells, Cultured; Contrast Media; Dextrans / administration & dosage; Drug Delivery Systems / methods; Fluorescein-5-isothiocyanate / administration & dosage; Fluorescein-5-isothiocyanate / analogs & derivatives; Gastrointestinal Agents / administration & dosage; Humans; Image Enhancement / methods; In Vitro Techniques; Ultrasonography / methods
TL;DR:
It is demonstrated that ultrasound-stimulated phase change contrast agents (PCCAs) can transiently disrupt confluent colorectal adenocarcinoma monolayers and improve the transepithelial transport of a macromolecular model drug.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: June 24, 2019
2018 journal article
Effect of extension of the heparin binding pocket on the structure, stability, and cell proliferation activity of the human acidic fibroblast growth factor
Biochemistry and Biophysics Reports, 13, 45–57.
author keywords: Fibroblast growth factor; Heparin binding; Stability; Cell proliferation
TL;DR:
The results of this study clearly demonstrate that heparin binding affinity of hFGF1 is not strongly correlated to its cell proliferation activity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: February 24, 2020
2018 journal article
Probing the role of proline-135 on the structure, stability, and cell proliferation activity of human acidic fibroblast growth factor
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 654, 115–125.
author keywords: Fibroblast growth factor; Heparin binding; Charge-reversal; Bioactivity; Stability
MeSH headings : Cell Proliferation / physiology; Fibroblast Growth Factor 1 / chemistry; Fibroblast Growth Factor 1 / genetics; Fibroblast Growth Factor 1 / physiology; Heparin / metabolism; Humans; Mutagenesis, Site-Directed; Proline / physiology; Protein Binding; Protein Stability; Protein Structure, Tertiary; Proton Magnetic Resonance Spectroscopy; Receptors, Fibroblast Growth Factor / metabolism
TL;DR:
The results of this study suggest that while heparin is useful for stabilizing hFGF1 on the cell surface, this interaction is not mandatory for activation of the FGF receptor.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: October 19, 2018
2018 journal article
Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines
BREAST CANCER RESEARCH, 20.
author keywords: Breast cancer vaccine; Autologous tumor cell vaccine; MDSCs; Breast cancer immunogenicity
MeSH headings : Animals; Breast / pathology; Breast Neoplasms / immunology; Breast Neoplasms / pathology; Breast Neoplasms / therapy; CRISPR-Cas Systems; Cancer Vaccines / administration & dosage; Cancer Vaccines / immunology; Cell Line, Tumor / immunology; Cell Line, Tumor / radiation effects; Cell Line, Tumor / transplantation; Disease Models, Animal; Female; Gene Deletion; Granulocyte Colony-Stimulating Factor / genetics; Granulocyte Colony-Stimulating Factor / immunology; Humans; Immunogenicity, Vaccine; Mice; Mice, Inbred BALB C; Neoplasm Recurrence, Local / immunology; Neoplasm Recurrence, Local / prevention & control; Treatment Outcome
TL;DR:
This study demonstrates that genetic ablation of immunosuppressive cytokines, such as G-CSF, can enhance the immunogenicity of breast cancer cell-based vaccines.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: November 12, 2018
2017 journal article
Immunological mechanisms of intravesical chitosan/interleukin-12 immunotherapy against murine bladder cancer
ONCOIMMUNOLOGY, 6(1).
author keywords: BCG; bladder cancer; chitosan; immunotherapy; interleukin-12; intratumoral; intravesical; MDSC; TILS; TReg
TL;DR:
The ability of properly delivered interleukin-12-based therapies to engage adaptive immunity within the tumor itself as well as throughout the body is demonstrated and the case for clinical translation of chitosan/interleuk in-12 as an intravesical treatment for bladder cancer is strengthened.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2017 journal article
Modulation of Interleukin-12 activity in the presence of heparin
SCIENTIFIC REPORTS, 7.
MeSH headings : Biophysical Phenomena; Cell Line; Chromatography, Gel; Flow Cytometry; Heparin / metabolism; Heparitin Sulfate / metabolism; Humans; Immunologic Factors / metabolism; Interleukin-12 / chemistry; Interleukin-12 / metabolism; Interleukin-12 Subunit p35; Protein Binding; Protein Conformation / drug effects; Protein Multimerization / drug effects; Scattering, Small Angle
TL;DR:
Results of this study demonstrate a new role for heparin in modulating the biological activity of IL-12, and Heparin modestly protected hIL-12 from proteolytic degradation, however, this was not a likely mechanism for increased cytokine activity in vitro.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 article
Strategic Directions in Immunoresponsive Biomaterials in Tissue Engineering INTRODUCTION
Zaharoff, D. A., & Jewell, C. M. (2017, October). TISSUE ENGINEERING PART A, Vol. 23, pp. 1042–1043.
MeSH headings : Biocompatible Materials / adverse effects; Biocompatible Materials / chemistry; Cell Survival / drug effects; Humans; Regenerative Medicine / methods; Tissue Engineering / methods
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2015 journal article
Corrigendum to ‘Controlling chitosan-based encapsulation for protein and vaccine delivery’ [Biomaterials 35 (2014) 4382–4389]
Biomaterials, 73, 308.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2015 journal article
Intravesical chitosan/interleukin-12 immunotherapy induces tumor-specific systemic immunity against murine bladder cancer
Cancer Immunology, Immunotherapy, 64(6), 689–696.
author keywords: Intravesical immunotherapy; Bladder cancer; Interleukin-12; Chitosan
MeSH headings : Administration, Intravesical; Animals; Cell Line, Tumor; Chitosan / administration & dosage; Chitosan / immunology; Dose-Response Relationship, Immunologic; Female; Immunotherapy / methods; Interleukin-12 / administration & dosage; Interleukin-12 / immunology; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Random Allocation; Urinary Bladder Neoplasms / drug therapy; Urinary Bladder Neoplasms / immunology
TL;DR:
In an advanced bladder cancer model, intravesical CS/IL-12 immunotherapy controlled simultaneous orthotopic and subcutaneous tumors in 70 % of treated mice and Protective immunity was durable, lasting for at least 18 months after immunotherapy.
(via Semantic Scholar)
Source: Crossref
Added: February 24, 2020
2014 journal article
Controlling chitosan-based encapsulation for protein and vaccine delivery
Biomaterials, 35(14), 4382–4389.
author keywords: Chitosan particles; Protein encapsulation; Vaccine delivery; Precipitation-coacervation; Microencapsulation; Nanoencapsulation
MeSH headings : Calorimetry; Chemical Precipitation; Chitosan / chemistry; Drug Delivery Systems; Fluorescein-5-isothiocyanate / administration & dosage; Fluorescein-5-isothiocyanate / analogs & derivatives; Fluorescein-5-isothiocyanate / chemistry; Immobilized Proteins / chemistry; Molecular Weight; Osmolar Concentration; Particle Size; Protein Binding; Serum Albumin, Bovine / administration & dosage; Serum Albumin, Bovine / chemistry; Solutions; Sonication; Sulfates / chemistry; Thermodynamics; Vaccines / administration & dosage; Water / chemistry
TL;DR:
It is demonstrated that chitosan nano/microparticles with high protein loading efficiencies can be engineered with well-defined sizes and controllable release kinetics through manipulation of specific formulation parameters.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2014 journal article
Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag
Protein Expression and Purification, 102, 76–84.
author keywords: Interleukin-12; Heparin Sepharose; HEK293; Cytokine; Purification; Bioactivity
MeSH headings : Amino Acid Sequence; Binding Sites; Blotting, Western; Chromatography, Affinity; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; HEK293 Cells / metabolism; Heparin / metabolism; Humans; Interleukin-12 / chemistry; Interleukin-12 / genetics; Interleukin-12 / isolation & purification; Interleukin-12 / metabolism; Models, Molecular; Molecular Sequence Data; Protein Conformation; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Up-Regulation
TL;DR:
The purification method described in this study is expected to provide greater impetus for research on the role of heparin in the regulation of the function of IL-12 and provide an avenue to obtain high amounts ofIL-12 required for structural studies which are aimed at the development of novel IL- 12-based therapeutics.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2013 journal article
583 INTRAVESICAL IMMUNOTHERAPY WITH CHITOSAN AND INTERLEUKIN-12 INDUCES SYSTEMIC TUMOR-SPECIFIC IMMUNITY
Journal of Urology, 189(4S).
Source: Crossref
Added: August 28, 2020
2013 journal article
Nanotheranostics of Circulating Tumor Cells, Infections and Other Pathological Features in Vivo
Molecular Pharmaceutics, 10(3), 813–830.
author keywords: metastasis; circulating tumor cells (CTCs); circulating endothelial cells (CECs); circulating cancer stem cells (CCSCs); circulating microparticles and exosomes; circulating pathogens; viruses and parasites; blood clots; blood and lymph flow; contrast nanoagents; positive and negative photoacoustic contrasts; micro- and nanobubbles; multimodal; multicolor; nanotechnology; multifunctional real-time nanotheranostics; nanomedicine
MeSH headings : Humans; Molecular Imaging / methods; Nanotechnology / methods; Neoplastic Cells, Circulating / pathology; Photoacoustic Techniques
TL;DR:
An urgent need is emphasized for in vivo platforms composed of multifunctional contrast nanoagents, which utilize diverse modalities to realize a breakthrough for early detection and treatment of harmful diseases disseminated through the circulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: October 29, 2020
2013 journal article
Role of chitosan co-formulation in enhancing interleukin-12 delivery and antitumor activity
Biomaterials, 34(15), 3828–3836.
author keywords: Chitosan; Paracrine delivery; Interleukin-12; Intratumoral; Immunotherapy
MeSH headings : Animals; Antineoplastic Agents / administration & dosage; Antineoplastic Agents / pharmacology; Cell Line, Tumor; Cell Proliferation / drug effects; Chemistry, Pharmaceutical; Chitosan / chemistry; Dose-Response Relationship, Drug; Drug Delivery Systems; Female; Humans; Injections; Interleukin-12 / administration & dosage; Interleukin-12 / pharmacology; Leukocytes / cytology; Leukocytes / drug effects; Mice; Mice, Inbred C57BL; Phenotype
TL;DR:
It is suggested that chitosan-mediated depot formation and enhanced local cytokine retention is significantly, but not entirely, responsible for increased cytokine bioactivity.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2013 journal article
The effect of antigen encapsulation in chitosan particles on uptake, activation and presentation by antigen presenting cells
Biomaterials, 34(9), 2359–2369.
author keywords: Chitosan particles; Antigen delivery; Antigen presentation; Adjuvant; Co-stimulation
MeSH headings : Animals; Antigen Presentation; Antigen-Presenting Cells / metabolism; Antigens / chemistry; Biocompatible Materials / chemistry; Cell Line; Cell Proliferation; Chitosan / chemistry; Dendritic Cells / cytology; Dendritic Cells / immunology; Dendritic Cells / metabolism; Macrophages / cytology; Macrophages / immunology; Macrophages / metabolism; Mice; Ovalbumin / chemistry; Particle Size; Serum Albumin / chemistry
TL;DR:
Data suggest that encapsulation of antigens in chitosan particles enhances uptake, activation and presentation by APCs.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2011 journal article
In vivo efficacy of a chitosan/IL-12 adjuvant system for protein-based vaccines
Biomaterials, 32(3), 926–932.
author keywords: Chitosan; Drug delivery; Immunostimulation; Interleukin 12; Polysaccharide; Vaccine
MeSH headings : Adjuvants, Immunologic / metabolism; Animals; CD4-Positive T-Lymphocytes / metabolism; Chitosan / immunology; Enzyme-Linked Immunosorbent Assay; Interferon-gamma / metabolism; Interleukin-12 / immunology; Mice; Mice, Inbred C57BL; Proteins / immunology; Vaccines / immunology
TL;DR:
A vaccine adjuvant system using a viscous chitosan solution and interleukin (IL)-12, a Th1-inducing cytokine, promoted the generation of a Th 1 immune response to a model protein vaccine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2008 journal article
Exercise enhances vaccine-induced antigen-specific T cell responses
Vaccine, 26(42), 5407–5415.
author keywords: adaptive immunity; cytokines; cell proliferation
MeSH headings : Animals; Body Composition; CD4-Positive T-Lymphocytes / immunology; CD4-Positive T-Lymphocytes / metabolism; Cell Proliferation; Cytokines / biosynthesis; Cytokines / immunology; Female; Lymphocyte Activation / immunology; Mice; Mice, Inbred C57BL; Motor Activity; Ovalbumin / immunology; Substrate Specificity; Vaccination
TL;DR:
Coupling moderate exercise with vaccination may enhance vaccine efficacy for the prevention and/or therapy of numerous diseases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2007 journal article
Chitosan solution enhances both humoral and cell-mediated immune responses to subcutaneous vaccination
Vaccine, 25(11), 2085–2094.
author keywords: adjuvant; depot; vaccination
MeSH headings : Adjuvants, Immunologic; Aluminum Hydroxide / immunology; Animals; Antibodies / blood; CD4-Positive T-Lymphocytes / immunology; Chitosan / immunology; Chitosan / pharmacokinetics; Enzyme-Linked Immunosorbent Assay; Female; Freund's Adjuvant; Histocytochemistry; Hypersensitivity, Delayed; Injections, Subcutaneous; Lipids; Lymph Nodes / cytology; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Models, Animal; Skin / pathology; Vaccination; Vaccines / administration & dosage; Vaccines / immunology; beta-Galactosidase / immunology
TL;DR:
Mechanistic studies demonstrate that chitosan is a promising and safe platform for parenteral vaccine delivery and at least two characteristics that may allow it to function as an immune adjuvant.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2007 journal article
Chitosan solution enhances the immunoadjuvant properties of GM-CSF
Vaccine, 25(52), 8673–8686.
author keywords: chitosan; immunoadjuvant; GM-CSF; controlled release; cytokine
MeSH headings : Adjuvants, Immunologic / administration & dosage; Animals; Antigen Presentation / immunology; CD4-Positive T-Lymphocytes / immunology; CD8-Positive T-Lymphocytes / immunology; Cell Proliferation; Chitosan / immunology; Cytotoxicity Tests, Immunologic; Delayed-Action Preparations / administration & dosage; Dendritic Cells / immunology; Female; Gene Expression; Granulocyte-Macrophage Colony-Stimulating Factor / administration & dosage; Granulocyte-Macrophage Colony-Stimulating Factor / immunology; Histocompatibility Antigens Class II / genetics; Injections, Subcutaneous; Lymph Nodes / immunology; Lymphocyte Subsets / immunology; Mice; T-Lymphocytes, Cytotoxic / immunology
TL;DR:
Chitosan/rGM- CSF outperformed standard rGM-CSF administrations in dendritic cell recruitment, antigen presentation and vaccine enhancement and was superior to either chitOSan or rGM -CSF alone in enhancing the induction of antigen-specific CD4(+) proliferation, peptide- specific CD8(+) pentamer staining and cytotoxic T cell lysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2007 journal article
Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo
Bioelectrochemistry, 71(2), 233–242.
author keywords: in vivo DNA electrophoresis; interstitial transport; non-viral gene therapy; DNA electrotransfer; electric field-mediated gene delivery
MeSH headings : Animals; Azo Compounds; Biological Transport, Active; Cell Line, Tumor; Collagen; DNA, Neoplasm / genetics; DNA, Neoplasm / metabolism; Electrodes; Electrophoresis; Eosine Yellowish-(YS); Fluorescent Dyes; Mammary Neoplasms, Experimental / genetics; Mammary Neoplasms, Experimental / metabolism; Melanoma, Experimental / genetics; Melanoma, Experimental / metabolism; Methyl Green; Mice; Microscopy, Confocal; Plasmids / genetics; Plasmids / metabolism; Rhodamines
Source: Crossref
Added: August 28, 2020
2006 journal article
A single molecule detection method for understanding mechanisms of electric field-mediated interstitial transport of genes
Bioelectrochemistry, 69(2), 248–253.
author keywords: single molecule detection; DNA electromobility; interstitial transport; electric field-mediated gene delivery; non-viral gene delivery
MeSH headings : DNA / chemistry; DNA / radiation effects; Dose-Response Relationship, Radiation; Electromagnetic Fields; Electroporation / methods; Molecular Probe Techniques; Motion; Radiation Dosage; Stress, Mechanical; Transfection / methods
TL;DR:
A reproducible single molecule detection method is developed to observe the electromobility of DNA in a range of pulsed, high field strength electric fields typically used during electric field-mediated gene delivery and suggests the existence of a minimum pulse amplitude required to initiate electrophoretic transport.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2006 journal article
Electric Fields in Tumors Exposed to External Voltage Sources: Implication for Electric Field-Mediated Drug and Gene Delivery
Annals of Biomedical Engineering, 34(10), 1564–1572.
author keywords: drug and gene delivery; electroporation; electrogenetherapy; electrochemotherapy
MeSH headings : Animals; Biomedical Engineering; Cell Line, Tumor; Drug Delivery Systems; Electric Impedance; Electric Stimulation; Electrochemotherapy; Electroporation; Female; Genetic Therapy; In Vitro Techniques; Mammary Neoplasms, Experimental / physiopathology; Mammary Neoplasms, Experimental / therapy; Melanoma, Experimental / physiopathology; Melanoma, Experimental / therapy; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Phantoms, Imaging
TL;DR:
The distribution of the electric field in mouse tumors and tissue phantoms exposed to a large range of electric stimuli, and the resistances of tumor, skin, and electrode-tissue interface are investigated to help improve electric field-mediated drug and gene delivery in solid tumors.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2006 journal article
Electric field-mediated enhancement of in vivo transport of plasmid DNA in tumor interstitium
Journal of Biomechanics, 39, S377.
Source: Crossref
Added: August 28, 2020
2006 journal article
Nonlinear Dependence of Hydraulic Conductivity on Tissue Deformation During Intratumoral Infusion
Annals of Biomedical Engineering, 34(7), 1173–1181.
author keywords: collagen concentration; hydraulic conductivity; intratumoral infusion; drug and gene delivery
MeSH headings : Animals; Antineoplastic Agents / administration & dosage; Biological Transport; Cell Line, Tumor; Collagen / metabolism; Elasticity; Gene Transfer Techniques; Genetic Therapy; Humans; Infusions, Parenteral / methods; Mice; Models, Biological; Neoplasm Transplantation; Neoplasms, Experimental / metabolism; Neoplasms, Experimental / therapy
TL;DR:
Results from numerical simulations based on the poroelastic model suggest that both the existence of Pt and the nonlinear relationships between Q and Pinf could be explained by infusion-induced tissue deformation that anisotropically affected the hydraulic conductivity of tissues.
(via Semantic Scholar)
Source: Crossref
Added: August 28, 2020
2004 journal article
Effects of pulse strength and pulse duration on in vitro DNA electromobility
Bioelectrochemistry, 62(1), 37–45.
author keywords: interstitial transport; plasmid electromobility; electric field-mediated gene delivery; non-viral gene delivery
MeSH headings : DNA / chemistry; Electrochemistry; Electrophoresis, Gel, Pulsed-Field / methods; Fluorescence; Gels / chemistry; Gene Transfer Techniques; In Vitro Techniques; Ions / chemistry; Plasmids / chemistry; Plasmids / genetics
TL;DR:
Electric field was better than concentration gradient of DNA as a driving force for interstitial transport of plasmid DNA and suggested that both pulse strength and duration needed to be adequately high for efficient plasmids transport through extracellular matrix.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: Crossref
Added: August 28, 2020
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