Works (53)

Updated: April 4th, 2024 18:59

2023 journal article

Tizoxanide Antiviral Activity on Dengue Virus Replication

VIRUSES-BASEL, 15(3).

By: K. Yamamoto*, K. Blackburn n, M. Goshe n, D. Brown n, E. Migoswski*, I. Campanhon*, M. Moreira*, D. Ferreira n, M. Soares*

author keywords: antiviral; tizoxanide; dengue virus; virucidal activity
MeSH headings : Chlorocebus aethiops; Animals; Humans; Antiviral Agents / pharmacology; Antiviral Agents / therapeutic use; Vero Cells; Dengue / drug therapy; Dengue Virus / genetics; Proteomics; Virus Replication
TL;DR: TIZ is a promising therapeutic molecule for the treatment of DENV-2 infections and the study of the protein profile of infected not-treated and infected-treated Vero cells showed that TIZ interferes with cellular processes such as intracellular trafficking and vesicle-mediated transport and post-translational modifications when added after infection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: April 17, 2023

2018 journal article

Role of the vacuolar ATPase in the Alphavirus replication cycle

Heliyon, 4(7), e00701.

By: R. Schuchman n, R. Vancini n, A. Piper n, D. Breuer n, M. Ribeiro n, D. Ferreira n, J. Magliocca n, V. Emmerich n, R. Hernandez n, D. Brown n

author keywords: Virology; Cell biology
TL;DR: Results show that a functional V-ATPase is required for efficient virus RNA synthesis and virus maturation in Alphavirus infection. (via Semantic Scholar)
Source: Crossref
Added: June 6, 2020

2016 journal article

Single-Site Glycoprotein Mutants Inhibit a Late Event in Sindbis Virus Assembly

JOURNAL OF VIROLOGY, 90(18), 8372–8380.

By: J. Magliocca n, R. Vancini n, R. Hernandez n & D. Brown n

MeSH headings : Animals; Cells, Cultured; Cricetinae; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; Microscopy, Electron; Mutant Proteins / genetics; Mutant Proteins / metabolism; Mutation, Missense; Point Mutation; Sequence Deletion; Sindbis Virus / genetics; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Suppression, Genetic; Temperature; Viral Envelope Proteins / genetics; Viral Envelope Proteins / metabolism; Viral Load; Virion / ultrastructure; Virus Assembly; Virus Cultivation; Virus Release
TL;DR: It is concluded that H230 is essential for the assembly of complete infectious Sindbis virus virions and that the presence of an amino acid at E2 position 209 is required for complete budding of Sind Bis virus particles although several different amino acids can be at this location without affecting the titer. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

The role of the vacuolar ATPase in alphavirus replication.

Molecular Biology of the Cell, 27.

By: R. Schuchman, R. Vancini, A. Piper, D. Breuer, M. Ribeiro, D. Farreira, J. Magliocca, V. Emmerich, R. Hernandez, D. Brown

Source: NC State University Libraries
Added: August 6, 2018

2015 review

Alphavirus Entry into Host Cells

[Review of ]. Molecular basis of viral infection, 129, 33–62.

By: R. Vancini, R. Hernandez & D. Brown

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Live Attenuated Tetravalent Dengue Virus Host Range Vaccine Is Immunogenic in African Green Monkeys following a Single Vaccination

JOURNAL OF VIROLOGY, 88(12), 6729–6742.

By: C. Briggs*, K. Smith*, A. Piper n, E. Huitt*, C. Spears*, M. Quiles*, M. Ribeiro n, M. Thomas*, D. Brown n, R. Hernandez n

MeSH headings : Animals; Antibodies, Viral / immunology; Chlorocebus aethiops; Dengue / immunology; Dengue / prevention & control; Dengue / virology; Dengue Vaccines / administration & dosage; Dengue Vaccines / genetics; Dengue Vaccines / immunology; Dengue Virus / classification; Dengue Virus / genetics; Dengue Virus / immunology; Dengue Virus / physiology; Host Specificity; Humans; Species Specificity; Vaccination; Vaccines, Attenuated / administration & dosage; Vaccines, Attenuated / genetics; Vaccines, Attenuated / immunology; Viral Envelope Proteins / administration & dosage; Viral Envelope Proteins / genetics; Viral Envelope Proteins / immunology
TL;DR: A live attenuated viral (LAV) vaccine capable of eliciting a strong immune response in African green monkeys (AGMs) in a single dose is generated, and this study suggests that viremia and antibody response in AGMs may be similar to those seen in DENV infection in humans. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Alphavirus Genome Delivery Occurs Directly at the Plasma Membrane in a Time- and Temperature-Dependent Process

JOURNAL OF VIROLOGY, 87(8), 4352–4359.

By: R. Vancini n, G. Wang n, D. Ferreira*, R. Hernandez n & D. Brown n

MeSH headings : Animals; Cell Membrane / virology; Cells, Cultured; Cricetinae; Microscopy, Immunoelectron; RNA, Viral / metabolism; Sindbis Virus / physiology; Staining and Labeling / methods; Temperature; Time Factors; Virus Internalization
TL;DR: It is concluded that entry of alphaviruses is by direct penetration of cell plasma membranes through a pore structure formed by virus and, possibly, host proteins. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Chikungunya Virus Host Range E2 Transmembrane Deletion Mutants Induce Protective Immunity against Challenge in C57BL/6J Mice

JOURNAL OF VIROLOGY, 87(12), 6748–6757.

By: A. Piper n, M. Ribeiro n, K. Smith*, C. Briggs*, E. Huitt*, K. Nanda*, C. Spears*, M. Quiles* ...

MeSH headings : Alphavirus Infections / immunology; Alphavirus Infections / prevention & control; Alphavirus Infections / virology; Animals; Antibodies, Neutralizing / blood; Antibodies, Viral / blood; Cell Line; Chikungunya Fever; Chikungunya virus / genetics; Chikungunya virus / immunology; Chikungunya virus / pathogenicity; Chikungunya virus / physiology; Host Specificity; Humans; Mice; Mice, Inbred C57BL; Mutation; Sequence Deletion; Vaccines, Attenuated / genetics; Vaccines, Attenuated / immunology; Viral Vaccines / genetics; Viral Vaccines / immunology; Virus Replication
TL;DR: This study identified a ChikV HR mutant that grows to high levels in insect cells but was restricted in the ability to assemble virus in mammalian cells in vitro. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Flavivirus infection from mosquitoes in vitro reveals cell entry at the plasma membrane

VIROLOGY, 435(2), 406–414.

By: R. Vancini n, L. Kramer*, M. Ribeiro n, R. Hernandez n & D. Brown n

author keywords: Flavivirus; Virus entry; Mosquitoes; Electron microscopy
MeSH headings : Animals; Cell Membrane / ultrastructure; Cell Membrane / virology; Chlorocebus aethiops; Culex / virology; Culicidae / virology; Dengue Virus / pathogenicity; Dengue Virus / ultrastructure; Flavivirus / pathogenicity; Microscopy, Electron; Vero Cells / virology; Virus Internalization; West Nile virus / pathogenicity; West Nile virus / ultrastructure
TL;DR: The results show that Dengue and West Nile viruses may infect cells by a mechanism that involves direct penetration of the host cell plasma membrane as proposed for alphaviruses. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Conformational Changes in Sindbis Virus Induced by Decreased pH Are Revealed by Small-Angle Neutron Scattering

JOURNAL OF VIROLOGY, 86(4), 1982–1987.

By: L. He*, A. Piper n, F. Meilleur n, R. Hernandez n, W. Heller* & D. Brown n

MeSH headings : Hydrogen-Ion Concentration; RNA, Viral / chemistry; RNA, Viral / genetics; RNA, Viral / metabolism; Scattering, Small Angle; Sindbis Virus / chemistry; Sindbis Virus / genetics; Sindbis Virus / metabolism; Viral Proteins / chemistry; Viral Proteins / genetics; Virion / chemistry; Virion / genetics; Virion / metabolism
TL;DR: Small-angle neutron scattering with contrast variation is employed to probe how the structure of a mammalian-grown Sindbis virus responds to moderately acidic pH and improves the understanding of the pH-driven alteration of Sind Bis virus structure. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

Infection of cells by alphaviruses

Viral Molecular Machines, 726, 181–199.

By: D. Brown & R. Hernandez

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Testing of Novel Dengue Virus 2 Vaccines in African Green Monkeys: Safety, Immunogenicity, and Efficacy

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 87(4), 743–753.

By: K. Smith, K. Nanda, V. McCarl, C. Spears, A. Piper*, M. Ribeiro, M. Quiles, C. Briggs ...

MeSH headings : Animals; Antibodies, Neutralizing / blood; Antibodies, Viral / blood; Cells, Cultured; Chlorocebus aethiops; Dengue / immunology; Dengue / prevention & control; Dengue / virology; Dengue Vaccines / adverse effects; Dengue Vaccines / genetics; Dengue Vaccines / immunology; Dengue Vaccines / therapeutic use; Dengue Virus / genetics; Dengue Virus / immunology; Immunization; Neutralization Tests; Viral Envelope Proteins / genetics; Viral Envelope Proteins / immunology; Viral Plaque Assay
TL;DR: Three novel host-range vaccines containing deletions in the transmembrane domain of dengue virus serotype 2 (DV2) E glycoprotein were evaluated in African green monkeys, and all three vaccine strains decreased the length of viremia by at least two days. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Alphavirus adsorption to mosquito cells as viewed by freeze fracture immunolabeling

VIROLOGY, 415(2), 132–140.

By: J. Kononchik n, R. Vancini n & D. Brown n

author keywords: Alphavirus; Cell penetration; Insect cells; Freeze-fracture; Electron microscopy
MeSH headings : Adsorption; Alphavirus / chemistry; Alphavirus / physiology; Alphavirus / ultrastructure; Animals; Cell Membrane / chemistry; Cell Membrane / ultrastructure; Cell Membrane / virology; Cells, Cultured; Culicidae / virology; Freeze Fracturing / methods; Immunohistochemistry / methods
TL;DR: Using freeze-fracture replicas, thin-sections and antibody labeling the data suggest that the intramembrane particles associated with SV may be part of the pore structure consisting of virus proteins and cell receptor. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Role of the Vacuolar-ATPase in Sindbis Virus Infection

JOURNAL OF VIROLOGY, 85(3), 1257–1266.

By: S. Hunt n, R. Hernandez n & D. Brown n

MeSH headings : Animals; Cells, Cultured; Cricetinae; Enzyme Inhibitors / metabolism; Host-Pathogen Interactions; Macrolides / metabolism; Sindbis Virus / physiology; Vacuolar Proton-Translocating ATPases / metabolism; Virus Internalization; Virus Replication
TL;DR: The results suggest that in BHK cells in the presence of bafilomycin A1, virus RNA enters the cell and is translated, but replication and proper folding of the product proteins requires the function of the V-ATPase. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype

VIROLOGY JOURNAL, 8.

By: K. Smith*, K. Nanda*, C. Spears*, M. Ribeiro n, R. Vancini n, A. Piper n, G. Thomas*, M. Thomas*, D. Brown n, R. Hernandez n

MeSH headings : Aedes; Amino Acid Sequence; Animals; Antibodies, Neutralizing / blood; Antibodies, Viral / blood; Cell Line; Dengue Virus / genetics; Dengue Virus / immunology; Dengue Virus / pathogenicity; Dengue Virus / physiology; Host Specificity; Mice; Mice, Inbred BALB C; Models, Molecular; Molecular Sequence Data; Mutant Proteins / genetics; Mutant Proteins / immunology; Mutant Proteins / metabolism; Sequence Deletion; Viral Envelope Proteins / genetics; Viral Envelope Proteins / immunology; Viral Envelope Proteins / metabolism
TL;DR: Novel host-range mutants of DV2 were created that have preferential growth in insect cells and impaired infectivity in mammalian cells and may be applied to many other insect-borne viral diseases for which no current effective therapies exist. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Growth and Maintenance of Baby Hamster Kidney (BHK) Cells

Current Protocols in Microbiology, 17(1), A.4H.1-A.4H.7.

By: R. Hernandez n & D. Brown n

MeSH headings : Animals; Cell Culture Techniques; Cell Line; Cricetinae; Culture Media / chemistry; Fibroblasts; Kidney / cytology; Mesocricetus; Sindbis Virus / growth & development; Virus Cultivation / methods
TL;DR: The BHK21 cell line was established in 1961 from the kidneys of 5 Syrian hamsters from litter number 21 and has been a laboratory standard for the growth of countless viruses and the study of many biological processes. (via Semantic Scholar)
Source: Crossref
Added: August 14, 2021

2010 journal article

Growth and Maintenance of Chick Embryo Fibroblasts (CEF)

Current Protocols in Microbiology, 17(1), A.4I.1-A.4I.8.

By: R. Hernandez n & D. Brown n

MeSH headings : Animals; Cell Culture Techniques; Cells, Cultured; Chick Embryo; Culture Media / chemistry; Fibroblasts
TL;DR: Primary cultures of chick embryo fibroblasts are widely used for the cultivation of viruses and Protocols for the growth and maintenance of CEF cells in the laboratory are provided. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Crossref
Added: August 16, 2021

2010 journal article

Growth and Maintenance of Mosquito Cell Lines

Current Protocols in Microbiology, 17(1), A.4J.1-A.4J.8.

By: R. Hernandez n & D. Brown n

MeSH headings : Animals; Cell Culture Techniques / methods; Cell Line; Culicidae; Culture Media / chemistry
TL;DR: Mosquito cells (Aedes albopictus) are among the most common insect cells emerging as new sources of cell cultures to use in basic research and in the pharmaceutical industry. (via Semantic Scholar)
Source: Crossref
Added: October 9, 2021

2010 journal article

Insect response to alphavirus infection-Establishment of alphavirus persistence in insect cells involves inhibition of viral polyprotein cleavage

VIRUS RESEARCH, 150(1-2), 73–84.

By: U. Mudiganti n, R. Hernandez n & D. Brown n

author keywords: Arbovirus; Sindbis; Insect cells; Microarray; Persistent infection
MeSH headings : Alphavirus / pathogenicity; Alphavirus / physiology; Animals; Cell Line; Drosophila; Polyproteins / metabolism; Viral Proteins / metabolism; Virus Replication
TL;DR: It is proposed that inefficient cleavage of the polyprotein may lead to reduced levels of virus seen as persistence is established, and the up-regulation of a wide-spectrum protease inhibitor, Thiol-ester containing Protein (TEP) II is confirmed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2010 journal article

The Structure of Sindbis Virus Produced from Vertebrate and Invertebrate Hosts as Determined by Small-Angle Neutron Scattering

JOURNAL OF VIROLOGY, 84(10), 5270–5276.

MeSH headings : Animals; Cell Line; Cricetinae; Culicidae; Scattering, Small Angle; Sindbis Virus / chemistry; Sindbis Virus / growth & development; Virion / chemistry; Virion / isolation & purification
TL;DR: The first study of the structural differences between Sindbis viruses grown in mammalian and insect cells are reported, which were determined by small-angle neutron scattering (SANS), a nondestructive technique that did not decrease the infectivity of the Sindbis virus particles studied. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2009 journal article

A high capacity Alphavirus heterologous gene delivery system

VIROLOGY, 390(2), 368–373.

By: K. Nanda n, R. Vancini n, M. Ribeiro n, D. Brown n & R. Hernandez n

author keywords: Alphavirus; Gene delivery; Morphological variants
MeSH headings : Animals; Cell Line; Cricetinae; Culicidae; Gene Transfer Techniques; Genetic Vectors; Microscopy, Electron, Transmission; Mutation, Missense; Sindbis Virus / genetics; Viral Proteins / genetics; Virion / ultrastructure
TL;DR: The first generation prototype larger vehicle, described here, carries a approximately 18 kb cDNA insert, however it is conceivable that RNA as large as 32 kb could be transcribed and packaged, and the resulting cargo containing virus particles are infectious. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Differential Incorporation of Cholesterol by Sindbis Virus Grown in Mammalian or Insect Cells

JOURNAL OF VIROLOGY, 83(18), 9113–9121.

By: A. Hafer n, R. Whittlesey n, D. Brown n & R. Hernandez n

MeSH headings : Alphavirus Infections / etiology; Animals; Cell Line; Cell Membrane / chemistry; Cholesterol / analysis; Cholesterol / deficiency; Cholesterol / metabolism; Insecta; Lipids / analysis; Mammals; Mass Spectrometry; Sindbis Virus / metabolism; Sindbis Virus / pathogenicity
TL;DR: The cholesterol dependence reported for infectivity and assembly of Sindbis virus is a reflection of differences in the insect cell lines used and the methods of delipidation, and the amount of cholesterol in the host cell membrane in and of itself has no effect on the process of virus assembly or on the ability of virus to infect cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
12. Responsible Consumption and Production (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Helical virus particles formed from morphological subunits of a membrane containing icosahedral virus

VIROLOGY, 385(2), 285–293.

By: J. Kononchik n, S. Nelson*, R. Hernandez n & D. Brown n

author keywords: Sindbis virus; Virus assembly; Protein interactions; Morphological variants; Helix symmetry; Icosahedral symmetry
MeSH headings : Animals; Cricetinae; Furin / metabolism; Membrane Glycoproteins / genetics; Membrane Glycoproteins / physiology; Microscopy, Electron, Scanning; Mutation; Sindbis Virus / chemistry; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Proteins / metabolism; Virion / chemistry; Virus Assembly
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Construction and characterization of mutant Dengue2 virus vaccine candidates displaying a host-range phenotype

Vaccine.

By: K. Smith, K. Nanda, C. Slominski, R. Hernandez, D. Brown & M. Thomas

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Sindbis virus conformational changes induced by a neutralizing anti-E1 monoclonal antibody

JOURNAL OF VIROLOGY, 82(12), 5750–5760.

By: R. Hernandez n, A. Paredes* & D. Brown n

MeSH headings : Animals; Antibodies, Monoclonal / pharmacology; Cricetinae; Epitopes; Hydrogen-Ion Concentration; Immunoglobulin Fab Fragments / immunology; Kidney / cytology; Membrane Glycoproteins / immunology; Neutralization Tests; Peptide Mapping; Protein Binding; Protein Conformation; Sindbis Virus / chemistry; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Envelope Proteins / immunology; Virion / physiology; Virion / ultrastructure
TL;DR: A model is proposed which postulates that the induction of conformational changes in the infectious state of a metastable enveloped virus may be a general mechanism of antibody inactivation of virus infectivity. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Infection of cells by Sindbis virus at low temperature

Virology, 362(2), 461–467.

By: G. Wang n, R. Hernandez n, keith & D. Brown n

MeSH headings : Animals; Cell Fusion; Cell Line; Cricetinae; Endocytosis; Microscopy, Immunoelectron; Neutral Red / metabolism; Sindbis Virus / physiology; Temperature; Time Factors; Viral Plaque Assay; Virus Internalization
TL;DR: The ability of Sind Bis virus to infect Baby Hamster Kidney cells at temperatures which block endocytosis is examined and it is found that under these conditions Sindbis virus infects cells in a temperature- and time-dependent fashion. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins

JOURNAL OF VIROLOGY, 81(12), 6231–6240.

MeSH headings : Amino Acid Sequence; Animals; Capsid / chemistry; Cell Line; Chromatography, Liquid; Cricetinae; Cysteine / chemistry; Disulfides / chemistry; Iodoacetamide / pharmacology; Mass Spectrometry; Membrane Glycoproteins / chemistry; Molecular Sequence Data; Mutation; Protein Conformation; Viral Envelope Proteins / chemistry
TL;DR: Native virus particles alkylated with iodoacetamide demonstrated a 4-log decrease in viral infectivity, which suggests that the modification of free cysteinyl residues results in the loss of infectivity by destabilizing the virus particle or that a rearrangement of disulfide bonds, which is required forinfectivity, is blocked by the modification. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

A colorimetric assay for viral agents that produce cytopathic effects

Journal of Virological Methods, 135(1), 56–65.

Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

A colorimetric assay for viral agents that produce cytopathic effects

Journal of Virological Methods, 135(1), 56–65.

By: C. Heldt n, R. Hernandez n, U. Mudiganti n, P. Gurgel, D. Brown n & R. Carbonell n

author keywords: MTT; TCID50; plaque assay; parvovirus; Sindbis virus
MeSH headings : Animals; Cell Line; Colorimetry / methods; Cricetinae; Cytopathogenic Effect, Viral; Dogs; Formazans / metabolism; Humans; Minute Virus of Mice / pathogenicity; Parvovirus, Canine / pathogenicity; Parvovirus, Porcine / pathogenicity; Reproducibility of Results; Sindbis Virus / pathogenicity; Swine; Tetrazolium Salts / metabolism; Viral Plaque Assay; Viruses / pathogenicity
TL;DR: MTT has been applied to measure cytopathic effect produced by viral infection for different members of the parvovirus family and an unrelated virus, Sindbis, which is routinely titrated by plaque assay. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: August 28, 2020

2006 journal article

Mutations in the endodomain of Sindbis virus glycoprotein E2 define sequences critical for virus assembly

JOURNAL OF VIROLOGY, 80(9), 4458–4468.

By: J. West n, R. Hernandez n, D. Ferreira n & D. Brown n

MeSH headings : Amino Acid Sequence; Animals; Capsid Proteins / genetics; Capsid Proteins / metabolism; Cell Line; Cricetinae; Gene Deletion; Microscopy, Electron, Transmission; Molecular Sequence Data; Mutation / genetics; Protein Structure, Tertiary; Sequence Alignment; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Tyrosine / genetics; Tyrosine / metabolism; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Viral Envelope Proteins / metabolism; Virus Assembly
TL;DR: Submo acid substitutions and deletions in a region of the E2 tail (E2 amino acids 408 to 415) that is initially integrated into the endoplasmic reticulum are produced to define protein interactions critical for virus assembly and suggest a fundamental difference between Sindbis virus assembly in mammalian and insect cells. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Role of a conserved tripeptide in the endodomain of Sindbis virus glycoprotein E2 in virus assembly and function

JOURNAL OF GENERAL VIROLOGY, 87, 657–664.

By: J. West n & D. Brown n

MeSH headings : Acids; Amino Acid Sequence; Animals; Cell Fusion; Cell Line; Cricetinae; Molecular Sequence Data; Protein Structure, Tertiary / genetics; Protein Structure, Tertiary / physiology; Sindbis Virus / pathogenicity; Sindbis Virus / physiology; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Viral Envelope Proteins / physiology; Virulence; Virus Assembly
TL;DR: The loss of infectivity in the double mutant is accompanied by the loss of the ability to fuse cells after brief exposure to acid pH, which supports the idea that these residues are vital for production of infectious/functional virus; however, they are dispensable for assembly. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Sindbis virus infection of two model insect cell systems - A comparative study

Virus Research, 122(1-2), 28–34.

By: U. Mudiganti n, R. Hernandez n, D. Ferreira n & D. Brown n

MeSH headings : Animals; Cell Line; Cell Shape; Cell Survival; Culicidae; Cytopathogenic Effect, Viral; Drosophila; Microscopy, Electron, Transmission; Sindbis Virus / growth & development; Viral Plaque Assay
TL;DR: A comparative study of Sindbis virus infection of Schnieder-2 Drosophila (S2) cells to cells of the mosquito Aedes albopictus (clone U4.4) was undertaken, establishing S2 cells as an additional invertebrate model system to study the interactions of an invertebrates host with Sindbisirus. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2006 journal article

Single and multiple deletions in the transmembrane domain of the Sindbis virus E2 glycoprotein identify a region critical for normal virus growth

VIROLOGY, 347(1), 199–207.

author keywords: Sindis virus; E2 glycoprotein; transmembrane domain; virus assembly; compensatory mutations
MeSH headings : Amino Acid Sequence; Animals; Cell Line; Cricetinae; Models, Molecular; Molecular Sequence Data; Protein Structure, Tertiary; Sequence Deletion; Sindbis Virus / genetics; Sindbis Virus / growth & development; Sindbis Virus / physiology; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Virus Replication / genetics
TL;DR: Results suggest that the position of the deletion and the length of the C terminal region of the E2 transmembrane domain is vital for normal virus production, and deletion mutants resulting in decreased infectivity produce particles that appear to be processed and transported correctly suggesting a role involved in virus entry. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Structural characterization of the E2 glycoprotein from Sindbis by lysine biotinylation and LC-MS/MS

VIROLOGY, 348(1), 216–223.

By: J. Sharp, S. Nelson, D. Brown* & K. Tomer

author keywords: Sindbis; mass spectrometry; surface labeling; Alphavirus; glycoprotein
MeSH headings : Amino Acid Sequence; Animals; Biotin / metabolism; Cell Line; Cricetinae; Lysine / metabolism; Mass Spectrometry / methods; Microscopy, Electron, Transmission; Molecular Sequence Data; Protein Structure, Quaternary; Sindbis Virus / chemistry; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Staining and Labeling / methods; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / ultrastructure; Viral Plaque Assay; Virion / chemistry; Virion / ultrastructure
TL;DR: The organization of the E2 glycoprotein was probed by surface biotinylation of intact virions and it was confirmed that the E1 protein is almost completely buried in the virus structure. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

In vivo processing and isolation of furin protease-sensitive alphavirus glycoproteins: a new technique for producing mutations in virus assembly

VIROLOGY, 332(2), 629–639.

By: S. Nelson n, R. Hernandez n, D. Ferreira n & D. Brown n

author keywords: alphavirus; Sindbis; membrane glycoproteins; form protease; virus assembly
MeSH headings : Animals; Capsid Proteins / genetics; Cell Line; Cricetinae; Dithiothreitol / pharmacology; Ethylmaleimide / pharmacology; Furin / pharmacology; Kidney; Mutagenesis, Site-Directed; Mutagens; Sindbis Virus / drug effects; Sindbis Virus / genetics; Sindbis Virus / isolation & purification; Viral Envelope Proteins / genetics; Viral Structural Proteins / genetics
TL;DR: In a new approach to the production of membrane-free membrane glycoproteins, furin protease recognition motifs were installed at various positions in the E1 glycoprotein ectodomain to provide a unique method for studying the mechanism of virus assembly and protein structure without altering crucial early events in protein assembly, folding, and maturation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Single amino acid insertions at the junction of the Sindbis virus e2 transmembrane domain and endodomain disrupt virus envelopment and alter infectivity

Journal of Virology, 79(12), 7682–7697.

By: R. Hernandez, D. Ferreira, C. Sinodis, K. Litton & D. Brown

Source: NC State University Libraries
Added: August 6, 2018

2005 chapter

Trust-Based Secure Workflow Path Construction

In B. Benatallah, F. Casati, & P. Traverso (Eds.), Service-Oriented Computing – ICSOC 2007 (pp. 382–395).

By: M. Altunay n, D. Brown n, G. Byrd n & R. Dean n

Contributors: M. Altunay n, D. Brown n, G. Byrd n & R. Dean n

Ed(s): B. Benatallah, F. Casati & P. Traverso

TL;DR: A workflow management framework that fully integrates trust and security into the workflow planning logic, which considers not only trust relationships between the workflow requestor and individual services, but also trust relationships among the services themselves. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 6, 2018

2004 journal article

Conformational changes in Sindbis virions resulting from exposure to low pH and interactions with cells suggest that cell penetration may occur at the cell surface in the absence of membrane fusion

Virology, 324(2), 373–386.

By: A. Paredes, D. Ferreira, M. Horton, A. Saad, H. Tsuruta, R. Johnston, W. Klimstra, K. Ryman ...

Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

Conformational changes in Sindbis virions resulting from exposure to low pH and interactions with cells suggest that cell penetration may occur at the cell surface in the absence of membrane fusion

Virology, 324(2), 373–386.

By: A. Paredes*, D. Ferreira n, M. Horton n, A. Saad*, H. Tsuruta*, R. Johnston*, W. Klimstra*, K. Ryman* ...

author keywords: Sindbis virus; electron cryo-microscopy; pH conditions; structure; infection; membrane fusion
MeSH headings : Animals; Cell Fusion; Cell Line; Cricetinae; Cryoelectron Microscopy; Hydrogen-Ion Concentration; Membrane Fusion; Microscopy, Electron; Protein Conformation; Sindbis Virus / chemistry; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / ultrastructure; Virion / chemistry; Virion / physiology; Virus Replication
TL;DR: Investigation of the requirements for the induction of virus-mediated, low pH-induced cell-cell fusion and cell-virus fusion found that exposure to acid pH was required to establish conditions for membrane fusion but that membrane fusion did not occur until return to neutral pH. (via Semantic Scholar)
Source: Crossref
Added: October 9, 2021

2004 journal article

Rapid preparative purification of West Nile and Sindbis virus PCR products utilizing a microbore anion-exchange column

Journal of Virological Methods, 120(2), 141–149.

By: R. Hernandez, S. Nelson, J. Salm, D. Brown & A. Alpert

Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

Rapid preparative purification of West Nile and Sindbis virus PCR products utilizing a microbore anion-exchange column

Journal of Virological Methods, 120(2), 141–149.

By: R. Hernandez n, S. Nelson n, J. Salm n, D. Brown n & A. Alpert

author keywords: Sindbis virus; West Nile virus; PCR products; preparative anion-exchange HPLC purification
MeSH headings : Anions / analysis; Anions / isolation & purification; Chromatography, High Pressure Liquid; DNA, Viral / analysis; DNA, Viral / isolation & purification; Polymerase Chain Reaction / methods; Sindbis Virus / genetics; Sindbis Virus / isolation & purification; Time Factors; Virology / methods; West Nile virus / genetics; West Nile virus / isolation & purification
TL;DR: This paper describes a simple and relatively inexpensive micro-preparative HPLC method to purify and analyze nM quantities of DNA that differs from existing methodologies because separation is based on size and charge as well as the overall G + C content of the PCR product. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Crossref
Added: October 9, 2021

2003 journal article

Deletions in the transmembrane domain of a Sindbis virus glycoprotein alter virus infectivity, stability, and host range

JOURNAL OF VIROLOGY, 77(23), 12710–12719.

By: R. Hernandez n, C. Sinodis n, M. Horton n, D. Ferreira n, C. Yang n & D. Brown n

MeSH headings : Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cricetinae; DNA Primers; Lipid Bilayers; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Sequence Deletion; Sequence Homology, Amino Acid; Sindbis Virus / genetics; Sindbis Virus / physiology; Virus Assembly
TL;DR: It is found that progressive truncations in the transmembrane domain profoundly affected production of infectious virus in a cyclic fashion and also found that membrane composition effects protein-protein and protein- Membrane interactions during virus assembly. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Morphological variants of Sindbis virus produced by a mutation in the capsid protein

VIROLOGY, 307(1), 54–66.

By: D. Ferreira n, R. Hernandez n, M. Horton n & D. Brown n

MeSH headings : Animals; Capsid Proteins / genetics; Cell Line; Cricetinae; Kidney; Microscopy, Electron; Mutation; RNA, Viral / genetics; Sindbis Virus / genetics; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Transcription, Genetic; Transfection; Viral Plaque Assay
TL;DR: The data presented in the following manuscript show that mutations in the capsid protein can result in the assembly of the virus structural proteins into icosahedra of different triangulation numbers, and support the hypothesis that families of icosadeltahedra would be developed by altering the distance between the points of insertion of the five-fold axis. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Exposure to low pH is not required for penetration of mosquito cells by Sindbis virus

JOURNAL OF VIROLOGY, 75(4), 2010–2013.

By: R. Hernandez n, T. Luo n & D. Brown n

MeSH headings : Aedes / virology; Ammonium Chloride / pharmacology; Animals; Cells, Cultured; Chloroquine / pharmacology; Hydrogen-Ion Concentration; Sindbis Virus / drug effects; Sindbis Virus / physiology; Virus Replication / drug effects
TL;DR: The results suggest that while these two drugs raise the pH of endosomes, they do not block insect cell penetration, and support previous results suggesting that exposure to an acid environment within the cell may not be an obligatory step in the process of infection of cells by alphaviruses. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

FTIR and resonance Raman studies of nitric oxide binding to H93G cavity mutants of myoglobin

BIOCHEMISTRY, 40(49), 15047–15056.

By: M. Thomas n, D. Brown n, S. Franzen n & S. Boxer n

MeSH headings : Animals; Binding Sites; Enzyme Inhibitors / metabolism; Free Radical Scavengers / metabolism; Imidazoles / metabolism; Molecular Structure; Mutation; Myoglobin / genetics; Myoglobin / metabolism; Nitric Oxide / metabolism; Protein Binding; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman
TL;DR: The data presented here do not appear to support the presence of pi-back-bonding or an inverse trans effect of NO binding in Mb mutants that alter the electrostatic environment of the heme pocket, leading to the observation that nu(N-O) and nu(Fe-NO) are not quantitatively correlated for the MbNO complexes presented here. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2001 journal article

Locations of carbohydrate sites on alphavirus glycoproteins show that E1 forms an icosahedral scaffold

CELL, 105(1), 127–136.

By: S. Pletnev*, W. Zhang*, S. Mukhopadhyay*, B. Fisher*, R. Hernandez n, D. Brown n, T. Baker*, M. Rossmann*, R. Kuhn*

MeSH headings : Alphavirus / chemistry; Alphavirus / genetics; Alphavirus / ultrastructure; Amino Acid Motifs / genetics; Animals; Binding Sites / genetics; Carbohydrates / chemistry; Cell Line; Cricetinae; Cryoelectron Microscopy; Encephalitis Viruses, Tick-Borne / chemistry; Glycosylation; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / genetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Ross River virus / chemistry; Sindbis Virus / chemistry; Sindbis Virus / ultrastructure; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Viral Fusion Proteins / chemistry; Viral Fusion Proteins / genetics
TL;DR: There are 80 spikes on the surface of Sindbis virus arranged as an icosahedral surface lattice, consistent with the observed structural changes that occur on fusion with host cell membranes, suggesting a similar fusion mechanism for alpha- and flaviviruses. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

A single deletion in the membrane-proximal region of the Sindbis virus glycoprotein E2 endodomain blocks virus assembly

JOURNAL OF VIROLOGY, 74(9), 4220–4228.

By: R. Hernandez n, H. Lee*, C. Nelson n & D. Brown n

MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Biological Transport; Cell Line; Cell Membrane / metabolism; Cricetinae; Cytoplasm / metabolism; Humans; Intracellular Fluid / metabolism; Molecular Sequence Data; Mutagenesis; Nucleocapsid / metabolism; Sindbis Virus / genetics; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Viral Envelope Proteins / genetics; Viral Envelope Proteins / metabolism; Viral Envelope Proteins / physiology; Viral Structural Proteins / metabolism; Virus Assembly / physiology
TL;DR: A deletion mutant is constructed, ΔK391, in which a nonconserved lysine at the membrane-cytoplasm junction of the E2 tail has been deleted and it is proposed that the phenotypic differences displayed by this mutant in the two diverse host cells arise from fundamental differences in the lipid composition of the insect cell membranes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Sindbis virus glycoprotein E1 is divided into two discrete domains at amino acid 129 by disulfide bridge connections

JOURNAL OF VIROLOGY, 74(19), 9313–9316.

By: B. Phinney n & D. Brown n

MeSH headings : Amino Acid Sequence; Membrane Glycoproteins / chemistry; Molecular Sequence Data; Sindbis Virus / chemistry; Sulfides; Viral Envelope Proteins / chemistry
TL;DR: The E1 membrane glycoprotein of Sindbis virus contains structural and functional domains, which are conformationally dependent on the presence of intramolecular disulfide bridges, which divide the protein into two domains at amino acid 129. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

The surface conformation of sindbis virus glycoproteins E1 and E2 at neutral and low pH, as determined by mass spectrometry-based mapping

JOURNAL OF VIROLOGY, 74(12), 5667–5678.

By: B. Phinney n, K. Blackburn* & D. Brown n

MeSH headings : Capsid / chemistry; Capsid / metabolism; Disulfides / metabolism; Hydrogen-Ion Concentration; Iodine Radioisotopes / metabolism; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / metabolism; Pepsin A / metabolism; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Protein Binding; Protein Structure, Tertiary; Serine Endopeptidases / metabolism; Silver Staining; Sindbis Virus; Software; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Surface Properties; Trypsin / metabolism; Tyrosine / metabolism; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / metabolism
TL;DR: The techniques of limited proteolysis and amino acid chemical modification in combination with mass spectrometry improve the understanding of the structure of the virus membrane and have implications for understanding the protein conformational changes which accompany the process of virus-cell membrane fusion. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Comparison of Sindbis virus-induced pathology in mosquito and vertebrate cell cultures

VIROLOGY, 240(2), 193–201.

By: A. Karpf* & D. Brown*

MeSH headings : Animals; Apoptosis / genetics; Cell Culture Techniques; Cell Cycle; Cricetinae; Culicidae / cytology; Culicidae / virology; Cytopathogenic Effect, Viral; Flow Cytometry; Sindbis Virus / pathogenicity; Sindbis Virus / ultrastructure
TL;DR: The data suggest that the pathology induced in mosquito cell cultures by Sindbis virus infection may be distinct from the pathology which appears in vertebrate cell cultures. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Laser assisted soldering: Microdroplet accumulation with a microjet device

Lasers in Surgery and Medicine, 23(4), 213–220.

By: E. Chan*, Q. Lu*, B. Bell*, M. Motamedi*, C. Frederickson*, D. Brown n, I. Kovach*, A. Welch*

TL;DR: This work investigated the feasibility of a microjet to dispense protein solder for laser assisted soldering and found it to be feasible. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with sindbis virus

Journal of Virology, 71(9), 7119–7123.

By: A. Karpf, E. Lenches, E. Strauss, J. Strauss & D. Brown

Source: NC State University Libraries
Added: August 6, 2018

1997 journal article

The formation of intramolecular disulfide bridges is required for induction of the Sindbis virus mutant TS23 phenotype

Journal of Virology, 71(10), 7696–7703.

By: M. Carleton & D. Brown

Source: NC State University Libraries
Added: August 6, 2018

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