Daphne Collias

College of Engineering

Works (4)

Updated: July 13th, 2023 21:15

2023 journal article

Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria

NATURE COMMUNICATIONS, 14(1).

By: D. Collias*, E. Vialetto *, J. Yu *, K. Co*, E. Almasi *, A. Ruettiger, T. Achmedov*, T. Strowig *, C. Beisel n

TL;DR: It is shown that systematically attenuating DNA targeting activity enables RecA-mediated repair in different bacteria, allowing chromosomal cleavage to drive genome editing. (via Semantic Scholar)
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Added: July 3, 2023

2021 review

CRISPR technologies and the search for the PAM-free nuclease

[Review of ]. NATURE COMMUNICATIONS, 12(1).

By: D. Collias n & C. Beisel n

MeSH headings : Binding Sites / genetics; CRISPR-Associated Protein 9 / classification; CRISPR-Associated Protein 9 / genetics; CRISPR-Associated Protein 9 / metabolism; CRISPR-Cas Systems; Gene Editing / methods; Models, Genetic; Nucleotide Motifs; Phylogeny; Protein Engineering / methods; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism
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Added: February 22, 2021

2020 journal article

A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

SCIENCE ADVANCES, 6(29).

By: D. Collias n, R. Leenay n, R. Slotkowski n, Z. Zuo *, S. Collins n, B. McGirr n, J. Liu *, C. Beisel n

TL;DR: It is reported that SpyCas9 also recognizes sequences within an N(A/C/T)GG motif, which could have implications for precise editing, off-target predictions, and CRISPR-based immunity. (via Semantic Scholar)
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Added: August 10, 2020

2019 journal article

An educational module to explore CRISPR technologies with a cell-free transcription-translation system

SYNTHETIC BIOLOGY, 4(1).

By: D. Collias n, R. Marshall*, S. Collins n, C. Beisel* & V. Noireaux *

author keywords: CRISPR; Cas9; education modules; synthetic biology; TXTL
TL;DR: Three educational modules intended to expose undergraduate students to CRISPR technologies using TXTL are described and are expected to allow students to experience the power and promise of CRISpr technologies in the classroom and to engage with their instructor and peers about the opportunities and potential risks for society. (via Semantic Scholar)
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Added: February 27, 2020

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