Works (21)

Updated: April 13th, 2024 05:01

2024 journal article

Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant

JOURNAL OF CHROMATOGRAPHY A, 1718.

By: D. Pham*, M. Linova*, W. Smith n, H. Brown n, D. Elhanafi n, J. Fan n, J. Lavoie n, J. Woodley*, R. Carbonell n

author keywords: Multimodal ligand; Membrane chromatography; Nonwoven membranes; Single-chain variable fragment (scFv); Pichia pastoris fermentation
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: April 8, 2024

2023 journal article

Draft Genome Sequences of 158 Listeria monocytogenes Strains Isolated from Black Bears (Ursus americanus) in the United States

Microbiology Resource Announcements, 12(7).

By: P. Brown n, Y. Chen*, M. Ivanova*, P. Leekitcharoenphon*, C. Parsons n, J. Niedermeyer n, N. Gould n, J. Strules n ...

Ed(s): D. Rasko

TL;DR: The draft genome sequences of 158 L. monocytogenes strains isolated from black bears (Ursus americanus) in the southeastern United States between 2014 and 2017 are reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID, Crossref
Added: June 19, 2023

2023 article

Draft Genome Sequences of Closely Related Listeria monocytogenes Lineage III Strains Isolated from a Food Processing Environment and a Case of Human Listeriosis

Brown, P., Kanenaka, R., Chen, Y., Ivanova, M., Leekitcharoenphon, P., Elhanafi, D., & Kathariou, S. (2023, May 22). (D. Rasko, Ed.). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 5.

By: P. Brown n, R. Kanenaka*, Y. Chen*, M. Ivanova*, P. Leekitcharoenphon*, D. Elhanafi n, S. Kathariou n

Ed(s): D. Rasko

TL;DR: The genome sequences of three closely related lineage III strains from Hawaii are reported, namely, one isolated from a human case and twoisolated from a produce storage facility. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID
Added: June 12, 2023

2023 journal article

Draft genome sequences of a historical collection of Listeria monocytogenes from humans and other sources, 1926-1964

MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 9.

By: P. Brown n, R. Murray*, S. Galsworthy*, M. Ivanova*, P. Leekitcharoenphon*, T. Ward*, Z. Kucerova*, Y. Chen* ...

Ed(s): J. Dennehy

author keywords: Listeria monocytogenes; food-borne pathogens; listeriosis; DNA sequencing; genomes; intracellular pathogens; historical strains
TL;DR: It is shown that listeria monocytogenes can persistently contaminate food processing environments and tolerate sanitizers, and a diverse panel of 83 strains from 1926 to 1964 are reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID
Added: October 16, 2023

2023 journal article

Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by Escherichia coli and Staphylococcus aureus Infection

ANIMALS, 13(5).

By: A. Sadat*, A. Farag*, D. Elhanafi n, A. Awad*, E. Elmahallawy*, N. Alsowayeh*, M. El-khadragy*, G. Elshopakey*

author keywords: mastitis; E; coli; S; aureus; oxidative; antioxidant molecules; APP; inflammatory cytokines
TL;DR: The study aimed to investigate the mastitis’ emerging causative agents and their antimicrobial sensitivity, in addition to the hematological, biochemical indicators, oxidative biomarkers, acute phase protein (APP), and inflammatory cytokine changes in dairy farms in Gamasa, Dakahlia Governorate, Egypt. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: April 4, 2023

2023 journal article

Investigation of a Listeria monocytogenes Chromosomal Immigration Control Region Reveals Diverse Restriction Modification Systems with Complete Sequence Type Conservation

MICROORGANISMS, 11(3).

By: P. Brown n, S. Lee*, D. Elhanafi n, W. Tham*, M. Danielsson-Tham*, G. Lopez-Valladares*, Y. Chen*, M. Ivanova*, P. Leekitcharoenphon*, S. Kathariou n

author keywords: Listeria; restriction modification system; immigration control region; whole genome sequencing; chromosomal hotspot
TL;DR: Analysis of L. monocytogenes genomes indicates that the ICR has a high propensity for RM systems which are intraclonaly conserved and may impact bacteriophage susceptibility as well as ST emergence and stability. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: March 10, 2023

2023 journal article

Pseudo-affinity capture of <i>K. phaffii</i> host cell proteins in flow-through mode: Purification of protein therapeutics and proteomic study

SEPARATION AND PURIFICATION TECHNOLOGY, 326.

By: S. Sripada n, D. Elhanafi*, L. Collins n, T. Williams n, M. Linova*, J. Woodley*, C. Boi, S. Menegatti n

Contributors: S. Sripada n, D. Elhanafi*, L. Collins n, T. Williams n, M. Linova*, J. Woodley*, C. Boi, S. Menegatti n

author keywords: Komagataella phaffii; Chromatography; Protein purification; Monoclonal antibodies; Antibody fragment; Proteomics
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: October 23, 2023

2022 journal article

Development of peptide ligands for the purification of a-1 antitrypsin from cell culture fluids

JOURNAL OF CHROMATOGRAPHY A, 1679.

By: W. Chu n, R. Prodromou n, B. Moore n, D. Elhanafi n, R. Kilgore n, S. Shastry n, S. Menegatti n

author keywords: a-1 antitrypsin; Peptide ligands; Affinity chromatography; Protein purification; CHO cell culture harvest
MeSH headings : Animals; CHO Cells; Cell Culture Techniques; Chromatography, Affinity; Cricetinae; Cricetulus; Ligands; Molecular Docking Simulation; Peptides; alpha 1-Antitrypsin
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: October 11, 2022

2021 article

Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes

LISTERIA MONOCYTOGENES, 2 EDITION, Vol. 2220, pp. 177–185.

By: C. Parsons n, R. Azizoglu n, D. Elhanafi n & S. Kathariou n

author keywords: Transposon; Mutant; Listeria monocytogenes; Complementation
MeSH headings : Electroporation / methods; Genetic Vectors / genetics; Humans; Listeria monocytogenes / genetics; Listeriosis / microbiology; Mutagenesis, Insertional / methods; Mutation; Plasmids / genetics; Polymerase Chain Reaction / methods; Retroelements
TL;DR: This chapter describes the construction and screening of mutant libraries of L. monocytogenes using the plasmid pMC38, carrying a mariner-based transposon system (TC1/mariner) and constructed by Cao et al. (via Semantic Scholar)
Source: Web Of Science
Added: August 30, 2021

2014 journal article

Genetic Characterization of Plasmid-Associated Triphenylmethane Reductase in Listeria monocytogenes

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(17), 5379–5385.

By: V. Dutta n, D. Elhanafi n, J. Osborne n, M. Martinez n & S. Kathariou n

MeSH headings : Benzalkonium Compounds / metabolism; Benzalkonium Compounds / toxicity; Biotransformation; DNA Transposable Elements; Gene Order; Gentian Violet / metabolism; Gentian Violet / toxicity; Listeria monocytogenes / enzymology; Listeria monocytogenes / genetics; Listeria monocytogenes / isolation & purification; Oxidation-Reduction; Oxidoreductases / genetics; Oxidoreductases / metabolism; Plasmids; Rosaniline Dyes / metabolism; Rosaniline Dyes / toxicity; Trityl Compounds / metabolism
TL;DR: A potential role of TMR as a previously unknown adaptive attribute for environmental persistence of L. monocytogenes is suggested. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Conservation and Distribution of the Benzalkonium Chloride Resistance Cassette bcrABC in Listeria monocytogenes

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(19), 6067–6074.

By: V. Dutta n, D. Elhanafi n & S. Kathariou n

MeSH headings : Benzalkonium Compounds / pharmacology; Chromosomes, Bacterial; Conserved Sequence; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Disinfectants / pharmacology; Drug Resistance, Bacterial; Gene Transfer, Horizontal; Listeria monocytogenes / drug effects; Listeria monocytogenes / genetics; Molecular Sequence Data; Multigene Family; Plasmids; Sequence Analysis, DNA; Transcription, Genetic
TL;DR: Analysis of a panel of 116 Listeria monocytogenes strains of diverse serotypes and sources revealed that all but one of the 71 benzalkonium chloride-resistant (BCr) isolates harbored bcrABC, previously identified on a large plasmid of the 1998-1999 hot dog outbreak strain H7858. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(21), 7549–7556.

By: S. Katharios-Lanwermeyer*, M. Rakic-Martinez*, D. Elhanafi n, S. Ratani*, J. Tiedje* & S. Kathariou*

MeSH headings : Anti-Infective Agents, Local / pharmacology; Bacterial Proteins / genetics; Benzalkonium Compounds / pharmacology; Cadmium / pharmacology; Conjugation, Genetic; Drug Resistance, Bacterial / genetics; Food Handling; Gene Transfer, Horizontal; Listeria / drug effects; Listeria / genetics; Listeria / pathogenicity; Temperature
TL;DR: The results suggest that nonpathogenic Listeria spp. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Conservation of Genomic Localization and Sequence Content of Sau3AI-Like Restriction-Modification Gene Cassettes among Listeria monocytogenes Epidemic Clone I and Selected Strains of Serotype 1/2a

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(16), 5577–5584.

By: S. Yildirim n, D. Elhanafi n, W. Lin*, A. Hitchins*, R. Siletzky n & S. Kathariou n

MeSH headings : Bacterial Typing Techniques; Cluster Analysis; Conserved Sequence; DNA Fingerprinting; DNA Modification Methylases / genetics; DNA Modification Methylases / metabolism; DNA Restriction-Modification Enzymes / genetics; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Deoxyribonucleases, Type II Site-Specific / genetics; Electrophoresis, Gel, Pulsed-Field; Gene Order; Genes, Bacterial; Genotype; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Molecular Sequence Data; Recombinases / genetics; Sequence Analysis, DNA; Sequence Deletion; Serotyping
TL;DR: Deletion of the RM cassette in the ECI strain F2365 and the serotype 1/2a strain A7 rendered the DNA of both strains susceptible to Sau3AI digestion, providing conclusive evidence that the cassette includes a gene required for methylation of cytosine at GATC sites in both strains. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Genetic Characterization of Plasmid-Associated Benzalkonium Chloride Resistance Determinants in a Listeria monocytogenes Strain from the 1998-1999 Outbreak

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(24), 8231–8238.

By: D. Elhanafi n, V. Dutta* & S. Kathariou n

MeSH headings : Benzalkonium Compounds / pharmacology; DNA Transposable Elements; DNA, Bacterial / genetics; Disease Outbreaks; Disinfectants / pharmacology; Drug Resistance, Bacterial; Foodborne Diseases / epidemiology; Foodborne Diseases / microbiology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Complementation Test; Listeria monocytogenes / drug effects; Listeria monocytogenes / isolation & purification; Listeriosis / epidemiology; Listeriosis / microbiology; Plasmids; Transcription, Genetic; United States / epidemiology
TL;DR: A plasmid (pLM80)-associated BC resistance cassette is characterized in L. monocytogenes H7550, a strain implicated in the 1998-1999 multistate outbreak involving contaminated hot dogs and suggested to be harbored by an IS1216 composite transposon along with other genes whose functions are yet to be determined. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Genomic evidence for interspecies acquisition of chromosomal DNA from Campylobacter jejuni by Campylobacter coli strains of a turkey-associated clonal group (cluster II)

FOODBORNE PATHOGENS AND DISEASE, 5(4), 387–398.

By: K. Chan n, D. Elhanafi n & S. Kathariou n

MeSH headings : Animals; Bacterial Typing Techniques; Base Composition; Campylobacter coli / classification; Campylobacter coli / genetics; Campylobacter jejuni / classification; Campylobacter jejuni / genetics; Chromosomes, Bacterial / genetics; DNA, Bacterial / genetics; Gene Transfer, Horizontal; Genes, Bacterial; Genome, Bacterial; INDEL Mutation; Molecular Sequence Data; Open Reading Frames; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Sequence Alignment; Sequence Analysis, DNA; Turkeys / microbiology
TL;DR: The data suggest the occurrence of recombination events that resulted in genomic import of DNA from C. jejuni in the region between cydA and aspA in cluster II strains of C. coli. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Absence of serotype-specific surface antigen in laboratory variants of epidemic-associated Listeria monocytogenes strains

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(19), 6313–6316.

By: Y. Cheng n, L. Yue n, D. Elhanafi n & S. Kathariou n

MeSH headings : Antibodies, Monoclonal; Antigens, Bacterial / analysis; Antigens, Surface / analysis; Genes, Bacterial; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Listeria monocytogenes / immunology; Listeriosis / epidemiology; Listeriosis / microbiology; Serotyping
TL;DR: These findings suggest that variants that lacked reactivity with the serotype 4b-specific monoclonal antibody c74.22 can become inadvertently established under laboratory conditions and suggest caution in work involving serotypes 4b strains and genetic constructs thereof. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Validation of a green fluorescent protein-labeled strain of Vibrio vulnificus for use in the evaluation of postharvest strategies for handling of raw oysters

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(11), 7205–7211.

By: S. Drake n, D. Elhanafi n, W. Bang n, M. Drake n, D. Green n & L. Jaykus n

MeSH headings : Animals; Biomarkers / metabolism; Colony Count, Microbial; Culture Media; Food Handling / methods; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Hydrogen-Ion Concentration; Ostreidae / virology; Shellfish; Temperature; Time Factors; Vibrio vulnificus / genetics; Vibrio vulnificus / growth & development; Vibrio vulnificus / metabolism
TL;DR: Ease of detection and comparability to the wild-type parent make Vv-GFP a good candidate for use in studying the behavior of V. vulnificus upon exposure to sublethal stressors that might be encountered during postharvest handling of molluscan shellfish. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

A simple method for the direct detection of Salmonella and Escherichia coli O157 : H7 from raw alfalfa sprouts and spent irrigation water using PCR

JOURNAL OF FOOD PROTECTION, 68(11), 2256–2263.

By: L. Johnston n, D. Elhanafi n, M. Drake n & L. Jaykus n

MeSH headings : Colony Count, Microbial; Consumer Product Safety; DNA, Bacterial / analysis; Escherichia coli O157 / isolation & purification; Food Microbiology; Medicago sativa / microbiology; Polymerase Chain Reaction / methods; Salmonella / isolation & purification; Sensitivity and Specificity; Water Microbiology
TL;DR: Progress was made toward development of a rapid, inexpensive, and sensitive method for the detection of sprout-associated pathogens that is relevant to current industrial practices and needs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Effect of food matrix and cell growth on PCR-based detection of Escherichia coli O157 : H7 in ground beef

JOURNAL OF FOOD PROTECTION, 68(2), 225–232.

By: T. Taylor n, D. Elhanafi n, M. Drake n & L. Jaykus n

MeSH headings : Animals; Cattle; Colony Count, Microbial; DNA, Bacterial / analysis; Escherichia coli O157 / genetics; Escherichia coli O157 / growth & development; Escherichia coli O157 / isolation & purification; Food Contamination / analysis; Food Handling / methods; Food Microbiology; Hot Temperature; Meat Products / microbiology; Polymerase Chain Reaction / methods; Sensitivity and Specificity
TL;DR: Bacterial cell recovery and PCR detection limits were similar when comparing autoclaved ground beef and raw ground beef, but cell recoveries were highly variable for raw ground Beef samples in which E. coli O157:H7 cells were allowed to grow before processing for detection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Impact of cold and cold-acid stress on poststress tolerance and virulence factor expression of Escherichia coli O157: H7

Journal of Food Protection, 67(1), 19–26.

By: D. Elhanafi n, B. Leenanon n, W. Bang n & M. Drake n

MeSH headings : Adaptation, Physiological; Base Sequence; Colony Count, Microbial; Escherichia coli O157 / genetics; Escherichia coli O157 / pathogenicity; Escherichia coli O157 / physiology; Food Handling / methods; Food Microbiology; Freezing; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Restriction Mapping; Time Factors; Virulence / genetics
TL;DR: The results indicate that the prolonged storage of E. coli O157:H7 at 4 degrees C has substantial effects on freeze-thaw tolerance but does not affect subsequent virulence gene expression. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Acid adaptation and starvation effects on Shiga toxin production by Escherichia coli O157 : H7

JOURNAL OF FOOD PROTECTION, 66(6), 970–977.

By: B. Leenanon*, D. Elhanafi n & M. Drake n

MeSH headings : Adaptation, Physiological; Artificial Gene Fusion; Base Sequence; Colony Count, Microbial; Enzyme-Linked Immunosorbent Assay; Escherichia coli O157 / genetics; Escherichia coli O157 / isolation & purification; Escherichia coli O157 / physiology; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Mutation; RNA, Bacterial / analysis; RNA, Messenger / analysis; Reverse Transcriptase Polymerase Chain Reaction; Shiga Toxin 2 / biosynthesis; Shiga Toxin 2 / genetics
TL;DR: It is indicated that oxygen enhances stx-II mRNA expression and Stx production in E. coli O157:H7 and stress conditions such as acid adaptation and starvation enhance stX-II toxin mRNA levels but do not enhance subsequent Stx toxin production. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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