@article{zhu_rogers_heidary_huxford_2024, title={Structural and biochemical analyses of the nuclear IκBζ protein in complex with the NF-κB p50 homodimer}, volume={38}, ISSN={["1549-5477"]}, DOI={10.1101/gad.351892.124}, abstractNote={As part of the efforts to understand nuclear IκB function in NF-κB-dependent gene expression, we report an X-ray crystal structure of the IκBζ ankyrin repeat domain in complex with the dimerization domain of the NF-κB p50 homodimer. IκBζ possesses an N-terminal α helix that conveys domain folding stability. Affinity and specificity of the complex depend on a small portion of p50 at the nuclear localization signal. The model suggests that only one p50 subunit supports binding with IκBζ, and biochemical experiments confirm that IκBζ associates with DNA-bound NF-κB p50:RelA heterodimers. Comparisons of IκBζ:p50 and p50:κB DNA complex crystallographic models indicate that structural rearrangement is necessary for ternary complex formation of IκBζ and p50 with DNA.}, number={11-12}, journal={GENES & DEVELOPMENT}, author={Zhu, Norman and Rogers, W. Eric and Heidary, David K. and Huxford, Tom}, year={2024}, month={Jun}, pages={528–535} }
 @misc{havrylyuk_heidary_glazer_2024, title={The Impact of Inorganic Systems and Photoactive Metal Compounds on Cytochrome P450 Enzymes and Metabolism: From Induction to Inhibition}, volume={14}, ISSN={["2218-273X"]}, url={https://doi.org/10.3390/biom14040441}, DOI={10.3390/biom14040441}, abstractNote={While cytochrome P450 (CYP; P450) enzymes are commonly associated with the metabolism of organic xenobiotics and drugs or the biosynthesis of organic signaling molecules, they are also impacted by a variety of inorganic species. Metallic nanoparticles, clusters, ions, and complexes can alter CYP expression, modify enzyme interactions with reductase partners, and serve as direct inhibitors. This commonly overlooked topic is reviewed here, with an emphasis on understanding the structural and physiochemical basis for these interactions. Intriguingly, while both organometallic and coordination compounds can act as potent CYP inhibitors, there is little evidence for the metabolism of inorganic compounds by CYPs, suggesting a potential alternative approach to evading issues associated with rapid modification and elimination of medically useful compounds.}, number={4}, journal={BIOMOLECULES}, author={Havrylyuk, Dmytro and Heidary, David K. and Glazer, Edith C.}, year={2024}, month={Apr} }
 @article{swavey_heidary_boyd_erb_2023, title={Evaluation of Endoplasmic Reticulum Targeting Isoquinol-Based BODIPY Dyes and Their Properties as Molecular Rotors}, ISSN={["1099-0690"]}, DOI={10.1002/ejoc.202300777}, abstractNote={AbstractA series of six new isoquinol‐based BODIPY dyes have been synthesized by a two‐step method. Unlike traditional synthetic methods, synthesis did not require the use of oxidizing agents or solvent. Spectroscopic, electrochemical and DFT calculations indicate that the properties associated with the six dyes are very similar with little influence of the meso‐substituents. Viscosity studies identified three of the new dyes having properties of molecular rotors for applications in the detection of changes in cellular viscosity. Incubation of the dyes with the HEK cell line show no toxicity up to 10 μM over a 72 h. period. The dyes show the ability to localize within the endoplasmic reticulum (ER) of cells. With two of the dyes showing favorable comparisons to commercially available ER dyes.}, journal={EUROPEAN JOURNAL OF ORGANIC CHEMISTRY}, author={Swavey, Shawn and Heidary, David and Boyd, Emma and Erb, Jeremy}, year={2023}, month={Nov} }