@article{lee_caywood_lo_levering_keung_2021, title={Mapping the dynamic transfer functions of eukaryotic gene regulation}, volume={12}, ISSN={["2405-4720"]}, DOI={10.1016/j.cels.2021.08.003}, abstractNote={Biological information can be encoded within the dynamics of signaling components, which has been implicated in a broad range of physiological processes including stress response, oncogenesis, and stem cell differentiation. To study the complexity of information transfer across the eukaryotic promoter, we screened 119 dynamic conditions-modulating the pulse frequency, amplitude, and pulse width of light-regulating the binding of an epigenome editor to a fluorescent reporter. This system revealed tunable gene expression and filtering behaviors and provided a quantification of the limit to the amount of information that can be reliably transferred across a single promoter as ∼1.7 bits. Using a library of over 100 orthogonal chromatin regulators, we further determined that chromatin state could be used to tune mutual information and expression levels, as well as completely alter the input-output transfer function of the promoter. This system unlocks the information-rich content of eukaryotic gene regulation.}, number={11}, journal={CELL SYSTEMS}, author={Lee, Jessica B. and Caywood, Leandra M. and Lo, Jennifer Y. and Levering, Nicholas and Keung, Albert J.}, year={2021}, month={Nov}, pages={1079-+} }