2012 journal article

Avidity-mediated virus separation using a hyperthermophilic affinity ligand

BIOTECHNOLOGY PROGRESS, 29(1), 237–246.

By: M. Hussain n, D. Lockney n, R. Wang n, N. Gera n & B. Rao n

author keywords: avidity; affinity ligand; hyperthermophilic; multivalent
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / isolation & purification; DNA-Binding Proteins / metabolism; Ligands; Molecular Weight; Plant Extracts; Plant Leaves / virology; Sulfolobus solfataricus / chemistry; Sulfolobus solfataricus / metabolism; Tobacco / virology; Tombusviridae / isolation & purification
TL;DR: The approach may be broadly relevant to cases where capture of large multivalent species from complex mixtures and subsequent release without the use of harsh elution conditions is necessary, and bypasses chemical conjugation of the affinity ligand to the capture surface. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Mass Spectrometric Detection of Targeting Peptide Bioconjugation to Red clover necrotic mosaic virus

BIOCONJUGATE CHEMISTRY, 22(10), 1970–1982.

By: R. Wang, D. Lockney*, M. Goshe* & S. Franzen*

MeSH headings : Amino Acid Sequence; Capsid / chemistry; Cross-Linking Reagents / chemistry; Drug Delivery Systems; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Peptides / chemistry; Tombusviridae / chemistry
TL;DR: Results show that heterobifunctional linkers may not be the most efficient method for attachment of peptides to plant virus capsids, and maleimide peptides were used to conjugate with the virus in a one-step reaction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2010 journal article

The Red clover necrotic mosaic virus Capsid as a Multifunctional Cell Targeting Plant Viral Nanoparticle

BIOCONJUGATE CHEMISTRY, 22(1), 67–73.

By: D. Lockney n, R. Guenther n, L. Loo n, W. Overton n, R. Antonelli n, J. Clark n, M. Hu n, C. Luft n, S. Lommel n, S. Franzen n

MeSH headings : Amino Acid Sequence; Biological Availability; Biological Transport; Capsid / chemistry; Capsid / metabolism; Doxorubicin / chemistry; Doxorubicin / pharmacokinetics; Fluorescent Dyes / chemistry; HeLa Cells; Humans; Models, Molecular; Nanoparticles / chemistry; Peptides / chemistry; Peptides / metabolism; Plants / virology; Protein Conformation; Surface Properties; Tombusviridae
TL;DR: It is shown that targeting peptides (less than 16 amino acids) can be conjugated to the capsid using the heterobifunctional chemical linker sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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