@article{furgurson_loschin_butoto_abugu_gillespie_brown_ferraro_speicher_stokes_budnick_et al._2023, title={Seizing the policy moment in crop biotech regulation: an interdisciplinary response to the Executive Order on biotechnology}, volume={11}, ISSN={["2296-4185"]}, DOI={10.3389/fbioe.2023.1241537}, abstractNote={North Carolina State University Forestry and Environmental Resources, Raleigh, NC, United States, North Carolina State University Genetic Engineering and Society Center, Raleigh, NC, United States, North Carolina State University Applied Ecology, Raleigh, NC, United States, North Carolina State University Crop and Soil Sciences, Raleigh, NC, United States, North Carolina State University Horticultural Science, Raleigh, NC, United States, North Carolina State University Entomology and Plant Pathology, Raleigh, NC, United States, North Carolina State University Food, Bioprocessing and Nutrition Sciences, Raleigh, NC, United States, North Carolina State University Agricultural and Resource Economics, Raleigh, NC, United States, North Carolina State University Communication, Rhetoric, and Digital Media, Raleigh, NC, United States, North Carolina State University Biochemistry, Raleigh, NC, United States, North Carolina State University Plant Biology, Raleigh, NC, United States, North Carolina State University Anthropology, Raleigh, NC, United States}, journal={FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY}, author={Furgurson, Jill and Loschin, Nick and Butoto, Eric and Abugu, Modesta and Gillespie, Christopher J. and Brown, Rebekah and Ferraro, Greg and Speicher, Nolan and Stokes, Ruthie and Budnick, Asa and et al.}, year={2023}, month={Aug} }
 @article{butoto_brewer_holland_2022, title={Empirical comparison of genomic and phenotypic selection for resistance to Fusarium ear rot and fumonisin contamination in maize}, volume={7}, ISSN={["1432-2242"]}, url={https://doi.org/10.1007/s00122-022-04150-8}, DOI={10.1007/s00122-022-04150-8}, abstractNote={GS and PS performed similarly in improving resistance to FER and FUM content. With cheaper and faster genotyping methods, GS has the potential to be more efficient than PS. Fusarium verticillioides is a common maize (Zea mays L.) pathogen that causes Fusarium ear rot (FER) and produces the mycotoxin fumonisin (FUM). This study empirically compared phenotypic selection (PS) and genomic selection (GS) for improving FER and FUM resistance. Three intermating generations of recurrent GS were conducted in the same time frame and from a common base population as two generations of recurrent PS. Lines sampled from each PS and GS cycle were evaluated in three North Carolina environments in 2020. We observed similar cumulative responses to GS and PS, representing decreases of about 50% of mean FER and FUM compared to the base population. The first cycle of GS was more effective than later cycles. PS and GS both achieved about 70% of predicted total gain from selection for FER, but only about 26% of predicted gains for FUM, suggesting that heritability for FUM was overestimated. We observed a 20% decrease in genetic marker variation from PS and 30% decrease from GS. Our greatest challenge was our inability to quickly obtain dense and consistent set of marker genotypes across generations of GS. Practical implementation of GS in individual small-scale breeding programs will require cheaper and faster genotyping methods, and such technological advances will present opportunities to significantly optimize selection and mating schemes for future GS efforts beyond what we were able to achieve in this study.}, journal={THEORETICAL AND APPLIED GENETICS}, author={Butoto, Eric N. and Brewer, Jason C. and Holland, James B.}, year={2022}, month={Jul} }
 @article{butoto_marino_holland_2021, title={Effects of artificial inoculation on trait correlations with resistance to Fusarium ear rot and fumonisin contamination in maize}, volume={5}, ISSN={["1435-0653"]}, url={https://doi.org/10.1002/csc2.20551}, DOI={10.1002/csc2.20551}, abstractNote={AbstractBreeding for resistance to Fusarium ear rot (FER; Fusarium verticillioides Nirenberg) and fumonisin (FUM) contamination in maize (Zea mays L.) is labor intensive, time consuming, and expensive. The objective of this study is to explore three possible shortcuts to improve the efficiency of breeding for resistance to FER and FUM: relying on natural instead of artificial inoculation of Fusarium verticillioides, selecting inbred lines per se instead of topcross hybrids, and using grain test weight (TW) as an indirect selection criterion. We selected the 27 most resistant and 26 most susceptible S0:1 lines from the third cycle of a broad‐based recurrent selection population and topcrossed them to a common inbred line. The resulting topcross hybrids were evaluated in three North Carolina environments under artificial and natural inoculation of F. verticillioides. The entry‐mean heritabilities for FER and FUM are considerably reduced under natural conditions compared to artificial inoculation; therefore, artificial inoculation should be practiced for efficient selection for resistance. We found a high correlation between FER and FUM content of S0:1 lines per se and their topcross hybrids, suggesting that selection among early generation inbred lines per se is an efficient method to improve resistance in their topcross hybrids. The TW of inoculated S0:1 lines per se was strongly and negatively correlated with FER and FUM of their topcross hybrids, suggesting that TW can be utilized as an indirect selection criterion to improve resistance to FER and FUM contamination.}, journal={CROP SCIENCE}, publisher={Wiley}, author={Butoto, Eric N. and Marino, Thiago P. and Holland, James B.}, year={2021}, month={Jun} }