Works (8)
Updated: July 6th, 2023 21:17
2019 journal article
NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods
Molecular & Cellular Proteomics, 19(1), 11–30.
author keywords: Glycomics; mass spectrometry; fluorescence; glycosylation; glycoproteins; glycan; glycopeptide; interlaboratory study; NISTmAb; reference antibody
MeSH headings : Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / metabolism; Biological Products; Biopharmaceutics / methods; Glycomics / methods; Glycopeptides / metabolism; Glycosylation; Humans; Laboratories; Polysaccharides / metabolism; Protein Processing, Post-Translational; Proteomics / methods
TL;DR:
A broad-based interlaboratory study of glycosylation profiles of a reference and modified IgG antibody involving 103 reports from 76 laboratories offers a view of the current state-of-the-art for biologic glyCosylation measurement and suggests a clear need for harmonization of gly cosylation analysis methods.
(via Semantic Scholar)
open access via www.mcponline.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries
Added: May 11, 2020
2017 journal article
DRILL: An Electrospray Ionization-Mass Spectrometry Interface for Improved Sensitivity via Inertial Droplet Sorting and Electrohydrodynamic Focusing in a Swirling Flow
ANALYTICAL CHEMISTRY, 89(17), 8981–8987.
MeSH headings : Angiotensin I / analysis; Angiotensin I / metabolism; Angiotensin II / analysis; Angiotensin II / metabolism; Chromatography, High Pressure Liquid; Humans; Isotope Labeling; Limit of Detection; Peptides / analysis; Peptides / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR:
This work experimentally demonstrates sensitivity improvement obtained via vortex-induced inertial sorting of electrosprayed droplets/ions: one possible mode of DRILL operation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2017 journal article
Xylose Migration During Tandem Mass Spectrometry of N-Linked Glycans
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 28(4), 729–732.
author keywords: Xylose migration; MS/MS; Glycomics; N-linked glycans; Plants
TL;DR:
This work reports the second instance of saccharide migration ever to occur for N-glycans, the first empirical observation of this rearrangement, and warrants dissemination so it may be searched in de novo sequencing glycan workflows.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
A quantitative glycomics and proteomics combined purification strategy
Jove-Journal of Visualized Experiments, (109).
Source: NC State University Libraries
Added: August 6, 2018
2016 journal article
N-linked glycosite profiling and use of Skyline as a platform for characterization and relative quantification of glycans in differentiating xylem of Populus trichocarpa
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 409(2), 487–497.
author keywords: N-linked glycosite; Skyline; Populus trichocarpa; Monolignol; N-linked glycans
MeSH headings : Computational Biology / standards; Computational Biology / trends; Polysaccharides / analysis; Populus / chemistry; Xylem / chemistry
TL;DR:
This study presents a novel workflow for identification of glycans using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy developed in the lab, and implements Skyline for relative glycan quantification for improved accuracy and precision of quantitative measurements over current processing tools.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 review
Optimizing Mass Spectrometry Analyses: A Tailored Review on the Utility of Design of Experiments
[Review of ]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 27(5), 767–785.
author keywords: Design of experiments; Mass spectrometry; Tutorial; Optimization; Modeling
MeSH headings : Mass Spectrometry; Models, Statistical; Research Design
TL;DR:
This review provides a tutorial-like guide into the selection of DOE for MS experiments, the practices for modeling and optimization of response variables, and the available software tools that support DOE implementation in any laboratory.
(via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Definitive Screening Design Optimization of Mass Spectrometry Parameters for Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT Plasma N-Glycans
ANALYTICAL CHEMISTRY, 87(14), 7305–7312.
MeSH headings : Animals; Chickens; Equipment Design; Mass Spectrometry / instrumentation; Polysaccharides / blood; Polysaccharides / isolation & purification; Solid Phase Extraction / instrumentation
TL;DR:
High-throughput, quantitative processing of N-linked glycans would facilitate large-scale studies correlating the glycome with disease and open the field to basic and applied researchers by coupling filter-aided-N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags (INLIGHT) for analysis of plasma.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Relative Quantification and Higher-Order Modeling of the Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples
JOURNAL OF PROTEOME RESEARCH, 14(10), 4394–4401.
author keywords: N-linked glycosylation; INLIGHT; ovarian cancer; relative quantification; cancer biomarker; human plasma
MeSH headings : Biomarkers, Tumor / blood; Carbohydrate Sequence; Case-Control Studies; Chromatography, Liquid / methods; Female; Fucose / blood; Glycosylation; Humans; Hydrazines / chemistry; Molecular Sequence Data; Neoplasm Staging; Ovarian Neoplasms / blood; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / pathology; Polysaccharides / blood; Sialic Acids / blood; Staining and Labeling / methods; Tandem Mass Spectrometry / methods
TL;DR:
For all N-glycans, the vectors of the effects from stages II-IV were sequentially reversed, suggesting potential biological changes in OVC morphology or in host response.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
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