Elizabeth S. Hecht

Works (8)

Updated: July 6th, 2023 21:17

2019 journal article

NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods

Molecular & Cellular Proteomics, 19(1), 11–30.

By: D. Muddiman  & E. Hecht (2 connected NC State authors and many others, 162 authors in total )

author keywords: Glycomics; mass spectrometry; fluorescence; glycosylation; glycoproteins; glycan; glycopeptide; interlaboratory study; NISTmAb; reference antibody
MeSH headings : Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / metabolism; Biological Products; Biopharmaceutics / methods; Glycomics / methods; Glycopeptides / metabolism; Glycosylation; Humans; Laboratories; Polysaccharides / metabolism; Protein Processing, Post-Translational; Proteomics / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Monoclonal and Polyclonal Antibodies Research; Analytical Chemistry and Chromatography
TL;DR: A broad-based interlaboratory study of glycosylation profiles of a reference and modified IgG antibody involving 103 reports from 76 laboratories offers a view of the current state-of-the-art for biologic glyCosylation measurement and suggests a clear need for harmonization of gly cosylation analysis methods. (via Semantic Scholar)
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Sources: Crossref, NC State University Libraries
Added: May 11, 2020

2017 article

DRILL: An Electrospray Ionization-Mass Spectrometry Interface for Improved Sensitivity via Inertial Droplet Sorting and Electrohydrodynamic Focusing in a Swirling Flow

Kottke, P. A., Lee, J. Y., Jonke, A. P., Seneviratne, C. A., Hecht, E. S., Muddiman, D. C., … Fedorov, A. G. (2017, June 14). Analytical Chemistry, Vol. 89, pp. 8981–8987.

By: P. Kottke *, J. Lee *, A. Jonke*, C. Seneviratne  n, E. Hecht n, D. Muddiman n , M. Torres *, A. Fedorov *

MeSH headings : Angiotensin I / analysis; Angiotensin I / metabolism; Angiotensin II / analysis; Angiotensin II / metabolism; Chromatography, High Pressure Liquid; Humans; Isotope Labeling; Limit of Detection; Peptides / analysis; Peptides / chemistry; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Electrohydrodynamics and Fluid Dynamics; Laser-induced spectroscopy and plasma
TL;DR: This work experimentally demonstrates sensitivity improvement obtained via vortex-induced inertial sorting of electrosprayed droplets/ions: one possible mode of DRILL operation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 article

Xylose Migration During Tandem Mass Spectrometry of N-Linked Glycans

Hecht, E. S., Loziuk, P. L., & Muddiman, D. C. (2017, January 26). Journal of the American Society for Mass Spectrometry, Vol. 28, pp. 729–732.

By: E. Hecht n, P. Loziuk n & D. Muddiman n 

author keywords: Xylose migration; MS/MS; Glycomics; N-linked glycans; Plants
topics (OpenAlex): Glycosylation and Glycoproteins Research; Carbohydrate Chemistry and Synthesis; Genomics and Phylogenetic Studies
TL;DR: This work reports the second instance of saccharide migration ever to occur for N-glycans, the first empirical observation of this rearrangement, and warrants dissemination so it may be searched in de novo sequencing glycan workflows. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

A quantitative glycomics and proteomics combined purification strategy

Jove-Journal of Visualized Experiments, (109).

By: E. Hecht, J. McCord & D. Muddiman 

Source: NC State University Libraries
Added: August 6, 2018

2016 article

N-linked glycosite profiling and use of Skyline as a platform for characterization and relative quantification of glycans in differentiating xylem of Populus trichocarpa

Loziuk, P. L., Hecht, E. S., & Muddiman, D. C. (2016, August 4). Analytical and Bioanalytical Chemistry, Vol. 409, pp. 487–497.

By: P. Loziuk n, E. Hecht n & D. Muddiman n 

author keywords: N-linked glycosite; Skyline; Populus trichocarpa; Monolignol; N-linked glycans
MeSH headings : Computational Biology / standards; Computational Biology / trends; Polysaccharides / analysis; Populus / chemistry; Xylem / chemistry
topics (OpenAlex): Biofuel production and bioconversion; Glycosylation and Glycoproteins Research; Plant Gene Expression Analysis
TL;DR: This study presents a novel workflow for identification of glycans using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy developed in the lab, and implements Skyline for relative glycan quantification for improved accuracy and precision of quantitative measurements over current processing tools. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 article

Optimizing Mass Spectrometry Analyses: A Tailored Review on the Utility of Design of Experiments

Hecht, E. S., Oberg, A. L., & Muddiman, D. C. (2016, March 7). Journal of the American Society for Mass Spectrometry, Vol. 27, pp. 767–785.

By: E. Hecht n, A. Oberg * & D. Muddiman n 

author keywords: Design of experiments; Mass spectrometry; Tutorial; Optimization; Modeling
MeSH headings : Mass Spectrometry; Models, Statistical; Research Design
topics (OpenAlex): Mass Spectrometry Techniques and Applications; Pesticide Residue Analysis and Safety; Analytical Chemistry and Chromatography
TL;DR: This review provides a tutorial-like guide into the selection of DOE for MS experiments, the practices for modeling and optimization of response variables, and the available software tools that support DOE implementation in any laboratory. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Definitive Screening Design Optimization of Mass Spectrometry Parameters for Sensitive Comparison of Filter and Solid Phase Extraction Purified, INLIGHT PlasmaN-Glycans

Hecht, E. S., McCord, J. P., & Muddiman, D. C. (2015, June 18). Analytical Chemistry, Vol. 87, pp. 7305–7312.

By: E. Hecht n, J. McCord n & D. Muddiman n 

MeSH headings : Animals; Chickens; Equipment Design; Mass Spectrometry / instrumentation; Polysaccharides / blood; Polysaccharides / isolation & purification; Solid Phase Extraction / instrumentation
topics (OpenAlex): Glycosylation and Glycoproteins Research; Mass Spectrometry Techniques and Applications; Carbohydrate Chemistry and Synthesis
TL;DR: High-throughput, quantitative processing of N-linked glycans would facilitate large-scale studies correlating the glycome with disease and open the field to basic and applied researchers by coupling filter-aided-N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags (INLIGHT) for analysis of plasma. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 article

Relative Quantification and Higher-Order Modeling of the Plasma Glycan Cancer Burden Ratio in Ovarian Cancer Case-Control Samples

Hecht, E. S., Scholl, E. H., Walker, S. H., Taylor, A. D., Cliby, W. A., Motsinger-Reif, A. A., & Muddiman, D. C. (2015, September 8). Journal of Proteome Research, Vol. 14, pp. 4394–4401.

By: E. Hecht*, E. Scholl*, S. Walker*, A. Taylor, W. Cliby *, A. Motsinger-Reif*, D. Muddiman* 

author keywords: N-linked glycosylation; INLIGHT; ovarian cancer; relative quantification; cancer biomarker; human plasma
MeSH headings : Biomarkers, Tumor / blood; Carbohydrate Sequence; Case-Control Studies; Chromatography, Liquid / methods; Female; Fucose / blood; Glycosylation; Humans; Hydrazines / chemistry; Molecular Sequence Data; Neoplasm Staging; Ovarian Neoplasms / blood; Ovarian Neoplasms / diagnosis; Ovarian Neoplasms / pathology; Polysaccharides / blood; Sialic Acids / blood; Staining and Labeling / methods; Tandem Mass Spectrometry / methods
topics (OpenAlex): Glycosylation and Glycoproteins Research; Monoclonal and Polyclonal Antibodies Research; Lung Cancer Research Studies
TL;DR: For all N-glycans, the vectors of the effects from stages II-IV were sequentially reversed, suggesting potential biological changes in OVC morphology or in host response. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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