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Works (14)
Updated: September 4th, 2024 05:01
2024 journal article
Antennal transcriptome analysis reveals sensory receptors potentially associated with host detection in the livestock pest <i>Lucilia cuprina</i>
PARASITES & VECTORS, 17(1).
author keywords: Myiasis; Blowfly livestock pest; Australian sheep blowfly; Host seeking; RNA-Seq; DESeq2; Chemoreceptor; Mechanoreceptor; Antennae
open access via doi.org (publisher)
Sources: Web Of Science, NC State University Libraries
Added: July 30, 2024
2024 journal article
Identification of a gene promoter active in<i> Lucilia</i><i> sericata</i> larval salivary glands using a rapid transient expression assay
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 173.
author keywords: Salivary gland; Promoter; Maggot therapy; Malpighian tubules; Lucilia sericata
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 1, 2024
2021 journal article
A conditional female lethal system for genetic suppression of the global fruit crop pest <scp><i>Drosophila suzukii
Pest Management Science, 77(11), 4915–4922.
author keywords: Drosophila suzukii; spotted wing Drosophila; sterile insect technique; fsRIDL; tet-OFF; alternate splicing
MeSH headings : Animals; Drosophila / genetics; Fruit; Housing, Animal; Rubus; Suppression, Genetic
TL;DR:
It is shown that repeated releases of an excess of transgenic males can suppress D. suzukii populations in laboratory cage trials and suggests that the transgenic strain could provide an effective approach for control of this invasive pest of soft-skinned fruits.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: July 19, 2021
2021 journal article
Genome and transcriptome sequencing of the green bottle fly, Lucilia sericata, reveals underlying factors of sheep flystrike and maggot debridement therapy
Genomics, 113(6), 3978–3988.
author keywords: Lucilia sericata; Maggot debridement therapy; Whole genome; Salivary gland; Malpighian tubule; Heat shock
MeSH headings : Animals; Calliphoridae; Debridement; Diptera / genetics; Humans; Larva / genetics; Larva / metabolism; Sheep / genetics; Transcriptome
TL;DR:
The assembly of a 565.3 Mb genome from long read PacBio DNA sequencing of genomic DNA is reported, which will underpin future genetic studies and in development of engineered strains for genetic control of L. sericata and for biotechnology-enhanced maggot therapy.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: October 26, 2021
2019 journal article
Specific Gene Disruption in the Major Livestock Pests<i>Cochliomyia hominivorax</i>and<i>Lucilia cuprina</i>Using CRISPR/Cas9
G3 Genes|Genomes|Genetics, 9(9), 3045–3055.
author keywords: Functional genomics; reverse genetics; myiasis; New World Screwworm fly; Australian Sheep Blowfly; gene drive; CRISPR; Cas9; brown body; yellow; transformer
MeSH headings : Animals; Animals, Genetically Modified; CRISPR-Cas Systems; Diptera / embryology; Diptera / genetics; Embryo, Nonmammalian; Female; Insect Proteins / genetics; Male; Mutation; Pest Control / methods; RNA, Guide, Kinetoplastida; Sex Determination Processes
TL;DR:
The CRISPR protocol described here is a significant improvement on the existing toolkit of molecular methods in calliphorids and suggests that Cas9-based systems targeting Chtra and Lctra could be an effective means for controlling natural populations of these important pests.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: September 23, 2019
2018 journal article
Towards next generation maggot debridement therapy: Transgenic Lucilia sericata larvae that produce and secrete a human growth factor
Wound Repair and Regeneration, 26(1), A27–27.
Source: NC State University Libraries
Added: August 6, 2018
2018 journal article
no blokes Is Essential for Male Viability and X Chromosome Gene Expression in the Australian Sheep Blowfly
Current Biology, 28(12), 1987–1992.e3.
MeSH headings : Animals; Diptera / genetics; Diptera / physiology; Dosage Compensation, Genetic / genetics; Female; Gene Expression; Genes, Insect / genetics; Genes, X-Linked / genetics; Male; X Chromosome / genetics
TL;DR:
This study supports the hypothesis on the origin of the Drosophila 4th chromosome and that a POF-like protein was required for normal gene expression on the ancient X chromosome and suggests that NBL could be important for X chromosome dosage compensation in L. cuprina.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018
2016 journal article
Towards next generation maggot debridement therapy: transgenic Lucilia sericata larvae that produce and secrete a human growth factor
BMC Biotechnology, 16(1).
author keywords: Maggot debridement therapy (MDT); Platelet-derived growth factor (PDGF); Excretions/secretions (ES); Growth factor treatment; Diabetic foot ulcer; Tetracycline transactivator; Lucilia sericata; Wound healing
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018
2015 journal article
Dosage Compensation of X-Linked Muller Element F Genes but Not X-Linked Transgenes in the Australian Sheep Blowfly
PLOS ONE, 10(10), e0141544.
Ed(s): G. Marais
MeSH headings : Animals; Animals, Genetically Modified; Diptera / genetics; Dosage Compensation, Genetic; Drosophila melanogaster / genetics; Female; Gene Expression Regulation, Developmental; Genes, X-Linked / genetics; Larva; Male; X Chromosome / genetics
TL;DR:
It appears that the Lucilia dosage compensation system is specific for genes endogenous to the X chromosome and cannot be co-opted by recently arrived transgenes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2014 journal article
Functional characterization of calliphorid cell death genes and cellularization gene promoters for controlling gene expression and cell viability in early embryos
Insect Molecular Biology, 24(1), 58–70.
author keywords: genetic sexing; transgenic; cellularization gene; sterile insect technique
MeSH headings : Amino Acid Sequence; Animals; Animals, Genetically Modified; Base Sequence; Cell Death / genetics; Cell Survival; Diptera / embryology; Diptera / genetics; Diptera / growth & development; Drosophila melanogaster / genetics; Drosophila melanogaster / growth & development; Embryo, Nonmammalian; Female; Gene Expression Regulation; Genes, Insect; Genes, Lethal; Male; Molecular Sequence Data; Pest Control, Biological / methods; Promoter Regions, Genetic; Sex Ratio
TL;DR:
The isolated gene promoters and pro‐apoptotic genes could potentially be used to build transgenic embryonic sexing strains of calliphorid livestock pests.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2014 journal article
Transgenic sexing system for genetic control of the Australian sheep blow fly Lucilia cuprina
Insect Biochemistry and Molecular Biology, 51, 80–88.
author keywords: Sterile insect technique; Transgenic; Lucilia cuprina; Flystrike; Genetic sexing
MeSH headings : Animals; Animals, Genetically Modified; Diptera / genetics; Female; Genes, Insect; Genes, Lethal; Male; Pest Control, Biological / methods; Sex Determination Processes
TL;DR:
Transgenic strains of L. cuprina that carry a dominant tetracycline repressible female lethal genetic system are developed and should be readily transferrable to other major calliphorid livestock pests including the New and Old World screwworm.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018
2013 journal article
Conservation and Sex-Specific Splicing of the transformer Gene in the Calliphorids Cochliomyia hominivorax, Cochliomyia macellaria and Lucilia sericata
PLoS ONE, 8(2), e56303.
Ed(s): G. Tear
MeSH headings : Animals; Base Sequence; Conserved Sequence / genetics; Diptera / genetics; Diptera / growth & development; Diptera / physiology; Female; Genes, Insect / genetics; Male; Molecular Sequence Data; Nucleotide Motifs / genetics; RNA Splicing / genetics; RNA, Messenger / genetics; RNA, Messenger / metabolism; Sex Characteristics
TL;DR:
A model for sex specific regulation of tra splicing is proposed that incorporates the conserved features identified in this study and is proposed to facilitate the development of a “male-only” strain for genetic control programs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2012 journal article
Organization and expression of the Australian sheep blowfly (<i>Lucilia cuprina</i>) <i>hsp23</i>, <i>hsp24, hsp70</i> and <i>hsp83</i> genes
Insect Molecular Biology, 21(2), 169–180.
author keywords: heat shock; hsp70; hsp23; hsp83; Lucilia cuprina; HSF
MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Conserved Sequence; Diptera / genetics; Diptera / growth & development; Diptera / metabolism; Female; Gene Expression; Genes, Insect; Heat-Shock Proteins / genetics; Heat-Shock Proteins / metabolism; Hot Temperature; Insect Proteins / genetics; Insect Proteins / metabolism; Male; Molecular Sequence Data; Phylogeny; Promoter Regions, Genetic; Sequence Alignment
TL;DR:
The isolation and characterization of a heat shock protein 70 (hsp70) gene, the hsp83 gene and two genes that encode small Hsps from the Australian sheep blowfly, Lucilia cuprina, a major agricultural pest are reported.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
2010 journal article
Sex-biased transcription enhancement by a 5' tethered Gal4-MOF histone acetyltransferase fusion protein in Drosophila
BMC Molecular Biology, 11(1).
MeSH headings : Acetylation; Amino Acid Sequence; Animals; DNA-Binding Proteins / analysis; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Drosophila / enzymology; Drosophila / genetics; Drosophila / metabolism; Drosophila Proteins / analysis; Drosophila Proteins / genetics; Drosophila Proteins / metabolism; Female; Genes, Reporter; Genes, X-Linked; Histone Acetyltransferases / analysis; Histone Acetyltransferases / genetics; Histone Acetyltransferases / metabolism; Histones / genetics; Histones / metabolism; Male; Models, Molecular; Molecular Sequence Data; Nuclear Proteins / analysis; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Polytene Chromosomes / chemistry; Recombinant Fusion Proteins / analysis; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism; Sequence Alignment; Sex Factors; Transcription Factors / analysis; Transcription Factors / genetics; Transcription Factors / metabolism; Transcriptional Activation
TL;DR:
Targeting MOF to reporter genes led to transcription enhancement and acetylation of histone H4 at lysine 16 and how association of Gal4-MOF with the MSL or NSL proteins could explain the results is discussed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, Crossref
Added: August 6, 2018
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