@article{burris_makarem_slabaugh_chaves_pierce_lee_kiemle_kwansa_singh_yingling_et al._2021, title={Phenotypic effects of changes in the FTVTxK region of an Arabidopsis secondary wall cellulose synthase compared with results from analogous mutations in other isoforms}, volume={5}, ISSN={["2475-4455"]}, url={https://doi.org/10.1002/pld3.335}, DOI={10.1002/pld3.335}, abstractNote={Abstract}, number={8}, journal={PLANT DIRECT}, publisher={Wiley}, author={Burris, Jason N. and Makarem, Mohamadamin and Slabaugh, Erin and Chaves, Arielle and Pierce, Ethan T. and Lee, Jongcheol and Kiemle, Sarah N. and Kwansa, Albert L. and Singh, Abhishek and Yingling, Yaroslava G. and et al.}, year={2021}, month={Aug} }
 @article{slabaugh_desai_sartor_lawas_krishna jagadish_doherty_2019, title={Analysis of differential gene expression and alternative splicing is significantly influenced by choice of reference genome}, volume={25}, ISSN={["1469-9001"]}, url={https://doi.org/10.1261/rna.070227.118}, DOI={10.1261/rna.070227.118}, abstractNote={RNA-seq analysis has enabled the evaluation of transcriptional changes in many species including nonmodel organisms. However, in most species only a single reference genome is available and RNA-seq reads from highly divergent varieties are typically aligned to this reference. Here, we quantify the impacts of the choice of mapping genome in rice where three high-quality reference genomes are available. We aligned RNA-seq data from a popular productive rice variety to three different reference genomes and found that the identification of differentially expressed genes differed depending on which reference genome was used for mapping. Furthermore, the ability to detect differentially used transcript isoforms was profoundly affected by the choice of reference genome: Only 30% of the differentially used splicing features were detected when reads were mapped to the more commonly used, but more distantly related reference genome. This demonstrated that gene expression and splicing analysis varies considerably depending on the mapping reference genome, and that analysis of individuals that are distantly related to an available reference genome may be improved by acquisition of new genomic reference material. We observed that these differences in transcriptome analysis are, in part, due to the presence of single nucleotide polymorphisms between the sequenced individual and each respective reference genome, as well as annotation differences between the reference genomes that exist even between syntenic orthologs. We conclude that even between two closely related genomes of similar quality, using the reference genome that is most closely related to the species being sampled significantly improves transcriptome analysis.}, number={6}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={Slabaugh, Erin and Desai, Jigar S. and Sartor, Ryan C. and Lawas, Lovely Mae F. and Krishna Jagadish, S. V. and Doherty, Colleen J.}, year={2019}, month={Jun}, pages={669–684} }
 @article{desai_slabaugh_liebelt_fredenberg_gray_jagadish_wilkins_doherty_2018, title={Neural Net Classification Combined With Movement Analysis to Evaluate Setaria viridis as a Model System for Time of Day of Anther Appearance}, volume={9}, ISSN={["1664-462X"]}, url={http://dx.doi.org/10.3389/fpls.2018.01585}, DOI={10.3389/fpls.2018.01585}, abstractNote={In many plant species, the time of day at which flowers open to permit pollination is tightly regulated. Proper time of flower opening, or Time of Day of Anther Appearance (TAA), may coordinate flowering opening with pollinator activity or may shift temperature sensitive developmental processes to cooler times of the day. The genetic mechanisms that regulate the timing of this process in cereal crops are unknown. To address this knowledge gap, it is necessary to establish a monocot model system that exhibits variation in TAA. Here, we examine the suitability of Setaria viridis, the model for C4 photosynthesis, for such a role. We developed an imaging system to monitor the temporal regulation of growth, flower opening time, and other physiological characteristics in Setaria. This system enabled us to compare Setaria varieties Ames 32254, Ames 32276, and PI 669942 variation in growth and daily flower opening time. We observed that TAA occurs primarily at night in these three Setaria accessions. However, significant variation between the accessions was observed for both the ratio of flowers that open in the day vs. night and the specific time of day where the rate is maximal. Characterizing this physiological variation is a requisite step toward uncovering the molecular mechanisms regulating TAA. Leveraging the regulation of TAA could provide researchers with a genetic tool to improve crop productivity in new environments.}, journal={FRONTIERS IN PLANT SCIENCE}, author={Desai, Jigar S. and Slabaugh, Erin and Liebelt, Donna J. and Fredenberg, Jacob D. and Gray, Benjamin N. and Jagadish, S. V. Krishna and Wilkins, Olivia and Doherty, Colleen J.}, year={2018}, month={Oct} }
 @article{renna_stefano_slabaugh_wormsbaecher_sulpizio_zienkiewicz_brandizzi_2018, title={TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network}, volume={9}, ISSN={["2041-1723"]}, DOI={10.1038/s41467-018-07662-4}, abstractNote={Abstract}, journal={NATURE COMMUNICATIONS}, author={Renna, Luciana and Stefano, Giovanni and Slabaugh, Erin and Wormsbaecher, Clarissa and Sulpizio, Alan and Zienkiewicz, Krzysztof and Brandizzi, Federica}, year={2018}, month={Dec} }
 @article{nixon_mansouri_singh_du_davis_lee_slabaugh_vandavasi_o’neill_roberts_et al._2016, title={Comparative Structural and Computational Analysis Supports Eighteen Cellulose Synthases in the Plant Cellulose Synthesis Complex}, volume={6}, ISSN={2045-2322}, url={http://dx.doi.org/10.1038/srep28696}, DOI={10.1038/srep28696}, abstractNote={Abstract}, number={1}, journal={Scientific Reports}, publisher={Springer Science and Business Media LLC}, author={Nixon, B. Tracy and Mansouri, Katayoun and Singh, Abhishek and Du, Juan and Davis, Jonathan K. and Lee, Jung-Goo and Slabaugh, Erin and Vandavasi, Venu Gopal and O’Neill, Hugh and Roberts, Eric M. and et al.}, year={2016}, month={Jun} }
 @article{sethaphong_davis_slabaugh_singh_haigler_yingling_2016, title={Prediction of the structures of the plant-specific regions of vascular plant cellulose synthases and correlated functional analysis}, volume={23}, ISSN={0969-0239 1572-882X}, url={http://dx.doi.org/10.1007/s10570-015-0789-6}, DOI={10.1007/s10570-015-0789-6}, number={1}, journal={Cellulose}, publisher={Springer Science and Business Media LLC}, author={Sethaphong, Latsavongsakda and Davis, Jonathan K. and Slabaugh, Erin and Singh, Abhishek and Haigler, Candace H. and Yingling, Yaroslava G.}, year={2016}, month={Feb}, pages={145–161} }
 @article{slabaugh_scavuzzo-duggan_chaves_wilson_wilson_davis_cosgrove_anderson_roberts_haigler_2015, title={The valine and lysine residues in the conserved FxVTxK motif are important for the function of phylogenetically distant plant cellulose synthases}, volume={26}, ISSN={0959-6658 1460-2423}, url={http://dx.doi.org/10.1093/glycob/cwv118}, DOI={10.1093/glycob/cwv118}, abstractNote={Cellulose synthases (CESAs) synthesize the β-1,4-glucan chains that coalesce to form cellulose microfibrils in plant cell walls. In addition to a large cytosolic (catalytic) domain, CESAs have eight predicted transmembrane helices (TMHs). However, analogous to the structure of BcsA, a bacterial CESA, predicted TMH5 in CESA may instead be an interfacial helix. This would place the conserved FxVTxK motif in the plant cell cytosol where it could function as a substrate-gating loop as occurs in BcsA. To define the functional importance of the CESA region containing FxVTxK, we tested five parallel mutations in Arabidopsis thaliana CESA1 and Physcomitrella patens CESA5 in complementation assays of the relevant cesa mutants. In both organisms, the substitution of the valine or lysine residues in FxVTxK severely affected CESA function. In Arabidopsis roots, both changes were correlated with lower cellulose anisotropy, as revealed by Pontamine Fast Scarlet. Analysis of hypocotyl inner cell wall layers by atomic force microscopy showed that two altered versions of Atcesa1 could rescue cell wall phenotypes observed in the mutant background line. Overall, the data show that the FxVTxK motif is functionally important in two phylogenetically distant plant CESAs. The results show that Physcomitrella provides an efficient model for assessing the effects of engineered CESA mutations affecting primary cell wall synthesis and that diverse testing systems can lead to nuanced insights into CESA structure-function relationships. Although CESA membrane topology needs to be experimentally determined, the results support the possibility that the FxVTxK region functions similarly in CESA and BcsA.}, number={5}, journal={Glycobiology}, publisher={Oxford University Press (OUP)}, author={Slabaugh, Erin and Scavuzzo-Duggan, Tess and Chaves, Arielle and Wilson, Liza and Wilson, Carmen and Davis, Jonathan K and Cosgrove, Daniel J and Anderson, Charles T and Roberts, Alison W and Haigler, Candace H}, year={2015}, month={Dec}, pages={509–519} }
 @article{slabaugh_davis_haigler_yingling_zimmer_2014, title={Cellulose synthases: new insights from crystallography and modeling}, volume={19}, ISSN={1360-1385}, url={http://dx.doi.org/10.1016/j.tplants.2013.09.009}, DOI={10.1016/j.tplants.2013.09.009}, abstractNote={•A crystal structure and a modeled structure of cellulose synthases are examined. •We explore similarities/differences between bacterial and plant cellulose synthase. •Molecular mechanisms for known cellulose synthase missense mutations are proposed. •We predict specific residues putatively involved in glucan translocation in plants. Detailed information about the structure and biochemical mechanisms of cellulose synthase (CelS) proteins remained elusive until a complex containing the catalytic subunit (BcsA) of CelS from Rhodobacter sphaeroides was crystalized. Additionally, a 3D structure of most of the cytosolic domain of a plant CelS (GhCESA1 from cotton, Gossypium hirsutum) was produced by computational modeling. This predicted structure contributes to our understanding of how plant CelS proteins may be similar and different as compared with BcsA. In this review, we highlight how these structures impact our understanding of the synthesis of cellulose and other extracellular polysaccharides. We show how the structures can be used to generate hypotheses for experiments testing mechanisms of glucan synthesis and translocation in plant CelS. Detailed information about the structure and biochemical mechanisms of cellulose synthase (CelS) proteins remained elusive until a complex containing the catalytic subunit (BcsA) of CelS from Rhodobacter sphaeroides was crystalized. Additionally, a 3D structure of most of the cytosolic domain of a plant CelS (GhCESA1 from cotton, Gossypium hirsutum) was produced by computational modeling. This predicted structure contributes to our understanding of how plant CelS proteins may be similar and different as compared with BcsA. In this review, we highlight how these structures impact our understanding of the synthesis of cellulose and other extracellular polysaccharides. We show how the structures can be used to generate hypotheses for experiments testing mechanisms of glucan synthesis and translocation in plant CelS.}, number={2}, journal={Trends in Plant Science}, publisher={Elsevier BV}, author={Slabaugh, Erin and Davis, Jonathan K. and Haigler, Candace H. and Yingling, Yaroslava G. and Zimmer, Jochen}, year={2014}, month={Feb}, pages={99–106} }
 @article{slabaugh_sethaphong_xiao_amick_anderson_haigler_yingling_2014, title={Computational and genetic evidence that different structural conformations of a non-catalytic region affect the function of plant cellulose synthase}, volume={65}, ISSN={1460-2431 0022-0957}, url={http://dx.doi.org/10.1093/jxb/eru383}, DOI={10.1093/jxb/eru383}, abstractNote={Summary Computational modelling of peptide structure, genetic complementation in Arabidopsis thaliana, and confocal microscopy provide evidence that a region between two transmembrane helices may adopt two predominant structural conformations that affect the function of plant cellulose synthase.}, number={22}, journal={Journal of Experimental Botany}, publisher={Oxford University Press (OUP)}, author={Slabaugh, Erin and Sethaphong, Latsavongsakda and Xiao, Chaowen and Amick, Joshua and Anderson, Charles T. and Haigler, Candace H. and Yingling, Yaroslava G.}, year={2014}, month={Sep}, pages={6645–6653} }