2022 journal article

Aligned peptoid-based macrodiscs for structural studies of membrane proteins by oriented-sample NMR

BIOPHYSICAL JOURNAL, 121(17), 3263–3270.

By: A. Galiakhmetov n, C. Davern n, R. Esteves n, E. Awosanya n, Q. Guthrie n, C. Proulx n, A. Nevzorov

MeSH headings : Glycine; Lipids; Magnetic Resonance Spectroscopy; Membrane Proteins / chemistry; Peptoids / chemistry
TL;DR: The NMR spectra measured for the membrane-bound form of Pf1 coat protein incorporated in this new lipid mimetics demonstrate a higher order parameter and uniform linewidths as compared to the conventional bicelles and peptide-based macrodiscs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 11, 2022

2021 journal article

H-1/C-13/N-15 triple-resonance experiments for structure determinaton of membrane proteins by oriented-sample NMR

SOLID STATE NUCLEAR MAGNETIC RESONANCE, 111.

By: J. Lapin n, E. Awosanya n, R. Esteves n & A. Nevzorov n

author keywords: NMR pulse sequence design; Triple-resonance experiments; Dipolar couplings; Separated local-field spectroscopy; ROULETTE; Membrane proteins; Structure determination; Heteronuclear correlations
MeSH headings : Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy / methods; Membrane Proteins / chemistry; Nuclear Magnetic Resonance, Biomolecular / methods; Protons
TL;DR: The presented developments advance NMR of oriented samples for structure determination of membrane proteins and liquid crystals in solid-state NMR experiments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: March 8, 2021

2020 journal article

NMR "Crystallography" for Uniformly (C-13, N-15)-Labeled Oriented Membrane Proteins

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 59(9), 3554–3557.

By: E. Awosanya n, J. Lapin n & A. Nevzorov n

author keywords: dipolar couplings; membrane proteins; nuclear magnetic resonance; oriented samples; structure determination
MeSH headings : Carbon Isotopes / chemistry; Inovirus / metabolism; Isotope Labeling; Lipid Bilayers / chemistry; Lipid Bilayers / metabolism; Membrane Proteins / chemistry; Nitrogen Isotopes / chemistry; Nuclear Magnetic Resonance, Biomolecular; Viral Proteins / chemistry
TL;DR: A new experimental triple-resonance NMR technique was applied to uniformly doubly labeled Pf1 coat protein in magnetically aligned DMPC/DHPC bicelles and yielded a consensus α-helical transmembrane structure for Pf1 protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: February 10, 2020

2018 journal article

Protein Rotational Dynamics in Aligned Lipid Membranes Probed by Anisotropic T-1 rho NMR Relaxation

BIOPHYSICAL JOURNAL, 114(2), 392–399.

By: E. Awosanya n & A. Nevzorov n

MeSH headings : Anisotropy; Cell Membrane / chemistry; Cell Membrane / metabolism; Diffusion; Dimyristoylphosphatidylcholine / chemistry; Magnetic Resonance Spectroscopy; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Micelles; Phospholipid Ethers / chemistry; Rotation
TL;DR: The method illustrates the use of single-helix transmembrane peptides as molecular probes to assess the dynamic parameters of biological membranes by NMR relaxation in oriented lipid bilayers. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Uniaxial Diffusional Narrowing of NMR Lineshapes for Membrane Proteins Reconstituted in Magnetically Aligned Bicelles and Macrodiscs

APPLIED MAGNETIC RESONANCE, 49(12), 1335–1353.

By: D. Tesch n, Z. Pourmoazzen n, E. Awosanya n & A. Nevzorov n

UN Sustainable Development Goal Categories
Source: Web Of Science
Added: December 17, 2018

2017 journal article

Sensitivity enhancement for membrane proteins reconstituted in parallel and perpendicular oriented bicelles obtained by using repetitive cross-polarization and membrane-incorporated free radicals

JOURNAL OF BIOMOLECULAR NMR, 67(2), 135–144.

By: S. Koroloff n, D. Tesch n, E. Awosanya n & A. Nevzorov n

author keywords: Oriented-sample NMR (OS-NMR); Sensitivity enhancement; Paramagnetic relaxation enhancement; Repetitive cross-polarization; Membrane proteins; Pf1; Spectroscopic assignment; Magnetically aligned bicelles
MeSH headings : Bacteriophage Pf1; Free Radicals / chemistry; Magnetic Resonance Spectroscopy / methods; Magnetic Resonance Spectroscopy / standards; Membrane Proteins / chemistry; Signal-To-Noise Ratio; Viral Proteins / chemistry
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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