Works (24)

Updated: July 5th, 2023 16:03

2015 article

Assessing Intermolecular RNA:RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping

RNA-RNA INTERACTIONS: METHODS AND PROTOCOLS, Vol. 1240, pp. 125–134.

By: K. Gagnon* & E. Maxwell n

author keywords: Intermolecular RNA:RNA base pairing; Lead cleavage mapping; RNP assembly; Gel electrophoresis; Non-coding RNA
MeSH headings : Base Pairing; Base Sequence; Lead / metabolism; Molecular Biology / methods; Molecular Sequence Data; Multiprotein Complexes / metabolism; Nucleic Acid Denaturation; RNA / genetics; RNA / metabolism; Ribonucleoproteins / metabolism; Saccharomyces cerevisiae / metabolism; Sequence Analysis, RNA
TL;DR: This chapter details how heavy metal cleavage mapping analysis was used to map the intermolecular RNA:RNA base pairing of a box C/D RNA with its target RNA within the assembling archaeal RNP complex. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Structurally Conserved Nop56/58 N-terminal Domain Facilitates Archaeal Box C/D Ribonucleoprotein-guided Methyltransferase Activity

JOURNAL OF BIOLOGICAL CHEMISTRY, 287(23), 19418–19428.

MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Chromosomal Proteins, Non-Histone / chemistry; Chromosomal Proteins, Non-Histone / genetics; Chromosomal Proteins, Non-Histone / metabolism; Methanococcales / chemistry; Methanococcales / genetics; Methanococcales / metabolism; Methyltransferases / chemistry; Methyltransferases / genetics; Methyltransferases / metabolism; Protein Binding; Protein Structure, Tertiary; Ribonucleoproteins / chemistry; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism
TL;DR: The archaeal Nop56/58 NTD exhibits a conserved domain structure whose exceptionally stable interaction with fibrillarin plays a role in both RNP assembly and methyltransferase activity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Comparative Analysis of the 15.5kD Box C/D snoRNP Core Protein in the Primitive Eukaryote Giardia lamblia Reveals Unique Structural and Functional Features

BIOCHEMISTRY, 50(14), 2907–2918.

MeSH headings : Amino Acid Sequence; Animals; Binding Sites / genetics; Circular Dichroism; Crystallography, X-Ray; Giardia lamblia / metabolism; Humans; Methyltransferases / chemistry; Methyltransferases / metabolism; Models, Molecular; Molecular Sequence Data; Molecular Weight; Phylogeny; Protein Binding; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Protozoan Proteins / chemistry; Protozoan Proteins / genetics; Protozoan Proteins / metabolism; RNA / genetics; RNA / metabolism; Ribonucleoproteins, Small Nucleolar / chemistry; Ribonucleoproteins, Small Nucleolar / classification; Ribonucleoproteins, Small Nucleolar / metabolism; Sequence Homology, Amino Acid; Temperature
TL;DR: Comp comparative sequence analysis indicated a distinct evolutionary position between Archaea and Eukarya, and the Giardia 15.5kD protein occupies a unique position in the evolution of this box C/D RNP core protein retaining structural and functional features characteristic of both archaeal L7Ae and higher eukaryotic 15. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

The Spatial-Functional Coupling of Box C/D and C '/D ' RNPs Is an Evolutionarily Conserved Feature of the Eukaryotic Box C/D snoRNP Nucleotide Modification Complex

MOLECULAR AND CELLULAR BIOLOGY, 31(2), 365–374.

By: G. Qu n, R. Nues*, N. Watkins* & E. Maxwell n

MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Base Sequence; Eukaryota / genetics; Evolution, Molecular; Fungal Proteins / genetics; Fungal Proteins / metabolism; Humans; Methylation; Molecular Sequence Data; Nucleic Acid Conformation; Nucleotides / genetics; Nucleotides / metabolism; RNA, Archaeal / genetics; RNA, Archaeal / metabolism; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Ribonucleoproteins / chemistry; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism; Ribonucleoproteins, Small Nucleolar / chemistry; Ribonucleoproteins, Small Nucleolar / genetics; Ribonucleoproteins, Small Nucleolar / metabolism
TL;DR: The spatial-functional coupling of box C/D and C′/D′ RNPs is an evolutionarily conserved feature of both archaeal and eukaryotic box C /D RNP complexes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Signature amino acids enable the archaeal L7Ae box C/D RNP core protein to recognize and bind the K-loop RNA motif

RNA, 16(1), 79–90.

author keywords: box C/D snoRNA; snoRNP; kink-turn; L7Ae/L30 proteins; RNA-protein interaction
MeSH headings : Amino Acid Motifs / physiology; Amino Acid Sequence; Archaea / genetics; Archaea / metabolism; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Base Sequence; Binding Sites / genetics; Conserved Sequence; Models, Biological; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Protein Binding / genetics; RNA, Archaeal / chemistry; RNA, Archaeal / metabolism; Ribonucleoproteins / chemistry; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism; Sequence Homology, Amino Acid
TL;DR: These five signature amino acids are distinct for each of the L7Ae/L30 family members, suggesting an evolutionary continuum of these RNA-binding proteins for recognition of the various K-turn motifs contained in their cognate RNAs. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Structural Basis for Substrate Placement by an Archaeal Box C/D Ribonucleoprotein Particle

MOLECULAR CELL, 39(6), 939–949.

By: S. Xue*, R. Wang*, F. Yang*, R. Terns*, M. Terns*, X. Zhang n, E. Maxwel n, H. Li*

MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Binding Sites / physiology; Biocatalysis; Chromosomal Proteins, Non-Histone / chemistry; Chromosomal Proteins, Non-Histone / genetics; Chromosomal Proteins, Non-Histone / metabolism; Crystallography, X-Ray; Holoenzymes / chemistry; Holoenzymes / genetics; Holoenzymes / metabolism; Methyltransferases / chemistry; Methyltransferases / genetics; Methyltransferases / metabolism; Models, Molecular; Mutagenesis, Insertional / physiology; Nuclear Proteins / chemistry; Nuclear Proteins / genetics; Nucleic Acid Conformation; Protein Multimerization / physiology; Protein Structure, Quaternary / physiology; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; RNA, Archaeal / chemistry; RNA, Archaeal / metabolism; RNA, Small Nucleolar / chemistry; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Ribonucleoproteins, Small Nucleolar / chemistry; Ribonucleoproteins, Small Nucleolar / genetics; Ribonucleoproteins, Small Nucleolar / metabolism; Sequence Deletion / physiology
TL;DR: The predicted asymmetric nature of the holoenzyme is consistent with previous biochemical data on RNP assembly and provides a simple solution for accommodating base-pairing between the C/D guide RNA and large ribosomal and spliceosomal substrate RNAs. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

A Dimeric Structure for Archaeal Box C/D Small Ribonucleoproteins

SCIENCE, 325(5946), 1384–1387.

MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / metabolism; Archaeal Proteins / ultrastructure; Base Sequence; Chromosomal Proteins, Non-Histone / chemistry; Methanococcales / chemistry; Microscopy, Electron; Models, Molecular; Molecular Weight; Nucleic Acid Conformation; Protein Multimerization; Protein Structure, Quaternary; Protein Structure, Tertiary; RNA, Archaeal / chemistry; RNA, Archaeal / ultrastructure; Ribonucleoproteins / chemistry; Ribonucleoproteins / metabolism; Ribonucleoproteins / ultrastructure
TL;DR: The structure of a reconstituted and catalytically active box C/D sRNP from the archaeon Methanocaldococcus jannaschii was determined by single-particle electron microscopy and Mutational analysis demonstrated that this di-sRNP structure was relevant for the enzymatic function of archaeal box C /D sRNPs. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

A role for hydrophobicity in a DielsAlder reaction catalyzed by pyridyl-modified RNA

NUCLEIC ACIDS RESEARCH, 37(9), 3074–3082.

By: K. Gagnon n, S. Ju n, M. Goshe n, E. Maxwell n & S. Franzen n

MeSH headings : Anthracenes / metabolism; Base Sequence; Catalysis; Ethylene Glycols / metabolism; Hydrophobic and Hydrophilic Interactions; Lactones; Molecular Sequence Data; Nucleic Acid Conformation; Pyridines / chemistry; RNA / chemistry; RNA / metabolism; RNA, Catalytic / chemistry; RNA, Catalytic / metabolism; Sesquiterpenes
TL;DR: It is shown that DA_TR96, the most active member of the DA22 RNA sequence family, which was selected with pyridyl-modified nucleotides, accelerates a cycloaddition reaction between anthracene and maleimide derivatives with high turnover, and suggests a key role for the hydrophobic effect in the catalytic mechanism. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

Xenopus microRNA genes are predominantly located within introns and are diffferentially expressed in adult frog tissues via post-transcriptional regulation

GENOME RESEARCH, 18(1), 104–112.

By: G. Tang n & E. Maxwell n

MeSH headings : Animals; Genome / physiology; Humans; Introns / physiology; MicroRNAs / biosynthesis; MicroRNAs / genetics; Organ Specificity / physiology; RNA Precursors / biosynthesis; RNA Precursors / genetics; RNA Processing, Post-Transcriptional / physiology; Xenopus laevis
TL;DR: Analysis of Xenopus tropicalis miRNAs indicated that post-transcriptional processing plays an important role in regulating miRNA expression in the amphibian Xenopus. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 review

In vitro reconstitution and affinity purification of catalytically active archaeal box C/D sRNP complexes

[Review of ]. RNA modification, 425, 263–282.

By: K. Gagnon n, X. Zhang n & E. Maxwell n

MeSH headings : Chromatography, Affinity / methods; Chromatography, Ion Exchange / methods; Methanococcaceae; Ribonucleoproteins, Small Nuclear / isolation & purification
TL;DR: A novel affinity purification scheme that uses differentially tagged core proteins and a sequential three-step affinity selection protocol that yields fully assembled and catalytically active box C/D RNPs. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Structural features of the guide : target RNA duplex required for archaeal box C/D sRNA-guided nucleotide 2 '-O-methylation

RNA, 13(6), 899–911.

By: C. Appel n & E. Maxwell

author keywords: box C/D RNA; sRNA; snoRNA; RNA; guided nucleotide modification; nucleotide methylation
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Base Pairing; Base Sequence; DNA Primers / genetics; Macromolecular Substances / chemistry; Macromolecular Substances / metabolism; Magnesium / metabolism; Methanococcales / genetics; Methanococcales / metabolism; Methylation; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Nucleotides / chemistry; Nucleotides / metabolism; RNA, Archaeal / chemistry; RNA, Archaeal / genetics; RNA, Archaeal / metabolism; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; Ribonucleoproteins / chemistry; Ribonucleoproteins / genetics; Ribonucleoproteins / metabolism
TL;DR: Interestingly, D and D' target RNAs exhibited different levels of methylation when deoxynucleotides were inserted into the target RNA or when target methylation was carried out in elevated Mg(2+) concentrations, which suggested that unique structural features of the box C/D and C'/D' RNPs differentially affect their respective methylation capabilities. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Assembly of the archaeal box C/D sRNP can occur via alternative pathways and requires temperature-facilitated sRNA remodeling

Journal of Molecular Biology, 362(5), 1025–1042.

By: K. Gagnon n, X. Zhang n, P. Agris n & E. Maxwell n

MeSH headings : Amino Acid Sequence; Archaea / chemistry; Archaea / genetics; Archaea / metabolism; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; Base Sequence; Chromatography, Affinity; Circular Dichroism; Methylation; Models, Biological; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Protein Binding; RNA, Archaeal / chemistry; RNA, Archaeal / genetics; RNA, Archaeal / metabolism; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; RNA, Guide, Kinetoplastida / metabolism; RNA, Small Nucleolar / chemistry; RNA, Small Nucleolar / genetics; RNA, Small Nucleolar / metabolism; Ribonucleoproteins, Small Nuclear / chemistry; Ribonucleoproteins, Small Nuclear / genetics; Ribonucleoproteins, Small Nuclear / metabolism; Temperature
TL;DR: RNA remodeling appears to be a common and requisite feature of archaeal dual-guide box C/D sR NP assembly and formation of the mature sRNP can follow different assembly pathways in generating catalytically active complexes. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Conserved spacing between the box C/D and C '/D ' RNPs of the archaeal box C/D sRNP complex is required for efficient 2 '-O-methylation of target RNAs

RNA, 11(3), 285–293.

By: E. Tran n, . Zhang n, L. Lackey n & E. Maxwell n

author keywords: archaeal sRNA; box C/D RNA; box C/D RNP; snoRNA; nucleotide modification
MeSH headings : Archaeal Proteins / metabolism; Base Sequence; DNA Primers; Methylation; Nucleic Acid Conformation; RNA, Archaeal / chemistry; RNA, Archaeal / metabolism; Ribonucleoproteins / metabolism
TL;DR: The importance of spatially positioning the box C/D and C'/D' RNPs within the sRNP complex for nucleotide modification is investigated for preservation of critical inter-RNP interactions required for efficient box C /D sR NP-guided nucleotide methylation. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs

BIOCHEMISTRY, 44(28), 9657–9672.

By: J. Suryadi n, E. Tran n, E. Maxwell n & B. Brown n

MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Archaeal Proteins / chemistry; Archaeal Proteins / metabolism; Base Sequence; Crystallography, X-Ray; Methanococcus; Models, Molecular; Molecular Sequence Data; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Secondary; RNA, Archaeal / chemistry; RNA, Archaeal / metabolism; RNA-Binding Proteins / chemistry; RNA-Binding Proteins / metabolism; Ribonucleoproteins, Small Nuclear / chemistry; Ribonucleoproteins, Small Nuclear / metabolism; Ribosomal Proteins / chemistry; Ribosomal Proteins / metabolism; Structural Homology, Protein; Thermodynamics
TL;DR: Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif, suggesting that RNA binding occurs through an induced-fit interaction. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Evolutionary origins of the RNA-guided nucleotide-modification complexes: from the primitive translation apparatus?

Trends in Biochemical Sciences, 29(7), 343–350.

By: E. Tran n, J. Brown n & E. Maxwell n

MeSH headings : Amino Acid Sequence; Conserved Sequence; DNA Methylation; Evolution, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Protein Biosynthesis; Protein Conformation; RNA / genetics; RNA / physiology; Ribonucleoproteins / chemistry; Ribonucleoproteins / genetics; Ribonucleotides / metabolism; Ribosomes / chemistry; Ribosomes / genetics
TL;DR: The trans -acting, RNA-guided nucleotide-modification complexes of the modern RNP world seem to have evolved from cis -acting RNA or RNP elements contained in the primitive translation apparatus during the transition from the ancient RNA world to the modernRNP world. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2004 journal article

Sequential 2 '-O-methylation of archaeal pre-tRNA(Trp) nucleotides is guided by the intron-encoded but trans-acting box c/D ribonucleoprotein of pre-tRNA

JOURNAL OF BIOLOGICAL CHEMISTRY, 279(46), 47661–47671.

By: K. Sanjay*, S. Singh, P. Gurha*, E. Tran n, E. Maxwell n & R. Gupta*

MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Gene Expression Regulation, Archaeal; Haloferax volcanii / genetics; Haloferax volcanii / metabolism; Introns; Methylation; Mutagenesis, Site-Directed; Nucleic Acid Conformation; RNA Precursors / chemistry; RNA Precursors / metabolism; RNA, Archaeal / chemistry; RNA, Archaeal / metabolism; RNA, Transfer, Trp / chemistry; RNA, Transfer, Trp / metabolism; Ribonucleoproteins, Small Nuclear / chemistry; Ribonucleoproteins, Small Nuclear / genetics; Ribonucleoproteins, Small Nuclear / metabolism
TL;DR: It is demonstrated that pre-tRNA methylation is guided in trans by the intron-encoded box C/D RNPs positioned in either another pre-TRNATrp or in the excised intron, which could explain the observed accumulation of the excising H. volcanii pre- tR NATrp intron in vivo. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Efficient RNA 2 '-O-methylation requires juxtaposed and symmetrically assembled archaeal box C/D and C '/D ' RNPs

EMBO JOURNAL, 22(15), 3930–3940.

By: E. Tran n, . Zhang & E. Maxwell

author keywords: Archaea; box C; D RNP; ribonucleotide methylation; snoRNA; sRNA
MeSH headings : Archaea / metabolism; Archaeal Proteins / chemistry; Archaeal Proteins / metabolism; Base Sequence; Methylation; Molecular Sequence Data; Nucleic Acid Conformation; Protein Binding; RNA, Archaeal / chemistry; RNA, Archaeal / metabolism; Ribonucleoproteins / metabolism
TL;DR: In vitro assembly of an archaeal box C/D sRNP using recombinant core proteins L7, Nop56/58 and fibrillarin has yielded an RNA:protein enzyme that guides methylation from both the terminal box C-D core and internal C′/D′ RNP complexes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Archaeal ribosomal protein L7 is a functional homolog of the eukaryotic 15.5kD/Snu13p snoRNP core protein

Nucleic Acids Research, 30(4), 931–941.

By: J. Kuhn n, E. Tran & E. Maxwell

MeSH headings : Amino Acid Sequence; Archaeal Proteins / genetics; Archaeal Proteins / physiology; Binding Sites; Electrophoretic Mobility Shift Assay; Escherichia coli / genetics; Escherichia coli Proteins; Eukaryotic Cells / metabolism; Humans; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Nucleic Acid Denaturation; RNA, Small Nucleolar / chemistry; RNA, Small Nucleolar / metabolism; Recombinant Proteins / metabolism; Ribonucleoproteins, Small Nuclear / genetics; Ribosomal Proteins / genetics; Ribosomal Proteins / physiology; Saccharomyces cerevisiae Proteins / genetics; Sequence Homology, Amino Acid
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

A well-connected and conserved nucleoplasmic helicase is required for production of box C/D and H/ACA snoRNAs and localization of snoRNP proteins

MOLECULAR AND CELLULAR BIOLOGY, 21(22), 7731–7746.

By: T. King*, W. Decatur*, E. Bertrand*, E. Maxwell n & M. Fournier*

MeSH headings : Adenosine Triphosphatases / genetics; Adenosine Triphosphatases / metabolism; Amino Acid Motifs; Animals; COS Cells; Cell Nucleus / metabolism; Chlorocebus aethiops; Conserved Sequence; DNA Helicases; Fungal Proteins / genetics; Fungal Proteins / metabolism; Mice; Nuclear Proteins / metabolism; RNA Helicases / genetics; RNA Helicases / metabolism; RNA Precursors / metabolism; RNA Processing, Post-Transcriptional; RNA, Small Nucleolar / metabolism; Ribonucleoproteins, Small Nucleolar / metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
TL;DR: It is reported that theSaccharomyces cerevisiae ortholog of p50 (Rvb2, Tih2p, and other names) is required for production of C/D snoRNAs in vivo and, surprisingly, H/ACA sno RNAs as well and may play a role in coupling snoRNA synthesis with snoRNP assembly and localization. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Box C/D snoRNA-associated proteins: Two pairs of evolutionarily ancient proteins and possible links to replication and transcription

RNA, 6(6), 861–879.

By: D. Newman n, J. Kuhn n, G. Shanab* & E. Maxwell n

author keywords: intronic snoRNA; snoRNA processing; snoRNP proteins
MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Animals; Conserved Sequence; DNA Replication; Evolution, Molecular; Mice; Molecular Sequence Data; Nuclear Proteins; RNA, Small Nucleolar / genetics; RNA, Small Nucleolar / physiology; Ribonucleoproteins, Small Nucleolar / genetics; Ribonucleoproteins, Small Nucleolar / physiology; Saccharomyces cerevisiae Proteins; Sequence Alignment; Substrate Specificity; Transcription, Genetic
TL;DR: Homologs for both snoRNA-associated protein pairs occur in Archaea, strengthening the hypothesis that the box C/D RNA elements and their interacting proteins are of ancient evolutionary origin. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs

EMBO JOURNAL, 17(11), 3176–3187.

By: T. Lange, A. Borovjagin, E. Maxwell & S. Gerbi

author keywords: Boxes C and D; nucleolar localization; snoRNA
MeSH headings : Animals; Base Sequence; Binding, Competitive / genetics; Cell Nucleolus / chemistry; Cell Nucleolus / genetics; Conserved Sequence / genetics; Fluorescein / metabolism; HSP70 Heat-Shock Proteins / genetics; Introns; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Localization Signals / genetics; Oligonucleotides / metabolism; RNA Processing, Post-Transcriptional; RNA, Ribosomal / metabolism; RNA, Small Nuclear / chemistry; RNA, Small Nuclear / metabolism; Xenopus laevis
TL;DR: U14 snoRNA is shown to be required for 18S rRNA production in vertebrates and Mutations in Box C/D of U8 sno RNA also impaired nucleolar localization, suggesting the general importance of Boxes C and D as nucleolar localized sequences for Box C-D snoRNAs. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein

RNA, 4(5), 582–593.

By: N. Watkins n, D. Newman n, J. Kuhn n & E. Maxwell n

author keywords: nucleolus; RNA processing, RNA-protein interaction; snoRNA
MeSH headings : Animals; Ascitic Fluid / cytology; Cell Extracts; Cross-Linking Reagents; HeLa Cells; Humans; Mice; Molecular Weight; Nucleic Acid Conformation; Oocytes; Protein Binding; RNA, Small Nuclear / chemistry; RNA, Small Nuclear / genetics; RNA, Small Nuclear / metabolism; RNA-Binding Proteins / chemistry; RNA-Binding Proteins / isolation & purification; RNA-Binding Proteins / metabolism; Ribonucleoproteins, Small Nuclear / biosynthesis; Ribonucleoproteins, Small Nuclear / chemistry; Ultraviolet Rays; Xenopus
TL;DR: An in vitro U14 snoRNP assembly system is established and electrophoretic mobility-shift analysis demonstrated that all the sequences and structures of the box C/D core motif required for U14 processing are also necessary for protein binding and sno RNP assembly. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Processing of vertebrate box C/D small nucleolar RNAs in plant cells

EUROPEAN JOURNAL OF BIOCHEMISTRY, 253(1), 154–160.

By: D. Leader, G. Clark, J. Boag, J. Watters, C. Simpson, N. Watkins*, E. Maxwell n, J. Brown

author keywords: small nucleolar RNA; box C/D small nucleolar RNA; small nucleolar RNA processing; U14
MeSH headings : Animals; Base Sequence; Female; Humans; In Vitro Techniques; Introns; Mice; Oligodeoxyribonucleotides / genetics; Oocytes / metabolism; Plants, Genetically Modified; Plants, Toxic; RNA Processing, Post-Transcriptional; RNA Splicing; RNA, Small Nuclear / chemistry; RNA, Small Nuclear / genetics; RNA, Small Nuclear / metabolism; Tobacco / genetics; Tobacco / metabolism; Xenopus
TL;DR: The similarity of processing behaviour of the vertebrate box C/D constructs in tobacco protoplasts and Xenopus oocytes suggests the mechanism of processing, involving recognition and association of proteins, is conserved in plants. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1997 journal article

Identification of specific nucleotide sequences and structural elements required for intronic U14 snorna processing

RNA, 3(1), 17–26.

By: L. Xia, N. Watkins & E. Maxwell

Source: NC State University Libraries
Added: August 6, 2018

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