@article{silva_martins_sponchiado_rocha_pohler_peñagaricano_binelli_2023, title={Hormonal profile prior to luteolysis modulates the uterine luminal transcriptome in the subsequent cycle in beef cross-bred cows}, volume={108}, url={http://dx.doi.org/10.1093/biolre/ioad035}, DOI={10.1093/biolre/ioad035}, abstractNote={Abstract Sex steroid concentrations modulate endometrial function and fertility in cattle. Our objective was to compare the post-estrus luminal transcriptome of cows that were exposed to contrasting concentrations of progesterone (P4) before luteolysis that displayed estrus and ovulated spontaneously. Cross-bred beef cows received either (1) a new CIDR and GnRH (day -9; high progesterone treatment; HP4; n = 16) or (2) a previously used CIDR, PGF2α, and GnRH (low progesterone treatment; LP4; n = 24). All cows received PGF2α at CIDR removal (day -2). Ovarian ultrasonography and blood collections were performed on days -9, -2, -0.5, and 0 (day of observed estrus), and days 4, 7, and 14 for measurement of ovarian structures, P4, and estradiol (E2). Luminal epithelial cells were collected using a cytology brush on days 4, 7, and 14 for RNAseq. On day -2, CL area and concentrations of P4 were greater, while on day -0.5, concentrations of E2 were decreased in HP4. Ovarian structures and hormonal concentrations were similar on days 4, 7, or 14 (P > 0.05). There were enriched pathways in HP4 related to activation and signaling of the innate immune system at day 4, downregulation in the network involved in the extracellular matrix remodeling at day 7, and exacerbated inflammatory response as well as differentiation and activation of macrophages at day 14 (Benjamini–Hochberg P-value ≤ 0.05). In conclusion, manipulation of pre-luteolysis sex steroid concentrations altered the post-estrus luminal transcriptome even though all cows showed estrus and ovulated spontaneously. Summary Sentence The pre-luteolysis hormonal profile altered the luminal transcriptome on days 4, 7, and 14 post-estrus in beef cross-bred cows. Graphical Abstract}, number={6}, journal={Biology of Reproduction}, publisher={Oxford University Press (OUP)}, author={Silva, Felipe A C C and Martins, Thiago and Sponchiado, Mariana and Rocha, Cecilia C and Pohler, Ky G and Peñagaricano, Francisco and Binelli, Mario}, year={2023}, month={Jun}, pages={922–935} }
 @article{silva_martins_sponchiado_rocha_ashrafi_graham_pohler_peñagaricano_gonella-diaza_binelli_2023, title={Pre-estrus progesterone does not affect post-estrus luminal metabolome in cross-bred beef cows}, volume={166}, ISSN={1470-1626 1741-7899}, url={http://dx.doi.org/10.1530/REP-22-0372}, DOI={10.1530/REP-22-0372}, abstractNote={In cattle, the concentration of sex steroids modulates uterine function, which is reflected in the composition of the luminal metabolome. Ultimately, the uterine luminal metabolome influences embryonic growth and development. Our objectives were (1) to compare the luminal metabolome 4, 7, and 14 days after estrus of cows that were exposed to greater (HP4; n = 16) vs. lower (LP4; n = 24) concentrations of progesterone before displaying estrus and ovulating spontaneously and (2) to identify changes in the luminal concentration of metabolites across these time points. Luminal epithelial cells and fluid were collected using a cytology brush and gene expression and metabolite concentrations were assessed by RNAseq and targeted mass spectrometry, respectively. Metabolome profile was similar between treatments within each of days 4, 7, and 14 (FDR ≥ 0.1). Concentrations of 53 metabolites changed, independent of treatment, across the diestrus. Metabolites were mostly lipids (40 out 53) and the greatest concentrations were at d 14 (FDR ≤ 0.1). On d 7, the concentration of putrescine and the gene expression of ODC1, PAOX, SLC3A2, and SAT1 increased (P ≤ 0.05). On d 14, the concentration of three ceramides, four glucosylceramides, and 12 sphingomyelins and the expression of SGMS2 were increased, in addition to the concentration of choline and 20 phosphatidylcholines. Collectively, the post-estrus concentration of luminal metabolites changed dynamically, independent of the concentration of sex steroids on the previous cycle, and the greatest magnitude changes were on day 14, when lipid metabolism was the most enriched pathway.}, number={2}, journal={Reproduction}, publisher={Bioscientifica}, author={Silva, Felipe A C C and Martins, Thiago and Sponchiado, Mariana and Rocha, Cecilia C and Ashrafi, Nadia and Graham, Stewart F and Pohler, Ky G and Peñagaricano, Francisco and Gonella-Diaza, Angela M and Binelli, Mario}, year={2023}, month={May}, pages={99–116} }
 @article{rocha_martins_silva_sponchiado_pohler_binelli_2023, title={Viperin (RSAD2) gene expression in peripheral blood mononuclear cells of pregnant crossbred beef cows is altered by Bos indicus genetics}, volume={209}, ISSN={["1879-3231"]}, DOI={10.1016/j.theriogenology.2023.07.003}, abstractNote={The expression of interferon (IFN) stimulated genes (ISGs) in lymphocytes has been used for pregnancy diagnosis in cattle. However, among-cow variability has yielded sub-optimal predictive accuracy. We hypothesized that the expression of ISGs (ISG15, OAS1, RSAD2, CLEC3B, and AKR1B1) in early pregnancy varies according to the proportion of Bos indicus (B. indicus) genetics on females. Multiparous cows were classified in three genetic groups, High Angus (HA; n = 45 [0–33% Brahman influence]), Angus-Brahman (AB; n = 30 [34–67%]), and High Brahman (HB; n = 19 [68–100%]) and submitted to a Select-Synch + CIDR protocol. Cows that displayed estrus (n = 94) were artificially inseminated (Day0; D0). On D19, blood samples were collected to obtain peripheral blood mononuclear cells (PBMC) and measure progesterone (P4) concentrations. On D30, pregnancy diagnosis was performed. The expression of RSAD2 in PBMC of pregnant cows was positively related to the proportion of B. indicus genetics of the groups, but not the expression of ISG15 and OAS1. In pregnant cows, the proportion of B. indicus genetics was negatively associated to circulating levels of P4 concentrations. The P4 concentrations were related positively with RSAD2 expression. ROC curve results determined that for cattle with B. indicus genetics lower than 67%, the CLEC3B and AKR1B1 combination was the most accurate option to predict the outcome of pregnancy. In cows with more than 68% of B. indicus genetics, RSAD2 provided the best accuracy. In conclusion, there is a relationship between the proportion of B. indicus genetics and the ISGs gene expression in PBMC during pregnancy.}, journal={THERIOGENOLOGY}, author={Rocha, Cecilia Constantino and Martins, Thiago and Silva, Felipe Alves Correa Carvalho and Sponchiado, Mariana and Pohler, Ky Garrett and Binelli, Mario}, year={2023}, month={Oct}, pages={226–233} }
 @article{binelli_gonella-diaza_martins_rocha_silva_tarnonsky_roskopf_rae_driver_dilorenzo_et al._2022, title={Calculating Calf Performance in Beef Operations: The University of Florida Beef Herds in the 2019–2020 Calving Season}, volume={2022}, url={https://doi.org/10.32473/edis-an384-2022}, DOI={10.32473/edis-an384-2022}, abstractNote={This article aims to report the calf performance of two beef herds of the University of Florida, located in north (UF/IFAS NFREC, Marianna) and central Florida (Beef Units, Gainesville). Written by Mario Binelli, Angela M. Gonella-Diaza, Thiago Martins, Cecilia C. Rocha, Felipe A. C. C. Silva, Federico Tarnonsky, Sergio Roskopf, Owen Rae, Danny Driver, Nicolas DiLorenzo, Jose Dubeux, and David Thomas, and published by the UF/IFAS Department of Animal Sciences, February 2022.}, number={1}, journal={EDIS}, publisher={University of Florida George A Smathers Libraries}, author={Binelli, Mario and Gonella-Diaza, Angela Maria and Martins, Thiago and Rocha, Cecilia Constantino and Silva, Felipe Alves Correa Carvalho and Tarnonsky, Federico and Roskopf, Sergio Daniel and Rae, Owen and Driver, Joseph Daniel and DiLorenzo, Nicolas and et al.}, year={2022}, month={Feb} }
 @article{rocha_silva_martins_marrero_bromfield_driver_hansen_oliveira_binelli_2022, title={Culture of endometrial epithelial cells collected by a cytological brush in vivo}, volume={3}, url={https://doi.org/10.3168/jdsc.2021-0189}, DOI={10.3168/jdsc.2021-0189}, abstractNote={Graphical Abstract Summary: Understanding endometrial physiology in dairy cows is essential, as approximately 50% of pregnancies are lost in the first 3 weeks of gestation for unknown reasons. This work validated an approach to culture and propagate luminal endometrial cells harvest by a minimally invasive tool, endometrial cytology (cytobrush). (1) Cells were collected at a specific stage of the estrous cycle; (2) fresh cells were evaluated regarding their identity by immunofluorescence; and (3) functionality of the system was demonstrated by inducing transcription of IFN-stimulated genes (ISGs) in response to treatment with recombinant IFN-τ.}, number={3}, journal={JDS Communications}, publisher={American Dairy Science Association}, author={Rocha, Cecilia Constantino and Silva, Felipe Alves Correa and Martins, Thiago and Marrero, Marcela G. and Bromfield, John J. and Driver, John and Hansen, Thomas and Oliveira, Lilian and Binelli, Mario}, year={2022}, month={May}, pages={217–221} }
 @article{silva_camacho_lemley_hallford_swanson_vonnahme_2022, title={Effects of nutrient restriction and subsequent realimentation in pregnant beef cows: Maternal endocrine profile, umbilical hemodynamics, and mammary gland development and hemodynamics}, volume={191}, ISSN={0093-691X}, url={http://dx.doi.org/10.1016/j.theriogenology.2022.08.009}, DOI={10.1016/j.theriogenology.2022.08.009}, abstractNote={Our hypothesis was that maternal nutrient restriction would negatively impact the endocrine and metabolic status of the pregnant cow, therefore influencing the mammary gland in preparation for lactation. We further hypothesized that earlier timing of realimentation could prevent negative impacts of nutrient restriction. The objectives were to investigate the influence of nutrient restriction and realimentation during early to late gestation on endocrine profile, umbilical hemodynamics, and mammary gland development and hemodynamics in pregnant beef cows. In Experiment 1, on d 30 of pregnancy cows (initial BW = 667.5 ± 13.4 kg, BCS = 6.2 ± 0.1) were randomly assigned to one of 3 treatments: 1) 100% NRC requirements from d 30 to 254 of gestation (CCC; n = 6); 2) 60% NRC from d 30 to 85, thereafter being re-alimented to 100% NRC to d 254 (RCC; n = 5); 3) or receive 60% NRC from d 30 to 140, thereafter being re-alimented to 100% NRC to d 254 (RRC; n = 6). Cows were returned to a common outdoor facility for calving thereafter and were fed ad libitum. In Experiment 2, on d 30 of pregnancy, cows (initial BW = 620.5 ± 11.3 kg, BCS = 5.1 ± 0.1) were randomly assigned to dietary treatments including: control (CON; 100% NRC; n = 18) and nutrient restriction (RES; 60% NRC; n = 30). On d 85 of pregnancy, cows were either slaughtered (CON, n = 6 and RES, n = 6), remained on control (CC; n = 12) and restricted (RR; n = 12) treatments, or were realimented to control (RC; n = 11). On d 140 of pregnancy, cows were either slaughtered (CC, n = 6; RR, n = 6; RC, n = 5), remained on control (CCC, n = 6; RCC, n = 5), or were realimented to control (RRC, n = 6). On d 254 of pregnancy, all remaining cows were slaughtered (CCC, n = 6; RCC, n = 5; RRC, n = 6). Mammary hemodynamics and endocrine profile were measured. Serum urea nitrogen, NEFA, as well as fetal parameters were measured in Experiment 1; whereas in Experiment 2, mammary gland development was recorded. In Experiment 1, RRC cows had lower dry matter intake (P = 0.001) and consequently lower BW change (P = 0.06). However, maternal nutrition did not alter mammary hemodynamics, hormonal patterns, and fetal characteristics (P > 0.11). In Experiment 2, CCC cows had increased (P = 0.02) mammary gland blood flow ipsilateral to the gravid horn as well as greater (P = 0.02) mammary gland fat on d 254. Nevertheless, plane of nutrition did not alter hormonal concentrations nor mammary gland characteristics (P > 0.15). These data indicate that nutrient restriction did not alter mammary hemodynamics nor endocrine profile throughout gestation.}, journal={Theriogenology}, publisher={Elsevier BV}, author={Silva, F.A.C.C. and Camacho, L.E. and Lemley, C.O. and Hallford, D.M. and Swanson, K.C. and Vonnahme, K.A.}, year={2022}, month={Oct}, pages={109–121} }
 @article{binelli_silva_rocha_martins_sponchiado_hoeck_cordeiro_campbell_leroy_peñagaricano_et al._2022, title={Endometrial receptivity in cattle: the mutual reprogramming paradigm}, url={https://doi.org/10.1590/1984-3143-ar2022-0097}, DOI={10.1590/1984-3143-ar2022-0097}, abstractNote={Abstract Prior to implantation in cattle, the mucous medium contained in the uterine lumen serves as a working interface for molecular exchange and signaling between the lining endometrium and the embryo. The composition of this luminal fluid changes temporally according to the secretory and reabsorptive activities of the uterus and the embryo, which are under complex regulation. Via this interface, both the embryo and the endometrium reprogram each other’s functions to support pregnancy continuation beyond the pre-implantation period. More specifically, the embryo receives elongation signals and the uterus receives anti-luteolytic stimuli. Here, characteristics of the luminal compartment as well as the regulation of its composition to determine the pregnancy outcome will be discussed.}, journal={Animal Reproduction}, author={Binelli, Mario and Silva, Felipe Alves Correa Carvalho and Rocha, Cecilia Constantino and Martins, Thiago and Sponchiado, Mariana and Hoeck, Veerle Van and Cordeiro, Andrey and Campbell, Meghan and Leroy, Jo L. M. R. and Peñagaricano, Francisco and et al.}, year={2022} }
 @book{binelli_gonella-diaza_martins_rocha_silva_rae_driver_dilorenzo_dubeux_thomas_2022, place={Gainesville, Florida}, title={Florida Yearling Beef Heifer Reproductive Performance Benchmarks}, institution={Panhandle Agriculture UF/IFAS Extension}, author={Binelli, M. and Gonella-Diaza, A.M. and Martins, T. and Rocha, C.C. and Silva, F.A.C.C. and Rae, O. and Driver, J.D. and DiLorenzo, N. and Dubeux, J. and Thomas, D.}, year={2022} }
 @book{binelli_gonella-diaza_martins_rocha_silva_rae_driver_dilorenzo_dubeux_thomas_2022, title={Florida Yearling Beef Heifer Reproductive Performance Benchmarks}, publisher={Panhandle Agriculture UF/IFAS Extension}, author={Binelli, M. and Gonella-Diaza, A.M. and Martins, T. and Rocha, C.C. and Silva, F.A.C.C. and Rae, O. and Driver, J.D. and DiLorenzo, N. and Dubeux, J. and Thomas, D.}, year={2022} }
 @inproceedings{silva_martins_sponchiado_pohler_penagaricano_binelli_2022, title={Increased concentration of progesterone prior to luteolysis increases cellular immune response and branched-chain amino acids degradation in the uterus during the subsequent luteal phase in beef cows}, booktitle={19th International Congress on Animal Reproduction (ICAR)}, author={Silva, F.A.C.C. and Martins, T. and Sponchiado, M. and Pohler, K.G. and Penagaricano, F. and Binelli, M.}, year={2022} }
 @article{martins_sponchiado_silva_estrada-cortés_hansen_peñagaricano_binelli_2022, title={Progesterone-dependent and progesterone-independent modulation of luminal epithelial transcription to support pregnancy in cattle}, volume={54}, ISSN={1094-8341 1531-2267}, url={http://dx.doi.org/10.1152/physiolgenomics.00108.2021}, DOI={10.1152/physiolgenomics.00108.2021}, abstractNote={In cattle, starting 4-5 days after estrus, pre-implantation embryonic development occurs in the confinement of the uterine lumen. Cells in the endometrial epithelial layer control the molecular traffic to and from the lumen and, thereby determine luminal composition. Starting early post-estrus, endometrial function is regulated by sex-steroids, but the effects of progesterone on luminal cells transcription have not been measured in vivo. First objective was to determine the extent to which progesterone controls transcription in luminal epithelial cells 4 d (D4) after estrus. Second objective was to discover luminal transcripts that predict pregnancy outcomes, when the effect of progesterone is controlled. Endometrial luminal epithelial cells were collected from embryo transfer recipients on D4 using a cytological brush and their transcriptome determined by RNASeq. Pregnancy by embryo transfer was measured on D30 (25 pregnant and 18 non-pregnant). Progesterone concentration on D4 was associated positively (n= 182) and negatively (n= 58) with gene expression. Progesterone-modulated transcription indicated an increase in oxidative phosphorylation, biosynthetic activity and proliferation of epithelial cells. When these effects of progesterone were controlled, different genes affected positively (n= 22) and negatively (n= 292) odds of pregnancy. These set of genes indicated that a receptive uterine environment was characterized by the inhibition of phosphoinositide signaling and innate immune system responses. A panel of 25 genes predicted the pregnancy outcome with sensitivity and specificity ranging from 64-96% and 44-83%, respectively. In conclusion, in the early diestrus, both progesterone-dependent and -independent mechanisms regulate luminal epithelial transcription associated with pregnancy outcomes in cattle.}, number={2}, journal={Physiological Genomics}, publisher={American Physiological Society}, author={Martins, Thiago and Sponchiado, Mariana and Silva, Felipe A. C. C. and Estrada-Cortés, Eliab and Hansen, Peter J. and Peñagaricano, Francisco and Binelli, Mario}, year={2022}, month={Feb}, pages={71–85} }
 @inproceedings{silva_silva_vieira_neto_junior_silva_nogueira_pugliesi_binelli_2021, title={49 The dynamic changes in uterine morphology and function during the proestrus-diestrus transition in beef cattle}, volume={33}, url={http://dx.doi.org/10.1071/rdv33n2ab49}, DOI={10.1071/rdv33n2ab49}, abstractNote={In cattle, early development of the non-attaching embryo depends exclusively on the biochemical properties of the intrauterine milieu. As the embryo transitions from the oviduct to the tip of the uterine horn around 4 days after oestrus, little is known about uterine functions that modulate the milieu composition and potentially determine pregnancy success. The objective was to characterise changes in uterine luminal fluid (ULF) accumulation and absorption, endometrial vascularity, and endometrial thickness in response to progesterone fluctuations during the peri-ovulatory period in beef heifers. Fourteen Bos indicus heifers that presented a prostaglandin F2α (PGF2α) responsive CL received 500 µg of PGF2α analogue i.m. (PGF) and were examined daily by rectal B-mode and pulse-wave colour-Doppler ultrasonography until the fifth day after oestrus (oestrus = Day 0). Plasma progesterone (P4) concentrations were measured daily. Uterine luminal fluid score was given based on the accumulation of fluid throughout the entire organ (0, no fluid, to 4). Measurement of the main ovarian structures, endometrial thickness, uterine luminal fluid score, and luminal fluid area of the cranial portion of the uterine horn ipsilateral to the preovulatory follicle and subsequent CL were executed by B-mode ultrasonography and endometrial vascularity by colour Doppler. The fixed effect of day was analysed by ANOVA. There was an effect of day on endometrial vascularity, endometrial thickness, and uterine luminal fluid score (P ≤ 0.01) as well as uterine luminal fluid area (P ≤ 0.05). Endometrial vascularity and endometrial thickness increased after PGF injection to peak on Day −1, whereas uterine fluid score peaked on Day 0. Interestingly, cranial uterine luminal fluid area peaked twice, on Day −1 and Day 5. Vascularity, thickness, and fluid accumulation decreased gradually from Day 0 to Day 5. There was a negative correlation (r = −0.55) between the sum of daily P4 concentrations during proestrus (i.e. from PGF to oestrus) and the greatest ULF score measured (P = 0.05). Length of proestrus was correlated negatively (r = −0.69) with the increase in uterine body thickness during proestrus (P = 0.03). The sum of daily luminal fluid scores, from PGF injection to Day 5, was double for heifers grouped within the upper third for that variable compared with those grouped within the lower third. In conclusion, peri-ovulatory transformations in uterine morphology and function are dramatic and vary in duration and in intensity among animals. Temporal changes in intraluminal fluid accumulation and absorption at the site of embryo arrival may change its biochemical composition. Potential implications for embryo receptivity and reproductive outcomes deserve further investigation.}, number={2}, booktitle={Reproduction, Fertility and Development}, publisher={CSIRO Publishing}, author={Silva, F. A. C. C. and Silva, G. F. and Vieira, B. S. and Neto, A. L. and Junior, G. A. Arantes and Silva, A. G. and Nogueira, G. P. and Pugliesi, G. and Binelli, M.}, year={2021}, pages={131} }
 @article{binelli_gonella-diaza_martins_rocha_silva_tarnonsky_roskopf_rae_driver_dilorenzo_et al._2021, title={Calculating Reproductive Performance in Beef Operations: The University of Florida Beef Herds' 2019 Breeding Season}, volume={2021}, url={http://dx.doi.org/10.32473/edis-an365-2021}, DOI={10.32473/edis-an365-2021}, abstractNote={This publication reviews the basic calculations of reproductive performance and reports the reproductive performance of two University of Florida beef herds. Written by Mario Binelli, Angela M. Gonella-Diaza, Thiago Martins, Cecilia C. Rocha, Felipe A. C. C. Silva, Federico Tarnonsky, Sergio Roskopf, Owen Rae, Danny Driver, Nicolas DiLorenzo, Jose Dubeux, and David Thomas, and published by the UF/IFAS Department of Animal Sciences, May 2021.}, number={3}, journal={EDIS}, publisher={University of Florida George A Smathers Libraries}, author={Binelli, Mario and Gonella-Diaza, Angela Maria and Martins, Thiago and Rocha, Cecilia C. and Silva, Felipe A.C.C. and Tarnonsky, Federico and Roskopf, Sergio and Rae, Owen D. and Driver, Danny and DiLorenzo, Nicolas and et al.}, year={2021}, month={Jun} }
 @article{binelli_gonella-diaza_martins_rocha_silva_rae_driver_dilorenzo_dubeux_thomas_2021, title={Calf Performance of the University of Florida beef herds in the 2019-2020 calving season}, volume={85}, number={8}, journal={The Florida Cattleman and Livestock Journal}, author={Binelli, M. and Gonella-Diaza, A. and Martins, T. and Rocha, C.C. and Silva, F.A.C.C. and Rae, F.O. and Driver, J.D. and DiLorenzo, N. and Dubeux, J. and Thomas, D.}, year={2021}, pages={32} }
 @article{binelli_gonella-diaza_martins_rocha_silva_rae_driver_dilorenzo_dubeux_thomas_2021, title={Florida yearling beef heifer reproductive performance benchmarks}, volume={85}, number={12}, journal={The Florida Cattleman}, author={Binelli, M. and Gonella-Diaza, A.M. and Martins, T. and Rocha, C.C. and Silva, F.A.C.C. and Rae, O.D. and Driver, D. and DiLorenzo, N. and Dubeux, J. and Thomas, D.}, year={2021}, pages={59–60} }
 @article{mayo_alves_gonella_mauldin_2021, title={Genetic Testing of Beef Cattle for Parentage, Inherited Traits, and Enhanced EPDs}, volume={10}, number={33}, journal={Panhandle Ag News}, author={Mayo, D. and Alves, F. and Gonella, A. and Mauldin, M.}, year={2021} }
 @inproceedings{silva_martins_sponchiado_pohler_penagaricano_binelli_2021, place={In}, title={Increased concentration of progesterone prior to luteolysis affects uterine programming during the subsequent luteal phase in beef cattle}, booktitle={SSR 54th Annual Meeting Reproductive Biology:  Solutions for Adult Disease}, publisher={SSR}, author={Silva, F.A.C.C. and Martins, T. and Sponchiado, M. and Pohler, K.G. and Penagaricano, F. and Binelli, M.}, year={2021} }
 @inproceedings{silva_silva_vieira_neto_rocha_junior_silva_turco_nogueira_pugliesi_et al._2021, title={PSV-4 Peri-estrus ovarian, uterine, and hormonal variables influence the uterine luminal fluid metabolome in beef heifers}, volume={99}, url={http://dx.doi.org/10.1093/jas/skab235.568}, DOI={10.1093/jas/skab235.568}, abstractNote={
 In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Overarching hypothesis was that peri-estrus changes in ULF volume through accumulation and resorption mechanisms influence ULF composition during the estrous cycle and early pregnancy. Objectives were (1) to characterize individual temporal and spatial changes in ULF volume, endometrial and luteal vascularity, endometrial and luteal size, and progesterone (P4) concentrations during the peri-estrus period in beef heifers and, (2) associate such changes with the metabolite composition in the ULF, four days after estrus. Fourteen Bos indicus heifers that presented a PGF2α responsive CL received 500 µg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (estrus = d 0). Plasma P4 was measured daily. On d 4, the uterine body was sampled using a cytology brush for targeted metabolomic analysis by mass spectrometry. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Individual metabolite concentrations were compared between clusters A and B by univariate analysis using t-test after FDR correction. Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of non-essential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and associated uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers, which was associated with the ULF composition four days after estrus. Potential implications for embryo receptivity and reproductive outcomes are the focus of the current investigation.}, number={Supplement_3}, booktitle={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Silva, Felipe Alves Correa Carvalho and Silva, Gabriela and Vieira, Bruna and Neto, Adomar and Rocha, Cecilia Constantino and Junior, Gilmar Arantes and Silva, Amanda and Turco, Edson Lo and Nogueira, Guilherme D and Pugliesi, Guilherme and et al.}, year={2021}, month={Oct}, pages={309–310} }
 @inproceedings{rocha_silva_martins_marrero_driver_bromfield_hansen_binelli_2021, title={PSXVI-12 Revolutionizing in vitro endometrial cell studies: A novel methodology to obtain bovine endometrial cells}, volume={99}, DOI={10.1093/jas/skab235.598}, abstractNote={
 Cultured primary endometrial cells are used extensively to study uterine function in cattle. However, most protocols harvest endometrial cells from slaughtered animals at estimated stages of the estrous cycle. The goal of this study was to establish and validate an in vivo, minimally invasive, and estrous cycle stage-specific method to obtain endometrial cells for culture. In Experiment 1, the uterine body of Bos indicus-influenced cows was sampled using a cytology brush (cytobrush) 4 days post estrus (D4; n = 13). Brushes were transported in medium (DMEM/F12, 3% Penicillin/Streptomycin and 2% of Fungizone) to the laboratory at ambient temperature. Cells were cultured in medium containing 10% FBS at 5% of CO2 (38°C). Confluent cells (~7 days of culture) were sub-cultured for two subsequent passages. Pools (n = 4) of cells from 2–3 animals, were frozen, thawed, and re-plated (passage 3). The relative transcript abundance of PPIA, ACTB, KRT18, VIM, OXTR, PGR, ESR1 and IFNAR1 were analyzed by qPCR and compared among fresh cells and cells from each passage. Abundance of KRT18 and VIM transcripts was similar across passages, while PGR, ESR1, OXTR and IFNAR1 transcripts decreased by 90, 96, 84, and 82 %; respectively in cultured compared to fresh cells (P < 0.05). In Experiment 2, passage 3 cells were cultured for 24 hours with 0 or 1ng/mL of recombinant bovine interferon-tau (rbIFNT; n = 3 replicates/treatment). The relative expression of a classical interferon stimulated gene, ISG15, was evaluated by qPCR. Expression of ISG15 was 6-fold greater (P < 0.05) in the rbIFNT treated cells compared to controls. In conclusion, the culture of endometrial cells collected by cytobrush is feasible, generates a monolayer enriched in epithelial cells and may be used as a model for physiological studies involving IFNT signaling. Further experiments to ascertain the physiological relevance of this model are underway.}, number={S3}, booktitle={Journal of Animal Science}, author={Rocha, C.C. and Silva, F.A.C.C. and Martins, T. and Marrero, M. and Driver, J. and Bromfield, J. and Hansen, T.R. and Binelli, M.}, year={2021}, month={Nov}, pages={325–326} }
 @article{silva_silva_vieira_neto_rocha_turco_nogueira_pugliesi_binelli_2021, title={Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers}, volume={8}, url={http://dx.doi.org/10.1093/biolre/ioab149}, DOI={10.1093/biolre/ioab149}, abstractNote={Abstract In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 µg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus. Summary sentence Uterine luminal fluid metabolome 4 days after estrus presented contrasting composition in heifers clustered by differences in peri-estrus ovarian, uterine, and hormonal variables. Graphical Abstract}, journal={Biology of Reproduction}, publisher={Oxford University Press (OUP)}, author={Silva, Felipe A C C and Silva, Gabriela F and Vieira, Bruna S and Neto, Adomar L and Rocha, Cecilia C and Turco, Edson G Lo and Nogueira, Guilherme P and Pugliesi, Guilherme and Binelli, Mario}, year={2021}, month={Aug} }
 @article{martins_silva_soriano_pinto_rocha_gonella-diaza_dilorenzo_rae_binelli_2021, title={Puberty attainment and reproductive performance of yearling Bos indicus-influenced heifers after two sequential treatments with progesterone}, volume={231}, url={http://dx.doi.org/10.1016/j.anireprosci.2021.106803}, DOI={10.1016/j.anireprosci.2021.106803}, abstractNote={Number of pubertal heifers at time of breeding season initiation is a primary determinant to pregnancy success during the breeding season. It was hypothesized that pre-breeding progesterone (P4) supplementation (induction) would increase the number of heifers pubertal at the time of imposing estrous synchronization treatment regimens and P/AI. Yearling, Bos indicus-influenced (n = 577) or Bos indicus (n = 174) heifers were or were not treated with P4 (CIDR and Non-CIDR, respectively) for 10 d starting on D-23 (D0 = TAI). Presence of a CL on D-33 or D-23 was considered to indicate heifers were pubertal. On D-13, there was a PGF analogue administered. On D-9, there was treatment with GnRH analogue, 6d-CIDR and PGF. There were inseminations based on estrus (D-2 to D0) or TAI on D0 for non-estrous animals. There were 5.2 % and 62.9 % purebred and crossbred heifers pubertal, respectively. Proportion of prepubertal crossbred than purebred heifers with CL on D-3 was greater as a result of imposing the pubertal induction regimen (P < 0.05 and P> 0.10, respectively). Regardless of puberty status, proportion of heifers in estrus prior to AI in the CIDR group was similar to the heifers of the Non-CIDR group for crossbreds and purebreds. Similarly, P/AI of CIDR group was similar to the Non-CIDR group for crossbreds and purebreds. In summary, imposing the pubertal induction regimen hastened attainment of puberty in yearling crossbred, but not purebred heifers. Puberty induction did not affect estrous response, neither fertility after imposing an estrous synchronization treatment regimen.}, journal={Animal Reproduction Science}, publisher={Elsevier BV}, author={Martins, T. and Silva, F.A.C.C. and Soriano, G.A. and Pinto, L.M.F. and Rocha, C.C. and Gonella-Diaza, A.M. and DiLorenzo, N. and Rae, O. and Binelli, M.}, year={2021}, month={Aug}, pages={106803} }
 @article{binelli_gonella-diaza_martins_rocha_silva_tarnonsky_roskopf_rae_driver_dilorenzo_et al._2021, title={Reproductive Performance of the University of Florida beef herds in the 2019 breeding season}, volume={85}, number={6}, journal={The Florida Cattleman and Livestock Journal}, author={Binelli, M. and Gonella-Diaza, A. and Martins, T. and Rocha, C.C. and Silva, F.A.C.C. and Tarnonsky, F. and Roskopf, S. and Rae, O. and Driver, J.D. and DiLorenzo, N. and et al.}, year={2021}, pages={14} }
 @article{binelli_martins_rocha_silva_bittar_moriel_gonella-diaza_butler_sanders_2021, title={Reproductive Tract Score: A Tool for Evaluating Beef Heifer Reproductive Potential}, url={https://doi.org/10.32473/edis-an370-2021}, DOI={10.32473/edis-an370-2021}, abstractNote={This publication explains the RTS methodology and suggests how it can be implemented in a cow-calf operation. Written by Mario Binelli, Thiago Martins, Cecilia C. Rocha, Felipe A. C. C. Silva, João Bittar, Philipe Moriel, Angela M. Gonella-Diaza, Lauren Butler, and Cindy Sanders, and published by the UF/IFAS Department of Animal Sciences, October 2021.}, journal={EDIS}, author={Binelli, Mario and Martins, Thiago and Rocha, Cecilia Constantino and Silva, Felipe A. C. C. and Bittar, João and Moriel, Philipe and Gonella-Diaza, Angela and Butler, Lauren and Sanders, Cindy}, year={2021}, month={Nov} }
 @article{martins_da silva_sponchiado_soriano_pinto_rocha_podversich_tarnosky_lenin_driver_et al._2020, title={PSIII-7 Determining the requirement of puberty induction to the reproductive performance of Bos indicus-influenced heifers submitted to AI}, volume={98}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skaa278.425}, DOI={10.1093/jas/skaa278.425}, abstractNote={
 Induction of puberty by treating heifers with progesterone (P4) inserts may favor pregnancy/AI (P/AI) to a subsequent estrus synchronization program. However, it remains to be elucidated whether induction is required when the synchronization program contains P4 supplementation. To address this question, yearling, Brahman-influenced heifers (mostly 3/8 Brahman) classified as pubertal (PUB; n = 363) or prepubertal (PRE; n = 214) based on the presence of a CL, were assigned to receive no P4 supplementation (NoCIDR) or a CIDR for 10 days, starting on D-23. On D-13, all heifers received a dose of prostaglandin analogue and CIDRs were removed. On D-9, all heifers were enrolled in a 6-day CIDR & TAI protocol. Heifers were serviced based on heat between D-2 and D0 or were TAI on D0. Reproductive tract scores (RTS, 1 to 5 scale) were evaluated on D-23 and D-3. Induction increased the proportion of PRE heifers bearing a CL on D-3 (PRE|CIDR: 60.3% vs. PRE|NoCIDR: 42.7%). Among heifers with CL on D-3, P/AI of PRE heifers (42.5%) was similar to that PUB (47.7%). Conversely, for heifers without CL on D-3, P/AI of PRE (14.7%) was lower than PUB (37.3%). RTS increased between D-23 and D-3 from 2.3 to 4.0 (CIDR) and 2.3 to 3.6 (NoCIDR). For every 1-point increase in RTS, P/AI was 6–9% greater. Despite hastened puberty, P/AI of PRE heifers were less (PRE|NoCIDR: 29.2% and PRE|CIDR: 30.4%) than that of PUB (PUB|NoCIDR: 50.7% and PUB|CIDR: 42.2%). Less PRE (42.5%) than PUB animals (69.7%) were AI at estrus, and heifers showing estrus had a higher P/AI (52.8% vs. 18.7%). In conclusion, induction is not required when the subsequent synchronization protocol for AI contains progesterone. Induction hastened puberty attainment but failed to improve estrus response and fertility to the synchronization protocol.}, number={Supplement_4}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Martins, Thiago and da Silva, Felipe A C C and Sponchiado, Mariana and Soriano, Gabriela A and Pinto, Leonardo M F and Rocha, Cecilia C and Podversich, Federico and Tarnosky, Federico and Lenin, Yasser and Driver, Joseph D and et al.}, year={2020}, month={Nov}, pages={231–232} }
 @article{binelli_martins_silva_soriano_pinto_rocha_gonella-diaza_dilorenzo_rae_2020, title={Puberty induction as a strategy to increase reproductive performance of Brahman-influenced heifers}, volume={85}, number={3}, journal={The Florida Cattleman and Livestock Journal}, author={Binelli, M. and Martins, T. and Silva, F.A.C.C. and Soriano, G.A. and Pinto, L.M.F. and Rocha, C.C. and Gonella-Diaza, A.M. and DiLorenzo, N. and Rae, O.}, year={2020}, pages={22} }
 @book{binelli_martins_azenha milani soriano_sponchiado_alves correa carvalho da silva_lenis sanin_2019, title={Reproductive Challenges in Cow-calf Operations}, institution={Florida Beef Cattle Short Course}, author={Binelli, M. and Martins, T. and Azenha Milani Soriano, G. and Sponchiado, M. and Alves Correa Carvalho da Silva, F. and Lenis Sanin, Y.}, year={2019} }
 @book{binelli_martins_azenha milani soriano_sponchiado_silva_lenis sanin_2019, title={Reproductive Challenges in Cow-calf Operations}, publisher={Florida Beef Cattle Short Course}, author={Binelli, M. and Martins, T. and Azenha Milani Soriano, G. and Sponchiado, M. and Silva, F. and Lenis Sanin, Y.}, year={2019} }
 @article{da silva_negrin-pereira_funnell_baumgaertner_crosswhite_mccarthy_underdahl_neville_sedivec_degrofft_et al._2018, title={131 The effects of injectable trace mineral supplements in donor cows at the initiation of a superovulation protocol in embryo outcomes and pregnancy rates in recipient females.}, volume={96}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/sky404.762}, DOI={10.1093/jas/sky404.762}, abstractNote={We evaluated the effects of administering injectable trace mineral supplements at the initiation of a superovulation protocol on embryo outcomes (Exp 1) and pregnancy rates (Exp 2). In Exp 1, 35 crossbred Angus cows were randomly assigned to one of two treatments at initiation of a 16 d superovulation protocol; 1) cows received 90 mg Cu, 60 mg Mn, 30 mg Se, and 360 mg Zn as an injectable TM supplement (6 ml Multimin 90 s.q.; ITM); or 2) were untreated (CON). All donors were exposed to a common superovulation protocol, embryos were recovered 7 d after AI, graded for quality and developmental stage, and frozen until transfer. A replicated crossover design was used, with 120 d between flushes (total n = 70 for CON and 69 for ITM). In Exp 2, crossbred beef females were synchronized to receive embryos, and females with a CL 7 d after induced ovulation randomly assigned to receive an embryo originating from; 1) donors exposed to ITM (n =196); or 2) donors exposed to CON (n = 212). Embryo recovery data were analyzed using PROC MIXED of SAS for main effects of treatment, period, and their interaction, whereas the GLIMMIX procedure of SAS was used to analyze recipient pregnancy rates. In Exp 1, number of viable embryos recovered from cows responding to superovulation was similar (P = 0.94) among ITM (4.53 ± 0.63) and CON cows (4.46 ± 0.63). Moreover, number of degenerate, unfertilized, and total ova collected (P ≥ 0.67), and number of embryos in respective developmental stages or quality grades (P ≥ 0.24) were not affected by treatment. In Exp 2, pregnancy rates to embryo transfer were similar (P = 0.52) among CON (49.1%) and ITM (45.9 %) treatments. In this study, ITM administration failed to enhance embryo outcomes or pregnancy rates.}, number={suppl_3}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={da Silva, F and Negrin-Pereira, N and Funnell, B and Baumgaertner, F and Crosswhite, M and McCarthy, K and Underdahl, S and Neville, B and Sedivec, K and DeGrofft, D and et al.}, year={2018}, month={Dec}, pages={346–346} }
 @book{da silva_negrin-pereira_crosswhite_mccarthy_underdahl_baumgaertner_neville_degrofft_dahlen_2018, title={The effects of injectable trace mineral supplements in donor cows at the initiation of a superovulation protocol in embryo outcomes and pregnancy rates in recipient females}, institution={NDSU Central Grasslands Research Extension Center Annual Report}, author={da Silva, F.A.C.C. and Negrin-Pereira, N. and Crosswhite, M.R. and McCarthy, K.L. and Underdahl, S.R. and Baumgaertner, F. and Neville, B.W. and DeGrofft, D. and Dahlen, C.R.}, year={2018} }
 @book{silva_negrin-pereira_crosswhite_mccarthy_underdahl_baumgaertner_neville_degrofft_dahlen_2018, title={The effects of injectable trace mineral supplements in donor cows at the initiation of a superovulation protocol in embryo outcomes and pregnancy rates in recipient females}, publisher={NDSU Central Grasslands Research Extension Center Annual Report}, author={Silva, F.A.C.C. and Negrin-Pereira, N. and Crosswhite, M.R. and McCarthy, K.L. and Underdahl, S.R. and Baumgaertner, F. and Neville, B.W. and DeGrofft, D. and Dahlen, C.R.}, year={2018} }
 @inproceedings{alves correa_camacho_lemley_hallford_swanson_vonnahme_2017, title={Restriction and realimentation in pregnant beef cows: Impacts on mammary gland hemodynamics}, volume={68}, booktitle={Proceedings of the American Society of Animal Science  Western Section}, author={Alves Correa, F. and Camacho, L.E. and Lemley, C.O. and Hallford, D.M. and Swanson, K.C. and Vonnahme, K.A.}, year={2017}, pages={219–221} }
 @inproceedings{romanzini_valente_rabelo_barbero_souza_silva_santos_reis_2013, title={Efeito de diferentes fontes lipídicas no comportamento ingestivo de bovinos confinados}, booktitle={XXIII Congresso Brasileiro de Zootecnia}, author={Romanzini, E.P. and Valente, A. and Rabelo, C. and Barbero, R. and Souza, F. and Silva, F. and Santos, S. and Reis, R.}, year={2013} }
 @inproceedings{silva_oliveira_borges_santos_almeida_silva_2011, title={Manejo de pequenos rebanhos de gado leiteiro: estudo de caso e propostas para melhoria}, booktitle={V Congresso Latino Americano e XI Congresso Brasileiro de Higienista de Alimentos}, author={Silva, F.A.C.C. and Oliveira, G.C. and Borges, J.B. and Santos, M.Q.P. and Almeida, H.M.S. and Silva, D.V.A.}, year={2011} }
 @inproceedings{silva_oliveira_borges_santos_almeida_silva_2011, title={Manejo de pequenos rebanhos de gado leiteiro: estudo de caso e propostas para melhoria}, booktitle={V Congresso Latino Americano e XI Congresso Brasileiro de Higienista de Alimentos}, author={Silva, F.A.C.C. and Oliveira, G.C. and Borges, J.B. and Santos, M.Q.P. and Almeida, H.M.S. and Silva, D.V.A.}, year={2011} }