Fu-Chyun Chu

Works (8)

Updated: April 5th, 2024 10:45

2023 journal article

An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, Diabrotica virgifera virgifera

Insects, 14(8), 683.

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n, A. Cohen n  & M. Lorenzen n 

author keywords: insect rearing; rootworm; molecular biology; function genomics; CRISPR
TL;DR: An optimized rearing system for use in western corn rootworm functional genomics research is reported, specifically the development of a system that facilitates the collection of preblastoderm embryos for microinjection as well as gathering large larvae and pupae for downstream phenotypic screening. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: September 11, 2023

2023 journal article

Genome and Genetic Engineering of the House Cricket (Acheta domesticus): A Resource for Sustainable Agriculture

Biomolecules, 13(4), 589.

By: A. Dossey, B. Oppert *, F. Chu*, M. Lorenzen n , B. Scheffler *, S. Simpson *, S. Koren *, J. Johnston *, K. Kataoka *, K. Ide *

author keywords: food security; cricket; protein; genome; sustainability; agriculture; insects as food and feed; Acheta domesticus; insect genome; CRISPR-Cas9; genetic engineering
MeSH headings : Animals; Gryllidae / genetics; Gryllidae / metabolism; Agriculture; Crops, Agricultural; Allergens / metabolism; Genetic Engineering
TL;DR: The first high quality annotated genome assembly of A. domesticus is presented, demonstrating both CRISPR/Cas9-mediated knock-in and knock-out of the house cricket and implications for the food, pharmaceutical, and other industries. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science; OpenAlex)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: March 29, 2023

2023 journal article

Structural and functional insights into the ATP-binding cassette transporter family in the corn planthopper, Peregrinus maidis

Insect Molecular Biology, 32(4), 412–423.

By: Y. Wang n, W. Klobasa n, F. Chu n, O. Huot n, A. Whitfield n & M. Lorenzen n 

author keywords: ABC transporters; Hemiptera; RNA interference (RNAi); transcriptome
TL;DR: Transcriptomic data and RNAi results presented here will no doubt assist with the development of new control methods for this pest and each phenotype is similar to that found when targeting the orthologous gene in other species and it demonstrates their potential in RNAi‐based P. maidis control. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 1, 2023

2023 journal article

The Genome of the Yellow Mealworm, <i>Tenebrio molitor</i>: It's Bigger Than You Think

GENES, 14(12).

By: B. Oppert *, A. Dossey *, F. Chu*, E. Satovic-Vuksic, M. Plohl *, T. Smith *, S. Koren *, M. Olmstead*  ...

author keywords: CRISPR Cas9 gene editing; insects as food and feed; insect genomics and genetics; stored product insect; Tenebrio molitor; yellow mealworm
TL;DR: The genome of T. molitor is longer than current assemblies due to a substantial amount of only one highly abundant satellite DNA sequence, which will promote the sustainable utilization of mealworms and other farmed insects. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 16, 2024

2021 journal article

Microinjection of Corn Planthopper, <em>Peregrinus maidis</em>, Embryos for CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, 3(169).

By: W. Klobasa n, F. Chu n, O. Huot n, N. Grubbs n, D. Rotenberg n , A. Whitfield n, M. Lorenzen n 

MeSH headings : Animals; CRISPR-Cas Systems / genetics; Endonucleases / genetics; Female; Gene Editing / methods; Zea mays / chemistry
TL;DR: Detailed protocols for collecting and microinjecting precellular P. Maidis embryos with CRISPR components (Cas9 nuclease that has been complexed with guide RNAs), and results of Cas9-based gene knockout of a P. maidis eye-color gene, white, are presented. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 17, 2021

2019 journal article

Effects of targeting eye color in Tenebrio molitor through RNA interference of tryptophan 2,3‐dioxygenase ( vermilion ): Implications for insect farming

Archives of Insect Biochemistry and Physiology, 101(1), e21546.

By: B. Oppert *, F. Chu n, S. Reyna n, S. Pinzi n, S. Adrianos*, L. Perkin *, M. Lorenzen n 

author keywords: agriculture; entomophagy; farming; gene expression; insect eye color; protein; RNA-Seq; Tenebrio molitor; vermilion
MeSH headings : Animal Husbandry; Animals; Eye Color / genetics; Gene Expression; Genetic Markers; Insect Proteins / genetics; Insect Proteins / metabolism; Larva / genetics; Pigmentation / genetics; Pupa / genetics; RNA Interference; Tenebrio / enzymology; Tenebrio / genetics; Tenebrio / growth & development; Tryptophan Oxygenase / genetics
TL;DR: A dominant marker system based on eye color can be developed for the genetic manipulation of T. molitor to increase the value of mealworms as an alternative food source by decreasing negative traits,such as disease susceptibility, and increasing desired traits, such as protein content and vitamin production. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 15, 2019

2018 journal article

Microinjection of Western Corn Rootworm, <em>Diabrotica virgifera virgifera</em>, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, (134).

By: F. Chu n, P. Wu n, S. Pinzi n, N. Grubbs n & M. Lorenzen n 

author keywords: Bioengineering; Issue 134; embryonic microinjection; functional genomics; germline transformation; transgenesis; CRISPR/Cas9 genome editing; western corn rootworm
MeSH headings : Animals; CRISPR-Cas Systems / genetics; Coleoptera; Gene Editing / methods; Germ-Line Mutation / genetics; Microinjections / methods; RNA Interference
TL;DR: The methods used to collect and microinject precellular WCR embryos with nucleic acids are detailed, aimed at the creation of transgenic WCR, and CRISPR/Cas9-genome editing could also be performed using the same protocols, with the only difference being the composition of the solution injected into the embryos. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: October 16, 2018

2017 journal article

Development and use of a piggyBac -based jumpstarter system in Drosophila suzukii

Archives of Insect Biochemistry and Physiology, 97(3), e21439.

By: F. Chu n, W. Klobasa n, N. Grubbs n & M. Lorenzen n 

author keywords: germline transformation; Minos; mutagenesis; piggyBac; transgenesis
MeSH headings : Animals; Animals, Genetically Modified; DNA Transposable Elements; Drosophila / genetics; Genetic Engineering / methods; Mutagenesis; Transposases
TL;DR: It is demonstrated that there is a dramatic increase in transformation rates when germline transformation is performed in a transposase-expressing background, and it is shown that these jumpstarter strains could be used in conjunction with a piggyBac-based donor strain for genome-wide mutagenesis of D. suzukii. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

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