Flora Meilleur

https://orcid.org/0000-0001-9313-8989

protein crystallography, neutron diffraction, enzymatic mechanism, metallo-enzyme, LPMO, P450

Works (57)

2019 journal article

A prototype system for dynamically polarized neutron protein crystallography

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 940, 430–434.

By: J. Pierce, L. Crow, M. Cuneo, M. Edwards, K. Herwig, A. Jennings, A. Jones, L. Li ...

Sources: Web Of Science, ORCID
Added: July 29, 2019

2019 journal article

Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 75(3), 171–175.

By: X. Lu, B. Selvaraj, S. Ghimire-Rijal, G. Orf, F. Meilleur, R. Blankenship, M. Cuneo, D. Myles

Sources: Web Of Science, ORCID
Added: March 25, 2019

2019 journal article

Titration of ionizable groups in proteins using multiple neutron data sets from a single crystal: application to the small GTPase Ras

Acta Crystallographica Section F Structural Biology Communications, 75(2), 111–115.

By: R. Knihtila, A. Volmar, F. Meilleur & C. Mattos

Sources: NC State University Libraries, ORCID
Added: February 18, 2019

2018 journal article

A suite-level review of the neutron single-crystal diffraction instruments at Oak Ridge National Laboratory

Review of Scientific Instruments, 89(9).

By: L. Coates, H. Cao, B. Chakoumakos, M. Frontzek, C. Hoffmann, A. Kovalevsky, Y. Liu, F. Meilleur ...

Sources: NC State University Libraries, ORCID
Added: October 16, 2018

2018 journal article

Modulating Enzyme Activity by Altering Protein Dynamics with Solvent

Biochemistry, 57(29), 4263–4275.

By: M. Duff, J. Borreguero, M. Cuneo, A. Ramanathan, J. He, G. Kamath, S. Chennubhotla, F. Meilleur ...

Sources: Crossref, ORCID
Added: February 18, 2020

2018 journal article

Neutron scattering in the biological sciences: progress and prospects

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 74, 1129–1168.

By: R. Ashkar, H. Bilheux, H. Bordallo, R. Briber, D. Callaway, X. Cheng, X. Chu, J. Curtis ...

Sources: NC State University Libraries, ORCID
Added: January 21, 2019

2018 journal article

Next-generation diamond cell and applications to single-crystal neutron diffraction

REVIEW OF SCIENTIFIC INSTRUMENTS, 89(9).

By: B. Haberl, S. Dissanayake, Y. Wu, D. Myles, A. Santos, M. Loguillo, G. Rucker, D. Armitage ...

Sources: NC State University Libraries, ORCID
Added: October 16, 2018

2018 journal article

Structural investigation of cellobiose dehydrogenase IIA: Insights from small angle scattering into intra- and intermolecular electron transfer mechanisms

Biochimica Et Biophysica Acta. G, General Subjects, 1862(4), 1031–1039.

By: A. Bodenheimer, W. Odell, R. Oliver, S. Qian, C. Stanley & F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2018 journal article

The Neutron Macromolecular Crystallography Instruments at Oak Ridge National Laboratory: Advances, Challenges, and Opportunities

CRYSTALS, 8(10).

By: F. Meilleur, L. Coates, M. Cuneo, A. Kovalevsky & D. Myles

Sources: NC State University Libraries, ORCID
Added: November 19, 2018

2017 journal article

An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase

Scientific Reports, 7(1), 40517,

By: E. Golden, L. Yu, F. Meilleur, M. Blakeley, A. Duff, A. Karton, A. Vrielink

Sources: ORCID, Crossref
Added: January 6, 2020

2017 journal article

Crystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction

Acta Crystallographica Section F Structural Biology Communications, 73(2), 70–78.

By: W. O'Dell, P. Swartz, K. Weiss & F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Innentitelbild: Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase (Angew. Chem. 3/2017)

Angewandte Chemie, 129(3), 674–674.

By: W. O'Dell, P. Agarwal & F. Meilleur

Source: ORCID
Added: January 6, 2020

2017 journal article

Inside Cover: Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase (Angew. Chem. Int. Ed. 3/2017)

Angewandte Chemie International Edition, 56(3), 658–658.

By: W. O'Dell, P. Agarwal & F. Meilleur

Source: ORCID
Added: January 6, 2020

2017 journal article

Neutron crystallographic studies of T4 lysozyme at cryogenic temperature

Protein Science, 26(10), 2098–2104.

By: L. Li, S. Shukla, F. Meilleur, R. Standaert, J. Pierce, D. Myles, M. Cuneo

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Neutron structure of the T26H mutant of T4 phage lysozyme provides insight into the catalytic activity of the mutant enzyme and how it differs from that of wild type

Protein Science, 26(10), 1953–1963.

By: T. Hiromoto, F. Meilleur, R. Shimizu, C. Shibazaki, M. Adachi, T. Tamada, R. Kuroki

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase

Angewandte Chemie International Edition, 56(3), 767–770.

By: W. O'Dell, P. Agarwal & F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase

Angewandte Chemie, 129(3), 785–788.

By: W. O'Dell, P. Agarwal & F. Meilleur

Source: ORCID
Added: January 6, 2020

2017 journal article

Structural studies of Neurospora crassa LPMO9D and redox partner CDHIIA using neutron crystallography and small-angle scattering

Carbohydrate Research, 3, 200–204.

By: A. Bodenheimer, W. O'Dell, C. Stanley & F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2016 journal article

Crystal structures of wild-type Trichoderma reesei Cel7A catalytic domain in open and closed states

FEBS Letters, 590(23), 4429–4438.

By: A. Bodenheimer & F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2016 journal article

Detection of mycoplasma haemocanis, mycoplasma haematoparvum, mycoplasma suis and other vector-borne pathogens in dogs from cordoba and santa fe, argentina

Parasites & Vectors, 9.

By: P. Mascarelli, G. Tartara, N. Pereyra & R. Maggi

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2016 journal article

Neutron protein crystallography: A complementary tool for locating hydrogens in proteins

Archives of Biochemistry and Biophysics, 602, 48–60.

By: W. O'Dell, A. Bodenheimer & F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Neutron Crystal Structure of RAS GTPase Puts in Question the Protonation State of the GTP γ-Phosphate

Journal of Biological Chemistry, 290(52), 31025–31036.

By: R. Knihtila, G. Holzapfel, K. Weiss, F. Meilleur & C. Mattos

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Production and characterization of recombinant perdeuterated cholesterol oxidase

Analytical Biochemistry, 485, 102–108.

By: E. Golden, P. Attwood, A. Duff, F. Meilleur & A. Vrielink

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

Crystallization and preliminary X-ray diffraction analysis of Hypocrea jecorina Cel7A in two new crystal forms.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 70(Pt 6), 773–776.

By: A. Bodenheimer, M. Cuneo, P. Swartz, J. He, H. O'Neill, D. Myles, B. Evans, F. Meilleur, F. Section

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

Neutron structure of the cyclic glucose-bound xylose isomerase E186Q mutant.

Acta Crystallographica. Section D, Biological Crystallography, 70(Pt 2), 414–420.

By: P. Munshi, E. Snell, M. Woerd, R. Judge, D. Myles, Z. Ren, F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2014 journal article

ORNL hosts the participants of the 4thNeutrons in Structural Biology Workshop and the IMAGINE single crystal neutron diffractometer first external users

Neutron News, 25(1), 12–12.

By: F. Meilleur

Source: ORCID
Added: January 6, 2020

2014 journal article

The Neutrons in Structural Biology Workshop celebrates its 5th edition

Neutron News, 25(4), 11–11.

By: F. Meilleur

Source: ORCID
Added: January 6, 2020

2013 article

Neutron Scattering Techniques and Applications in Structural Biology

Ankner, J. F., Heller, W. T., Herwig, K. W., Meilleur, F., & Myles, D. A. A. (2013, April). Current Protocols in Protein Science.

By: J. Ankner, W. Heller, K. Herwig, F. Meilleur & D. Myles

Source: ORCID
Added: January 6, 2020

2013 journal article

New insight into the structure of RNA in red clover necrotic mosaic virus and the role of divalent cations revealed by small-angle neutron scattering.

Archives of Virology, 158(8), 1661–1669.

By: S. Martin, L. He, F. Meilleur, R. Guenther, T. Sit, S. Lommel, W. Heller

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Refinement of macromolecular structures against neutron data withSHELXL2013

Journal of Applied Crystallography, 47(1), 462–466.

By: T. Gruene, H. Hahn, A. Luebben, F. Meilleur & G. Sheldrick

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

The IMAGINE instrument: first neutron protein structure and new capabilities for neutron macromolecular crystallography.

Section D, Biological Crystallography, 69(Pt 10), 2157–2160,

By: F. Meilleur, P. Munshi, L. Robertson, A. Stoica, L. Crow, A. Kovalevsky, T. Koritsanszky, B. Chakoumakos, R. Blessing, D. Myles

Source: ORCID
Added: January 6, 2020

2013 journal article

Third School on the Applications of Neutron Scattering Techniques in Structural Biology, Oak Ridge, TN

Neutron News, 24(1), 4–4.

By: F. Meilleur

Source: ORCID
Added: January 6, 2020

2012 journal article

Conformational changes in Sindbis virus induced by decreased pH are revealed by small-angle neutron scattering.

Journal of Virology, 86(4), 1982–1987.

By: L. He, A. Piper, F. Meilleur, R. Hernandez, W. Heller & D. Brown

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Neutron protein crystallography at ultra-low (<15 K) temperatures

Journal of Applied Crystallography, 45(4), 686–692.

By: D. Myles, F. Dauvergne, M. Blakeley & F. Meilleur

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering.

Journal of Biological Chemistry, 287(44), 37119–37133.

By: J. Jayasundar, J. Ju, L. He, D. Liu, F. Meilleur, J. Zhao, D. Callaway, Z. Bu

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Rapid visualization of hydrogen positions in protein neutron crystallographic structures.

Acta Crystallographica. Section D, Biological Crystallography, 68(Pt 1), 35–41.

By: P. Munshi, S. Chung, M. Blakeley, K. Weiss, D. Myles, F. Meilleur, D. Section

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Molecular and serological diagnosis of Bartonella infection in 61 dogs from the United States

Journal of Veterinary Internal Medicine, 25(4), 805–810.

By: C. Perez, R. Maggi, P. Diniz & E. Breitschwerdt

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Redox-promoting protein motions in rubredoxin.

Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, 115(28), 8925–8936.

By: J. Borreguero, J. He, F. Meilleur, K. Weiss, C. Brown, D. Myles, K. Herwig, P. Agarwal

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2011 journal article

Scattering functions of Platonic solids

Journal of Applied Crystallography, 44, 545–557.

By: X. Li, C. Shew, L. He, F. Meilleur, D. Myles, E. Liu, Y. Zhang, G. Smith ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2010 journal article

Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 66(Pt 11), 1458–1462.

By: S. Martin, R. Guenther, T. Sit, P. Swartz, F. Meilleur, S. Lommel, R. Rose, F. Section

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2010 journal article

The structure of Sindbis virus produced from vertebrate and invertebrate hosts as determined by small-angle neutron scattering.

Journal of Virology, 84(10), 5270–5276.

By: L. He, A. Piper, F. Meilleur, D. Myles, R. Hernandez, D. Brown, W. Heller

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2010 journal article

Unambiguous determination of H-atom positions: comparing results from neutron and high-resolution X-ray crystallography.

Acta Crystallographica. Section D, Biological Crystallography, 66(Pt 5), 558–567.

By: A. Gardberg, D. Castillo, W. Ar, M. Kl, F. Blakeley, M. Mp, A. Da

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2009 article

Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis

Micro and Nano Technologies in Bioanalysis, pp. 281–292.

By: F. Meilleur, K. Weiss & D. Myles

Source: ORCID
Added: January 6, 2020

2008 article

New sources and instrumentation for neutrons in biology (vol 345, pg 133, 2008)

Chemical Physics, Vol. 351, pp. 170–170.

By: S. Teixeira, G. Zaccai, J. Ankner, M. Bellissent-Funel, R. Bewley, M. Blakeley, P. Callow, L. Coates ...

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

New sources and instrumentation for neutrons in biology.

Chemical Physics, 345(2-3), 133–151.

By: S. Teixeira, J. Ankner, M. Bellissent-Funel, R. Bewley, M. Blakeley, L. Coates, R. Dahint, R. Dalgliesh ...

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2008 journal article

Preliminary neutron crystallographic analysis of selectively CH3-protonated deuterated rubredoxin from Pyrococcus furiosus.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 64(Pt 6), 537–540.

By: K. Weiss, F. Meilleur, M. Blakeley, D. Myles & F. Section

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2008 journal article

Quantum model of catalysis based on a mobile proton revealed by subatomic x-ray and neutron diffraction studies of h-aldose reductase.

Proceedings of the National Academy of Sciences of the United States of America, 105(6), 1844–1848,

By: M. Blakeley, F. Ruiz, R. Cachau, I. Hazemann, F. Meilleur, A. Mitschler, S. Ginell, P. Afonine ...

Source: ORCID
Added: January 6, 2020

2007 journal article

Expression, purification, assay, and crystal structure of perdeuterated human arginase I

Archives of Biochemistry and Biophysics, 465(1), 82–89.

By: L. Costanzo, M. Moulin, M. Haertlein, F. Meilleur & D. Christianson

Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2006 journal article

A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthin.

Structural Biology and Crystallization Communications, 62(Pt 3), 306–309,

By: M. Budayova-Spano, F. Bonneté, N. Ferté, M. El Hajji, F. Meilleur, M. Blakeley, B. Castro, F. Section

Source: ORCID
Added: January 6, 2020

2006 journal article

A quasi-Laue neutron crystallographic study of d-xylose isomerase

European Biophysics Journal, 35(7), 601–609.

By: F. Meilleur, E. Snell, M. Woerd, R. Judge & D. Myles

Source: ORCID
Added: January 6, 2020

2006 journal article

Comparison of hydrogen determination with X-ray and neutron crystallography in a human aldose reductase-inhibitor complex.

European Biophysics Journal : EBJ, 35(7), 577–583,

By: M. Blakeley, A. Mitschler, I. Hazemann, F. Meilleur, D. Myles & A. Podjarny

Source: ORCID
Added: January 6, 2020

2006 journal article

Neutron Laue macromolecular crystallography

European Biophysics Journal, 35(7), 611–620.

By: F. Meilleur, D. Myles & M. Blakeley

Source: ORCID
Added: January 6, 2020

2005 journal article

High-resolution neutron protein crystallography with radically small crystal volumes: application of perdeuteration to human aldose reductase.

Biological Crystallography, 61(Pt 10), 1413–1417,

By: I. Hazemann, M. Dauvergne, M. Blakeley, F. Meilleur, M. Haertlein, A. Van Dorsselaer, A. Mitschler, D. Myles, A. Podjarny, D. Section

Source: ORCID
Added: January 6, 2020

2005 journal article

Preliminary neutron diffraction studies of Escherichia coli dihydrofolate reductase bound to the anticancer drug methotrexate.

61(Pt 5), 574–579,

By: B. Bennett, F. Meilleur, D. Myles, E. Howell, C. Dealwis, D. Section, B. crystallography

Source: ORCID
Added: January 6, 2020

2005 journal article

Production and X-ray crystallographic analysis of fully deuterated cytochrome P450cam.

61(Pt 5), 539–544,

By: F. Meilleur, M. Dauvergne, I. Schlichting, D. Myles, D. Section & B. crystallography

Source: ORCID
Added: January 6, 2020

2004 journal article

Structural Stability and Dynamics of Hydrogenated and Perdeuterated Cytochrome P450cam (CYP101)†

Biochemistry, 43(27), 8744–8753.

By: F. Meilleur, J. Contzen, D. Myles & C. Jung

Source: ORCID
Added: January 6, 2020

2001 journal article

Interplay of intrinsic and environmental effects on the magnetic properties of free radicals issuing from H-atom addition to cytosine.

Journal of the American Chemical Society, 123(29), 7113–7117,

By: C. Adamo, M. Heitzmann, F. Meilleur, N. Rega, G. Scalmani, A. Grand, J. Cadet, V. Barone

Source: ORCID
Added: January 6, 2020

Employment

2015 - present

North Carolina State University Raleigh, NC, US
Associate Professor Molecular and Structural Biochemistry

2007 - present

Oak Ridge National Laboratory Oak Ridge, TN, US
Instrument Scientist - Macromolecular Crystallography Neutron Sciences Directorate

2007 - 2015

North Carolina State University Raleigh, NC, US
Assistant Professor Molecular and Structural Biochemistry

2004 - 2006

Institut Laue-Langevin Grenoble, Rhône-Alpes, FR

Education

2000 - 2004

European Molecular Biology Laboratory Grenoble, FR
Ph. D.

2000 - 2004

Université Grenoble Alpes Saint-Martin-d'Heres, Rhône-Alpes, FR
Ph. D.

Funding History

Funding history based on the linked ORCID record.

grant September 1, 2011 - August 31, 2017
IGERT: Neutron Scattering for the Science and Engineering of the 21st Century
National Science Foundation - Directorate for Education and Human Resources
grant August 1, 2009 - July 31, 2013
MRI: Acquisition of a Neutron Single Crystal Diffractometer
National Science Foundation - Directorate for Mathematical & Physical Sciences