@article{boston_wang_xi_kim_fellner_scott_ziegler_van landeghem_blikslager_odle_2023, title={Effects of Prebiotic Galactooligosaccharide (GOS) on Hindgut Microbial Composition of Pigs During the Peri-Weaning Period}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad281.228}, abstractNote={Abstract}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Boston, Timothy E. and Wang, Feng and Xi, Lin and Kim, Sung Woo and Fellner, Vivek and Scott, Mark F. and Ziegler, Amanda L. and Van Landeghem, Laurianne and Blikslager, Anthony T. and Odle, Jack}, year={2023}, month={Nov}, pages={187–188} }
 @article{boston_wang_xi_kim_fellner_scott_ziegler_van landeghem_blikslager_odle_2023, title={Effects of Prebiotic Galactooligosaccharide (Gos) on Piglet Growth and Jejunal Morphology During the Peri-Weaning Period}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad341.313}, abstractNote={Abstract}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Boston, Timothy E. and Wang, Feng and Xi, Lin and Kim, Sung Woo and Fellner, Vivek and Scott, Mark F. and Ziegler, Amanda L. and Van Landeghem, Laurianne and Blikslager, Anthony T. and Odle, Jack}, year={2023}, month={Oct}, pages={276–277} }
 @article{xi_wang_cagle_zhu_odle_xie_2023, title={Exploring the Prebiotic Activities of Proanthocyanidins on a Platform Using the Three-Dimensionally (3D)-Cultured Organoids}, volume={101}, ISSN={["1525-3163"]}, DOI={10.1093/jas/skad281.421}, abstractNote={Abstract}, journal={JOURNAL OF ANIMAL SCIENCE}, author={Xi, Lin and Wang, Feng and Cagle, Daisy and Zhu, Yue and Odle, Jack and Xie, Deyu}, year={2023}, month={Nov}, pages={355–356} }
 @article{pike_zhao_hicks_wang_hagen_liu_odle_lin_2023, title={Intestinal Carnitine Status and Fatty Acid Oxidation in Response to Clofibrate and Medium-Chain Triglyceride Supplementation in Newborn Pigs}, volume={24}, ISSN={["1422-0067"]}, url={https://doi.org/10.3390/ijms24076066}, DOI={10.3390/ijms24076066}, abstractNote={To investigate the role of peroxisome proliferator-activated receptor alpha (PPARα) in carnitine status and intestinal fatty acid oxidation in neonates, a total of 72 suckled newborn piglets were assigned into 8 dietary treatments following a 2 (±0.35% clofibrate) × 4 (diets with: succinate+glycerol (Succ), tri-valerate (TC5), tri-hexanoate (TC6), or tri-2-methylpentanoate (TMPA)) factorial design. All pigs received experimental milk diets with isocaloric energy for 5 days. Carnitine statuses were evaluated, and fatty acid oxidation was measured in vitro using [1-14C]-palmitic acid (1 mM) as a substrate in absence or presence of L659699 (1.6 µM), iodoacetamide (50 µM), and carnitine (1 mM). Clofibrate increased concentrations of free (41%) and/or acyl-carnitine (44% and 15%) in liver and plasma but had no effects in the intestine. The effects on carnitine status were associated with the expression of genes involved in carnitine biosynthesis, absorption, and transportation. TC5 and TMPA stimulated the increased fatty acid oxidation rate induced by clofibrate, while TC6 had no effect on the increased fatty acid oxidation induced by clofibrate (p > 0.05). These results suggest that dietary clofibrate improved carnitine status and increased fatty acid oxidation. Propionyl-CoA, generated from TC5 and TMPA, could stimulate the increased fatty acid oxidation rate induced by clofibrate as anaplerotic carbon sources.}, number={7}, journal={INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, author={Pike, Brandon and Zhao, Jinan and Hicks, Julie A. and Wang, Feng and Hagen, Rachel and Liu, Hsiao-Ching and Odle, Jack and Lin, Xi}, year={2023}, month={Apr} }
 @article{wang_man_wang_odle_maltecca_lin_2023, title={MicroRNA and mRNA sequencing analyses reveal key hepatic metabolic and signaling pathways responsive to maternal undernutrition in full-term fetal pigs}, volume={116}, ISSN={["1873-4847"]}, url={https://doi.org/10.1016/j.jnutbio.2023.109312}, DOI={10.1016/j.jnutbio.2023.109312}, abstractNote={Maternal undernutrition is highly prevalent in developing countries, leading to severe fetus/infant mortality, intrauterine growth restriction, stunting, and severe wasting. However, the potential impairments of maternal undernutrition to metabolic pathways in offspring are not defined completely. In this study, 2 groups of pregnant domestic pigs received nutritionally balanced gestation diets with or without 50% feed intake restriction from 0 to 35 gestation days and 70% from 35 to 114 gestation days. Full-term fetuses were collected via C-section on day 113/114 of gestation. MicroRNA and mRNA deep sequencing were analyzed using the Illumina GAIIx system on fetal liver samples. The mRNA-miRNA correlation and associated signaling pathways were analyzed via CLC Genomics Workbench and Ingenuity Pathway Analysis Software. A total of 1189 and 34 differentially expressed mRNA and miRNAs were identified between full-nutrition (F) and restricted-nutrition (R) groups. The correlation analyses showed that metabolic and signaling pathways such as oxidative phosphorylation, death receptor signaling, neuroinflammation signaling pathway, and estrogen receptor signaling pathways were significantly modified, and the gene modifications in these pathways were associated with the miRNA changes induced by the maternal undernutrition. For example, the upregulated (P<.05) oxidative phosphorylation pathway in R group was validated using RT-qPCR, and the correlational analysis indicated that miR-221, 103, 107, 184, and 4497 correlate with their target genes NDUFA1, NDUFA11, NDUFB10 and NDUFS7 in this pathway. These results provide the framework for further understanding maternal malnutrition's negative impacts on hepatic metabolic pathways via miRNA-mRNA interactions in full-term fetal pigs.}, journal={JOURNAL OF NUTRITIONAL BIOCHEMISTRY}, author={Wang, Feng and Man, Chaolai and Wang, Xiaoqiu and Odle, Jack and Maltecca, Christian and Lin, Xi}, year={2023}, month={Jun} }
 @article{boston_wang_lin_leonard_kim_mckilligan_fellner_odle_2022, title={Gruel Creep Feeding Accelerates Growth and Alters Intestinal Health of Young Pigs}, volume={12}, ISSN={["2076-2615"]}, url={https://doi.org/10.3390/ani12182408}, DOI={10.3390/ani12182408}, abstractNote={To combat the stress of weaning, we utilized novel gruel creep feeders to supplement suckling pigs with divergent soluble (n = 6 litters) versus insoluble (n = 6) diets compared with un-supplemented controls (n = 6). Post-weaning, pigs were fed a common phase 1 diet. Average daily weight gains of pigs fed soluble and insoluble creep diets were 53% and 17% greater than control pigs, respectively (p < 0.01). Creep intake was higher (82%) for pigs fed the soluble diet, and the accompanying weight increase was sustained post-weaning (p < 0.02). Villus measures were prematurely altered in soluble-creep-fed pigs (p < 0.01), with decreases in villi length, crypt depth, and villus area pre-weaning. No effects of treatment were detected for VFA concentrations and pH in the cecum. There was an interaction between treatment and age for several pro- and anti-inflammatory cytokines (p < 0.01), where soluble-creep-fed pigs had increased cytokine levels with age, whereas cytokine levels in the insoluble and control groups decreased over time. We conclude that a soluble creep diet fed in a gruel state during the pre-weaning period has a positive impact on weaning weight that is sustained post-weaning, and is accompanied by alterations in the intestinal health of young pigs.}, number={18}, journal={ANIMALS}, author={Boston, Timothy E. and Wang, Feng and Lin, Xi and Leonard, Suzanne and Kim, Sung Woo and McKilligan, Denny and Fellner, Vivek and Odle, Jack}, year={2022}, month={Sep} }
 @article{keerthisinghe_wang_wang_yang_li_yang_xi_dong_fang_2020, title={Long-term exposure to TET increases body weight of juvenile zebrafish as indicated in host metabolism and gut microbiome}, volume={139}, ISSN={0160-4120}, url={http://dx.doi.org/10.1016/j.envint.2020.105705}, DOI={10.1016/j.envint.2020.105705}, abstractNote={The application of tetracycline (TET) is very common in medical treatment, fisheries, and animal husbandry, resulting in its frequent detection with abundant concentrations in the aquatic environment. Though the effects of TET on zebrafish (Danio rerio) at embryonic and larval stages have been reported, there is very limited information on the possible long-term effect on aquatic fishes at the juvenile stage, especially at environmentally relevant levels. In this study, we have exposed juvenile zebrafish to two levels of TET at 1 and 100 µg/L for one month until their adulthood. The result showed that both levels of TET can significantly increase the body weight of the zebrafish, while there is no change in the body length. TET exposure also affected the liver microstructure by lipid vacuoles generation and global lipidomics analysis revealed a significant upregulation in hepatic triglyceride (TAG) levels. The metabolomics analysis showed great dysregulations in hepatic metabolic pathways including linoleic acid metabolism, tyrosine metabolism, and methionine metabolism, which are known to be linked with increased body weight gain through hepatic lipid accumulation. The hepatic gene expression involved in lipid transport (e.g., apoa4 and fabp11) and lipogenic factors (e.g., ppar) have been significantly upregulated in the livers of TET exposed zebrafish. Interestingly, the 16 rRNA gene sequence-based zebrafish gut microbial community analysis revealed an enhanced community diversity and altered microbial community composition upon TET exposure. To our knowledge, this is the first study showing that TET exposure can increase the body weight in juvenile zebrafish and the study on the ecotoxicity of antibiotic occurrences in the aquatic system can be further warranted.}, journal={Environment International}, publisher={Elsevier BV}, author={Keerthisinghe, Tharushi Prabha and Wang, Feng and Wang, Mengjing and Yang, Qin and Li, Jiawei and Yang, Jingfeng and Xi, Lin and Dong, Wu and Fang, Mingliang}, year={2020}, month={Jun}, pages={105705} }