Gary Gilleskie

Works (25)

Updated: April 23rd, 2025 19:54

2024 journal article

Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins

Journal of Chromatography A, 1734, 465320.

By: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, G. Gilleskie n  ...

Contributors: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, . al.

author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
MeSH headings : Dependovirus / isolation & purification; Dependovirus / genetics; Dependovirus / chemistry; Chromatography, Affinity / methods; Peptides / chemistry; Peptides / isolation & purification; Serogroup; Humans; Hydrogen-Ion Concentration; Genetic Vectors; HEK293 Cells
topics (OpenAlex): Virus-based gene therapy research; RNA Interference and Gene Delivery; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries, Crossref
Added: September 1, 2024

2023 journal article

Development and Delivery of a Hands-On Short Course in Adeno-Associated Virus Manufacturing to Support Growing Workforce Needs in Gene Therapy

Human Gene Therapy, 34(7-8), 259–272.

By: L. Overton n, C. Boi n , S. Shastry n, C. Smith-Moore n, J. Balchunas n, D. Sambandan n, G. Gilleskie n 

author keywords: viral vectors; AAV; gene therapy; biomanufacturing; training; short course
MeSH headings : Humans; Dependovirus / genetics; Genetic Therapy; Commerce; Genetic Vectors / genetics
topics (OpenAlex): Biomedical and Engineering Education; Viral Infectious Diseases and Gene Expression in Insects; CAR-T cell therapy research
TL;DR: The design of the course, the backgrounds of the nearly 80 students who have participated in the seven offerings held since March 2019, and feedback from the course participants are discussed. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science, NC State University Libraries, Crossref
Added: April 19, 2023

2023 chapter

Digital Twins in Pilot Scale Fermentation: Non-Linear State Estimation for Improving Induction Timing

In Computer Aided Chemical Engineering (pp. 2637–2642).

By: M. Stevnsborg *, K. Selle  n, R. Barton  n, O. Prado-Rubio *, C. Gargalo *, K. Gernaey *, G. Gilleskie n , J. Huusom *

topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Advanced Control Systems Optimization; Fault Detection and Control Systems
Sources: ORCID, Crossref, NC State University Libraries
Added: September 6, 2023

2023 article

Peptide ligands for the affinity purification of adeno-associated viruses from HEK 293 cell lysates

Chu, W., Shastry, S., Barbieri, E., Prodromou, R., Greback-Clarke, P., Smith, W., … Menegatti, S. (2023, February 21). (Vol. 2). Vol. 2.

By: W. Chu  n, S. Shastry  n, E. Barbieri  n, R. Prodromou n, P. Greback-Clarke n, W. Smith  n, B. Moore  n, R. Kilgore  n ...

topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Viral Infections and Immunology Research
Source: ORCID
Added: February 22, 2023

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2023 article

Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography

Shastry, S., WENNING, C. H. U., Barbieri, E., Greback-Clarke, P., Smith, W., Cummings, C., … Menegatti, S. (2023, May 23).

By: S. Shastry  n, C. Wenning  n, E. Barbieri  n, P. Greback-Clarke n, W. Smith  n, C. Cummings  n, A. Minzoni n, J. Pancorbo n ...

topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; CAR-T cell therapy research
Source: ORCID
Added: May 23, 2023

2023 journal article

Rational design and experimental evaluation of peptide ligands for the purification of adeno‐associated viruses via affinity chromatography

Biotechnology Journal, 19(1).

By: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback‐Clarke n, W. Smith  n, C. Cummings n, A. Minzoni n, J. Pancorbo n ...

Contributors: S. Shastry n, W. Chu n, E. Barbieri n, P. Greback-Clarke n, W. Smith  n, C. Cummings n, A. Minzoni n, . al.

author keywords: adeno-associated virus; affinity chromatography; gene therapy; peptide ligands; transduction activity
MeSH headings : Humans; Dependovirus / genetics; Capsid / chemistry; HEK293 Cells; Transduction, Genetic; Peptides / metabolism; Ligands; Chromatography, Affinity; Genetic Vectors / genetics
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; CAR-T cell therapy research
TL;DR: This study introduces a cohort of peptide ligands that mimic the biorecognition activity of the AAV receptor (AAVR) and anti-AAV antibody A20, enable product elution under near-physiological conditions (pH 6.0) and grant extended reusability by withstanding multiple regenerations. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 10, 2023

2022 article

Corrigendum to ‘A direct comparison between membrane adsorber and packed column chromatography performance’

Boi, C., Malavasi, A., Carbonell, R. G., & Gilleskie, G. (2022, February 3). Corrigendum to ‘A direct comparison between membrane adsorber and packed column chromatography performance.’ Journal of Chromatography A, Vol. 3.

By: C. Boi* , A. Malavasi n, R. Carbonell n & G. Gilleskie n 

topics (OpenAlex): Analytical Chemistry and Chromatography; Protein purification and stability
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: February 21, 2022

2022 article

Mobile On Demand COVID-19 Vaccine Production Units for Developing Countries

Fahr, S., Peña-Benavides, S. A., Thiel, L., Sengoba, C., Karacasulu, K., Ihling, N., … Roh, K. (2022, August 19). Industrial & Engineering Chemistry Research, Vol. 61, pp. 13191–13204.

By: S. Fahr *, S. Peña-Benavides *, L. Thiel *, C. Sengoba*, K. Karacasulu *, N. Ihling *, J. Sosa-Hernández *, G. Gilleskie n  ...

Contributors: S. Fahr *, S. Pena-Benavides *, L. Thiel *, C. Sengoba*, K. Karacasulu *, N. Ihling *, J. Sosa-Hernandez *, G. Gilleskie n  ...

topics (OpenAlex): SARS-CoV-2 and COVID-19 Research; SARS-CoV-2 detection and testing; Innovation and Socioeconomic Development
TL;DR: This work designed two MOD vaccine manufacturing units based on a protein antigen expressed in yeast and in vitro transcription of mRNA that can produce on the order of 10,000 vaccine doses daily for competitive prices and in close proximity of their end users. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 14, 2022

2021 book

Biopharmaceutical Manufacturing

In De Gruyter.

By: G. Gilleskie* , C. Rutter & B. McCuen

Contributors: G. Gilleskie* , C. Rutter & B. McCuen

topics (OpenAlex): Biosimilars and Bioanalytical Methods
Sources: ORCID, Crossref, NC State University Libraries
Added: October 16, 2022

2020 article

Recycled eggshells as precursors for iron-impregnated calcium oxide catalysts for partial oxidation of methane

Karoshi, G., Kolar, P., Shah, S. B., & Gilleskie, G. (2020, August 17). Bioresources and Bioprocessing, Vol. 7.

By: G. Karoshi n, P. Kolar n, S. Shah n  & G. Gilleskie n 

author keywords: Eggshell; Iron; Methane; Catalyst; Partial oxidation
topics (OpenAlex): Chemical Looping and Thermochemical Processes; Catalytic Processes in Materials Science; Catalysts for Methane Reforming
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2020

2019 journal article

A direct comparison between membrane adsorber and packed column chromatography performance

Journal of Chromatography A, 1612, 460629.

By: C. Boi* , A. Malavasi n, R. Carbonell n & G. Gilleskie n 

author keywords: Chromatography; Membrane adsorber; Packed column; Binding capacity; Productivity
MeSH headings : Adsorption; Anion Exchange Resins; Chromatography, Ion Exchange / methods; Proteins / isolation & purification; Serum Albumin, Bovine / isolation & purification
topics (OpenAlex): Protein purification and stability; Microfluidic and Capillary Electrophoresis Applications; Analytical Chemistry and Chromatography
TL;DR: The comparison was performed using anion exchange media with the same ligand immobilized on the adsorbing surface, namely the strong Q quaternary ammonium group, R-CH2-N+-(CH3)3, and bovine serum albumin as a model protein to confirm the better performance of membrane chromatography at high flow rates. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: March 10, 2020

2019 article

Valorization of Eggshell Waste into Supported Copper Catalysts for Partial Oxidation of Methane

Karoshi, G., Kolar, P., Shah, S. B., & Gilleskie, G. (2019, November 27). International Journal of Environmental Research.

By: G. Karoshi n, P. Kolar n, S. Shah n  & G. Gilleskie n 

author keywords: Eggshell; Copper; Catalyst; Methane; Partial oxidation
topics (OpenAlex): Catalytic Processes in Materials Science; Catalysts for Methane Reforming; Catalysis and Oxidation Reactions
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 9, 2019

2016 journal article

A Life Cycle Approach to Education and Training for the Biopharmaceutical Industry

Chemical Engineering Education. https://journals.flvc.org/cee/article/view/88298

Contributors: G. Gilleskie , B. Reeves, J. Zanten, J. Balchunas & R. Carbonell

journals.flvc.org
Source: ORCID
Added: October 16, 2022

2016 magazine article

Process-oriented Training in Single-use Technology

BioProcess International Supplement Series.

Contributors: G. Gilleskie 

Source: ORCID
Added: October 16, 2022

2015 article

Calcined eggshell as an inexpensive catalyst for partial oxidation of methane

Karoshi, G., Kolar, P., Shah, S. B., Gilleskie, G., & Das, L. (2015, June 18). Journal of the Taiwan Institute of Chemical Engineers, Vol. 57, pp. 123–128.

By: G. Karoshi n, P. Kolar n, S. Shah n , G. Gilleskie n  & L. Das n

author keywords: Catalyst; Eggshell; Hydrocarbons; Methane oxidation
topics (OpenAlex): Catalytic Processes in Materials Science; Industrial Gas Emission Control; Catalysis and Oxidation Reactions
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 magazine article

Comparison of Concentration Measurement Technologies in Bioprocess Solutions

BioProcess International.

Contributors: M. Johnson, K. Golden, G. Gilleskie , J. Pancorbo & R. Vilhena

Source: ORCID
Added: October 16, 2022

2015 magazine article

Comprehensive Hands-on Training for Biopharmaceutical Manufacturing

BioProcess International.

Contributors: G. Gilleskie  & P. Brown

Source: ORCID
Added: October 16, 2022

2014 article

Comprehensive hands‐on training for influenza vaccine manufacturing: A WHO–BARDA–BTEC partnership for global workforce development

Ruiz, J., Gilleskie, G. L., Brown, P., Burnett, B., & Carbonell, R. G. (2014, August 18). Biochemistry and Molecular Biology Education, Vol. 42, pp. 414–419.

By: J. Ruiz n, G. Gilleskie n , P. Brown n, B. Burnett* & R. Carbonell n

author keywords: workforce development; developing countries vaccine manufacturers; influenza vaccine; global vaccine capacity-building; active learning; cGMP manufacturing
MeSH headings : Capacity Building / methods; Education, Continuing / methods; Humans; Influenza Vaccines / biosynthesis; Influenza Vaccines / immunology; Internet; Program Development / methods; Reproducibility of Results; Technology, Pharmaceutical / education; Technology, Pharmaceutical / methods; United States; United States Dept. of Health and Human Services; World Health Organization
topics (OpenAlex): Influenza Virus Research Studies; vaccines and immunoinformatics approaches; Vaccine Coverage and Hesitancy
TL;DR: The Biomanufacturing Training and Education Center at North Carolina State University has addressed the need for training for staff supporting operations within vaccine production facilities by developing and delivering a comprehensive training program, consisting of three courses: Fundamentals of cGMP Influenza Vaccine Manufacturing, Advanced Upstream Processes for Influenza vaccine Manufacturing, and Advanced Downstream processeses for influenza vaccine Manufacturing. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

From Process Development to Manufacturing: Lab-intensive Courses in Downstream Bioprocessing

Chemical Engineering Education. https://journals.flvc.org/cee/article/view/83365

Contributors: G. Gilleskie  & B. Reeves

journals.flvc.org
Source: ORCID
Added: October 16, 2022

2014 article

Value-addition of methane via selective catalytic oxidation

Karoshi, G., Kolar, P., Shah, S. B., & Gilleskie, G. (2014, March 1). Biofuels, Vol. 5, pp. 175–188.

By: G. Karoshi n, P. Kolar  n, S. Shah  n & G. Gilleskie n 

Contributors: G. Karoshi n, P. Kolar  n, S. Shah  n & G. Gilleskie n 

topics (OpenAlex): Catalysis and Oxidation Reactions; Catalytic Processes in Materials Science; Oxidative Organic Chemistry Reactions
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Source: ORCID
Added: October 16, 2022

2013 magazine article

Biopharmaceutical Microbial Contamination Control

Pharmaceutical Processing.

Contributors: L. Clontz, G. Gilleskie  & N. Hentz

Source: ORCID
Added: October 16, 2022

1995 article

Gas separations in hollow‐fiber adsorbers

Gilleskie, G. L., Parker, J. L., & Cussler, E. L. (1995, June 1). AIChE Journal, Vol. 41, pp. 1413–1425.

By: G. Gilleskie* , J. Parker * & E. Cussler *

Contributors: G. Gilleskie* , J. Parker * & E. Cussler *

topics (OpenAlex): Quantum, superfluid, helium dynamics; Carbon Dioxide Capture Technologies; Spacecraft and Cryogenic Technologies
Source: ORCID
Added: October 16, 2022

1992 article

Pressure Swing Adsorption in Hollow Fibers Immersed in Liquid

Separations Technology.

Contributors: G. Gilleskie  & E. Cussler

Source: ORCID
Added: October 16, 2022

1989 article

Segregation and chemical conversion at oil-water interface

Ganesan, S., Lloyd, P. B., Gilleskie, G. L., & Lim, P. K. (1989, January 1). Chemical Engineering Science, Vol. 44, pp. 171–177.

By: S. Ganesan n, P. Lloyd n, G. Gilleskie n  & P. Lim n

Contributors: S. Ganesan n, P. Lloyd n, G. Gilleskie n  & P. Lim n

topics (OpenAlex): Pickering emulsions and particle stabilization; Enhanced Oil Recovery Techniques; Minerals Flotation and Separation Techniques
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (Web of Science; OpenAlex)
Sources: ORCID, Web Of Science
Added: August 6, 2018

Employment

Updated: October 14th, 2022 08:05

2019 - present

North Carolina State University Raleigh, North Carolina, US
Executive Director Biomanufacturing Training and Education Center

Education

Updated: October 14th, 2022 08:07

1987 - 1992

University of Minnesota Minneapolis, MN, US
PhD Chemical Engineering

1982 - 1986

North Carolina State University Raleigh, North Carolina, US
BS Chemical Engineering

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