Hsi-Chuan Chen

Works (9)

Updated: August 18th, 2023 21:18

2021 article

Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa

Lin, C.-Y., Sun, Y., Song, J., Chen, H.-C., Shi, R., Yang, C., … Chiang, V. L. (2021, October 6). Frontiers in Plant Science, Vol. 12.

By: C. Lin n, Y. Sun *, J. Song n, H. Chen n, R. Shi n, C. Yang n, J. Liu  n, S. Tunlaya-Anukit n ...

Contributors: C. Lin n, H. Chen n, R. Shi n, C. Yang n, J. Liu  n, S. Tunlaya-Anukit n, Y. Lin n, R. Sederoff n ...

author keywords: protein interaction; monolignol biosynthesis; wood formation; Populus trichocarpa; BiFC; metabolic flux
topics (OpenAlex): Plant Gene Expression Analysis; Lignin and Wood Chemistry; Biofuel production and bioconversion
TL;DR: It is proposed that Ptr4CL/PtrHCT complexes modulate the metabolic flux of CoA ligation for monolignol biosynthesis during wood formation in P. trichocarpa. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 8, 2021

2015 article

4-Coumaroyl and Caffeoyl Shikimic Acids Inhibit 4-Coumaric Acid:Coenzyme A Ligases and Modulate Metabolic Flux for 3-Hydroxylation in Monolignol Biosynthesis of Populus trichocarpa

Lin, C.-Y., Wang, J. P., Li, Q., Chen, H.-C., Liu, J., Loziuk, P., … Chiang, V. L. (2015, January 1). Molecular Plant, Vol. 8, pp. 176–187.

By: C. Lin n, J. Wang n , Q. Li *, H. Chen n, J. Liu  n, P. Loziuk n, J. Song  n, C. Williams n  ...

Contributors: C. Lin n, J. Wang n , Q. Li *, H. Chen n, J. Liu  n, P. Loziuk n, J. Song  n, C. Williams n  ...

author keywords: monolignol biosynthesis; Populus trichocarpa; metabolic flux; reaction and inhibition kinetics; LC-MS/MS; 4-coumaroyl and caffeoyl shikimic acids
MeSH headings : Acyl Coenzyme A / metabolism; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Hydroxylation / drug effects; Plant Proteins / metabolism; Populus / drug effects; Populus / enzymology; Populus / metabolism; Propionates; Shikimic Acid / pharmacology
topics (OpenAlex): Biochemical and biochemical processes; Plant Gene Expression Analysis; Lignin and Wood Chemistry
TL;DR: 4-coumaroyl and caffeoyl shikimic acids are inhibitors of Ptr4CL3 and Ptr 4CL5, which can reduce lignin content in a number of plant species and play significant regulatory roles when these inhibitors accumulate. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 article

A simple improved-throughput xylem protoplast system for studying wood formation

Lin, Y.-C., Li, W., Chen, H., Li, Q., Sun, Y.-H., Shi, R., … Chiang, V. L. (2014, August 21). Nature Protocols, Vol. 9, pp. 2194–2205.

By: Y. Lin n, W. Li n, H. Chen n, Q. Li n, Y. Sun *, R. Shi n, C. Lin n, J. Wang n  ...

Contributors: Y. Lin n, W. Li n, H. Chen n, Q. Li n, Y. Sun *, R. Shi n, C. Lin n, J. Wang n  ...

MeSH headings : Cell Wall / metabolism; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks / genetics; Green Fluorescent Proteins; Populus / cytology; Populus / growth & development; Protoplasts / metabolism; Transfection / methods; Wood / cytology; Wood / growth & development; Xylem / chemistry
topics (OpenAlex): Plant Molecular Biology Research; Plant Reproductive Biology; Plant Gene Expression Analysis
TL;DR: A protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa is presented. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein Complex Formation, Regulation, and Numerical Modeling

The Plant Cell, 26(3), 876–893.

By: H. Chen n, J. Song  n, J. Wang n , Y. Lin  n, J. Ducoste n , C. Shuford n, J. Liu  n, Q. Li n ...

Contributors: H. Chen n, J. Song  n, J. Wang n , Y. Lin  n, J. Ducoste n , C. Shuford n, J. Liu  n, Q. Li n ...

MeSH headings : Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Immunoprecipitation; Lignin / biosynthesis; Mass Spectrometry; Models, Biological; Populus / metabolism; Propionates; RNA, Messenger / genetics; Substrate Specificity; Systems Biology
topics (OpenAlex): Biofuel production and bioconversion; Lignin and Wood Chemistry; Plant Gene Expression Analysis; Biochemical and biochemical processes
TL;DR: This work shows that 4CL, an enzyme in monolignol biosynthesis, is found as a heterotetrameric complex of two isoforms in Populus trichocarpa, where the two 4-coumaric acid:CoA ligase (4CL) isoforms, 4CL3 and 4CL5, interact in vivo and in vitro to form a heterosymmetric protein complex. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2013 article

Monolignol Pathway 4-Coumaric Acid:Coenzyme A Ligases in Populus. trichocarpa: Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes 

Chen, H.-C., Song, J., Williams, C. M., Shuford, C. M., Liu, J., Wang, J. P., … Chiang, V. L. (2013, January 23). PLANT PHYSIOLOGY, Vol. 161, pp. 1501–1516.

By: H. Chen n, J. Song n, C. Williams n , C. Shuford n, J. Liu  n, J. Wang n , Q. Li n, R. Shi n ...

Contributors: H. Chen n, J. Song n, C. Williams n , C. Shuford n, J. Liu  n, J. Wang n , Q. Li n, R. Shi n ...

MeSH headings : Allosteric Regulation / drug effects; Binding Sites; Biosynthetic Pathways / drug effects; Blotting, Western; Caffeic Acids / pharmacology; Coenzyme A / metabolism; Coenzyme A Ligases / antagonists & inhibitors; Coenzyme A Ligases / metabolism; Computer Simulation; Coumaric Acids / chemistry; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Kinetics; Lignin / biosynthesis; Lignin / chemistry; Phenylpropionates / metabolism; Phosphoproteins / metabolism; Phosphorylation / drug effects; Plant Extracts; Populus / drug effects; Populus / enzymology; Propionates; Proteomics; Recombinant Fusion Proteins / metabolism; Sequence Homology, Amino Acid; Substrate Specificity / drug effects; Xylem / drug effects; Xylem / metabolism
topics (OpenAlex): Biochemical and biochemical processes; Plant biochemistry and biosynthesis; Plant Gene Expression Analysis
TL;DR: Evidence is presented of two monolignol 4CLs, Ptr4CL3 and P tr4CL5, in Populus trichocarpa that act to mitigate the inhibition by caffeic acid to maintain a proper ligation flux and coenzyme A flux with novel specificity. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 article

Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood forming tissue of Populus trichocarpa

Shi, R., Shuford, C. M., Wang, J. P., Sun, Y.-H., Yang, Z., Chen, H.-C., … Chiang, V. L. (2013, June 13). Planta, Vol. 238, pp. 487–497.

By: R. Shi n, C. Shuford n, J. Wang n , Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n ...

Contributors: R. Shi n, C. Shuford n, J. Wang n , Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n ...

MeSH headings : Gene Expression Regulation, Plant / physiology; Mass Spectrometry; Phenylalanine Ammonia-Lyase / metabolism; Populus / enzymology; Populus / metabolism
topics (OpenAlex): Plant Gene Expression Analysis; Plant biochemistry and biosynthesis; Horticultural and Viticultural Research
TL;DR: A greater functional significance of subgroup A PtrPALs for stem wood formation is indicated, and the role of gene post-transcriptional regulation is highlighted. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Comprehensive Quantification of Monolignol-Pathway Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution Mass Spectrometry

Journal of Proteome Research, 11(6), 3390–3404.

By: C. Shuford  n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n , R. Shi n, R. Sederoff n, V. Chiang n, D. Muddiman n 

Contributors: C. Shuford  n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n , R. Shi n, R. Sederoff n, V. Chiang n, D. Muddiman n 

author keywords: quantification; isotope dilution mass spectrometry; selected reaction monitoring; filter-aided sample preparation; monolignol biosynthesis
MeSH headings : Amino Acid Sequence; Biosynthetic Pathways; Isotope Labeling; Molecular Sequence Data; Plant Proteins / chemistry; Plant Proteins / metabolism; Populus / enzymology; Proteome / chemistry; Proteome / metabolism; Reference Standards; Spectrometry, Mass, Electrospray Ionization / standards; Xylem / enzymology
topics (OpenAlex): Fermentation and Sensory Analysis; Plant Gene Expression Analysis; Biochemical and biochemical processes
TL;DR: This represents the most comprehensive assay for quantification of proteins regulating monolignol biosynthesis and will lead to a better understanding of lignin formation at a systems level. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2012 article

Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC–MS/MS based protein quantification and metabolic flux analysis

Wang, J. P., Shuford, C. M., Li, Q., Song, J., Lin, Y.-C., Sun, Y.-H., … Chiang, V. L. (2012, May 24). Planta, Vol. 236, p. 795–808.

By: J. Wang n , C. Shuford n, Q. Li n, J. Song  n, Y. Lin n, Y. Sun n, H. Chen n, C. Williams n  ...

Contributors: J. Wang n , C. Shuford n, Q. Li n, J. Song  n, Y. Lin n, Y. Sun n, H. Chen n, C. Williams n  ...

author keywords: Lignin; Syringyl lignin; Angiosperm; Kinetics; Subcellular localization
MeSH headings : Cloning, Molecular; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Hydroxylation; Kinetics; Lignin / analysis; Lignin / biosynthesis; Mixed Function Oxygenases / metabolism; Plants, Genetically Modified; Populus / metabolism; Xylem / enzymology; Yeasts / metabolism
topics (OpenAlex): Plant Gene Expression Analysis; Fermentation and Sensory Analysis; Biochemical and biochemical processes
TL;DR: The combination of genetic analysis, absolute protein quantitation-based enzyme kinetics, homologous CPR specificity, SNP characterization, and ER localization provides a more rigorous basis for a comprehensive systems understanding of 5-hydroxylation in lignin biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2011 article

Membrane protein complexes catalyze both 4- and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis

Chen, H.-C., Li, Q., Shuford, C. M., Liu, J., Muddiman, D. C., Sederoff, R. R., & Chiang, V. L. (2011, December 12). Proceedings of the National Academy of Sciences, Vol. 108, pp. 21253–21258.

By: H. Chen*, Q. Li*, C. Shuford n, J. Liu, D. Muddiman n , R. Sederoff*, V. Chiang*

MeSH headings : Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / metabolism; Chromatography, Liquid; Coumaric Acids; DNA Primers / genetics; Hydroxylation; Immunoprecipitation; Kinetics; Lignin / biosynthesis; Mass Spectrometry; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Microscopy, Confocal; Molecular Structure; Multiprotein Complexes / chemistry; Multiprotein Complexes / metabolism; Phenols; Phenylpropionates; Plasmids / genetics; Populus / metabolism; Propionates; Trans-Cinnamate 4-Monooxygenase / chemistry; Trans-Cinnamate 4-Monooxygenase / metabolism; Xylem / metabolism; Yeasts
topics (OpenAlex): Plant Gene Expression Analysis; Microbial metabolism and enzyme function; Carbohydrate Chemistry and Synthesis
TL;DR: Affinity purification-quantitative mass spectrometry, bimolecular fluorescence complementation, chemical cross-linking, and reciprocal coimmunoprecipitation provide further evidence for functional involvement of membrane protein complexes in monolignol biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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