Heath Ellis Johnson

Works (8)

Updated: August 17th, 2023 21:16

2016 article

Are Filopodia Privileged Signaling Structures in Migrating Cells?

Johnson, H. E., & Haugh, J. M. (2016, October 7). Biophysical Journal, Vol. 111, pp. 1827–1830.

By: H. Johnson n & J. Haugh n 

MeSH headings : Animals; Cell Movement; Mice; NIH 3T3 Cells; Optical Imaging; Pseudopodia / metabolism; Signal Transduction
topics (OpenAlex): Cellular Mechanics and Interactions; Cell Image Analysis Techniques; Microtubule and mitosis dynamics
TL;DR: This work analyzed total internal reflection fluorescence images of migrating fibroblasts coexpressing either a plasma membrane marker or tagged AktPH domain, a translocation biosensor for signaling through the phosphoinositide 3-kinase pathway, along with a cytosolic volume marker to support the filopodial signaling hypothesis. (via Semantic Scholar)
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Added: August 6, 2018

2016 article

Lamellipodia are crucial for haptotactic sensing and response

King, S. J., Asokan, S. B., Haynes, E. M., Zimmerman, S. P., Rotty, J. D., Alb, J. G., … Bear, J. E. (2016, May 13). Journal of Cell Science, Vol. 129, pp. 2329–2342.

By: S. King*, S. Asokan*, E. Haynes *, S. Zimmerman*, J. Rotty *, J. Alb*, A. Tagliatela*, D. Blake * ...

author keywords: Haptotaxis; Arp2/3; Lamellipodia; Directed migration
MeSH headings : Actin-Related Protein 2-3 Complex / metabolism; Actins / metabolism; Animals; Chemotaxis / drug effects; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Fibronectins / pharmacology; Focal Adhesion Protein-Tyrosine Kinases / metabolism; Focal Adhesions / metabolism; Guanine Nucleotide Exchange Factors / metabolism; Integrin beta1 / metabolism; Mice; Models, Biological; Pseudopodia / metabolism; Signal Transduction / drug effects; T-Lymphoma Invasion and Metastasis-inducing Protein 1; Wiskott-Aldrich Syndrome Protein / metabolism; Wiskott-Aldrich Syndrome Protein Family / metabolism; rac GTP-Binding Proteins / metabolism; src-Family Kinases / metabolism
topics (OpenAlex): Cellular Mechanics and Interactions; Marine Biology and Environmental Chemistry; Planarian Biology and Electrostimulation
TL;DR: Haptotaxis (directed migration) on a substrate-bound gradient is perhaps the least-well understood form of directed migration and it is shown that differential lamellipodial dynamics are crucial for this process. (via Semantic Scholar)
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Added: August 6, 2018

2015 article

F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling

Johnson, H. E., King, S. J., Asokan, S. B., Rotty, J. D., Bear, J. E., & Haugh, J. M. (2015, February 9). The Journal of Cell Biology, Vol. 208, pp. 443–455.

By: H. Johnson n, S. King*, S. Asokan*, J. Rotty *, J. Bear * & J. Haugh n 

MeSH headings : 3T3 Cells; Actin Cytoskeleton; Actin-Related Protein 2-3 Complex / genetics; Actin-Related Protein 2-3 Complex / metabolism; Actins / physiology; Animals; Cell Adhesion / physiology; Cell Line; Cell Shape; Chemotaxis / genetics; Chemotaxis / physiology; Fibroblasts / cytology; Fibronectins / metabolism; Focal Adhesion Protein-Tyrosine Kinases / metabolism; Mice; Microfilament Proteins / biosynthesis; Microfilament Proteins / genetics; Microscopy, Fluorescence; Molecular Sequence Data; Phosphatidylinositol 3-Kinases / metabolism; Platelet-Derived Growth Factor / metabolism; Pseudopodia / physiology; RNA Interference; RNA, Small Interfering; Receptors, Odorant / biosynthesis; Receptors, Odorant / genetics
topics (OpenAlex): Cellular Mechanics and Interactions; Cell Adhesion Molecules Research; Cancer Cells and Metastasis
TL;DR: During cell migration, F-actin bundles/filopodia serve as templates for formation and orientation of lamellipodia and prime their stabilization by adhesion-based PI3K signaling. (via Semantic Scholar)
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Added: August 6, 2018

2015 article

GMFβ controls branched actin content and lamellipodial retraction in fibroblasts

Haynes, E. M., Asokan, S. B., King, S. J., Johnson, H. E., Haugh, J. M., & Bear, J. E. (2015, June 22). The Journal of Cell Biology, Vol. 209, pp. 803–812.

By: E. Haynes *, S. Asokan*, S. King*, H. Johnson n, J. Haugh n  & J. Bear *

MeSH headings : Actin-Related Protein 2-3 Complex / antagonists & inhibitors; Actin-Related Protein 2-3 Complex / metabolism; Actins / biosynthesis; Animals; Cell Line; Cell Movement / physiology; Enzyme Activation; Fibroblasts / physiology; Fibronectins / pharmacology; Glia Maturation Factor / physiology; Indoles / pharmacology; Mice; Protein Isoforms / biosynthesis; Pseudopodia / physiology
topics (OpenAlex): Cellular Mechanics and Interactions; Cell Adhesion Molecules Research; Viral Infectious Diseases and Gene Expression in Insects
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2014 article

Mesenchymal Chemotaxis Requires Selective Inactivation of Myosin II at the Leading Edge via a Noncanonical PLCγ/PKCα Pathway

Asokan, S. B., Johnson, H. E., Rahman, A., King, S. J., Rotty, J. D., Lebedeva, I. P., … Bear, J. E. (2014, December 1). Developmental Cell, Vol. 31, pp. 747–760.

By: S. Asokan*, H. Johnson n, A. Rahman n, S. King *, J. Rotty *, I. Lebedeva*, J. Haugh n , J. Bear *

MeSH headings : Animals; Cell Line; Cell Movement; Chemotaxis / physiology; Diglycerides / chemistry; Green Fluorescent Proteins / metabolism; Humans; Ligands; Mesoderm / physiology; Mice; Mice, Transgenic; Myosin Type II / metabolism; Phorbol Esters; Phospholipase C gamma / metabolism; Phosphorylation; Platelet-Derived Growth Factor / metabolism; Protein Kinase C-alpha / metabolism; Signal Transduction
topics (OpenAlex): Cell Adhesion Molecules Research; Cellular Mechanics and Interactions; Tissue Engineering and Regenerative Medicine
TL;DR: It is demonstrated that local inactivation of Myosin IIA, through a noncanonical Ser1/2 phosphorylation of the regulatory light chain, is essential for mesenchymal chemotaxis and subsequent inhibition of MyOSin II activity at the leading edge are required. (via Semantic Scholar)
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Added: August 6, 2018

2014 article

Profilin-1 Serves as a Gatekeeper for Actin Assembly by Arp2/3-Dependent and -Independent Pathways

Rotty, J. D., Wu, C., Haynes, E. M., Suarez, C., Winkelman, J. D., Johnson, H. E., … Bear, J. E. (2014, December 24). Developmental Cell, Vol. 32, pp. 54–67.

By: J. Rotty *, C. Wu *, E. Haynes *, C. Suarez *, J. Winkelman *, H. Johnson n, J. Haugh n , D. Kovar *, J. Bear *

MeSH headings : Actin Cytoskeleton / metabolism; Actin-Related Protein 2-3 Complex / metabolism; Actins / metabolism; Animals; Female; Fetal Proteins; Fibroblasts / cytology; Formins; Image Processing, Computer-Assisted; Male; Mice; Mice, Inbred C57BL; Microfilament Proteins; Microscopy, Fluorescence; Nuclear Proteins; Profilins / metabolism; Signal Transduction; Stress Fibers
topics (OpenAlex): Cellular Mechanics and Interactions; Cell Adhesion Molecules Research; Cardiomyopathy and Myosin Studies
TL;DR: Antagonism of the Arp2/3 complex by profilin-1 in cells appears to maintain actin homeostasis by balancing Arp1/2 complex-dependent and -independent actin assembly pathways. (via Semantic Scholar)
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Added: August 6, 2018

2013 article

Bidirectional coupling between integrin-mediated signaling and actomyosin mechanics explains matrix-dependent intermittency of leading-edge motility

Welf, E. S., Johnson, H. E., & Haugh, J. M. (2013, October 23). Molecular Biology of the Cell, Vol. 24, pp. 3945–3955.

By: E. Welf n, H. Johnson n & J. Haugh n 

MeSH headings : Actin Cytoskeleton; Actins / metabolism; Actomyosin / physiology; Animals; Cell Adhesion / physiology; Cell Adhesion Molecules / genetics; Cell Movement / physiology; Computer Simulation; Extracellular Matrix / metabolism; Focal Adhesions / physiology; Integrins / genetics; Integrins / metabolism; Integrins / physiology; Mechanotransduction, Cellular; Myosin Type II / metabolism; Pseudopodia / metabolism; Signal Transduction / genetics; rhoA GTP-Binding Protein / metabolism
topics (OpenAlex): Cellular Mechanics and Interactions; 3D Printing in Biomedical Research; Force Microscopy Techniques and Applications
TL;DR: A physicochemical model is used to describe the coupling of adhesion, cytoskeletal, and signaling dynamics during cell migration, and analysis of stochastic simulations predicts relationships between measurable quantities that reflect partitioning of stress between F-actin–bound adhesions. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 article

Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism

Welf, E. S., Ahmed, S., Johnson, H. E., Melvin, A. T., & Haugh, J. M. (2012, April 2). The Journal of Cell Biology.

By: E. Welf n, S. Ahmed n, H. Johnson n, A. Melvin n & J. Haugh n 

MeSH headings : Animals; Cell Movement; Cells, Cultured; Fibroblasts / cytology; Fibroblasts / enzymology; Fibroblasts / metabolism; Mice; NIH 3T3 Cells; Phosphatidylinositol 3-Kinases / metabolism; Phosphoinositide-3 Kinase Inhibitors; Signal Transduction / drug effects
topics (OpenAlex): Cellular Mechanics and Interactions; Cancer Cells and Metastasis; Microtubule and mitosis dynamics
TL;DR: Migrating fibroblasts reorient directionality by PI3K-dependent branching and pivoting of protrusions, a mechanism that allows fibro Blasts to align with an external chemotactic gradient. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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