Works (8)

Updated: August 17th, 2023 21:16

2016 journal article

Are Filopodia Privileged Signaling Structures in Migrating Cells?

BIOPHYSICAL JOURNAL, 111(9), 1827–1830.

By: H. Johnson n & J. Haugh n

MeSH headings : Animals; Cell Movement; Mice; NIH 3T3 Cells; Optical Imaging; Pseudopodia / metabolism; Signal Transduction
TL;DR: This work analyzed total internal reflection fluorescence images of migrating fibroblasts coexpressing either a plasma membrane marker or tagged AktPH domain, a translocation biosensor for signaling through the phosphoinositide 3-kinase pathway, along with a cytosolic volume marker to support the filopodial signaling hypothesis. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Lamellipodia are crucial for haptotactic sensing and response

JOURNAL OF CELL SCIENCE, 129(12), 2329–2342.

By: S. King*, S. Asokan*, E. Haynes*, S. Zimmerman*, J. Rotty*, J. Alb*, A. Tagliatela*, D. Blake* ...

author keywords: Haptotaxis; Arp2/3; Lamellipodia; Directed migration
MeSH headings : Actin-Related Protein 2-3 Complex / metabolism; Actins / metabolism; Animals; Chemotaxis / drug effects; Fibroblasts / cytology; Fibroblasts / drug effects; Fibroblasts / metabolism; Fibronectins / pharmacology; Focal Adhesion Protein-Tyrosine Kinases / metabolism; Focal Adhesions / metabolism; Guanine Nucleotide Exchange Factors / metabolism; Integrin beta1 / metabolism; Mice; Models, Biological; Pseudopodia / metabolism; Signal Transduction / drug effects; T-Lymphoma Invasion and Metastasis-inducing Protein 1; Wiskott-Aldrich Syndrome Protein / metabolism; Wiskott-Aldrich Syndrome Protein Family / metabolism; rac GTP-Binding Proteins / metabolism; src-Family Kinases / metabolism
TL;DR: Haptotaxis (directed migration) on a substrate-bound gradient is perhaps the least-well understood form of directed migration and it is shown that differential lamellipodial dynamics are crucial for this process. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling

JOURNAL OF CELL BIOLOGY, 208(4), 443–455.

By: H. Johnson n, S. King*, S. Asokan*, J. Rotty*, J. Bear* & J. Haugh n

MeSH headings : 3T3 Cells; Actin Cytoskeleton; Actin-Related Protein 2-3 Complex / genetics; Actin-Related Protein 2-3 Complex / metabolism; Actins / physiology; Animals; Cell Adhesion / physiology; Cell Line; Cell Shape; Chemotaxis / genetics; Chemotaxis / physiology; Fibroblasts / cytology; Fibronectins / metabolism; Focal Adhesion Protein-Tyrosine Kinases / metabolism; Mice; Microfilament Proteins / biosynthesis; Microfilament Proteins / genetics; Microscopy, Fluorescence; Molecular Sequence Data; Phosphatidylinositol 3-Kinases / metabolism; Platelet-Derived Growth Factor / metabolism; Pseudopodia / physiology; RNA Interference; RNA, Small Interfering; Receptors, Odorant / biosynthesis; Receptors, Odorant / genetics
TL;DR: During cell migration, F-actin bundles/filopodia serve as templates for formation and orientation of lamellipodia and prime their stabilization by adhesion-based PI3K signaling. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

GMF beta controls branched actin content and lamellipodial retraction in fibroblasts

JOURNAL OF CELL BIOLOGY, 209(6), 803–812.

By: E. Haynes*, S. Asokan*, S. King*, H. Johnson n, J. Haugh n & J. Bear*

MeSH headings : Actin-Related Protein 2-3 Complex / antagonists & inhibitors; Actin-Related Protein 2-3 Complex / metabolism; Actins / biosynthesis; Animals; Cell Line; Cell Movement / physiology; Enzyme Activation; Fibroblasts / physiology; Fibronectins / pharmacology; Glia Maturation Factor / physiology; Indoles / pharmacology; Mice; Protein Isoforms / biosynthesis; Pseudopodia / physiology
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Profilin-1 Serves as a Gatekeeper for Actin Assembly by Arp2/3-Dependent and -Independent Pathways

DEVELOPMENTAL CELL, 32(1), 54–67.

MeSH headings : Actin Cytoskeleton / metabolism; Actin-Related Protein 2-3 Complex / metabolism; Actins / metabolism; Animals; Female; Fetal Proteins; Fibroblasts / cytology; Formins; Image Processing, Computer-Assisted; Male; Mice; Mice, Inbred C57BL; Microfilament Proteins; Microscopy, Fluorescence; Nuclear Proteins; Profilins / metabolism; Signal Transduction; Stress Fibers
TL;DR: Antagonism of the Arp2/3 complex by profilin-1 in cells appears to maintain actin homeostasis by balancing Arp1/2 complex-dependent and -independent actin assembly pathways. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Mesenchymal Chemotaxis Requires Selective Inactivation of Myosin II at the Leading Edge via a Noncanonical PLC gamma/PKC alpha Pathway

DEVELOPMENTAL CELL, 31(6), 747–760.

By: S. Asokan*, H. Johnson n, A. Rahman n, S. King*, J. Rotty*, I. Lebedeva*, J. Haugh n, J. Bear*

MeSH headings : Animals; Cell Line; Cell Movement; Chemotaxis / physiology; Diglycerides / chemistry; Green Fluorescent Proteins / metabolism; Humans; Ligands; Mesoderm / physiology; Mice; Mice, Transgenic; Myosin Type II / metabolism; Phorbol Esters; Phospholipase C gamma / metabolism; Phosphorylation; Platelet-Derived Growth Factor / metabolism; Protein Kinase C-alpha / metabolism; Signal Transduction
TL;DR: It is demonstrated that local inactivation of Myosin IIA, through a noncanonical Ser1/2 phosphorylation of the regulatory light chain, is essential for mesenchymal chemotaxis and subsequent inhibition of MyOSin II activity at the leading edge are required. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

Bidirectional coupling between integrin-mediated signaling and actomyosin mechanics explains matrix-dependent intermittency of leading-edge motility

MOLECULAR BIOLOGY OF THE CELL, 24(24), 3945–3955.

By: E. Welf n, H. Johnson n & J. Haugh n

MeSH headings : Actin Cytoskeleton; Actins / metabolism; Actomyosin / physiology; Animals; Cell Adhesion / physiology; Cell Adhesion Molecules / genetics; Cell Movement / physiology; Computer Simulation; Extracellular Matrix / metabolism; Focal Adhesions / physiology; Integrins / genetics; Integrins / metabolism; Integrins / physiology; Mechanotransduction, Cellular; Myosin Type II / metabolism; Pseudopodia / metabolism; Signal Transduction / genetics; rhoA GTP-Binding Protein / metabolism
TL;DR: A physicochemical model is used to describe the coupling of adhesion, cytoskeletal, and signaling dynamics during cell migration, and analysis of stochastic simulations predicts relationships between measurable quantities that reflect partitioning of stress between F-actin–bound adhesions. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism

JOURNAL OF CELL BIOLOGY, 197(1), 105–114.

By: E. Welf n, S. Ahmed n, H. Johnson n, A. Melvin n & J. Haugh n

MeSH headings : Animals; Cell Movement; Cells, Cultured; Fibroblasts / cytology; Fibroblasts / enzymology; Fibroblasts / metabolism; Mice; NIH 3T3 Cells; Phosphatidylinositol 3-Kinases / metabolism; Phosphoinositide-3 Kinase Inhibitors; Signal Transduction / drug effects
TL;DR: Migrating fibroblasts reorient directionality by PI3K-dependent branching and pivoting of protrusions, a mechanism that allows fibro Blasts to align with an external chemotactic gradient. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

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