Hrvoje Lusic

Works (17)

Updated: April 25th, 2025 19:05

2013 article

Cellular Delivery and Photochemical Activation of Antisense Agents through a Nucleobase Caging Strategy

Govan, J. M., Uprety, R., Thomas, M., Lusic, H., Lively, M. O., & Deiters, A. (2013, August 5). ACS Chemical Biology.

By: J. Govan n, R. Uprety n, M. Thomas n, H. Lusic n, M. Lively* & A. Deiters n

MeSH headings : Base Sequence; Gene Transfer Techniques; HeLa Cells; Humans; Light; Models, Biological; Molecular Structure; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / genetics; Oligonucleotides, Antisense / metabolism; Polymerase Chain Reaction; tat Gene Products, Human Immunodeficiency Virus / chemistry; tat Gene Products, Human Immunodeficiency Virus / genetics
topics (OpenAlex): RNA Interference and Gene Delivery; Advanced biosensing and bioanalysis techniques; Photochromic and Fluorescence Chemistry
TL;DR: The conjugation of multiple HIV TAT peptides to a hairpin-protected antisenseAgent through a light-cleavable nucleobase caging group allows for the facile delivery of the antisense agent without a transfection reagent, and photochemical activation offers precise control over gene expression. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 article

Optochemical control of RNA interference in mammalian cells

Govan, J. M., Young, D. D., Lusic, H., Liu, Q., Lively, M. O., & Deiters, A. (2013, September 10). Nucleic Acids Research.

By: J. Govan n, D. Young  n, H. Lusic n, Q. Liu  n, M. Lively n & A. Deiters n

MeSH headings : Benzodioxoles / chemistry; Green Fluorescent Proteins / genetics; Guanosine / chemistry; HEK293 Cells; HeLa Cells; Humans; Kinesins / genetics; Light; Organophosphorus Compounds / chemical synthesis; Organophosphorus Compounds / chemistry; RNA Interference; RNA, Small Interfering / chemistry; RNA, Small Interfering / radiation effects; Uridine / chemistry
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; RNA Interference and Gene Delivery
TL;DR: New photocaged guanosine and uridine RNA phosphoramidites are designed, synthesized and site-specifically incorporated into short RNA duplexes and demonstrated in the light-regulation of the expression of an exogenous green fluorescent protein reporter gene and an endogenous target gene, the mitosis motor protein, Eg5. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2011 journal article

A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos

Development, 139(2), 437–442.

By: A. Morckel n, H. Lusic n, L. Farzana n, J. Yoder n , A. Deiters n & N. Nascone-Yoder n 

author keywords: Small-molecule inhibitor; Rho kinase; Photocaging; Xenopus
MeSH headings : Animals; Body Patterning / drug effects; Dioxoles / metabolism; Dioxoles / pharmacology; Embryo, Nonmammalian / metabolism; Immunohistochemistry; Indoles / metabolism; Indoles / pharmacology; Mice; NIH 3T3 Cells; Pyridines / metabolism; Pyridines / pharmacology; Ultraviolet Rays; Xenopus laevis / embryology; Xenopus laevis / metabolism; rho-Associated Kinases / antagonists & inhibitors; rho-Associated Kinases / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; Click Chemistry and Applications
TL;DR: A novel pharmacological agent that can be light activated to achieve spatiotemporally limited inhibition of Rho kinase activity in vivo is developed and application to the spatially heterogeneous problem of embryonic left-right asymmetry revealed a differential requirement for Rho signaling on the left and right sides of the primitive gut tube. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2010 article

Generating Permissive Site-Specific Unnatural Aminoacyl-tRNA Synthetases

Miyake-Stoner, S. J., Refakis, C. A., Hammill, J. T., Lusic, H., Hazen, J. L., Deiters, A., & Mehl, R. A. (2010, January 18). Biochemistry.

By: S. Miyake-Stoner *, C. Refakis*, J. Hammill *, H. Lusic n, J. Hazen*, A. Deiters n, R. Mehl *

MeSH headings : Amino Acids / chemistry; Amino Acids / metabolism; Amino Acyl-tRNA Synthetases / chemistry; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Fluorine / chemistry; Green Fluorescent Proteins / chemistry; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Structure; Protein Engineering; Substrate Specificity
topics (OpenAlex): RNA and protein synthesis mechanisms; Mass Spectrometry Techniques and Applications; RNA modifications and cancer
TL;DR: Methods for quickly and easily assessing the permissivity of existing UaaRSs and for generating permissive UAARSs are outlined, whose relaxed substrate recognition properties allow them to incorporate multiple unnatural amino acids (but not natural amino acids), are outlined. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2010 journal article

Genetically encoded photocontrol of protein localization in mammalian cells

Journal of the American Chemical Society, 132(12), 4086-.

By: A. Gautier, D. Nguyen, H. Lusic, W. An, A. Deiters & J. Chin

Source: NC State University Libraries
Added: August 6, 2018

2010 article

Improved Synthesis of the Two-Photon Caging Group 3-Nitro-2-Ethyldibenzofuran and Its Application to a Caged Thymidine Phosphoramidite

Lusic, H., Uprety, R., & Deiters, A. (2010, January 29). Organic Letters.

By: H. Lusic n, R. Uprety n & A. Deiters n

MeSH headings : Benzofurans / chemical synthesis; Benzofurans / chemistry; DNA / chemical synthesis; DNA / chemistry; Organophosphorus Compounds / chemistry; Photons; Thymidine Monophosphate / analogs & derivatives; Thymidine Monophosphate / chemistry
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Photoreceptor and optogenetics research; Porphyrin and Phthalocyanine Chemistry
TL;DR: A new and efficient route to the recently reported 3-nitro-2-ethyldibenzofuran caging group was developed and its installation on a thymidine phosphoramidite is described. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2010 journal article

Photocaged Morpholino Oligomers for the Light-Regulation of Gene Function in Zebrafish and Xenopus Embryos

Journal of the American Chemical Society, 132(44), 15644–15650.

By: A. Deiters n, R. Garner n, H. Lusic n, J. Govan n, M. Dush n, N. Nascone-Yoder n , J. Yoder n 

MeSH headings : Animals; Cells, Cultured; Embryo, Nonmammalian / metabolism; Gene Expression; Morpholines / chemistry; Oligonucleotides / genetics; Oligonucleotides / metabolism; Ultraviolet Rays; Xenopus laevis / genetics; Xenopus laevis / metabolism; Zebrafish / genetics; Zebrafish / metabolism
topics (OpenAlex): Photochromic and Fluorescence Chemistry; RNA Interference and Gene Delivery; Advanced biosensing and bioanalysis techniques
TL;DR: A novel and efficient synthetic route for incorporating photocaged monomeric building blocks directly into morpholino oligomers is reported and the utility of these caged morpholinos in the light-activated control of gene function in both cell culture and living embryos is demonstrated. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2010 article

Photocleavable Polyethylene Glycol for the Light-Regulation of Protein Function

Georgianna, W. E., Lusic, H., McIver, A. L., & Deiters, A. (2010, July 22). Bioconjugate Chemistry.

By: W. Georgianna n, H. Lusic n, A. McIver n & A. Deiters n

MeSH headings : Crystallography, X-Ray; Models, Molecular; Molecular Structure; Muramidase / chemistry; Muramidase / metabolism; Photochemical Processes; Polyethylene Glycols / chemical synthesis; Polyethylene Glycols / chemistry; Stereoisomerism; Ultraviolet Rays
topics (OpenAlex): Click Chemistry and Applications; Photochromic and Fluorescence Chemistry; Chemical Synthesis and Analysis
TL;DR: The synthesis of a photocleavable PEG reagent (PhotoPEG) and its application to the light-regulation of enzyme activity is reported. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2010 article

The Human Mitochondrial tRNAMet: Structure/Function Relationship of a Unique Modification in the Decoding of Unconventional Codons

Bilbille, Y., Gustilo, E. M., Harris, K. A., Jones, C. N., Lusic, H., Kaiser, R. J., … Agris, P. F. (2010, December 18). Journal of Molecular Biology.

By: Y. Bilbille n, E. Gustilo n, K. Harris  n, C. Jones *, H. Lusic n, R. Kaiser*, M. Delaney*, L. Spremulli*, A. Deiters n, P. Agris  n

author keywords: 5-formylcytidine; NMR structure; mitochondrial translation; codon expansion; near cognate decoding
MeSH headings : Animals; Anticodon / chemistry; Anticodon / genetics; Base Pairing; Base Sequence; Cattle; Cytidine / analogs & derivatives; Cytidine / chemistry; Cytidine / genetics; Escherichia coli / genetics; Humans; Mitochondria / chemistry; Mitochondria / genetics; Molecular Sequence Data; RNA, Transfer, Met / chemistry; RNA, Transfer, Met / genetics; Ribosomes / chemistry; Ribosomes / genetics; Structure-Activity Relationship
topics (OpenAlex): RNA and protein synthesis mechanisms; RNA modifications and cancer; Genomics and Phylogenetic Studies
TL;DR: The physicochemical properties of this one modification, f(5)C(34), expand codon recognition from the traditional AUG to the non-traditional, synonymous codons AUU and AUC as well as AUA, in the reassignment of universal codons in the mitochondria. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2009 article

Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteins via a Pyrrolysyl-tRNA Synthetase/tRNACUA Pair and Click Chemistry

Nguyen, D. P., Lusic, H., Neumann, H., Kapadnis, P. B., Deiters, A., & Chin, J. W. (2009, June 10). Journal of the American Chemical Society.

By: D. Nguyen  n, H. Lusic n, H. Neumann  n, P. Kapadnis n, A. Deiters n & J. Chin  n

MeSH headings : Alkynes / chemistry; Alkynes / metabolism; Amino Acyl-tRNA Synthetases / genetics; Amino Acyl-tRNA Synthetases / metabolism; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Azides / chemistry; Azides / metabolism; Escherichia coli / genetics; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Lysine / chemistry; Lysine / genetics; Lysine / metabolism; Methanosarcina barkeri / enzymology; Myoglobin / chemistry; Myoglobin / genetics; Myoglobin / metabolism; Protein Engineering / methods; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism
topics (OpenAlex): Click Chemistry and Applications; RNA and protein synthesis mechanisms; Chemical Synthesis and Analysis
TL;DR: It is demonstrated that an orthogonal Methanosarcina barkeri MS pyrrolysyl-tRNA synthetase/tRNA(CUA) pair directs the efficient, site-specific incorporation of N6-[(2-propynyloxy)carbonyl]-L-lysine, containing a carbon-carbon triple bond, into recombinant proteins in Escherichia coli. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2009 journal article

Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization

Nucleic Acids Research, 37(8).

By: D. Young, H. Lusic, M. Lively & A. Deiters

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Gene Silencing in Mammalian Cells with Light-Activated Antisense Agents

ChemBioChem, 9(18), 2937–2940.

By: D. Young n, H. Lusic n, M. Lively*, J. Yoder*  & A. Deiters n

author keywords: Antisense agents; caged compounds; DNA; gene regulation; RNA
MeSH headings : Animals; Base Sequence; Gene Silencing; Genes, Reporter; Light; Luciferases, Renilla / chemistry; Mice; NIH 3T3 Cells; Oligonucleotides, Antisense / chemistry; Oligonucleotides, Antisense / metabolism; Oligonucleotides, Antisense / radiation effects; Phosphorothioate Oligonucleotides / chemistry; Phosphorothioate Oligonucleotides / metabolism; Transfection
topics (OpenAlex): Photochromic and Fluorescence Chemistry; bioluminescence and chemiluminescence research; Advanced biosensing and bioanalysis techniques
TL;DR: Detailed knowledge of the external regulation of gene func-tion is a fundamental necessity in order to annotate sequencedgenomes and to understand biological processes in single cells and multicellular organisms. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2008 article

Light-activated deoxyguanosine: photochemical regulation of peroxidase activity

Lusic, H., Lively, M. O., & Deiters, A. (2008, April 8). Molecular BioSystems.

By: H. Lusic n, M. Lively* & A. Deiters n

MeSH headings : Biocatalysis / radiation effects; DNA, Catalytic / chemical synthesis; DNA, Catalytic / chemistry; DNA, Catalytic / radiation effects; Deoxyguanosine / chemical synthesis; Deoxyguanosine / chemistry; Deoxyguanosine / metabolism; Enzyme Activation / radiation effects; Molecular Structure; Organophosphorus Compounds / chemical synthesis; Organophosphorus Compounds / chemistry; Organophosphorus Compounds / metabolism; Peroxidase / metabolism; Photochemistry; Time Factors; Ultraviolet Rays
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; Photochromic and Fluorescence Chemistry; Nanoplatforms for cancer theranostics
TL;DR: Photochemical activation of a deoxyribozyme with peroxidase activity was achieved by the synthesis and incorporation of a caged deoxyguanosine. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2008 article

Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet

Lusic, H., Gustilo, E. M., Vendeix, F. A. P., Kaiser, R., Delaney, M. O., Graham, W. D., … Deiters, A. (2008, October 16). Nucleic Acids Research.

By: H. Lusic*, E. Gustilo n, F. Vendeix n, R. Kaiser n, M. Delaney n, W. Graham n, V. Moye n, W. Cantara n, P. Agris n, A. Deiters n

MeSH headings : Anticodon / chemistry; Base Sequence; Codon / metabolism; Cytidine / analogs & derivatives; Cytidine / chemistry; Humans; Molecular Sequence Data; Nucleic Acid Conformation; Protein Biosynthesis; RNA / chemistry; RNA, Mitochondrial; RNA, Transfer, Met / chemical synthesis; RNA, Transfer, Met / chemistry; RNA, Transfer, Met / metabolism; Thermodynamics
topics (OpenAlex): RNA modifications and cancer; RNA and protein synthesis mechanisms; RNA Research and Splicing
TL;DR: The first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification are reported, contributing to the tRNAs anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2007 article

Light-triggered polymerase chain reaction

Young, D. D., Edwards, W. F., Lusic, H., Lively, M. O., & Deiters, A. (2007, November 14). Chemical Communications.

By: D. Young n, W. Edwards n, H. Lusic n, M. Lively* & A. Deiters n

MeSH headings : Base Sequence; DNA / chemistry; DNA Primers; Light; Photochemistry; Polymerase Chain Reaction / methods
topics (OpenAlex): Photochromic and Fluorescence Chemistry; DNA and Nucleic Acid Chemistry; Click Chemistry and Applications
TL;DR: Photochemical control of the polymerase chain reaction has been achieved through the incorporation of light-triggered nucleotides into DNA. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2007 article

Photochemical DNA Activation

Lusic, H., Young, D. D., Lively, M. O., & Deiters, A. (2007, April 21). Organic Letters.

By: H. Lusic n, D. Young n, M. Lively n & A. Deiters n

MeSH headings : DNA, Catalytic / chemistry; DNA, Catalytic / metabolism; Molecular Sequence Data; Molecular Structure; Photochemistry; RNA / metabolism; Substrate Specificity
topics (OpenAlex): Advanced biosensing and bioanalysis techniques; DNA and Nucleic Acid Chemistry; Photochromic and Fluorescence Chemistry
TL;DR: A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions and Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 article

A New Photocaging Group for Aromatic N-Heterocycles

Deiters, A., & Lusic, H. (2006, July 1). Synthesis.

By: A. Deiters* & H. Lusic*

author keywords: bioorganic chemistry; heterocycles; photocaging; protecting groups
topics (OpenAlex): Photochromic and Fluorescence Chemistry; Radical Photochemical Reactions; bioluminescence and chemiluminescence research
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Source: Web Of Science
Added: August 6, 2018

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