Hari Prakash Dwivedi

Works (7)

Updated: August 24th, 2023 21:22

2014 journal article

Selection and characterization of DNA aptamers specific for Listeria species

ANALYTICAL BIOCHEMISTRY, 459, 39–45.

By: S. Suh n, H. Dwivedi n, S. Choi n & L. Jaykus n

author keywords: Listeria monocytogenes; Listeria spp.; Aptamer; SELEX; Pathogen detection
MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / metabolism; Base Sequence; Binding Sites; Cell Survival; Listeria monocytogenes / cytology; Listeria monocytogenes / metabolism; SELEX Aptamer Technique / methods; Species Specificity; Substrate Specificity
TL;DR: This is the first study in which SELEX targeted bacterial cells at different growth phases and found that aptamers that bind to different cell surface moieties could increase the analytical sensitivity of future capture and detection assays. (via Semantic Scholar)
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Added: August 6, 2018

2014 journal article

Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains

PLOS ONE, 9(9).

By: B. Escudero-Abarca n, S. Suh n, M. Moore n, H. Dwivedi n & L. Jaykus n

MeSH headings : Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / metabolism; Bacteriological Techniques / methods; DNA, Viral / chemistry; DNA, Viral / metabolism; Feces / virology; Food Microbiology; Humans; Lettuce / virology; Molecular Structure; Norovirus / classification; Norovirus / genetics; Norovirus / isolation & purification; Sensitivity and Specificity
TL;DR: These ssDNA aptamer candidates show promise as broadly reactive reagents for use in HuNoV capture and detection assays in various sample types. (via Semantic Scholar)
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2013 journal article

Development and evaluation of aptamer magnetic capture assay in conjunction with real-time PCR for detection of Campylobacter jejuni

LWT-FOOD SCIENCE AND TECHNOLOGY, 56(2), 256–260.

By: S. Suh n, H. Dwivedi  n & L. Jaykus n

author keywords: Aptamer; Aptamer magnetic capture; Campylobacter jejuni; Real-time PCR; Pathogen detection
TL;DR: A prototype method for the concentration and detection of Campylobacter jejuni was developed using a previously reported biotinylated DNA aptamer in conjunction with qPCR, and nucleic acid aptamers are promising alternatives to antibodies for magnetic bead-based capture followed byqPCR detection. (via Semantic Scholar)
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2013 journal article

Selection of DNA aptamers for capture and detection of Salmonella Typhimurium using a whole-cell SELEX approach in conjunction with cell sorting

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97(8), 3677–3686.

By: H. Dwivedi n, R. Smiley n & L. Jaykus n

author keywords: Salmonella; DNA aptamers; Real-time PCR; Whole-cell SELEX
MeSH headings : Aptamers, Nucleotide; Bacteriological Techniques / methods; Cross Reactions; Flow Cytometry / methods; Food Microbiology / methods; Salmonella typhimurium / isolation & purification; Sensitivity and Specificity
TL;DR: This study provides proof-of-concept that biotinylated aptamers selected by whole-cell SELEX can be used in a qPCR-based capture-detection platform for Salmonella Typhimurium. (via Semantic Scholar)
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2010 review

Detection of pathogens in foods: the current state-of-the-art and future directions

[Review of ]. CRITICAL REVIEWS IN MICROBIOLOGY, 37(1), 40–63.

By: H. Dwivedi n & L. Jaykus n

MeSH headings : Bacteria / genetics; Bacteria / isolation & purification; Bacteriological Techniques / economics; DNA, Bacterial / genetics; Enzyme-Linked Immunosorbent Assay; Food Microbiology / economics; Food Microbiology / methods; Nucleic Acid Amplification Techniques; Polymerase Chain Reaction
TL;DR: This article reviews recent advancements in food borne pathogen detection and discusses future methods with a focus on pre-analytical sample processing, culture independent methods, and biosensors. (via Semantic Scholar)
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Added: August 6, 2018

2010 journal article

Selection and characterization of DNA aptamers with binding selectivity to Campylobacter jejuni using whole-cell SELEX

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(6), 2323–2334.

By: H. Dwivedi n, R. Smiley n & L. Jaykus n

author keywords: Food-borne pathogen; Molecular-based assay; Rapid microbial method; Aptamers
MeSH headings : Animals; Aptamers, Nucleotide / chemistry; Aptamers, Nucleotide / genetics; Campylobacter jejuni / chemistry; Campylobacter jejuni / genetics; Campylobacter jejuni / isolation & purification; Kinetics; Nucleic Acid Conformation; Poultry / microbiology; SELEX Aptamer Technique / methods; Species Specificity
TL;DR: Whole-cell SELEX is a promising technique to design aptamer-based molecular probes for microbial pathogens without tedious isolation and purification of complex markers or targets. (via Semantic Scholar)
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2008 journal article

Selection, characterization, and application of DNA aptamers for the capture and detection of Salmonella enterica serovars

MOLECULAR AND CELLULAR PROBES, 23(1), 20–28.

By: R. Joshi *, H. Janagama*, H. Dwivedi n, T. Kumar *, L. Jaykus n, J. Schefers*, S. Sreevatsan *

author keywords: Salmonella; Pre-analytical processing; Aptamers; Food borne pathogens; Detection
MeSH headings : Animals; Aptamers, Nucleotide / genetics; Aptamers, Nucleotide / isolation & purification; Base Sequence; Blotting, Western; Chickens; DNA Footprinting; Deoxyribonucleases / metabolism; Electrophoretic Mobility Shift Assay; Mass Spectrometry; Membrane Proteins / chemistry; Molecular Sequence Data; SELEX Aptamer Technique; Salmonella enterica / classification; Salmonella enterica / genetics; Salmonella enterica / isolation & purification
TL;DR: The applicability of Salmonella specific aptamers for pre-analytical sample processing as applied to food and environmental sample matrices is demonstrated. (via Semantic Scholar)
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